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The map label for this gene is rnhB

Identifier: 88658621

GI number: 88658621

Start: 968499

End: 969128

Strand: Direct

Name: rnhB

Synonym: ECH_0946

Alternate gene names: 88658621

Gene position: 968499-969128 (Clockwise)

Preceding gene: 88658155

Following gene: 88658371

Centisome position: 82.34

GC content: 32.38

Gene sequence:

>630_bases
ATGCCAGATTTTTCTATAGAAAATGAAATATCAAAATTAATAAATAATAAACACTGTACTATAGTAGGAGTTGACGAAGT
AGGATATGGTTCATTAGCTGGCCCTGTAGTATCAGCTGCTGTTTTTTTTCCAAAACATGACAATCACATCACATACAATA
TTCAAGATTCAAAAAAACTCACTCCAAAAAAAAGGCTAGAAATTTATAATATAATCACTCCTATGGTAAAATGGAGTATA
GGGCTTGCTGAAGTGCATGAAATTGACCAGTATAATATACTAAATGCTACACATATTGCTATGCAAAGGGCACTTAGAGG
GTTAAATTCTAATATAGATTATGTAATAGTAGATGGAAATAAAGTTCCAGAGCTACCATGGAATGCAAAAGCCGTTGTAG
ATGGAGATAACATAAGTATATCAATTGCAGCAGCATCTATAATTGCAAAAGTCACACGAGATAAACTAATGGAAACACTA
CACATACAATTCCCACAATACAACTGGAACAAAAATAAAGGGTATGGTACTAAACATCATTTAGAGTCTTTACATAAGTA
TGGTAAAACTATACACCACAGAAATACATTTGCCCCAGCATCAGGGATAACTAAACTTTACAATAAATGA

Upstream 100 bases:

>100_bases
AATCCTCTACCTTTAAACGCTAAAAAACTTTCTTCACAAGCAAATCTAAAGTATAAATATACTTTACAACTAATCCTTAC
TAAAACCTAAAATAAGCTTT

Downstream 100 bases:

>100_bases
TAAAAACCTCACTACTATTGATTTAAAACACACTCTATAAATTCACACTATACTCTAAATACCACCTATTTAATCAACAC
TGTATAAAAGTACTCAACTT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MPDFSIENEISKLINNKHCTIVGVDEVGYGSLAGPVVSAAVFFPKHDNHITYNIQDSKKLTPKKRLEIYNIITPMVKWSI
GLAEVHEIDQYNILNATHIAMQRALRGLNSNIDYVIVDGNKVPELPWNAKAVVDGDNISISIAAASIIAKVTRDKLMETL
HIQFPQYNWNKNKGYGTKHHLESLHKYGKTIHHRNTFAPASGITKLYNK

Sequences:

>Translated_209_residues
MPDFSIENEISKLINNKHCTIVGVDEVGYGSLAGPVVSAAVFFPKHDNHITYNIQDSKKLTPKKRLEIYNIITPMVKWSI
GLAEVHEIDQYNILNATHIAMQRALRGLNSNIDYVIVDGNKVPELPWNAKAVVDGDNISISIAAASIIAKVTRDKLMETL
HIQFPQYNWNKNKGYGTKHHLESLHKYGKTIHHRNTFAPASGITKLYNK
>Mature_208_residues
PDFSIENEISKLINNKHCTIVGVDEVGYGSLAGPVVSAAVFFPKHDNHITYNIQDSKKLTPKKRLEIYNIITPMVKWSIG
LAEVHEIDQYNILNATHIAMQRALRGLNSNIDYVIVDGNKVPELPWNAKAVVDGDNISISIAAASIIAKVTRDKLMETLH
IQFPQYNWNKNKGYGTKHHLESLHKYGKTIHHRNTFAPASGITKLYNK

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=180, Percent_Identity=51.1111111111111, Blast_Score=176, Evalue=8e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_EHRCR (Q2GFP8)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507735.1
- ProteinModelPortal:   Q2GFP8
- SMR:   Q2GFP8
- STRING:   Q2GFP8
- GeneID:   3927326
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0946
- TIGR:   ECH_0946
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- PhylomeDB:   Q2GFP8
- ProtClustDB:   PRK00015
- BioCyc:   ECHA205920:ECH_0946-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 23498; Mature: 23367

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDFSIENEISKLINNKHCTIVGVDEVGYGSLAGPVVSAAVFFPKHDNHITYNIQDSKKL
CCCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHEEECCCCCEEEEEECCCCCC
TPKKRLEIYNIITPMVKWSIGLAEVHEIDQYNILNATHIAMQRALRGLNSNIDYVIVDGN
CHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCC
KVPELPWNAKAVVDGDNISISIAAASIIAKVTRDKLMETLHIQFPQYNWNKNKGYGTKHH
CCCCCCCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHH
LESLHKYGKTIHHRNTFAPASGITKLYNK
HHHHHHHHHHHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
PDFSIENEISKLINNKHCTIVGVDEVGYGSLAGPVVSAAVFFPKHDNHITYNIQDSKKL
CCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHEEECCCCCEEEEEECCCCCC
TPKKRLEIYNIITPMVKWSIGLAEVHEIDQYNILNATHIAMQRALRGLNSNIDYVIVDGN
CHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCC
KVPELPWNAKAVVDGDNISISIAAASIIAKVTRDKLMETLHIQFPQYNWNKNKGYGTKHH
CCCCCCCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHH
LESLHKYGKTIHHRNTFAPASGITKLYNK
HHHHHHHHHHHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA