Click here to switch to the map view.

The map label for this gene is dnaA

Identifier: 88608587

GI number: 88608587

Start: 220315

End: 221739

Strand: Direct

Name: dnaA

Synonym: NSE_0259

Alternate gene names: 88608587

Gene position: 220315-221739 (Clockwise)

Preceding gene: 88608456

Following gene: 88608511

Centisome position: 25.65

GC content: 41.12

Gene sequence:

>1425_bases
GTGTTGCTAGCGAAGCAACATGACAGGCGCATTGATGGTATCGGTCCAACAGAATGGGATAGGGTAACGAGAAAGTTACG
CAAATTTTACGGCGAAAAGATTTATGGAAGCTGGTTAAAGTTCATGTCTTTTAAGGAGATGAGATGTGGTCAGGTTTTTC
TTTCTGTTCCAACGGGGTTCATAAAGGAGTGGATCACCGTGAACTATTTGGGTAACATTCTTGGGTTTTTGCGTAATGAA
AATCCGGCAATTACCTCGATAGAAATAGGTGTTAATAAAGAGCATGATAGGGCTCTAGAATCAGACCGTGAAGAGCTGGT
TGCACCCTTTGTGAAATCCAAAATCAGTAGTGAAAAGGGTCGATTTGATAGAGGAAGTTTTTCTGAAAATTTAAGTAGCA
GCATAGATCGAAGTAGGACATTTGATAGTTTTGTTGTTGGTAAGTCAAATGAGCTTGCATTTACGGCTTCCAAGCGTGTC
GCAGAAGCTGTTGAGTTACCAATTTCAGGAAGTAACCCTCTATTTCTGTATGGTGGTGTGGGGCTTGGAAAAACACATCT
CATGCACGCAATTGTTTGGCACATAAAAGCAAACTATGCAGACCGTAGAGTTATTTACCTCTCAGCAGAAAAATTCATGC
ACAAGTACATTACCGCATTGAAGAACAAGGACATGATGTCATTCAAGCAAATTTTTAGGTCTGTGGATGTCTTGATGGTT
GATGATGTGCAATTTATTAGTGGCAAGGAAAGCACTCAGGAGGAGTTTTTTCACACGTTTAACGCTCTTATAGAGCAAAA
CAAGCAGCTTGTTATTTCGGCTGATAGATCTCCCAGTGATCTACAAGGGGTTGAGGAACGTATACAGTCGCGTTTATCTT
GGGGGCTCGTTGCAGACATTAATCAGACGACATTTGAACTGCGTGTTGGTATTTTGGAGTCTAAATTGGAGCAAATGAAG
TCTGATGTTGTCGTTCCAAAGAAAGTAGTAGAGTTCATGGCAAGAAATATAGTGTCGAACGTGCGTGAGTTAGAAGGTGC
ACTTAACAAAGTTATAGCTCACTCAGAGATTACCGGGAGTGTTGTCACAGTTGATTCTGCTAAAGATTTGTTGGCTGATA
TACTGCGGACAAATAGTGTTGCTGTCACTTTAGAAAATATTCTTGCTAAGGTATCGGAATACTTTGGTATTAAGATTTCA
GATCTTAAGTCGCAAAGAAGAGTCAAGGATGTTGCCTATGCGAGGCAAATGTGCATGTTCCTCGCTAAGACTCTTACGCA
GAGAAGTCTCATCGATATAGGGAAGTTCCTCGGGGGTAGAGATCACGCTACGGTGATACATTCTGTGAGAAAAATCGAGA
TTTTGATTAAGAATGACGCAAGAGTCGCTGAAGATGTCAGAATACTCACTAAGAGGTTGACATGA

Upstream 100 bases:

>100_bases
TGGGGTGGTGAGCGACCTTTTAGATGGAGGTTGTGAGGATGGAATGTATCACGATAGACAGGGCGTTGATGGTGTTGCGG
ATTCATATTCCTCAGACGCG

Downstream 100 bases:

>100_bases
TCACTGAAACTGTCACTTCTTCATTGATGTAATAGTATAAGATTTGTTATCGGGGTTCAGTTTTGTGATGAGTTCTCATG
TGGCTGTTGTTGGTGCGACA

Product: chromosomal replication initiator protein DnaA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MLLAKQHDRRIDGIGPTEWDRVTRKLRKFYGEKIYGSWLKFMSFKEMRCGQVFLSVPTGFIKEWITVNYLGNILGFLRNE
NPAITSIEIGVNKEHDRALESDREELVAPFVKSKISSEKGRFDRGSFSENLSSSIDRSRTFDSFVVGKSNELAFTASKRV
AEAVELPISGSNPLFLYGGVGLGKTHLMHAIVWHIKANYADRRVIYLSAEKFMHKYITALKNKDMMSFKQIFRSVDVLMV
DDVQFISGKESTQEEFFHTFNALIEQNKQLVISADRSPSDLQGVEERIQSRLSWGLVADINQTTFELRVGILESKLEQMK
SDVVVPKKVVEFMARNIVSNVRELEGALNKVIAHSEITGSVVTVDSAKDLLADILRTNSVAVTLENILAKVSEYFGIKIS
DLKSQRRVKDVAYARQMCMFLAKTLTQRSLIDIGKFLGGRDHATVIHSVRKIEILIKNDARVAEDVRILTKRLT

Sequences:

>Translated_474_residues
MLLAKQHDRRIDGIGPTEWDRVTRKLRKFYGEKIYGSWLKFMSFKEMRCGQVFLSVPTGFIKEWITVNYLGNILGFLRNE
NPAITSIEIGVNKEHDRALESDREELVAPFVKSKISSEKGRFDRGSFSENLSSSIDRSRTFDSFVVGKSNELAFTASKRV
AEAVELPISGSNPLFLYGGVGLGKTHLMHAIVWHIKANYADRRVIYLSAEKFMHKYITALKNKDMMSFKQIFRSVDVLMV
DDVQFISGKESTQEEFFHTFNALIEQNKQLVISADRSPSDLQGVEERIQSRLSWGLVADINQTTFELRVGILESKLEQMK
SDVVVPKKVVEFMARNIVSNVRELEGALNKVIAHSEITGSVVTVDSAKDLLADILRTNSVAVTLENILAKVSEYFGIKIS
DLKSQRRVKDVAYARQMCMFLAKTLTQRSLIDIGKFLGGRDHATVIHSVRKIEILIKNDARVAEDVRILTKRLT
>Mature_474_residues
MLLAKQHDRRIDGIGPTEWDRVTRKLRKFYGEKIYGSWLKFMSFKEMRCGQVFLSVPTGFIKEWITVNYLGNILGFLRNE
NPAITSIEIGVNKEHDRALESDREELVAPFVKSKISSEKGRFDRGSFSENLSSSIDRSRTFDSFVVGKSNELAFTASKRV
AEAVELPISGSNPLFLYGGVGLGKTHLMHAIVWHIKANYADRRVIYLSAEKFMHKYITALKNKDMMSFKQIFRSVDVLMV
DDVQFISGKESTQEEFFHTFNALIEQNKQLVISADRSPSDLQGVEERIQSRLSWGLVADINQTTFELRVGILESKLEQMK
SDVVVPKKVVEFMARNIVSNVRELEGALNKVIAHSEITGSVVTVDSAKDLLADILRTNSVAVTLENILAKVSEYFGIKIS
DLKSQRRVKDVAYARQMCMFLAKTLTQRSLIDIGKFLGGRDHATVIHSVRKIEILIKNDARVAEDVRILTKRLT

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=468, Percent_Identity=38.6752136752137, Blast_Score=337, Evalue=7e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 53751; Mature: 53751

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLAKQHDRRIDGIGPTEWDRVTRKLRKFYGEKIYGSWLKFMSFKEMRCGQVFLSVPTGF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHEEECCHHH
IKEWITVNYLGNILGFLRNENPAITSIEIGVNKEHDRALESDREELVAPFVKSKISSEKG
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHCCC
RFDRGSFSENLSSSIDRSRTFDSFVVGKSNELAFTASKRVAEAVELPISGSNPLFLYGGV
CCCCCCCHHHHHHHHHHHHCHHHHEECCCCCEEEHHHHHHHHHHHCCCCCCCCEEEEECC
GLGKTHLMHAIVWHIKANYADRRVIYLSAEKFMHKYITALKNKDMMSFKQIFRSVDVLMV
CCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEE
DDVQFISGKESTQEEFFHTFNALIEQNKQLVISADRSPSDLQGVEERIQSRLSWGLVADI
CCCHHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEECC
NQTTFELRVGILESKLEQMKSDVVVPKKVVEFMARNIVSNVRELEGALNKVIAHSEITGS
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VVTVDSAKDLLADILRTNSVAVTLENILAKVSEYFGIKISDLKSQRRVKDVAYARQMCMF
EEEECCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHH
LAKTLTQRSLIDIGKFLGGRDHATVIHSVRKIEILIKNDARVAEDVRILTKRLT
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MLLAKQHDRRIDGIGPTEWDRVTRKLRKFYGEKIYGSWLKFMSFKEMRCGQVFLSVPTGF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHEEECCHHH
IKEWITVNYLGNILGFLRNENPAITSIEIGVNKEHDRALESDREELVAPFVKSKISSEKG
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHCCC
RFDRGSFSENLSSSIDRSRTFDSFVVGKSNELAFTASKRVAEAVELPISGSNPLFLYGGV
CCCCCCCHHHHHHHHHHHHCHHHHEECCCCCEEEHHHHHHHHHHHCCCCCCCCEEEEECC
GLGKTHLMHAIVWHIKANYADRRVIYLSAEKFMHKYITALKNKDMMSFKQIFRSVDVLMV
CCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEE
DDVQFISGKESTQEEFFHTFNALIEQNKQLVISADRSPSDLQGVEERIQSRLSWGLVADI
CCCHHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEECC
NQTTFELRVGILESKLEQMKSDVVVPKKVVEFMARNIVSNVRELEGALNKVIAHSEITGS
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VVTVDSAKDLLADILRTNSVAVTLENILAKVSEYFGIKISDLKSQRRVKDVAYARQMCMF
EEEECCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHH
LAKTLTQRSLIDIGKFLGGRDHATVIHSVRKIEILIKNDARVAEDVRILTKRLT
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA