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The map label for this gene is tpiA

Identifier: 88608298

GI number: 88608298

Start: 215641

End: 216366

Strand: Direct

Name: tpiA

Synonym: NSE_0254

Alternate gene names: 88608298

Gene position: 215641-216366 (Clockwise)

Preceding gene: 88608097

Following gene: 88607981

Centisome position: 25.1

GC content: 45.73

Gene sequence:

>726_bases
ATGGAAACTTGTGTGCAGCGCTTCGTGGTTGCAAATTGGAAGATGAATGGCAGTCAGCAATTGTGTGATGTTTTTGTGAA
TCGCTTTTCTGCTTTTGCTTTCAACCCTGAGGTCGAGATTGTAATAGCACCTCCGGCTGTCTACTTAGGACAGATGAGAG
CTGCTGTAATGTCTTCTGATTTGCCCTTAGCTCTGGGAGCACAGGATTGCGCTTCAGGTGATAATACTCCTAGCACTGGT
GACATAAGTTCTAGGATGTTTAAAGAGGTTGGTGCGAGGTATGTTCTTGTAGGACATTCCGAGAGGAGGACTTTGCACTG
TGAATCGAGTAAAGTGGTGGGTATGAAGGCCAATGCTGGATTACGCAGTCGCCTTATACCGATCATTTGTGTCGGACAGA
ATAACAGACCTATAAATAGAGAGAGTCTGACTAAGCAATGTAGGGATTCAATTCCAATGAATAGCCACGACTTCATAGTG
GCCTACGAGCCGGTCTATGCCATAGGAACAGGTATCGTGCCTTCGAACGAGGAGATTGAGGAGGCAGTCTGTTTAATCAA
GGATGCTGTTGGAGATGCAGTACCAGTGCTTTATGGTGGTTCTGTTTCTCCAGTTAATTGCTCAGAGATTTTTGCTGTGA
AGAACCTTGGTGGAGTGCTTGTAGGTGGTGCGAGCTTAGATCCGGATAGCTTCATCAAGATCTGTGAGGTTGCAAATCGG
TTTTGA

Upstream 100 bases:

>100_bases
AAGTATCAGTTAATTAGCAACTTCGGAGTTTTCCGGCAAAGGCTTTTTTTTATCTGTTGAAATTGATATCATGGATTCCC
GCAAGGATATATTGGAGAGT

Downstream 100 bases:

>100_bases
TCCTAGAGTCTTGACCCCTCTTTGAGTTGGGTTTGAAGCGGTTCTATGCTAGGCTATCCCGTGCTGGTGGATTTTTTGTC
GAACTTTTTATGGAGAAGTT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
METCVQRFVVANWKMNGSQQLCDVFVNRFSAFAFNPEVEIVIAPPAVYLGQMRAAVMSSDLPLALGAQDCASGDNTPSTG
DISSRMFKEVGARYVLVGHSERRTLHCESSKVVGMKANAGLRSRLIPIICVGQNNRPINRESLTKQCRDSIPMNSHDFIV
AYEPVYAIGTGIVPSNEEIEEAVCLIKDAVGDAVPVLYGGSVSPVNCSEIFAVKNLGGVLVGGASLDPDSFIKICEVANR
F

Sequences:

>Translated_241_residues
METCVQRFVVANWKMNGSQQLCDVFVNRFSAFAFNPEVEIVIAPPAVYLGQMRAAVMSSDLPLALGAQDCASGDNTPSTG
DISSRMFKEVGARYVLVGHSERRTLHCESSKVVGMKANAGLRSRLIPIICVGQNNRPINRESLTKQCRDSIPMNSHDFIV
AYEPVYAIGTGIVPSNEEIEEAVCLIKDAVGDAVPVLYGGSVSPVNCSEIFAVKNLGGVLVGGASLDPDSFIKICEVANR
F
>Mature_241_residues
METCVQRFVVANWKMNGSQQLCDVFVNRFSAFAFNPEVEIVIAPPAVYLGQMRAAVMSSDLPLALGAQDCASGDNTPSTG
DISSRMFKEVGARYVLVGHSERRTLHCESSKVVGMKANAGLRSRLIPIICVGQNNRPINRESLTKQCRDSIPMNSHDFIV
AYEPVYAIGTGIVPSNEEIEEAVCLIKDAVGDAVPVLYGGSVSPVNCSEIFAVKNLGGVLVGGASLDPDSFIKICEVANR
F

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI226529917, Length=242, Percent_Identity=34.7107438016529, Blast_Score=133, Evalue=1e-31,
Organism=Homo sapiens, GI4507645, Length=242, Percent_Identity=34.7107438016529, Blast_Score=133, Evalue=1e-31,
Organism=Escherichia coli, GI1790353, Length=248, Percent_Identity=32.258064516129, Blast_Score=130, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17536593, Length=243, Percent_Identity=34.9794238683128, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6320255, Length=246, Percent_Identity=31.3008130081301, Blast_Score=112, Evalue=6e-26,
Organism=Drosophila melanogaster, GI28572008, Length=251, Percent_Identity=33.0677290836653, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI28572006, Length=251, Percent_Identity=33.0677290836653, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI28572004, Length=251, Percent_Identity=33.0677290836653, Blast_Score=125, Evalue=3e-29,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00121 TIM [H]

EC number: =5.3.1.1 [H]

Molecular weight: Translated: 25932; Mature: 25932

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00171 TIM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METCVQRFVVANWKMNGSQQLCDVFVNRFSAFAFNPEVEIVIAPPAVYLGQMRAAVMSSD
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEECCCEEEEEECCHHHHHHHHHHHHCCC
LPLALGAQDCASGDNTPSTGDISSRMFKEVGARYVLVGHSERRTLHCESSKVVGMKANAG
CCEEECCHHHCCCCCCCCCCCHHHHHHHHHCCEEEEEECCCCCEEEECCCEEEEEECCCC
LRSRLIPIICVGQNNRPINRESLTKQCRDSIPMNSHDFIVAYEPVYAIGTGIVPSNEEIE
CCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCEEEECCCCCCCHHHH
EAVCLIKDAVGDAVPVLYGGSVSPVNCSEIFAVKNLGGVLVGGASLDPDSFIKICEVANR
HHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHC
F
C
>Mature Secondary Structure
METCVQRFVVANWKMNGSQQLCDVFVNRFSAFAFNPEVEIVIAPPAVYLGQMRAAVMSSD
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEECCCEEEEEECCHHHHHHHHHHHHCCC
LPLALGAQDCASGDNTPSTGDISSRMFKEVGARYVLVGHSERRTLHCESSKVVGMKANAG
CCEEECCHHHCCCCCCCCCCCHHHHHHHHHCCEEEEEECCCCCEEEECCCEEEEEECCCC
LRSRLIPIICVGQNNRPINRESLTKQCRDSIPMNSHDFIVAYEPVYAIGTGIVPSNEEIE
CCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCEEEECCCCCCCHHHH
EAVCLIKDAVGDAVPVLYGGSVSPVNCSEIFAVKNLGGVLVGGASLDPDSFIKICEVANR
HHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHC
F
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA