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The map label for this gene is sspA [H]

Identifier: 88658632

GI number: 88658632

Start: 865324

End: 865989

Strand: Direct

Name: sspA [H]

Synonym: ECH_0847

Alternate gene names: 88658632

Gene position: 865324-865989 (Clockwise)

Preceding gene: 88657853

Following gene: 88658376

Centisome position: 73.57

GC content: 30.48

Gene sequence:

>666_bases
ATGGATACATTGTATCATTTTCCACTATGTCCTTTTTCCAGAAAAGTACGCATCTTTCTCAAAGAAAAAAAATTTAACTT
TCATCAGATAGAAGAAAACCCCTGGAAAAAACGTGAAGAGTTTATAAAAATCAACCCTGTATGTCAGGTCCCCGTATTAA
TCAGTGGACAACATGTGATTGCAGATAGCCAAGCAATTTGTGAATACATTGAAGAATTATATGATAGTATATCACTACTT
GGGAATTCACTATACATAAGGTCAACAGTGCGCAAGTTAATATACTGGATTGACTATAAATTTTATCATGAAATTACAAG
ATATATCATAAATGAAAAAGTATCAAAGTATTATATAAAAAATGCTAGTCCAGATTCTAGGTTTATCCAAGCAGCAAGAC
AAAATTTATTGCCTCATATAAGGTACATAGAAAGATTACTCAATAATAATAGTTGGATTGCTTGCAATGAATTTACTTTA
GCAGATATAACATTAGCTGCACACATATCAGTTTTAGATTTCATGAATATTTTTCCATGGCATTCAACAGCCATATTAAA
AGAATGGTATTCCGTAATAAAGTCTAAACCATCATTTAGCGATATATTAAATGACAAAGTAATAGGTATAACACCTCCTG
TACATTACAATCAACTGGATTTCTAA

Upstream 100 bases:

>100_bases
TATCTATCTGTAATTCTATAAGATAAGTCTAAAAATATTACATTGCACAAGGTTACCAAGTCTGTTATAAATTAAACCAT
TCGTATGCATGAAATTTACT

Downstream 100 bases:

>100_bases
ACTATGCTTTTCTTATTTTTTAACTAGGTGAAGTACACTAGGTATTAATAAAAATATTATAAATTTATGCAATTACAAAA
TAAAATGTATAACATACAAT

Product: glutathione S-transferase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MDTLYHFPLCPFSRKVRIFLKEKKFNFHQIEENPWKKREEFIKINPVCQVPVLISGQHVIADSQAICEYIEELYDSISLL
GNSLYIRSTVRKLIYWIDYKFYHEITRYIINEKVSKYYIKNASPDSRFIQAARQNLLPHIRYIERLLNNNSWIACNEFTL
ADITLAAHISVLDFMNIFPWHSTAILKEWYSVIKSKPSFSDILNDKVIGITPPVHYNQLDF

Sequences:

>Translated_221_residues
MDTLYHFPLCPFSRKVRIFLKEKKFNFHQIEENPWKKREEFIKINPVCQVPVLISGQHVIADSQAICEYIEELYDSISLL
GNSLYIRSTVRKLIYWIDYKFYHEITRYIINEKVSKYYIKNASPDSRFIQAARQNLLPHIRYIERLLNNNSWIACNEFTL
ADITLAAHISVLDFMNIFPWHSTAILKEWYSVIKSKPSFSDILNDKVIGITPPVHYNQLDF
>Mature_221_residues
MDTLYHFPLCPFSRKVRIFLKEKKFNFHQIEENPWKKREEFIKINPVCQVPVLISGQHVIADSQAICEYIEELYDSISLL
GNSLYIRSTVRKLIYWIDYKFYHEITRYIINEKVSKYYIKNASPDSRFIQAARQNLLPHIRYIERLLNNNSWIACNEFTL
ADITLAAHISVLDFMNIFPWHSTAILKEWYSVIKSKPSFSDILNDKVIGITPPVHYNQLDF

Specific function: Forms an equimolar complex with the RNA polymerase holoenzyme (RNAP) but not with the core enzyme [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

None

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: NA

Molecular weight: Translated: 26240; Mature: 26240

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTLYHFPLCPFSRKVRIFLKEKKFNFHQIEENPWKKREEFIKINPVCQVPVLISGQHVI
CCCCCCCCCCCCCCCEEEEEEECCCCCEECCCCCCHHHHCEEEECCCEECCEEECCCEEE
ADSQAICEYIEELYDSISLLGNSLYIRSTVRKLIYWIDYKFYHEITRYIINEKVSKYYIK
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NASPDSRFIQAARQNLLPHIRYIERLLNNNSWIACNEFTLADITLAAHISVLDFMNIFPW
CCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCC
HSTAILKEWYSVIKSKPSFSDILNDKVIGITPPVHYNQLDF
CHHHHHHHHHHHHHCCCCHHHHHCCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MDTLYHFPLCPFSRKVRIFLKEKKFNFHQIEENPWKKREEFIKINPVCQVPVLISGQHVI
CCCCCCCCCCCCCCCEEEEEEECCCCCEECCCCCCHHHHCEEEECCCEECCEEECCCEEE
ADSQAICEYIEELYDSISLLGNSLYIRSTVRKLIYWIDYKFYHEITRYIINEKVSKYYIK
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NASPDSRFIQAARQNLLPHIRYIERLLNNNSWIACNEFTLADITLAAHISVLDFMNIFPW
CCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCC
HSTAILKEWYSVIKSKPSFSDILNDKVIGITPPVHYNQLDF
CHHHHHHHHHHHHHCCCCHHHHHCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12704232 [H]