Click here to switch to the map view.

The map label for this gene is dnaN [H]

Identifier: 88658616

GI number: 88658616

Start: 1034984

End: 1036093

Strand: Reverse

Name: dnaN [H]

Synonym: ECH_1009

Alternate gene names: 88658616

Gene position: 1036093-1034984 (Counterclockwise)

Preceding gene: 88657845

Following gene: 88657674

Centisome position: 88.08

GC content: 28.65

Gene sequence:

>1110_bases
TTGAACTTTTTTGTTAGCAAAGATAAATTACTGCAAGCTTTGTCTTACATTAATAGTATTGTAGAAAAACGTCATAGTAT
TGTTGTATTGTCGTGTGTAAGAATAGAAGCAAGGGATCAAGAAATTAAGTTAACATCTACAGATCTTGATATTTCAATAA
TTGCATCTATTACTGCCCATGTAAACGTTAATGGAGTTGTAGTAGTGTCTGCTCAACTTCTTTATGATATTGTAAAAAAG
TTGCCTAATGATATTGAAATCAAATTTTTTATTGGTGAAGATGGAAGATTAACTATAAAATGTGGTCGTACGGAGTTTTT
TCTTCCTGTAGTTGATATAGAGAAGTTTCCTATTATTGAAAATAATGAATTACAAAACAAATTTACTTTGCAAGTCTCTG
AATTGGTTAGTTTGTTTGGTAAAACTAAATTTGCAATTTCCATAGAAGAGAGTAGATTTAATTTGAATGGATTATATGTT
CATACGTCCAATAATATCTTATGTTGTGCTGCTACAGATGGACATAGGTTGTCGTTAACTAAGTTGAGTCAATCTCAGAA
TGTATTGTCAAATTTTGGTATTATAGTTCCCAAAAAAACAGTTAATGAAATTTTGAAGATAGTAAGTCATGGTAGTGGTA
ATGATTTAATATCAATTAGTTTATCAGATAGGAAGATTTGTTTTGAGTATAAAGAATATATTTTAATATCTAAAGTAATA
GATGGTACTTTTCCGGATTATAATTCTATTATTCCTAGATCTCATGATAAGAATGTATTAATTGAATCTGAAACGTTATC
TAATGCGATAGATAGAGTATCTGTAGTAGTCTTTGATAAAGTAAAAGTGATAAAATTAAGGTTTGTGAGTAATAAACTTA
TATTAAGTGCTGCTTCTTCAGATCAAGGTGATGCTAGAGAGGAATTAGAAGTAGATTATTCTGGAGAAGACATGACTATT
GGTTTAAATGCCAGATATTTAATGGAAGCTTTGTATTGTATAAAAGGAAAATGTCTTTTATCGTTTTATGATGCTGGTAC
ATCTGTTTTAATACAGGAAGAAGAAAATATAGATGATTTCACTTACATTATTATGCCTATGAGAATATAG

Upstream 100 bases:

>100_bases
ATAGAGCAAAGTGATCCATACCTTGACTTGTTTAAGTTTAATTTGTATAACATCACAATGGGGTAGGTTTATATAGAAAT
ATATGAGTGATGGTATTAAG

Downstream 100 bases:

>100_bases
AATTTGTTATATTAAATTCTGTTAGTAGTTTTATATTATCTAAAATTGGCTGTTTGTTAAAAAAGGTGTCCTTAAAATTA
ATAAGGAAATATAAACACTC

Product: DNA polymerase III, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MNFFVSKDKLLQALSYINSIVEKRHSIVVLSCVRIEARDQEIKLTSTDLDISIIASITAHVNVNGVVVVSAQLLYDIVKK
LPNDIEIKFFIGEDGRLTIKCGRTEFFLPVVDIEKFPIIENNELQNKFTLQVSELVSLFGKTKFAISIEESRFNLNGLYV
HTSNNILCCAATDGHRLSLTKLSQSQNVLSNFGIIVPKKTVNEILKIVSHGSGNDLISISLSDRKICFEYKEYILISKVI
DGTFPDYNSIIPRSHDKNVLIESETLSNAIDRVSVVVFDKVKVIKLRFVSNKLILSAASSDQGDAREELEVDYSGEDMTI
GLNARYLMEALYCIKGKCLLSFYDAGTSVLIQEEENIDDFTYIIMPMRI

Sequences:

>Translated_369_residues
MNFFVSKDKLLQALSYINSIVEKRHSIVVLSCVRIEARDQEIKLTSTDLDISIIASITAHVNVNGVVVVSAQLLYDIVKK
LPNDIEIKFFIGEDGRLTIKCGRTEFFLPVVDIEKFPIIENNELQNKFTLQVSELVSLFGKTKFAISIEESRFNLNGLYV
HTSNNILCCAATDGHRLSLTKLSQSQNVLSNFGIIVPKKTVNEILKIVSHGSGNDLISISLSDRKICFEYKEYILISKVI
DGTFPDYNSIIPRSHDKNVLIESETLSNAIDRVSVVVFDKVKVIKLRFVSNKLILSAASSDQGDAREELEVDYSGEDMTI
GLNARYLMEALYCIKGKCLLSFYDAGTSVLIQEEENIDDFTYIIMPMRI
>Mature_369_residues
MNFFVSKDKLLQALSYINSIVEKRHSIVVLSCVRIEARDQEIKLTSTDLDISIIASITAHVNVNGVVVVSAQLLYDIVKK
LPNDIEIKFFIGEDGRLTIKCGRTEFFLPVVDIEKFPIIENNELQNKFTLQVSELVSLFGKTKFAISIEESRFNLNGLYV
HTSNNILCCAATDGHRLSLTKLSQSQNVLSNFGIIVPKKTVNEILKIVSHGSGNDLISISLSDRKICFEYKEYILISKVI
DGTFPDYNSIIPRSHDKNVLIESETLSNAIDRVSVVVFDKVKVIKLRFVSNKLILSAASSDQGDAREELEVDYSGEDMTI
GLNARYLMEALYCIKGKCLLSFYDAGTSVLIQEEENIDDFTYIIMPMRI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=373, Percent_Identity=28.9544235924933, Blast_Score=189, Evalue=3e-49,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41470; Mature: 41470

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFFVSKDKLLQALSYINSIVEKRHSIVVLSCVRIEARDQEIKLTSTDLDISIIASITAH
CCCEECHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCEEEEEECCCCEEEEEEEEEE
VNVNGVVVVSAQLLYDIVKKLPNDIEIKFFIGEDGRLTIKCGRTEFFLPVVDIEKFPIIE
EECCEEEEEEHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCEEEEEEEECCCCCCCCC
NNELQNKFTLQVSELVSLFGKTKFAISIEESRFNLNGLYVHTSNNILCCAATDGHRLSLT
CCCCCCEEEEEHHHHHHHHCCEEEEEEEECCCCCEEEEEEEECCCEEEEEECCCCEEEEE
KLSQSQNVLSNFGIIVPKKTVNEILKIVSHGSGNDLISISLSDRKICFEYKEYILISKVI
ECCCHHHHHHHCCEEEEHHHHHHHHHHHCCCCCCCEEEEEECCCEEEHHHHHHEEEEHHH
DGTFPDYNSIIPRSHDKNVLIESETLSNAIDRVSVVVFDKVKVIKLRFVSNKLILSAASS
CCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHEEEEEECCEEEEEEEEECCEEEEEECCC
DQGDAREELEVDYSGEDMTIGLNARYLMEALYCIKGKCLLSFYDAGTSVLIQEEENIDDF
CCCCCCHHEEEEECCCEEEEECCHHHHHHHHHHHCCEEEEEEECCCCEEEEEECCCCCCE
TYIIMPMRI
EEEEEEECC
>Mature Secondary Structure
MNFFVSKDKLLQALSYINSIVEKRHSIVVLSCVRIEARDQEIKLTSTDLDISIIASITAH
CCCEECHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCEEEEEECCCCEEEEEEEEEE
VNVNGVVVVSAQLLYDIVKKLPNDIEIKFFIGEDGRLTIKCGRTEFFLPVVDIEKFPIIE
EECCEEEEEEHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCEEEEEEEECCCCCCCCC
NNELQNKFTLQVSELVSLFGKTKFAISIEESRFNLNGLYVHTSNNILCCAATDGHRLSLT
CCCCCCEEEEEHHHHHHHHCCEEEEEEEECCCCCEEEEEEEECCCEEEEEECCCCEEEEE
KLSQSQNVLSNFGIIVPKKTVNEILKIVSHGSGNDLISISLSDRKICFEYKEYILISKVI
ECCCHHHHHHHCCEEEEHHHHHHHHHHHCCCCCCCEEEEEECCCEEEHHHHHHEEEEHHH
DGTFPDYNSIIPRSHDKNVLIESETLSNAIDRVSVVVFDKVKVIKLRFVSNKLILSAASS
CCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHEEEEEECCEEEEEEEEECCEEEEEECCC
DQGDAREELEVDYSGEDMTIGLNARYLMEALYCIKGKCLLSFYDAGTSVLIQEEENIDDF
CCCCCCHHEEEEECCCEEEEECCHHHHHHHHHHHCCEEEEEEECCCCEEEEEECCCCCCE
TYIIMPMRI
EEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]