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The map label for this gene is ribF [H]

Identifier: 88658244

GI number: 88658244

Start: 1088747

End: 1089700

Strand: Reverse

Name: ribF [H]

Synonym: ECH_1061

Alternate gene names: 88658244

Gene position: 1089700-1088747 (Counterclockwise)

Preceding gene: 88658386

Following gene: 88657909

Centisome position: 92.64

GC content: 28.41

Gene sequence:

>954_bases
ATGAAAATAATATATGGTTATCCTAACCAAAAAGGTAATGTAAATAGTGTTCTTGCTTTTGGGAATTTTGATGGAATACA
TTTAGGACATCAGTCTATAATTAATGCAATAAAAAATATTTCAGTTAGAGAAAAGATAACATCTGTGGTTATTACATTTT
CTCCACATCCTGCTGAGTATTTGCGTAATAAACAAAATTTTTTGTTATTAGATCTTGAGCAAAAGATAGAATTGTTGCAA
AGCTATGGTATAGATTATCTATATATTATTGATTTTAATGAAAGTTTTGCACAGCTTTCTCCGGATGCTTTTATAAAGGA
TGTATTAGTAAATAGTTGTAGAATAAAATATATTGTAGTAGGACATAGTTGTTTTTTTGGACATAAATGTTTAGGTAACA
TAGATTTGTTATATTCTTATGCAAATATTTATGATTATGAAATTGTAAGAATAGATCCTATTTTTATAAATGATAAATTA
TGCTCTTCTTCATTGATTAGAGAGTATTTGTCTGAAGGAAAAGTAGACTTAGCAAGTAAGATTTTGGGTAGACCGTATCA
AATTAATGGCAAAGTGATAAAAGGATTGGCTAGGGGCCGCGTTATTGGTTTTCCTACAGTTAATGTTGATATTAAACATA
TATTAGTGCCTAGAGTTGGGGTGTATTCTGCATGTATAAAAATAGATGATAATAATGTGTGGTTAAATGGGATAGTTAAT
ATTGGATTTAGACCTACATTTAATGATTTAAGTTTTCCTATATTGGAAATGCATATTTTTGATTTTGATAGTGATATATA
TAATCAGCATGTAGCTATTCAGTTATTGGACTTCATACGTCCTGAACGTAAATTTAGTAGTATTGAGCAGTTAAAACAGC
AGATTAACGAGGATATTATTCAAGTAAAGAAAAGTTTAAAGTATATTGGGAGCAAAGATGACGAAGTAGTGTAA

Upstream 100 bases:

>100_bases
TTTTCACTTTTATCACTATAATGTATCCTATGATTAACTGAATTTCTGGATATCATAGTAATAGCAAAAAGTAAATATAG
GAATTTTATAAAATTTTATT

Downstream 100 bases:

>100_bases
ATGTTTAGCTATAGTACAGTAGATTAATAGAGATATTGCTTAAATGGAAAAAAGTTGATGCAAGATATGTATAAGTATAT
GCTTATAAGTCAACTTACTG

Product: riboflavin biosynthesis protein RibF

Products: NA

Alternate protein names: Riboflavin kinase; Flavokinase; FMN adenylyltransferase; FAD pyrophosphorylase; FAD synthase [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MKIIYGYPNQKGNVNSVLAFGNFDGIHLGHQSIINAIKNISVREKITSVVITFSPHPAEYLRNKQNFLLLDLEQKIELLQ
SYGIDYLYIIDFNESFAQLSPDAFIKDVLVNSCRIKYIVVGHSCFFGHKCLGNIDLLYSYANIYDYEIVRIDPIFINDKL
CSSSLIREYLSEGKVDLASKILGRPYQINGKVIKGLARGRVIGFPTVNVDIKHILVPRVGVYSACIKIDDNNVWLNGIVN
IGFRPTFNDLSFPILEMHIFDFDSDIYNQHVAIQLLDFIRPERKFSSIEQLKQQINEDIIQVKKSLKYIGSKDDEVV

Sequences:

>Translated_317_residues
MKIIYGYPNQKGNVNSVLAFGNFDGIHLGHQSIINAIKNISVREKITSVVITFSPHPAEYLRNKQNFLLLDLEQKIELLQ
SYGIDYLYIIDFNESFAQLSPDAFIKDVLVNSCRIKYIVVGHSCFFGHKCLGNIDLLYSYANIYDYEIVRIDPIFINDKL
CSSSLIREYLSEGKVDLASKILGRPYQINGKVIKGLARGRVIGFPTVNVDIKHILVPRVGVYSACIKIDDNNVWLNGIVN
IGFRPTFNDLSFPILEMHIFDFDSDIYNQHVAIQLLDFIRPERKFSSIEQLKQQINEDIIQVKKSLKYIGSKDDEVV
>Mature_317_residues
MKIIYGYPNQKGNVNSVLAFGNFDGIHLGHQSIINAIKNISVREKITSVVITFSPHPAEYLRNKQNFLLLDLEQKIELLQ
SYGIDYLYIIDFNESFAQLSPDAFIKDVLVNSCRIKYIVVGHSCFFGHKCLGNIDLLYSYANIYDYEIVRIDPIFINDKL
CSSSLIREYLSEGKVDLASKILGRPYQINGKVIKGLARGRVIGFPTVNVDIKHILVPRVGVYSACIKIDDNNVWLNGIVN
IGFRPTFNDLSFPILEMHIFDFDSDIYNQHVAIQLLDFIRPERKFSSIEQLKQQINEDIIQVKKSLKYIGSKDDEVV

Specific function: Unknown

COG id: COG0196

COG function: function code H; FAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribF family [H]

Homologues:

Organism=Escherichia coli, GI1786208, Length=307, Percent_Identity=32.8990228013029, Blast_Score=181, Evalue=6e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015864
- InterPro:   IPR015865
- InterPro:   IPR002606
- InterPro:   IPR014729 [H]

Pfam domain/function: PF06574 FAD_syn; PF01687 Flavokinase [H]

EC number: =2.7.1.26; =2.7.7.2 [H]

Molecular weight: Translated: 36217; Mature: 36217

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIYGYPNQKGNVNSVLAFGNFDGIHLGHQSIINAIKNISVREKITSVVITFSPHPAEY
CEEEEECCCCCCCCCEEEEECCCCCEECCHHHHHHHHHCCHHHHHEEEEEEEECCCCHHH
LRNKQNFLLLDLEQKIELLQSYGIDYLYIIDFNESFAQLSPDAFIKDVLVNSCRIKYIVV
HCCCCCEEEEEHHHHHHHHHHCCCCEEEEEECCCHHHHCCHHHHHHHHHHCCCEEEEEEE
GHSCFFGHKCLGNIDLLYSYANIYDYEIVRIDPIFINDKLCSSSLIREYLSEGKVDLASK
ECCEEECCHHHCCHHHEEEHHCEEEEEEEEEEEEEECCHHHHHHHHHHHHHCCCHHHHHH
ILGRPYQINGKVIKGLARGRVIGFPTVNVDIKHILVPRVGVYSACIKIDDNNVWLNGIVN
HCCCCEEECCHHHHHHHCCCEEECCEECCEEEEEECCCCCCEEEEEEEECCCEEEEEEEE
IGFRPTFNDLSFPILEMHIFDFDSDIYNQHVAIQLLDFIRPERKFSSIEQLKQQINEDII
CCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
QVKKSLKYIGSKDDEVV
HHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKIIYGYPNQKGNVNSVLAFGNFDGIHLGHQSIINAIKNISVREKITSVVITFSPHPAEY
CEEEEECCCCCCCCCEEEEECCCCCEECCHHHHHHHHHCCHHHHHEEEEEEEECCCCHHH
LRNKQNFLLLDLEQKIELLQSYGIDYLYIIDFNESFAQLSPDAFIKDVLVNSCRIKYIVV
HCCCCCEEEEEHHHHHHHHHHCCCCEEEEEECCCHHHHCCHHHHHHHHHHCCCEEEEEEE
GHSCFFGHKCLGNIDLLYSYANIYDYEIVRIDPIFINDKLCSSSLIREYLSEGKVDLASK
ECCEEECCHHHCCHHHEEEHHCEEEEEEEEEEEEEECCHHHHHHHHHHHHHCCCHHHHHH
ILGRPYQINGKVIKGLARGRVIGFPTVNVDIKHILVPRVGVYSACIKIDDNNVWLNGIVN
HCCCCEEECCHHHHHHHCCCEEECCEECCEEEEEECCCCCCEEEEEEEECCCEEEEEEEE
IGFRPTFNDLSFPILEMHIFDFDSDIYNQHVAIQLLDFIRPERKFSSIEQLKQQINEDII
CCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
QVKKSLKYIGSKDDEVV
HHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]