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The map label for this gene is tuf-1 [H]

Identifier: 88658644

GI number: 88658644

Start: 983263

End: 984450

Strand: Reverse

Name: tuf-1 [H]

Synonym: ECH_0960

Alternate gene names: 88658644

Gene position: 984450-983263 (Counterclockwise)

Preceding gene: 88658102

Following gene: 88657595

Centisome position: 83.69

GC content: 35.44

Gene sequence:

>1188_bases
ATGGTAGAAGAAAGGAAACCACATATAAATGTAGGGACAATAGGGCATGTTGATCATGGTAAGACAACATTAACAGCAGC
GTTAACAACAGTGTTAGCGAAGAGGTTGAGTGGAGAAGGTAATAAAAGTGTAAAGTATGACGAAATAGATAAAGCACCAG
AAGAGAAAGCAAGAGGAATAACCATTTCAACAGCACATGTAGAATATGAAACAGAGAATAGACATTATGCGCATGTAGAT
TGTCCAGGACATGCTGATTATATAAAAAATATGATAACAGGTGCTGCACAAATGGATGCAGCAATATTAGTAGTATCTGC
AACAGATGGAGCAATGCCACAAACAAGAGAACATATACTATTAGCAAAACAAGTTGGAGTAAAAGATATAGTAGTATGGA
TGAATAAGTGTGATGTTGTAGATGATGAAGAAATGTTGTCATTAGTTGAGATGGAAATAAGAGAATTATTATCAAAATAT
GGATATCCAGGAGATGATATAGATGTTGTAAGAGGATCTGCAGTTAAGGCATTGGAAGAAGAAACAGGTTCAGGAGTCTG
GAGTGAAAAAATAATGGAATTGATGAATGCCTTAGAAAAAATAAGTTTACCAACAAGAGAAAAAGATAAGCCATTTTTAA
TGTCAATAGAAGATGTATTTTCAATACCTGGAAGAGGTACAGTAGTAACAGGAAGAATAGAGAGAGGAGTAATTAGAGTA
GGAGATAAAATAGAGATAGTAGGGTTACGAGATATACAGAGCACAGTATGTACAGGTGTTGAAATGTTTCATAAAGCATT
AGATGCAGGAGAAGCAGGGGATAATGCAGGTATATTGTTAAGAGGAATAAAGAAAGAGGATGTAGAGAGAGGGCAAGTAT
TGAGTGCGCCTGGTCAAATACATTCGTATAAGAAATTTAAAGCAGAGGTATATATATTAAAGAAAGAAGAGGGAGGAAGG
CATACACCGTTTTTCTCAAATTATCAGCCACAATTTTATGTTAGAACAACTGATGTAACAGGTAGTATAAAATTACCTGA
GGGGGTAGAGATGGTAATGCCTGGGGATAATATAAGTATCGAAGTAAGTTTAGATAAGCCTGTTGCTATTGATAAAGGAT
TGAGATTTGCAATTCGTGAAGGTGGTAGAACTGTAGGCTCTGGTATTATTACTGAAATTTTGGAGTAG

Upstream 100 bases:

>100_bases
CAGGGTAGAGCACAATATAGCATGTATTTTTCTTGTTATGAACAAGTACCTGATAATATAGTGGCAAACGAGATAAAAAC
TAAATAATAGGTGTAATGTT

Downstream 100 bases:

>100_bases
TGATATATTGATGTTTAATAGGAGTGTAGCTCAATTGGCTAGAGCGTCAGTCTCCAAAACTGAAGGTTATGGGTTCGATT
CCTATCACTCCTGCCGTTTA

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MVEERKPHINVGTIGHVDHGKTTLTAALTTVLAKRLSGEGNKSVKYDEIDKAPEEKARGITISTAHVEYETENRHYAHVD
CPGHADYIKNMITGAAQMDAAILVVSATDGAMPQTREHILLAKQVGVKDIVVWMNKCDVVDDEEMLSLVEMEIRELLSKY
GYPGDDIDVVRGSAVKALEEETGSGVWSEKIMELMNALEKISLPTREKDKPFLMSIEDVFSIPGRGTVVTGRIERGVIRV
GDKIEIVGLRDIQSTVCTGVEMFHKALDAGEAGDNAGILLRGIKKEDVERGQVLSAPGQIHSYKKFKAEVYILKKEEGGR
HTPFFSNYQPQFYVRTTDVTGSIKLPEGVEMVMPGDNISIEVSLDKPVAIDKGLRFAIREGGRTVGSGIITEILE

Sequences:

>Translated_395_residues
MVEERKPHINVGTIGHVDHGKTTLTAALTTVLAKRLSGEGNKSVKYDEIDKAPEEKARGITISTAHVEYETENRHYAHVD
CPGHADYIKNMITGAAQMDAAILVVSATDGAMPQTREHILLAKQVGVKDIVVWMNKCDVVDDEEMLSLVEMEIRELLSKY
GYPGDDIDVVRGSAVKALEEETGSGVWSEKIMELMNALEKISLPTREKDKPFLMSIEDVFSIPGRGTVVTGRIERGVIRV
GDKIEIVGLRDIQSTVCTGVEMFHKALDAGEAGDNAGILLRGIKKEDVERGQVLSAPGQIHSYKKFKAEVYILKKEEGGR
HTPFFSNYQPQFYVRTTDVTGSIKLPEGVEMVMPGDNISIEVSLDKPVAIDKGLRFAIREGGRTVGSGIITEILE
>Mature_395_residues
MVEERKPHINVGTIGHVDHGKTTLTAALTTVLAKRLSGEGNKSVKYDEIDKAPEEKARGITISTAHVEYETENRHYAHVD
CPGHADYIKNMITGAAQMDAAILVVSATDGAMPQTREHILLAKQVGVKDIVVWMNKCDVVDDEEMLSLVEMEIRELLSKY
GYPGDDIDVVRGSAVKALEEETGSGVWSEKIMELMNALEKISLPTREKDKPFLMSIEDVFSIPGRGTVVTGRIERGVIRV
GDKIEIVGLRDIQSTVCTGVEMFHKALDAGEAGDNAGILLRGIKKEDVERGQVLSAPGQIHSYKKFKAEVYILKKEEGGR
HTPFFSNYQPQFYVRTTDVTGSIKLPEGVEMVMPGDNISIEVSLDKPVAIDKGLRFAIREGGRTVGSGIITEILE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=391, Percent_Identity=54.9872122762148, Blast_Score=447, Evalue=1e-125,
Organism=Homo sapiens, GI4503475, Length=450, Percent_Identity=30.6666666666667, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI4503471, Length=441, Percent_Identity=30.8390022675737, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI223555963, Length=433, Percent_Identity=29.5612009237875, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI5729864, Length=433, Percent_Identity=29.5612009237875, Blast_Score=165, Evalue=9e-41,
Organism=Homo sapiens, GI54607086, Length=328, Percent_Identity=29.8780487804878, Blast_Score=138, Evalue=9e-33,
Organism=Homo sapiens, GI194018522, Length=336, Percent_Identity=31.25, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI194018520, Length=336, Percent_Identity=31.25, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI194097354, Length=336, Percent_Identity=31.25, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI46094014, Length=336, Percent_Identity=30.6547619047619, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI4503507, Length=283, Percent_Identity=28.2685512367491, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI82546879, Length=431, Percent_Identity=23.8979118329466, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI94966754, Length=136, Percent_Identity=33.0882352941176, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI157426893, Length=240, Percent_Identity=28.75, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI45593140, Length=432, Percent_Identity=23.6111111111111, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1790412, Length=393, Percent_Identity=68.9567430025445, Blast_Score=540, Evalue=1e-155,
Organism=Escherichia coli, GI1789737, Length=393, Percent_Identity=68.9567430025445, Blast_Score=540, Evalue=1e-155,
Organism=Escherichia coli, GI2367247, Length=377, Percent_Identity=28.9124668435013, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1789108, Length=261, Percent_Identity=29.8850574712644, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI48994988, Length=289, Percent_Identity=29.0657439446367, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788922, Length=246, Percent_Identity=28.0487804878049, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=396, Percent_Identity=52.020202020202, Blast_Score=391, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI25141371, Length=390, Percent_Identity=45.1282051282051, Blast_Score=335, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI17552884, Length=441, Percent_Identity=29.7052154195011, Blast_Score=164, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17569207, Length=441, Percent_Identity=29.7052154195011, Blast_Score=164, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI32566303, Length=429, Percent_Identity=28.2051282051282, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI32566629, Length=346, Percent_Identity=30.9248554913295, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI115532067, Length=437, Percent_Identity=27.0022883295195, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI115532065, Length=437, Percent_Identity=27.0022883295195, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17506081, Length=249, Percent_Identity=31.3253012048193, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=145, Percent_Identity=42.7586206896552, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17557151, Length=236, Percent_Identity=25.8474576271186, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17565438, Length=422, Percent_Identity=23.9336492890995, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324761, Length=399, Percent_Identity=59.1478696741855, Blast_Score=478, Evalue=1e-136,
Organism=Saccharomyces cerevisiae, GI6325337, Length=441, Percent_Identity=31.0657596371882, Blast_Score=168, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6319594, Length=441, Percent_Identity=31.0657596371882, Blast_Score=168, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6322937, Length=461, Percent_Identity=27.114967462039, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6320377, Length=354, Percent_Identity=29.0960451977401, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=277, Percent_Identity=27.0758122743682, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=241, Percent_Identity=29.045643153527, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=56.7430025445293, Blast_Score=456, Evalue=1e-128,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=56.7430025445293, Blast_Score=456, Evalue=1e-128,
Organism=Drosophila melanogaster, GI19921738, Length=390, Percent_Identity=48.2051282051282, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI45553807, Length=442, Percent_Identity=30.316742081448, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI45553816, Length=442, Percent_Identity=30.316742081448, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24651721, Length=442, Percent_Identity=30.316742081448, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI17864154, Length=442, Percent_Identity=30.316742081448, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24652838, Length=441, Percent_Identity=29.9319727891156, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI17137572, Length=441, Percent_Identity=29.9319727891156, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI45550900, Length=436, Percent_Identity=28.2110091743119, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI17137380, Length=342, Percent_Identity=31.8713450292398, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI19922690, Length=310, Percent_Identity=30.6451612903226, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24647048, Length=369, Percent_Identity=26.5582655826558, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24647046, Length=369, Percent_Identity=26.5582655826558, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI28572034, Length=242, Percent_Identity=30.5785123966942, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: NA

Molecular weight: Translated: 43387; Mature: 43387

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEERKPHINVGTIGHVDHGKTTLTAALTTVLAKRLSGEGNKSVKYDEIDKAPEEKARGI
CCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCHHHHHCCE
TISTAHVEYETENRHYAHVDCPGHADYIKNMITGAAQMDAAILVVSATDGAMPQTREHIL
EEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCEEEEEEEECCCCCCCCHHHEE
LAKQVGVKDIVVWMNKCDVVDDEEMLSLVEMEIRELLSKYGYPGDDIDVVRGSAVKALEE
EHHHCCHHHEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHH
ETGSGVWSEKIMELMNALEKISLPTREKDKPFLMSIEDVFSIPGRGTVVTGRIERGVIRV
HCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHCCCCCCEEEEEEECCCEEEE
GDKIEIVGLRDIQSTVCTGVEMFHKALDAGEAGDNAGILLRGIKKEDVERGQVLSAPGQI
CCEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHCCCCEECCCCHH
HSYKKFKAEVYILKKEEGGRHTPFFSNYQPQFYVRTTDVTGSIKLPEGVEMVMPGDNISI
HHHHEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCEEE
EVSLDKPVAIDKGLRFAIREGGRTVGSGIITEILE
EEEECCCEECCCCCEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MVEERKPHINVGTIGHVDHGKTTLTAALTTVLAKRLSGEGNKSVKYDEIDKAPEEKARGI
CCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCHHHHHCCE
TISTAHVEYETENRHYAHVDCPGHADYIKNMITGAAQMDAAILVVSATDGAMPQTREHIL
EEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCEEEEEEEECCCCCCCCHHHEE
LAKQVGVKDIVVWMNKCDVVDDEEMLSLVEMEIRELLSKYGYPGDDIDVVRGSAVKALEE
EHHHCCHHHEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHH
ETGSGVWSEKIMELMNALEKISLPTREKDKPFLMSIEDVFSIPGRGTVVTGRIERGVIRV
HCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHCCCCCCEEEEEEECCCEEEE
GDKIEIVGLRDIQSTVCTGVEMFHKALDAGEAGDNAGILLRGIKKEDVERGQVLSAPGQI
CCEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHCCCCEECCCCHH
HSYKKFKAEVYILKKEEGGRHTPFFSNYQPQFYVRTTDVTGSIKLPEGVEMVMPGDNISI
HHHHEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCEEE
EVSLDKPVAIDKGLRFAIREGGRTVGSGIITEILE
EEEECCCEECCCCCEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA