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The map label for this gene is yfhF [C]

Identifier: 88657844

GI number: 88657844

Start: 638332

End: 638775

Strand: Direct

Name: yfhF [C]

Synonym: ECH_0631

Alternate gene names: 88657844

Gene position: 638332-638775 (Clockwise)

Preceding gene: 88658479

Following gene: 88658431

Centisome position: 54.27

GC content: 29.05

Gene sequence:

>444_bases
ATGATGAATAGTGAAACTCCTACATTTTATAATAGTGAAGACAGTGAAGATAATTCATGCAGTTCACAGATCAAGTATCA
CATAAATAGCAAAATAACAAACAAATCTCCCATTATTATTACAGAAAGTGCTATAAAAAAAATAAGAGAGCTTATTAAAC
AAAAAGAAGGTGCTGTTATTGGTATCAGGATTATGGTGAAACAAAAAGGATGTTTTGGTCTCAAGTATAATATTGAATAT
GCACATGATATCAAGCTATTAGATGTAGAAATTAAAGTAGAATATCAAAATGAGAATTTTACTATATTAATTGACCCCAA
AGCAATGATGTTTATCAATGGAACAGAAATGGACCATGTAGAAAACAAAATATCTTCAGGTTTTACATTTAATAATCCTA
ATGAGAAAGGAAGATGTGGATGTGGGGAGAGTTTCTATGTATAA

Upstream 100 bases:

>100_bases
GTAAAAGCTGCCATAAAGGATTATAACATGAAGCAAGCAACCAAAAAAGACAAGCTTTCATCTTCAAATGAATAGATATC
TATTAAAGTAAGGTTTACAT

Downstream 100 bases:

>100_bases
AGACTATTTTTCATTATTCAAGTTAAAACCACAATTCTCTATAAACATGCAAACATTAGAACAGAATTACATAGCATTAC
AAAGCAATTACCATCCAGAT

Product: iron-sulfur cluster assembly accessory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MMNSETPTFYNSEDSEDNSCSSQIKYHINSKITNKSPIIITESAIKKIRELIKQKEGAVIGIRIMVKQKGCFGLKYNIEY
AHDIKLLDVEIKVEYQNENFTILIDPKAMMFINGTEMDHVENKISSGFTFNNPNEKGRCGCGESFYV

Sequences:

>Translated_147_residues
MMNSETPTFYNSEDSEDNSCSSQIKYHINSKITNKSPIIITESAIKKIRELIKQKEGAVIGIRIMVKQKGCFGLKYNIEY
AHDIKLLDVEIKVEYQNENFTILIDPKAMMFINGTEMDHVENKISSGFTFNNPNEKGRCGCGESFYV
>Mature_147_residues
MMNSETPTFYNSEDSEDNSCSSQIKYHINSKITNKSPIIITESAIKKIRELIKQKEGAVIGIRIMVKQKGCFGLKYNIEY
AHDIKLLDVEIKVEYQNENFTILIDPKAMMFINGTEMDHVENKISSGFTFNNPNEKGRCGCGESFYV

Specific function: Unknown

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family [H]

Homologues:

Organism=Homo sapiens, GI52426768, Length=112, Percent_Identity=42.8571428571429, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1788877, Length=110, Percent_Identity=31.8181818181818, Blast_Score=80, Evalue=6e-17,
Organism=Escherichia coli, GI1787974, Length=110, Percent_Identity=34.5454545454545, Blast_Score=75, Evalue=1e-15,
Organism=Escherichia coli, GI1786351, Length=111, Percent_Identity=27.027027027027, Blast_Score=68, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI25146411, Length=114, Percent_Identity=42.1052631578947, Blast_Score=100, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI193209025, Length=113, Percent_Identity=42.4778761061947, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323001, Length=131, Percent_Identity=37.4045801526718, Blast_Score=91, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24642263, Length=114, Percent_Identity=42.1052631578947, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870 [H]

Pfam domain/function: PF01521 Fe-S_biosyn [H]

EC number: NA

Molecular weight: Translated: 16773; Mature: 16773

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS01152 HESB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNSETPTFYNSEDSEDNSCSSQIKYHINSKITNKSPIIITESAIKKIRELIKQKEGAVI
CCCCCCCEEECCCCCCCCCCCCEEEEEECCEECCCCCEEEEHHHHHHHHHHHHHCCCCEE
GIRIMVKQKGCFGLKYNIEYAHDIKLLDVEIKVEYQNENFTILIDPKAMMFINGTEMDHV
EEEEEEEECCCEEEEEEEEEECCEEEEEEEEEEEECCCCEEEEECCCEEEEEECCCCHHH
ENKISSGFTFNNPNEKGRCGCGESFYV
HHHHHCCCEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMNSETPTFYNSEDSEDNSCSSQIKYHINSKITNKSPIIITESAIKKIRELIKQKEGAVI
CCCCCCCEEECCCCCCCCCCCCEEEEEECCEECCCCCEEEEHHHHHHHHHHHHHCCCCEE
GIRIMVKQKGCFGLKYNIEYAHDIKLLDVEIKVEYQNENFTILIDPKAMMFINGTEMDHV
EEEEEEEECCCEEEEEEEEEECCEEEEEEEEEEEECCCCEEEEECCCEEEEEECCCCHHH
ENKISSGFTFNNPNEKGRCGCGESFYV
HHHHHCCCEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]