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The map label for this gene is birA [H]

Identifier: 88658376

GI number: 88658376

Start: 866128

End: 866883

Strand: Direct

Name: birA [H]

Synonym: ECH_0848

Alternate gene names: 88658376

Gene position: 866128-866883 (Clockwise)

Preceding gene: 88658632

Following gene: 88657663

Centisome position: 73.63

GC content: 29.76

Gene sequence:

>756_bases
ATGAAACTAAACCTATCTAATATAAATCTACCAAACGGATATACTTTAGAACATCACAAAATTTTATATAGTACAAATTA
CGAAGCAATCAAACTTGCAGAATGCGGAGCTGAAGATGGTACAATAATCATATCCGATAAACAAATTAAAGGTAGAGGAA
GATATGAGCGCACATGGTACTCCCCCACAGGCAATTTATATTTCAGTATTATTATAACAAAATTCAAAGAAACGTTCCCT
TTACCATTTATTGCAGCATTATCTGTTGGAAAAACACTGGAAGAAATATTAATAAATAACAACAACACAACATCAAAAGT
AGAATATAAATGGCCCAATGATATATTAGTAAATAGTAAAAAAATATCAGGTATATTGTTAGAAAGTAAAACATTGAATA
ACACACTACAAAAATGGATAGTAGTCGGCATAGGCATCAATATGTATAAAGCACCAACATATGCAACAACAATCAAGGAG
CATAATCAAAATTTCACCCTATCTAACTTAGAATTACTGGAAAAAGTAATAAATAATTTTTACAACTTCAGAAAGCATTA
TATTTATCACGGGTTTGCAGATATAAGGAACCTATGGCTCAAACAAACATGTCATTTAAACAGAGAAATAACTGTTAAAA
GCTACAAACACACTTACACTGGTCAATTCATTACAATAGATACACAAGGTAACATGTTAATGAAACAAAACGATAAAACA
ATATGCATCACCTCAGGAGAAATATTTAATATCTAA

Upstream 100 bases:

>100_bases
TAGGTATTAATAAAAATATTATAAATTTATGCAATTACAAAATAAAATGTATAACATACAATAGTCTGTAATTTTATAAG
CACTATAAAACTTTATTAAT

Downstream 100 bases:

>100_bases
ACCTAGATGATTTATAACAATCTAATAATAAGACATATGTAACACACCATTTATAATATAAAAAACGCTAAGCTAGTTAT
ACTTAATGTAACATAAAATT

Product: biotin--acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin--protein ligase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MKLNLSNINLPNGYTLEHHKILYSTNYEAIKLAECGAEDGTIIISDKQIKGRGRYERTWYSPTGNLYFSIIITKFKETFP
LPFIAALSVGKTLEEILINNNNTTSKVEYKWPNDILVNSKKISGILLESKTLNNTLQKWIVVGIGINMYKAPTYATTIKE
HNQNFTLSNLELLEKVINNFYNFRKHYIYHGFADIRNLWLKQTCHLNREITVKSYKHTYTGQFITIDTQGNMLMKQNDKT
ICITSGEIFNI

Sequences:

>Translated_251_residues
MKLNLSNINLPNGYTLEHHKILYSTNYEAIKLAECGAEDGTIIISDKQIKGRGRYERTWYSPTGNLYFSIIITKFKETFP
LPFIAALSVGKTLEEILINNNNTTSKVEYKWPNDILVNSKKISGILLESKTLNNTLQKWIVVGIGINMYKAPTYATTIKE
HNQNFTLSNLELLEKVINNFYNFRKHYIYHGFADIRNLWLKQTCHLNREITVKSYKHTYTGQFITIDTQGNMLMKQNDKT
ICITSGEIFNI
>Mature_251_residues
MKLNLSNINLPNGYTLEHHKILYSTNYEAIKLAECGAEDGTIIISDKQIKGRGRYERTWYSPTGNLYFSIIITKFKETFP
LPFIAALSVGKTLEEILINNNNTTSKVEYKWPNDILVNSKKISGILLESKTLNNTLQKWIVVGIGINMYKAPTYATTIKE
HNQNFTLSNLELLEKVINNFYNFRKHYIYHGFADIRNLWLKQTCHLNREITVKSYKHTYTGQFITIDTQGNMLMKQNDKT
ICITSGEIFNI

Specific function: Activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins [H]

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=246, Percent_Identity=27.6422764227642, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR003142
- InterPro:   IPR004143 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 29003; Mature: 29003

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNLSNINLPNGYTLEHHKILYSTNYEAIKLAECGAEDGTIIISDKQIKGRGRYERTWY
CEEECCCCCCCCCCEEECEEEEEECCCCEEEEEECCCCCCEEEEECCEECCCCCEEEEEE
SPTGNLYFSIIITKFKETFPLPFIAALSVGKTLEEILINNNNTTSKVEYKWPNDILVNSK
CCCCCEEEEEEEEEHHHHCCCCHHHHHHHHHHHHHHEECCCCCCEEEEEECCCCEEEECC
KISGILLESKTLNNTLQKWIVVGIGINMYKAPTYATTIKEHNQNFTLSNLELLEKVINNF
EEEEEEEECHHHHHHHHEEEEEEECCEEEECCCEEEHHHHCCCCCEEHHHHHHHHHHHHH
YNFRKHYIYHGFADIRNLWLKQTCHLNREITVKSYKHTYTGQFITIDTQGNMLMKQNDKT
HHHHHHEEEECHHHHHHHHHHHHHCCCCEEEEEEECEEECCEEEEEECCCCEEEEECCCE
ICITSGEIFNI
EEEECCCEECC
>Mature Secondary Structure
MKLNLSNINLPNGYTLEHHKILYSTNYEAIKLAECGAEDGTIIISDKQIKGRGRYERTWY
CEEECCCCCCCCCCEEECEEEEEECCCCEEEEEECCCCCCEEEEECCEECCCCCEEEEEE
SPTGNLYFSIIITKFKETFPLPFIAALSVGKTLEEILINNNNTTSKVEYKWPNDILVNSK
CCCCCEEEEEEEEEHHHHCCCCHHHHHHHHHHHHHHEECCCCCCEEEEEECCCCEEEECC
KISGILLESKTLNNTLQKWIVVGIGINMYKAPTYATTIKEHNQNFTLSNLELLEKVINNF
EEEEEEEECHHHHHHHHEEEEEEECCEEEECCCEEEHHHHCCCCCEEHHHHHHHHHHHHH
YNFRKHYIYHGFADIRNLWLKQTCHLNREITVKSYKHTYTGQFITIDTQGNMLMKQNDKT
HHHHHHEEEECHHHHHHHHHHHHHCCCCEEEEEEECEEECCEEEEEECCCCEEEEECCCE
ICITSGEIFNI
EEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]