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The map label for this gene is dagA [H]

Identifier: 88658607

GI number: 88658607

Start: 794797

End: 796179

Strand: Direct

Name: dagA [H]

Synonym: ECH_0781

Alternate gene names: 88658607

Gene position: 794797-796179 (Clockwise)

Preceding gene: 88658585

Following gene: 88658314

Centisome position: 67.57

GC content: 30.01

Gene sequence:

>1383_bases
ATGACAATCCTTGAATTTGCAGATTTTTTTCCACACATTTCTGGAGTATTAGAGTCTATTTTTTCTCTCACAATATTCAG
AATTCCACTAATTATTTTATGGGTAATATGTTTAGGAACATTCTGCACACTAAAATTTAAATTTATCAATATAAGACTTT
TTAAACACGCTTTATATGTTCTAATTAAGCCATATAACAAAAAAAACAGTAATACTATTTCTAATATCCAAGCATTTACT
ACAGCAATATCTGGAACTATAGGATTAGGAACAATTTCAGGAGTAATTATAGCAATTTCTACAGGAGGTCCAGGAGCAGT
ATTGTGGATGATAATAATAGGTATTTTAGGTATGTCAGTAAAATTTGCTGAAGTAACATTAGGATTCAAATATTACTTGT
TAGAAAAGAAAAAAAATATAGGAGGACCTTTTCAATATATCAAATGTGGATTAGCTGAATTAGGATTAGTTAAAACAGGA
AAAATCATCGCATACATATATGCTGTCATAATGATAATAGCTATGTCTTTTGGAGGAATATCATTTCAATCAAATCAAGC
TACTGTATTATTAAGCAACACCTTCCACAAAATAAACAGCAGTACTATTGTTATAACACTAACACTATTAAGTGGATTAG
TAATAATGGGAGGAATAACACGTATTGCAAATATTATGACAAAATTATCTCCCATAATGGTTATATTATATATAGTCGTA
TGTACAGCTGTTATTATTAGTCATTCAGATCAACTACTTTCTACGCTAAGCACAATGCTACACGGTATAATAGAAGGAAA
TAGTGTTACTGGAGGAGCAATAGGATCTTTTGTTGCAGGGGTTAGAAGATCAGTTTTCGCAAATGAAGCTGGAATTGGTA
CTTCTCCTATCATACATGCCTCAACAAATGATACTGAACCTGCAAGAGTAGGATGCATTGCAATGCTTGAACCATTTATT
GACACTGTACTTGTATCATTTATCACGGGAATAGTTGTTATACTAACTGGATCCTATAGCACTATAAACAGTAGTAATCA
TGTAATAATAATTAACAATATATTTTCCAACATTTGTCCAATTTGTGAATATATTATACTCCCTTTTATCATATTCTCCT
TCGCATTTTCATCAATTATCACATACTCTTATTACTGTGAAATATCTTGGAAACATATATTTGGAAATTCATGTCATGTT
GTATTATGTCGGACATTAATAATGTGTATCATTCTAATTAGTGGATTATCCAAAAACATAGTTCCTATATCTCAATTAGG
AGACCTGCTCTTTATATTTCTCACAATACCTAATGGACTGACAATTATCTTATTAAGTGGTACTATAAATCAAGAACTTA
AGAAATATTTATGCAAAAACTAA

Upstream 100 bases:

>100_bases
GTTGGAATGATGTATATATTATCAATTCCTATAAGTAGCATGGTATACATATACATTATGTGCAAAACTAAAGGTAAAAT
AATCTAGTACACAATTAACA

Downstream 100 bases:

>100_bases
TTGTATTACGAACATCTATTATAACAACCATTAAAATAACAAATTGAGTTTCTCACACAATAAAGTTCATATTTCATATC
AAAAAAACTAGCAATTCTAG

Product: sodium:alanine symporter family protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MTILEFADFFPHISGVLESIFSLTIFRIPLIILWVICLGTFCTLKFKFINIRLFKHALYVLIKPYNKKNSNTISNIQAFT
TAISGTIGLGTISGVIIAISTGGPGAVLWMIIIGILGMSVKFAEVTLGFKYYLLEKKKNIGGPFQYIKCGLAELGLVKTG
KIIAYIYAVIMIIAMSFGGISFQSNQATVLLSNTFHKINSSTIVITLTLLSGLVIMGGITRIANIMTKLSPIMVILYIVV
CTAVIISHSDQLLSTLSTMLHGIIEGNSVTGGAIGSFVAGVRRSVFANEAGIGTSPIIHASTNDTEPARVGCIAMLEPFI
DTVLVSFITGIVVILTGSYSTINSSNHVIIINNIFSNICPICEYIILPFIIFSFAFSSIITYSYYCEISWKHIFGNSCHV
VLCRTLIMCIILISGLSKNIVPISQLGDLLFIFLTIPNGLTIILLSGTINQELKKYLCKN

Sequences:

>Translated_460_residues
MTILEFADFFPHISGVLESIFSLTIFRIPLIILWVICLGTFCTLKFKFINIRLFKHALYVLIKPYNKKNSNTISNIQAFT
TAISGTIGLGTISGVIIAISTGGPGAVLWMIIIGILGMSVKFAEVTLGFKYYLLEKKKNIGGPFQYIKCGLAELGLVKTG
KIIAYIYAVIMIIAMSFGGISFQSNQATVLLSNTFHKINSSTIVITLTLLSGLVIMGGITRIANIMTKLSPIMVILYIVV
CTAVIISHSDQLLSTLSTMLHGIIEGNSVTGGAIGSFVAGVRRSVFANEAGIGTSPIIHASTNDTEPARVGCIAMLEPFI
DTVLVSFITGIVVILTGSYSTINSSNHVIIINNIFSNICPICEYIILPFIIFSFAFSSIITYSYYCEISWKHIFGNSCHV
VLCRTLIMCIILISGLSKNIVPISQLGDLLFIFLTIPNGLTIILLSGTINQELKKYLCKN
>Mature_459_residues
TILEFADFFPHISGVLESIFSLTIFRIPLIILWVICLGTFCTLKFKFINIRLFKHALYVLIKPYNKKNSNTISNIQAFTT
AISGTIGLGTISGVIIAISTGGPGAVLWMIIIGILGMSVKFAEVTLGFKYYLLEKKKNIGGPFQYIKCGLAELGLVKTGK
IIAYIYAVIMIIAMSFGGISFQSNQATVLLSNTFHKINSSTIVITLTLLSGLVIMGGITRIANIMTKLSPIMVILYIVVC
TAVIISHSDQLLSTLSTMLHGIIEGNSVTGGAIGSFVAGVRRSVFANEAGIGTSPIIHASTNDTEPARVGCIAMLEPFID
TVLVSFITGIVVILTGSYSTINSSNHVIIINNIFSNICPICEYIILPFIIFSFAFSSIITYSYYCEISWKHIFGNSCHVV
LCRTLIMCIILISGLSKNIVPISQLGDLLFIFLTIPNGLTIILLSGTINQELKKYLCKN

Specific function: Catalyzes the sodium-dependent uptake of extracellular D-alanine and glycine [H]

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=467, Percent_Identity=25.4817987152034, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 49992; Mature: 49860

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILEFADFFPHISGVLESIFSLTIFRIPLIILWVICLGTFCTLKFKFINIRLFKHALYV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHH
LIKPYNKKNSNTISNIQAFTTAISGTIGLGTISGVIIAISTGGPGAVLWMIIIGILGMSV
EEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCEEEEEECCCCHHHHHHHHHHHHCCCE
KFAEVTLGFKYYLLEKKKNIGGPFQYIKCGLAELGLVKTGKIIAYIYAVIMIIAMSFGGI
EEHHEEECHHEEEEEHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCC
SFQSNQATVLLSNTFHKINSSTIVITLTLLSGLVIMGGITRIANIMTKLSPIMVILYIVV
EEECCCEEEEEECCHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CTAVIISHSDQLLSTLSTMLHGIIEGNSVTGGAIGSFVAGVRRSVFANEAGIGTSPIIHA
HHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEC
STNDTEPARVGCIAMLEPFIDTVLVSFITGIVVILTGSYSTINSSNHVIIINNIFSNICP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEECCCCCEEEEHHHHHHHHH
ICEYIILPFIIFSFAFSSIITYSYYCEISWKHIFGNSCHVVLCRTLIMCIILISGLSKNI
HHHHHHHHHHHHHHHHHHHHHEEEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHCCCCCC
VPISQLGDLLFIFLTIPNGLTIILLSGTINQELKKYLCKN
CCHHHHHHHHEEEEECCCCCEEEEEECCHHHHHHHHHCCC
>Mature Secondary Structure 
TILEFADFFPHISGVLESIFSLTIFRIPLIILWVICLGTFCTLKFKFINIRLFKHALYV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHH
LIKPYNKKNSNTISNIQAFTTAISGTIGLGTISGVIIAISTGGPGAVLWMIIIGILGMSV
EEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCEEEEEECCCCHHHHHHHHHHHHCCCE
KFAEVTLGFKYYLLEKKKNIGGPFQYIKCGLAELGLVKTGKIIAYIYAVIMIIAMSFGGI
EEHHEEECHHEEEEEHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCC
SFQSNQATVLLSNTFHKINSSTIVITLTLLSGLVIMGGITRIANIMTKLSPIMVILYIVV
EEECCCEEEEEECCHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CTAVIISHSDQLLSTLSTMLHGIIEGNSVTGGAIGSFVAGVRRSVFANEAGIGTSPIIHA
HHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEC
STNDTEPARVGCIAMLEPFIDTVLVSFITGIVVILTGSYSTINSSNHVIIINNIFSNICP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEECCCCCEEEEHHHHHHHHH
ICEYIILPFIIFSFAFSSIITYSYYCEISWKHIFGNSCHVVLCRTLIMCIILISGLSKNI
HHHHHHHHHHHHHHHHHHHHHEEEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHCCCCCC
VPISQLGDLLFIFLTIPNGLTIILLSGTINQELKKYLCKN
CCHHHHHHHHEEEEECCCCCEEEEEECCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1447975 [H]