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The map label for this gene is miaB

Identifier: 88658201

GI number: 88658201

Start: 854812

End: 856140

Strand: Direct

Name: miaB

Synonym: ECH_0837

Alternate gene names: 88658201

Gene position: 854812-856140 (Clockwise)

Preceding gene: 88657603

Following gene: 88657604

Centisome position: 72.67

GC content: 31.45

Gene sequence:

>1329_bases
ATGCAAGGTTTATACATCAAGTCATATGGCTGTCAAATGAACGTTTATGACTCTCTAATCATGGAAAATATAATAAAACC
CCTTGGATTCACTGTAGTGAATGAGCCATCTGAAGCAAATATTGTAATATTAAATACGTGTCATATTAGAGAGAAAGCTT
CTGAAAAGCTATATTCAGAGCTAGGTAAAATGAGAAAAATACAAGAAACAAAAGACTTAACTATAGTAGTTGCTGGATGT
GTAGCACAAGCTGAAGGAGAACAAATATTTGCAAGAGCTCCATTTGTTGATATAGTGGTAGGTCCTCAAAGTATACATAC
ACTTCCCGAACTTATTATAAAAGCTCGTAGGATGAAAAAACAAGTTATCAATATTGATTTCCCTATAATTTCAAAATTTG
ATGCAATACCAGTAGAAGAGTACACAAAAAATCAAGAAGTTTCTGCTTTTATTTCAGTACAAGAAGGATGTAATAAGTTT
TGTACATTCTGTGTAGTACCTTATACACGTGGTGAAGAATACTCAAGAACTGTAGAAGCAATATTTAACGAAGCACTTGT
TTTATCAGATTCTGGAGTAAAGGAAATAACCCTAATTGGACAGAATGTAAATGCATACCACGGAACATATAAAGGATGTG
AATGGGATTTAGGAAAATTAATTCAGTATCTTGCAAAAATACCAAATATAGAACGTATAAGATATACCACATCACATCCA
CGTGACATGCACCAATCTCTATATGAAGCACATAGATCTGAGACAAAACTAATGCCATTCGTTCACCTACCAATACAATC
AGGCTCAGATAGAATACTAAAAAAAATGAATAGAAAACATACTGCTGAAGAATATATAGATATTATCAATAATTTAAGAA
AACAACGCCCTGACATTGCTTTTTCATCTGATTTCATAGTTGGATTTCCAGGAGAAACAGAAGAAGATTTTGAACATACA
ATGAAATTAGTACAAGAAGTAAACTTTTCACAGGCATATAGTTTTAAATATAGTCCAAGACCAGGGACACCTAGTGCAGA
ATACCCAAATCAAATACCAGAAGAAATAAAATCTCAGCGTATTTTTAGATTACAAGAATTATTAAGAGAACAGCAGCTTG
CTTTTAATAGAAACATGATTGGTCAAACTTGTTCTGTATTGTTTAATAATAAGAAAGGAAAATTTGATAATCAAATAATA
GGTAAAACTGAATATATGCAATCCTGCTACATTAACACAGATAATACCAATCAATTTTATAATAGTATATTACCTATTAA
AATAATAGACGCTTATCAAAATAGCGTAACCGGCATAGTTGTTAATTGA

Upstream 100 bases:

>100_bases
ACTTTTTATAAATATTCAATAACTTAACATAATAAAAATTATTGTATTAGTTCATTAATCATTATATTCTTAACAGAGTA
AAAGCAGTTATTTAGAGTTA

Downstream 100 bases:

>100_bases
TAAAATGCAATGCTTTTGCCGTAAATATAATTTTAACATCTAAAACTTTCTAAGATACATTCATAATAGTTTCTCAACAA
TGCTTATCTATTAAGTAATA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MQGLYIKSYGCQMNVYDSLIMENIIKPLGFTVVNEPSEANIVILNTCHIREKASEKLYSELGKMRKIQETKDLTIVVAGC
VAQAEGEQIFARAPFVDIVVGPQSIHTLPELIIKARRMKKQVINIDFPIISKFDAIPVEEYTKNQEVSAFISVQEGCNKF
CTFCVVPYTRGEEYSRTVEAIFNEALVLSDSGVKEITLIGQNVNAYHGTYKGCEWDLGKLIQYLAKIPNIERIRYTTSHP
RDMHQSLYEAHRSETKLMPFVHLPIQSGSDRILKKMNRKHTAEEYIDIINNLRKQRPDIAFSSDFIVGFPGETEEDFEHT
MKLVQEVNFSQAYSFKYSPRPGTPSAEYPNQIPEEIKSQRIFRLQELLREQQLAFNRNMIGQTCSVLFNNKKGKFDNQII
GKTEYMQSCYINTDNTNQFYNSILPIKIIDAYQNSVTGIVVN

Sequences:

>Translated_442_residues
MQGLYIKSYGCQMNVYDSLIMENIIKPLGFTVVNEPSEANIVILNTCHIREKASEKLYSELGKMRKIQETKDLTIVVAGC
VAQAEGEQIFARAPFVDIVVGPQSIHTLPELIIKARRMKKQVINIDFPIISKFDAIPVEEYTKNQEVSAFISVQEGCNKF
CTFCVVPYTRGEEYSRTVEAIFNEALVLSDSGVKEITLIGQNVNAYHGTYKGCEWDLGKLIQYLAKIPNIERIRYTTSHP
RDMHQSLYEAHRSETKLMPFVHLPIQSGSDRILKKMNRKHTAEEYIDIINNLRKQRPDIAFSSDFIVGFPGETEEDFEHT
MKLVQEVNFSQAYSFKYSPRPGTPSAEYPNQIPEEIKSQRIFRLQELLREQQLAFNRNMIGQTCSVLFNNKKGKFDNQII
GKTEYMQSCYINTDNTNQFYNSILPIKIIDAYQNSVTGIVVN
>Mature_442_residues
MQGLYIKSYGCQMNVYDSLIMENIIKPLGFTVVNEPSEANIVILNTCHIREKASEKLYSELGKMRKIQETKDLTIVVAGC
VAQAEGEQIFARAPFVDIVVGPQSIHTLPELIIKARRMKKQVINIDFPIISKFDAIPVEEYTKNQEVSAFISVQEGCNKF
CTFCVVPYTRGEEYSRTVEAIFNEALVLSDSGVKEITLIGQNVNAYHGTYKGCEWDLGKLIQYLAKIPNIERIRYTTSHP
RDMHQSLYEAHRSETKLMPFVHLPIQSGSDRILKKMNRKHTAEEYIDIINNLRKQRPDIAFSSDFIVGFPGETEEDFEHT
MKLVQEVNFSQAYSFKYSPRPGTPSAEYPNQIPEEIKSQRIFRLQELLREQQLAFNRNMIGQTCSVLFNNKKGKFDNQII
GKTEYMQSCYINTDNTNQFYNSILPIKIIDAYQNSVTGIVVN

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=430, Percent_Identity=32.093023255814, Blast_Score=254, Evalue=8e-68,
Organism=Homo sapiens, GI28872782, Length=430, Percent_Identity=32.093023255814, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI93277076, Length=384, Percent_Identity=27.8645833333333, Blast_Score=162, Evalue=5e-40,
Organism=Escherichia coli, GI1786882, Length=441, Percent_Identity=46.9387755102041, Blast_Score=414, Evalue=1e-117,
Organism=Escherichia coli, GI1787057, Length=444, Percent_Identity=29.2792792792793, Blast_Score=183, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17553146, Length=414, Percent_Identity=32.8502415458937, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI71996771, Length=318, Percent_Identity=28.3018867924528, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21356207, Length=431, Percent_Identity=31.7865429234339, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI19922432, Length=409, Percent_Identity=27.1393643031785, Blast_Score=142, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_EHRCR (Q2GG00)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507633.1
- ProteinModelPortal:   Q2GG00
- SMR:   Q2GG00
- STRING:   Q2GG00
- GeneID:   3927997
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0837
- TIGR:   ECH_0837
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- PhylomeDB:   Q2GG00
- ProtClustDB:   PRK14325
- BioCyc:   ECHA205920:ECH_0837-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50640; Mature: 50640

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGLYIKSYGCQMNVYDSLIMENIIKPLGFTVVNEPSEANIVILNTCHIREKASEKLYSE
CCCCEEECCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEECCCHHHHHHHHHHH
LGKMRKIQETKDLTIVVAGCVAQAEGEQIFARAPFVDIVVGPQSIHTLPELIIKARRMKK
HHHHHHHHHCCCEEEEEEEHHHHCCCCEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHH
QVINIDFPIISKFDAIPVEEYTKNQEVSAFISVQEGCNKFCTFCVVPYTRGEEYSRTVEA
HHHCCCCHHHCCCCCCCHHHHCCCCHHHHEEEHHHHHHHHHHEEEEECCCCCHHHHHHHH
IFNEALVLSDSGVKEITLIGQNVNAYHGTYKGCEWDLGKLIQYLAKIPNIERIRYTTSHP
HHCCEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCC
RDMHQSLYEAHRSETKLMPFVHLPIQSGSDRILKKMNRKHTAEEYIDIINNLRKQRPDIA
HHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCE
FSSDFIVGFPGETEEDFEHTMKLVQEVNFSQAYSFKYSPRPGTPSAEYPNQIPEEIKSQR
ECCCEEEECCCCCHHHHHHHHHHHHHCCCHHHEEEEECCCCCCCCCCCCHHHHHHHHHHH
IFRLQELLREQQLAFNRNMIGQTCSVLFNNKKGKFDNQIIGKTEYMQSCYINTDNTNQFY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCHHHHHHHHEECCCCCHHHH
NSILPIKIIDAYQNSVTGIVVN
HCCCCEEEEECCCCCCCEEEEC
>Mature Secondary Structure
MQGLYIKSYGCQMNVYDSLIMENIIKPLGFTVVNEPSEANIVILNTCHIREKASEKLYSE
CCCCEEECCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEECCCHHHHHHHHHHH
LGKMRKIQETKDLTIVVAGCVAQAEGEQIFARAPFVDIVVGPQSIHTLPELIIKARRMKK
HHHHHHHHHCCCEEEEEEEHHHHCCCCEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHH
QVINIDFPIISKFDAIPVEEYTKNQEVSAFISVQEGCNKFCTFCVVPYTRGEEYSRTVEA
HHHCCCCHHHCCCCCCCHHHHCCCCHHHHEEEHHHHHHHHHHEEEEECCCCCHHHHHHHH
IFNEALVLSDSGVKEITLIGQNVNAYHGTYKGCEWDLGKLIQYLAKIPNIERIRYTTSHP
HHCCEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCC
RDMHQSLYEAHRSETKLMPFVHLPIQSGSDRILKKMNRKHTAEEYIDIINNLRKQRPDIA
HHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCE
FSSDFIVGFPGETEEDFEHTMKLVQEVNFSQAYSFKYSPRPGTPSAEYPNQIPEEIKSQR
ECCCEEEECCCCCHHHHHHHHHHHHHCCCHHHEEEEECCCCCCCCCCCCHHHHHHHHHHH
IFRLQELLREQQLAFNRNMIGQTCSVLFNNKKGKFDNQIIGKTEYMQSCYINTDNTNQFY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCHHHHHHHHEECCCCCHHHH
NSILPIKIIDAYQNSVTGIVVN
HCCCCEEEEECCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA