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The map label for this gene is thiL

Identifier: 88608097

GI number: 88608097

Start: 214520

End: 215461

Strand: Direct

Name: thiL

Synonym: NSE_0253

Alternate gene names: 88608097

Gene position: 214520-215461 (Clockwise)

Preceding gene: 88608484

Following gene: 88608298

Centisome position: 24.97

GC content: 42.68

Gene sequence:

>942_bases
GTGGGTTCGAGTCCCACTTTCGCTATTTCCTTCTTTATGGACGAGCATCGAATTCTCGCTGAGATTATTTCTGGGATTGC
AGAGCCTCTTTTTCTTCTGGACGATGCATTTGTGCTCGACGATTTTGTTATTACGAAAGATCTCTTGGTAGAGGATATCC
ATTTCTTCCCCTTCGCGGAACCGTATAAACTTGCGAAGAAAGCCTTAAGAGTTAACTTGTCTGACCTTGCTGCGATGGGT
GCTGAACCAGTCGGTTATTTTCTCGGACTTGTCTTAGGTGAAAAACATAAAAACTCAACATGGTTGAATAGATTTGCCGA
TGGTCTTCTTGATGATCAACGCTGCTATGGAATTAAACTTTTTGGTGGTGACACAGTTATTCATTCTGGGCCGCTTCTCA
TAAGTGTTACGATGATCGGGAAAAAAGCAGAGCGTGTTTTAACTCGTTCTGGTGCGGAAATTGGGGATGAGCTCTGTGTT
AGCGGGCCAATAGGTGACTCGCATCTGGGATTACTTTCGTATCAAGGCGAATTGCAAGAAAATTCATACTTTCGCAGCAA
GTACGATTTGCCGGAGCCATGCCTTGATGTGAGTAGGGATATAATCTCTAGCGTGACTTCCTGTATTGATATTTCTGATG
GTTTATTGAGTGAAGCTCAACACCTGGCCAAGAATTCTACGGTTTCTATCACGATTTTTTTCGATAAGGTTCCCGTTTCT
GATGCAGCAGATGTCATACTGTTTGAAAAGCCAGAAATTCGCAAAAAACTCTTAACAGCAGGTGATGACTACCAGTTACT
TTTCACTGTTCCTAAAGAACATCCCGTGACAAGAAAATATCCGGTGATTGGTTTTGTTTCAGAAGGTATGGGGGAGGTTA
GAGTGCTTGACTCGTTCGGAAGAGAGATCTATTTTGACAATTCAGGCTTTGTACATCGTTGA

Upstream 100 bases:

>100_bases
TTGTGCTTGTTGTTTTTATGTTGCATAAAGCGTACAATTGCGGTCTCACATGGCGATGTAGCTCAGCTGGTTAGAGCGGC
GGAATCATAATCCGTAGGTC

Downstream 100 bases:

>100_bases
ACTAGGCAGTATGGTTGAGGTGGAGGTAGGAAAATTCTCCACGCAGCTTTAGGCCCAGCTTCCTGTTGTAAATACGTACA
AGTATCAGTTAATTAGCAAC

Product: thiamine-monophosphate kinase

Products: NA

Alternate protein names: Thiamine-phosphate kinase [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MGSSPTFAISFFMDEHRILAEIISGIAEPLFLLDDAFVLDDFVITKDLLVEDIHFFPFAEPYKLAKKALRVNLSDLAAMG
AEPVGYFLGLVLGEKHKNSTWLNRFADGLLDDQRCYGIKLFGGDTVIHSGPLLISVTMIGKKAERVLTRSGAEIGDELCV
SGPIGDSHLGLLSYQGELQENSYFRSKYDLPEPCLDVSRDIISSVTSCIDISDGLLSEAQHLAKNSTVSITIFFDKVPVS
DAADVILFEKPEIRKKLLTAGDDYQLLFTVPKEHPVTRKYPVIGFVSEGMGEVRVLDSFGREIYFDNSGFVHR

Sequences:

>Translated_313_residues
MGSSPTFAISFFMDEHRILAEIISGIAEPLFLLDDAFVLDDFVITKDLLVEDIHFFPFAEPYKLAKKALRVNLSDLAAMG
AEPVGYFLGLVLGEKHKNSTWLNRFADGLLDDQRCYGIKLFGGDTVIHSGPLLISVTMIGKKAERVLTRSGAEIGDELCV
SGPIGDSHLGLLSYQGELQENSYFRSKYDLPEPCLDVSRDIISSVTSCIDISDGLLSEAQHLAKNSTVSITIFFDKVPVS
DAADVILFEKPEIRKKLLTAGDDYQLLFTVPKEHPVTRKYPVIGFVSEGMGEVRVLDSFGREIYFDNSGFVHR
>Mature_312_residues
GSSPTFAISFFMDEHRILAEIISGIAEPLFLLDDAFVLDDFVITKDLLVEDIHFFPFAEPYKLAKKALRVNLSDLAAMGA
EPVGYFLGLVLGEKHKNSTWLNRFADGLLDDQRCYGIKLFGGDTVIHSGPLLISVTMIGKKAERVLTRSGAEIGDELCVS
GPIGDSHLGLLSYQGELQENSYFRSKYDLPEPCLDVSRDIISSVTSCIDISDGLLSEAQHLAKNSTVSITIFFDKVPVSD
AADVILFEKPEIRKKLLTAGDDYQLLFTVPKEHPVTRKYPVIGFVSEGMGEVRVLDSFGREIYFDNSGFVHR

Specific function: Thiamine biosynthesis. [C]

COG id: COG0611

COG function: function code H; Thiamine monophosphate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine-monophosphate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1786619, Length=237, Percent_Identity=38.8185654008439, Blast_Score=133, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR016188
- InterPro:   IPR006283 [H]

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C [H]

EC number: =2.7.4.16 [H]

Molecular weight: Translated: 34651; Mature: 34520

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSSPTFAISFFMDEHRILAEIISGIAEPLFLLDDAFVLDDFVITKDLLVEDIHFFPFAE
CCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCC
PYKLAKKALRVNLSDLAAMGAEPVGYFLGLVLGEKHKNSTWLNRFADGLLDDQRCYGIKL
HHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEEE
FGGDTVIHSGPLLISVTMIGKKAERVLTRSGAEIGDELCVSGPIGDSHLGLLSYQGELQE
ECCCEEEECCCEEEEEEECCHHHHHHHHHCCHHHCCHHEECCCCCCCCEEEEEECCCCCC
NSYFRSKYDLPEPCLDVSRDIISSVTSCIDISDGLLSEAQHLAKNSTVSITIFFDKVPVS
CCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEEECCCCC
DAADVILFEKPEIRKKLLTAGDDYQLLFTVPKEHPVTRKYPVIGFVSEGMGEVRVLDSFG
CCCCEEEECCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCEEEHHHCC
REIYFDNSGFVHR
CEEEECCCCCCCC
>Mature Secondary Structure 
GSSPTFAISFFMDEHRILAEIISGIAEPLFLLDDAFVLDDFVITKDLLVEDIHFFPFAE
CCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCC
PYKLAKKALRVNLSDLAAMGAEPVGYFLGLVLGEKHKNSTWLNRFADGLLDDQRCYGIKL
HHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEEE
FGGDTVIHSGPLLISVTMIGKKAERVLTRSGAEIGDELCVSGPIGDSHLGLLSYQGELQE
ECCCEEEECCCEEEEEEECCHHHHHHHHHCCHHHCCHHEECCCCCCCCEEEEEECCCCCC
NSYFRSKYDLPEPCLDVSRDIISSVTSCIDISDGLLSEAQHLAKNSTVSITIFFDKVPVS
CCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEEECCCCC
DAADVILFEKPEIRKKLLTAGDDYQLLFTVPKEHPVTRKYPVIGFVSEGMGEVRVLDSFG
CCCCEEEECCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCEEEHHHCC
REIYFDNSGFVHR
CEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]