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The map label for this gene is uvrD [H]

Identifier: 88657941

GI number: 88657941

Start: 881517

End: 883436

Strand: Direct

Name: uvrD [H]

Synonym: ECH_0860

Alternate gene names: 88657941

Gene position: 881517-883436 (Clockwise)

Preceding gene: 88657927

Following gene: 88658334

Centisome position: 74.94

GC content: 29.95

Gene sequence:

>1920_bases
ATGGAAGATTATATAACTTCACTTAATGAAGACCAGAGGGAAGCTGTCATTAATATAAATGGTCCTATTTTAATATTAGC
CGGAGCAGGTACAGGTAAAACTAGAACTATTACTTCAAGAATAGCATACATCATTAATAACAATTTTGCATTACCTAGCC
AAATTTTAGCAGTAACATTTACTAATAAAGCAGCAAACGAAATGCTATCTAGAATTAGTGAATTAACACCTGTATATAAT
ATCTGGTTAGGAACTTTTCATGCAATAGCAGCAAAAATCTTAAGACAGCATGCTGAAGTAGTTGGATTAAAAAACGATTT
TACAATCATTAACACAGACGACCAACTACAAGTAATAAAAAATATTGTCAATGACATGCATCCCGAATATGCATCAGATG
CCCATAAAATTATCTTAAATAAAATACAAAGATGGAAAGATAGAGGACTTATACCAAGCAATATTACTGAAACAGAGTTA
TATAAACCTATTAATACAGTAGCATTGAGTACATATCACATTTATCAAGAAAGATTAAAATTTTTAAACTGCGTTGATTT
TGGAGACTTACTACTATACAACATTCAAATATTCACTACACAACATAATGTCTTATCCCATTACCAAGAACAATTTAAAT
ACATAATGGTTGATGAATATCAGGACATAAATACAATTCAATATTTATGGTTAAGATTATTAGCTCAAAAATACAAAAAT
CTATGCTGTGTTGGAGATGATGACCAATCAATATATAGTTGGCGGGGAGCAGAAGTAGGAAATATTTTAAAATTTTCTGA
TGATTTTCCAAATGCAAAAATTGTTCGATTAGAATGTAACTACAGATCAACATCTAATATATTAGACGTTGCCACAGCGA
TAATAAATAACAATAAATCAAGGTTAGGAAAAAAGCTATGGACAACAAACGAGAAAGGACAAAAAGTTAATTTAATGAAA
TTTTGGGATAGTAAAGCAGAAGCACAGTATATATGTGAATATATTAAAAATTTACATGACTACCAATTTAACGAGACAGC
TATTTTAGTAAGGGCAGGATTTCAAACAAGAATATTCGAGGAATACTTCATTAAATATAGCATACCATATAAGATAATTG
GTGGTACAAGATTCTACGATAGACAAGAAATTCGTGATATCATCGCCTATTTAAAAATTACAGTTAACCCTAATAATGAC
ATCGCTTTTGAAAGAATAATTAATAAACCTAAACGACATATAGGAGCATCAACTCTTAATAAAATATACTTATGTGCTAA
ACAAAATAATACATATTTTTTGAACGCAGCAAAAATTCTAATTTCAAATAATCAACTACCTGACAAAGCATCCCGATCTT
TAAATAACTTATTAAATAAGATTGACTCTTGGAAAGAATCTTTATCATCTGATTCAATATCAAACACTGTTAAAATGATA
GCTTACGACTCAGGATATGTAGACATGTTAGAAAACGAAGGTGAAAATGGTTTAGCAAGAATAGAAAACATAAAAGAACT
TTTTTCTGCATTATTAAGTTTTAATGATGTAAATGAATTTTTAGAACACATCAGCCTTGTAACAGAAACAGACTCATTAA
ATTACAATGACAACTACACATATATTATGACATTACATGCAGCAAAAGGATTAGAATTCCCTATAGTTTTTCTACCTGGA
TGGGAAGAAGGAGTATTTCCACATGAAAAATCTTTACAAGACAGAACAGGAGAAGCACTAGAAGAAGAGAGAAGACTCGC
CTATGTTGGGATTACACGAGCACGTGAACAACTATTTATATCATGCGTAGCAGTAAGAGATATTAACAACTGGCGCCAGC
AAATGAAAATATCAAGATTTATAAAGGAATTACCTGCAGAACATGTACAAGTTATAAAAAATATTTCTAGCTATTATTAA

Upstream 100 bases:

>100_bases
ATTAACAAACCGTTCTCAAAAATATAATATTAAGATCTTACTTTGACTGTACCAGAAAAAAAATATAATATACTCCAGAT
AATGATGAATTTATTGCATT

Downstream 100 bases:

>100_bases
TTATTAAGTTTTGTGAATCGGTTAATGCTAATAACAGCATTATGTAACAGTATAGAATTCTTTACCATTTCAACACATAG
GGTATGATGTGCAACACTAA

Product: UvrD/Rep family helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 639; Mature: 639

Protein sequence:

>639_residues
MEDYITSLNEDQREAVININGPILILAGAGTGKTRTITSRIAYIINNNFALPSQILAVTFTNKAANEMLSRISELTPVYN
IWLGTFHAIAAKILRQHAEVVGLKNDFTIINTDDQLQVIKNIVNDMHPEYASDAHKIILNKIQRWKDRGLIPSNITETEL
YKPINTVALSTYHIYQERLKFLNCVDFGDLLLYNIQIFTTQHNVLSHYQEQFKYIMVDEYQDINTIQYLWLRLLAQKYKN
LCCVGDDDQSIYSWRGAEVGNILKFSDDFPNAKIVRLECNYRSTSNILDVATAIINNNKSRLGKKLWTTNEKGQKVNLMK
FWDSKAEAQYICEYIKNLHDYQFNETAILVRAGFQTRIFEEYFIKYSIPYKIIGGTRFYDRQEIRDIIAYLKITVNPNND
IAFERIINKPKRHIGASTLNKIYLCAKQNNTYFLNAAKILISNNQLPDKASRSLNNLLNKIDSWKESLSSDSISNTVKMI
AYDSGYVDMLENEGENGLARIENIKELFSALLSFNDVNEFLEHISLVTETDSLNYNDNYTYIMTLHAAKGLEFPIVFLPG
WEEGVFPHEKSLQDRTGEALEEERRLAYVGITRAREQLFISCVAVRDINNWRQQMKISRFIKELPAEHVQVIKNISSYY

Sequences:

>Translated_639_residues
MEDYITSLNEDQREAVININGPILILAGAGTGKTRTITSRIAYIINNNFALPSQILAVTFTNKAANEMLSRISELTPVYN
IWLGTFHAIAAKILRQHAEVVGLKNDFTIINTDDQLQVIKNIVNDMHPEYASDAHKIILNKIQRWKDRGLIPSNITETEL
YKPINTVALSTYHIYQERLKFLNCVDFGDLLLYNIQIFTTQHNVLSHYQEQFKYIMVDEYQDINTIQYLWLRLLAQKYKN
LCCVGDDDQSIYSWRGAEVGNILKFSDDFPNAKIVRLECNYRSTSNILDVATAIINNNKSRLGKKLWTTNEKGQKVNLMK
FWDSKAEAQYICEYIKNLHDYQFNETAILVRAGFQTRIFEEYFIKYSIPYKIIGGTRFYDRQEIRDIIAYLKITVNPNND
IAFERIINKPKRHIGASTLNKIYLCAKQNNTYFLNAAKILISNNQLPDKASRSLNNLLNKIDSWKESLSSDSISNTVKMI
AYDSGYVDMLENEGENGLARIENIKELFSALLSFNDVNEFLEHISLVTETDSLNYNDNYTYIMTLHAAKGLEFPIVFLPG
WEEGVFPHEKSLQDRTGEALEEERRLAYVGITRAREQLFISCVAVRDINNWRQQMKISRFIKELPAEHVQVIKNISSYY
>Mature_639_residues
MEDYITSLNEDQREAVININGPILILAGAGTGKTRTITSRIAYIINNNFALPSQILAVTFTNKAANEMLSRISELTPVYN
IWLGTFHAIAAKILRQHAEVVGLKNDFTIINTDDQLQVIKNIVNDMHPEYASDAHKIILNKIQRWKDRGLIPSNITETEL
YKPINTVALSTYHIYQERLKFLNCVDFGDLLLYNIQIFTTQHNVLSHYQEQFKYIMVDEYQDINTIQYLWLRLLAQKYKN
LCCVGDDDQSIYSWRGAEVGNILKFSDDFPNAKIVRLECNYRSTSNILDVATAIINNNKSRLGKKLWTTNEKGQKVNLMK
FWDSKAEAQYICEYIKNLHDYQFNETAILVRAGFQTRIFEEYFIKYSIPYKIIGGTRFYDRQEIRDIIAYLKITVNPNND
IAFERIINKPKRHIGASTLNKIYLCAKQNNTYFLNAAKILISNNQLPDKASRSLNNLLNKIDSWKESLSSDSISNTVKMI
AYDSGYVDMLENEGENGLARIENIKELFSALLSFNDVNEFLEHISLVTETDSLNYNDNYTYIMTLHAAKGLEFPIVFLPG
WEEGVFPHEKSLQDRTGEALEEERRLAYVGITRAREQLFISCVAVRDINNWRQQMKISRFIKELPAEHVQVIKNISSYY

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=643, Percent_Identity=40.746500777605, Blast_Score=482, Evalue=1e-137,
Organism=Escherichia coli, GI48994965, Length=638, Percent_Identity=33.8557993730408, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1787196, Length=338, Percent_Identity=28.1065088757396, Blast_Score=109, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6322369, Length=746, Percent_Identity=28.4182305630027, Blast_Score=208, Evalue=2e-54,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 73860; Mature: 73860

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDYITSLNEDQREAVININGPILILAGAGTGKTRTITSRIAYIINNNFALPSQILAVTF
CCCHHHCCCCCCCCEEEEECCCEEEEECCCCCCCHHHHHHHHHHEECCCCCCCCEEEEEE
TNKAANEMLSRISELTPVYNIWLGTFHAIAAKILRQHAEVVGLKNDFTIINTDDQLQVIK
CCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCHHHHHHH
NIVNDMHPEYASDAHKIILNKIQRWKDRGLIPSNITETELYKPINTVALSTYHIYQERLK
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHH
FLNCVDFGDLLLYNIQIFTTQHNVLSHYQEQFKYIMVDEYQDINTIQYLWLRLLAQKYKN
HHHHCCCCCEEEEEEEEEEECHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHHHHHHCC
LCCVGDDDQSIYSWRGAEVGNILKFSDDFPNAKIVRLECNYRSTSNILDVATAIINNNKS
EEEECCCCCHHHEECCCCCCCEEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCHH
RLGKKLWTTNEKGQKVNLMKFWDSKAEAQYICEYIKNLHDYQFNETAILVRAGFQTRIFE
HHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHH
EYFIKYSIPYKIIGGTRFYDRQEIRDIIAYLKITVNPNNDIAFERIINKPKRHIGASTLN
HHHHEEECCEEEECCCCCCCHHHHHHHHHHEEEEECCCCCHHHHHHHCCHHHHCCHHHHC
KIYLCAKQNNTYFLNAAKILISNNQLPDKASRSLNNLLNKIDSWKESLSSDSISNTVKMI
EEEEEEECCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCEEEEE
AYDSGYVDMLENEGENGLARIENIKELFSALLSFNDVNEFLEHISLVTETDSLNYNDNYT
EECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEE
YIMTLHAAKGLEFPIVFLPGWEEGVFPHEKSLQDRTGEALEEERRLAYVGITRAREQLFI
EEEEEEECCCCCCCEEEECCCCCCCCCCHHHHHHHCCHHHHHCCCEEEEEHHHHHHHHHH
SCVAVRDINNWRQQMKISRFIKELPAEHVQVIKNISSYY
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MEDYITSLNEDQREAVININGPILILAGAGTGKTRTITSRIAYIINNNFALPSQILAVTF
CCCHHHCCCCCCCCEEEEECCCEEEEECCCCCCCHHHHHHHHHHEECCCCCCCCEEEEEE
TNKAANEMLSRISELTPVYNIWLGTFHAIAAKILRQHAEVVGLKNDFTIINTDDQLQVIK
CCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCHHHHHHH
NIVNDMHPEYASDAHKIILNKIQRWKDRGLIPSNITETELYKPINTVALSTYHIYQERLK
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHH
FLNCVDFGDLLLYNIQIFTTQHNVLSHYQEQFKYIMVDEYQDINTIQYLWLRLLAQKYKN
HHHHCCCCCEEEEEEEEEEECHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHHHHHHCC
LCCVGDDDQSIYSWRGAEVGNILKFSDDFPNAKIVRLECNYRSTSNILDVATAIINNNKS
EEEECCCCCHHHEECCCCCCCEEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCHH
RLGKKLWTTNEKGQKVNLMKFWDSKAEAQYICEYIKNLHDYQFNETAILVRAGFQTRIFE
HHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHH
EYFIKYSIPYKIIGGTRFYDRQEIRDIIAYLKITVNPNNDIAFERIINKPKRHIGASTLN
HHHHEEECCEEEECCCCCCCHHHHHHHHHHEEEEECCCCCHHHHHHHCCHHHHCCHHHHC
KIYLCAKQNNTYFLNAAKILISNNQLPDKASRSLNNLLNKIDSWKESLSSDSISNTVKMI
EEEEEEECCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCEEEEE
AYDSGYVDMLENEGENGLARIENIKELFSALLSFNDVNEFLEHISLVTETDSLNYNDNYT
EECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEE
YIMTLHAAKGLEFPIVFLPGWEEGVFPHEKSLQDRTGEALEEERRLAYVGITRAREQLFI
EEEEEEECCCCCCCEEEECCCCCCCCCCHHHHHHHCCHHHHHCCCEEEEEHHHHHHHHHH
SCVAVRDINNWRQQMKISRFIKELPAEHVQVIKNISSYY
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA