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The map label for this gene is purB [H]

Identifier: 88657927

GI number: 88657927

Start: 872422

End: 873720

Strand: Direct

Name: purB [H]

Synonym: ECH_0852

Alternate gene names: 88657927

Gene position: 872422-873720 (Clockwise)

Preceding gene: 88658526

Following gene: 88657941

Centisome position: 74.17

GC content: 31.02

Gene sequence:

>1299_bases
ATGATCAAACGTTACAGTAGACCTGAAATGTCATCAATTTGGGAAGACAATAATAAGTTCAAAATATGGATGGACATAGA
ATACTACGCATGCGAAGCACAAGCAAAATTAGGTGTTATACCTGATCACATACTCAATAATTTAAAAAACAAAATTGAAC
ATTTTGATATTAACCGCATAAATGAAATCGAGTCAGTAGTAAAACACGACGTAATAGCATTTTTAACATATATATCAGAA
TCTACCAATGCAGATATAAGATACTTACACTACGGTATGACAAGCTCTGATGTATTAGATACTTGCTTATCCATACAATT
AAAGCAATCTTGTAATTTATTATTAGATAACATAGAAATCATATTAAACATTTTAAAACAAAAATCTATAGAAACAAAAT
ATATATTATGCATGGGACGTAGTCATGGCGTACATGCAGAACCAGTAACATTTGGATTAAAACTTGCAAGATTTTACGCA
GAATTTAAGCGTAATTACAACAGATTAAAAATGGCTCAAGATGAAATATCAGTATGTAAGATTTCTGGAGCTATGGGTAA
CTTTGCCAACATTGATCCATTTGTTGAAAATTATGTAGCAAATGCATTAAATCTTAGACCAGAATCTATATCATCACAAG
TAATACCAAGAGATCGCCATGCTATGTTTTTTGCAACTTTAGGAGTTATTGCATCTTCCATAGAAAACATAGCAATAGAA
ATTCGTCACCTTCAAAAAACTGAAACACTAGAAGTCACAGAATATTTTTCAGCTGGACAGAAAGGAAGTTCTGCAATGCC
TCACAAGAAAAACCCCATATTAAGTGAAAATCTTACAGGCCTTGCCAGAATGATACGAAGTTACGTAATTCCTGCTCTAG
AAAATGTTGCTTTATGGCACGAAAGAGATATATCACATTCCTCTGTAGAAAGATGTATTGCACCAGATGCTTGTATTGTT
ACAGATTTTGCATTAGCAAGGTTAGCAAATTTGCTCAATAACATGATCATATATCCTAATAATATGGATAAGAATCTAGC
ACAATCAAAAGGTTTAGTATTCTCTCAACGGGTACTTCTTGAATTAGTAAAATATGGGTTAAAAAGAGAAACAGCTTATC
AAATAATTCAAGAACGGGCATTAAAAACATGGAATAACGATAGCCATTTTCTAGATGAACTAAAAAAAGATCCACAAGTA
TTAAAATGTATAAGTAACGAAAGTTTAGAATCTTTATTTAACTTTGATTATTATACACAACATATAGACACAATATTCGA
TAAGGTTTTTAAAGAATAA

Upstream 100 bases:

>100_bases
ATATTACCACCATCATACTTCAAGTGTTATTAGATATTATATATCAAATAACATCTAAACATTTACAATAAAAATATATT
AAAACCTCGGAGACAAACAA

Downstream 100 bases:

>100_bases
ATAATTAATTAAGTTCAGTTTTTAATGTTAATTAATATATAAAGTCATTATGAAGCTACTAAGTTATTAATTTTTAAACT
AAATATTAAAAAATATAAGA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MIKRYSRPEMSSIWEDNNKFKIWMDIEYYACEAQAKLGVIPDHILNNLKNKIEHFDINRINEIESVVKHDVIAFLTYISE
STNADIRYLHYGMTSSDVLDTCLSIQLKQSCNLLLDNIEIILNILKQKSIETKYILCMGRSHGVHAEPVTFGLKLARFYA
EFKRNYNRLKMAQDEISVCKISGAMGNFANIDPFVENYVANALNLRPESISSQVIPRDRHAMFFATLGVIASSIENIAIE
IRHLQKTETLEVTEYFSAGQKGSSAMPHKKNPILSENLTGLARMIRSYVIPALENVALWHERDISHSSVERCIAPDACIV
TDFALARLANLLNNMIIYPNNMDKNLAQSKGLVFSQRVLLELVKYGLKRETAYQIIQERALKTWNNDSHFLDELKKDPQV
LKCISNESLESLFNFDYYTQHIDTIFDKVFKE

Sequences:

>Translated_432_residues
MIKRYSRPEMSSIWEDNNKFKIWMDIEYYACEAQAKLGVIPDHILNNLKNKIEHFDINRINEIESVVKHDVIAFLTYISE
STNADIRYLHYGMTSSDVLDTCLSIQLKQSCNLLLDNIEIILNILKQKSIETKYILCMGRSHGVHAEPVTFGLKLARFYA
EFKRNYNRLKMAQDEISVCKISGAMGNFANIDPFVENYVANALNLRPESISSQVIPRDRHAMFFATLGVIASSIENIAIE
IRHLQKTETLEVTEYFSAGQKGSSAMPHKKNPILSENLTGLARMIRSYVIPALENVALWHERDISHSSVERCIAPDACIV
TDFALARLANLLNNMIIYPNNMDKNLAQSKGLVFSQRVLLELVKYGLKRETAYQIIQERALKTWNNDSHFLDELKKDPQV
LKCISNESLESLFNFDYYTQHIDTIFDKVFKE
>Mature_432_residues
MIKRYSRPEMSSIWEDNNKFKIWMDIEYYACEAQAKLGVIPDHILNNLKNKIEHFDINRINEIESVVKHDVIAFLTYISE
STNADIRYLHYGMTSSDVLDTCLSIQLKQSCNLLLDNIEIILNILKQKSIETKYILCMGRSHGVHAEPVTFGLKLARFYA
EFKRNYNRLKMAQDEISVCKISGAMGNFANIDPFVENYVANALNLRPESISSQVIPRDRHAMFFATLGVIASSIENIAIE
IRHLQKTETLEVTEYFSAGQKGSSAMPHKKNPILSENLTGLARMIRSYVIPALENVALWHERDISHSSVERCIAPDACIV
TDFALARLANLLNNMIIYPNNMDKNLAQSKGLVFSQRVLLELVKYGLKRETAYQIIQERALKTWNNDSHFLDELKKDPQV
LKCISNESLESLFNFDYYTQHIDTIFDKVFKE

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=390, Percent_Identity=25.6410256410256, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI183227688, Length=383, Percent_Identity=25.3263707571802, Blast_Score=139, Evalue=7e-33,
Organism=Escherichia coli, GI1787376, Length=284, Percent_Identity=27.112676056338, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17508577, Length=394, Percent_Identity=25.3807106598985, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32564234, Length=340, Percent_Identity=26.4705882352941, Blast_Score=98, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6323391, Length=393, Percent_Identity=24.1730279898219, Blast_Score=127, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24647570, Length=451, Percent_Identity=23.059866962306, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49633; Mature: 49633

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRYSRPEMSSIWEDNNKFKIWMDIEYYACEAQAKLGVIPDHILNNLKNKIEHFDINRI
CCCCCCCCCHHHHHCCCCEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHH
NEIESVVKHDVIAFLTYISESTNADIRYLHYGMTSSDVLDTCLSIQLKQSCNLLLDNIEI
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ILNILKQKSIETKYILCMGRSHGVHAEPVTFGLKLARFYAEFKRNYNRLKMAQDEISVCK
HHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHEE
ISGAMGNFANIDPFVENYVANALNLRPESISSQVIPRDRHAMFFATLGVIASSIENIAIE
ECCCCCCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IRHLQKTETLEVTEYFSAGQKGSSAMPHKKNPILSENLTGLARMIRSYVIPALENVALWH
HHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERDISHSSVERCIAPDACIVTDFALARLANLLNNMIIYPNNMDKNLAQSKGLVFSQRVLL
HCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHCCCHHHHHHHH
ELVKYGLKRETAYQIIQERALKTWNNDSHFLDELKKDPQVLKCISNESLESLFNFDYYTQ
HHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHCCHHHHHHHCCHHHHH
HIDTIFDKVFKE
HHHHHHHHHHCC
>Mature Secondary Structure
MIKRYSRPEMSSIWEDNNKFKIWMDIEYYACEAQAKLGVIPDHILNNLKNKIEHFDINRI
CCCCCCCCCHHHHHCCCCEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHH
NEIESVVKHDVIAFLTYISESTNADIRYLHYGMTSSDVLDTCLSIQLKQSCNLLLDNIEI
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ILNILKQKSIETKYILCMGRSHGVHAEPVTFGLKLARFYAEFKRNYNRLKMAQDEISVCK
HHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHEE
ISGAMGNFANIDPFVENYVANALNLRPESISSQVIPRDRHAMFFATLGVIASSIENIAIE
ECCCCCCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IRHLQKTETLEVTEYFSAGQKGSSAMPHKKNPILSENLTGLARMIRSYVIPALENVALWH
HHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERDISHSSVERCIAPDACIVTDFALARLANLLNNMIIYPNNMDKNLAQSKGLVFSQRVLL
HCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHCCCHHHHHHHH
ELVKYGLKRETAYQIIQERALKTWNNDSHFLDELKKDPQVLKCISNESLESLFNFDYYTQ
HHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHCCHHHHHHHCCHHHHH
HIDTIFDKVFKE
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]