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The map label for this gene is topA [H]

Identifier: 88658490

GI number: 88658490

Start: 753014

End: 753796

Strand: Direct

Name: topA [H]

Synonym: ECH_0746

Alternate gene names: 88658490

Gene position: 753014-753796 (Clockwise)

Preceding gene: 88658007

Following gene: 88658100

Centisome position: 64.02

GC content: 28.86

Gene sequence:

>783_bases
TTGGATTTAATCTCTAATGGGAAGATTAATTGGAAGGAAGTACTACATCAATTCTGGTTTCTGTTTATTGATAATGTTAA
TTCAGTAAAAAAACTAGAATTTACTGAAGTATTAAAAGTTATTAGTTGTGAGATGGAAAATTATATTTTTTCTTCTGATA
AATCTGAATCTAGTAGAGTATGTCCTGTATGTAATGATGGAACATTAGAGCTTAATATTAGTAAGAATGGTATTTTTTTA
GGATGTTCTAAATATCCAGAGTGTAAGTATACAAAAAATGTTGGAAGTGGTATTGTTGATGATACAGACAATACACAATA
TCCTAAAATATTAGGTATTGATGCTGCAACAGGACAAGAAATATATCTTAAAAAAGGTCCGTATGGTCTCTATTTACAGA
TTGGTAATGATAAAAGTGGTAAACGTGCAGCTATTCCAAAAAATATAGATGCTAATTTATTAGATTTGCAAATGGCAGAA
AAAATATTAAGTTTACCTATAAGCTTAGGTACTCATCCGGATACTGGTCAAGAAATTAAGTTAGGACTTGGTAAGTTTGG
TGCATACATATTGTATGGTGGTAAGTATTTTTCAGTAAAAAATGAGCATAATTTTTTAGATATTAGCATTGAAAACGCTA
TTAATATTATTGCGAATAATTCTACAAGTGGGGTTATAAAGTCTCTGGGTATCACTGAAGATAATAAAGAAGTATTCTTA
ACACTAATAATGATATTACAAAAAATAGATCATGCGTTATATGATTACTTAGGGGCTAAGTAG

Upstream 100 bases:

>100_bases
ACAATGGGTACCTAGTTATAACAACTACAAATAAAAATACTTCATTAATTTTTTTAGTCGCTATGTAGAATATGATTTTA
CTGCTGACCTTGAAGAAAAG

Downstream 100 bases:

>100_bases
TTCAGTGGCATAGTAGGAGAATCATAATTTCTCAGTCGCGGGTTCAAATCCCTCCTTAGCCACTTAATTCATTAGCATTA
GAGAGTCCTTGGTATTCTAT

Product: topoisomerase DNA-binding C4 zinc finger domain-containing protein

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MDLISNGKINWKEVLHQFWFLFIDNVNSVKKLEFTEVLKVISCEMENYIFSSDKSESSRVCPVCNDGTLELNISKNGIFL
GCSKYPECKYTKNVGSGIVDDTDNTQYPKILGIDAATGQEIYLKKGPYGLYLQIGNDKSGKRAAIPKNIDANLLDLQMAE
KILSLPISLGTHPDTGQEIKLGLGKFGAYILYGGKYFSVKNEHNFLDISIENAINIIANNSTSGVIKSLGITEDNKEVFL
TLIMILQKIDHALYDYLGAK

Sequences:

>Translated_260_residues
MDLISNGKINWKEVLHQFWFLFIDNVNSVKKLEFTEVLKVISCEMENYIFSSDKSESSRVCPVCNDGTLELNISKNGIFL
GCSKYPECKYTKNVGSGIVDDTDNTQYPKILGIDAATGQEIYLKKGPYGLYLQIGNDKSGKRAAIPKNIDANLLDLQMAE
KILSLPISLGTHPDTGQEIKLGLGKFGAYILYGGKYFSVKNEHNFLDISIENAINIIANNSTSGVIKSLGITEDNKEVFL
TLIMILQKIDHALYDYLGAK
>Mature_260_residues
MDLISNGKINWKEVLHQFWFLFIDNVNSVKKLEFTEVLKVISCEMENYIFSSDKSESSRVCPVCNDGTLELNISKNGIFL
GCSKYPECKYTKNVGSGIVDDTDNTQYPKILGIDAATGQEIYLKKGPYGLYLQIGNDKSGKRAAIPKNIDANLLDLQMAE
KILSLPISLGTHPDTGQEIKLGLGKFGAYILYGGKYFSVKNEHNFLDISIENAINIIANNSTSGVIKSLGITEDNKEVFL
TLIMILQKIDHALYDYLGAK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG1754

COG function: function code R; Uncharacterized C-terminal domain of topoisomerase IA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 28894; Mature: 28894

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLISNGKINWKEVLHQFWFLFIDNVNSVKKLEFTEVLKVISCEMENYIFSSDKSESSRV
CCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCC
CPVCNDGTLELNISKNGIFLGCSKYPECKYTKNVGSGIVDDTDNTQYPKILGIDAATGQE
CEEECCCEEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCE
IYLKKGPYGLYLQIGNDKSGKRAAIPKNIDANLLDLQMAEKILSLPISLGTHPDTGQEIK
EEEEECCEEEEEEECCCCCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEE
LGLGKFGAYILYGGKYFSVKNEHNFLDISIENAINIIANNSTSGVIKSLGITEDNKEVFL
EECCCCCEEEEECCEEEEECCCCCEEEEEHHCEEEEEECCCCCHHHHCCCCCCCCHHHHH
TLIMILQKIDHALYDYLGAK
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLISNGKINWKEVLHQFWFLFIDNVNSVKKLEFTEVLKVISCEMENYIFSSDKSESSRV
CCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCC
CPVCNDGTLELNISKNGIFLGCSKYPECKYTKNVGSGIVDDTDNTQYPKILGIDAATGQE
CEEECCCEEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCE
IYLKKGPYGLYLQIGNDKSGKRAAIPKNIDANLLDLQMAEKILSLPISLGTHPDTGQEIK
EEEEECCEEEEEEECCCCCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEE
LGLGKFGAYILYGGKYFSVKNEHNFLDISIENAINIIANNSTSGVIKSLGITEDNKEVFL
EECCCCCEEEEECCEEEEECCCCCEEEEEHHCEEEEEECCCCCHHHHCCCCCCCCHHHHH
TLIMILQKIDHALYDYLGAK
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]