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The map label for this gene is ispB [H]

Identifier: 88607961

GI number: 88607961

Start: 202982

End: 203947

Strand: Direct

Name: ispB [H]

Synonym: NSE_0238

Alternate gene names: 88607961

Gene position: 202982-203947 (Clockwise)

Preceding gene: 88608551

Following gene: 88608664

Centisome position: 23.63

GC content: 40.37

Gene sequence:

>966_bases
GTGACCGCTTACGATTTTTTGAATGCATCTTTTTCCGAAAAACTGCGCTCTGTGGAAGAAGTGTTGCATGACTCTATGAC
CACTTTTACCAAGTTAAAGAGCATAGAGTATGCAAAGAAAATGCTTTCTAGTGGCAAAAGGATACGGGCTTTATTATCCC
TGCTTTCTTTTGCTCTTTGTAAAAACGCCGATACCAATGCAGAAATTCGTGCTGCAGCAACAATTGAACTAATTCACAAT
GCGACACTCATGCATGACGACGTTGTCGATGAAAACAGTATTCGCCGTGGTAAGCCTAATATAAAAAGCATTCATGGAAA
TAAAATAAGCGTTCTTACAGGTGATTTCCTTCTCAGTGTAGCGTTTGAGATTATTTTAGAGTGTAAGAATCTGAGTGTGG
TGTTGTTGCTTTCGAAAACGGCAAAGCAGCTCTCTGAGGGTGAAATGCTGCAGCTTCAGAGTATTGGTGAGATAGTTAGT
GAATCTGAGTATTTAGAGGTTATTGCAAGAAAGACAGCTGTGTTGTTTTCTGCTGCCGCAGAAATTGCAGCAATACTAAC
TGGTAAACAGGAGTATGTTGTCATCCTTAGAGAAATAGGATTTCTGATAGGCATGGCATTCCAAATTACTGATGATGTTC
TGGACTATGGGGAGCCTCAGGAAGTTGGCAAAAGTGTTGGCAACGATTTTCGAGAGTGTAAATTCACCCTACCATGTGTC
ATTGCGTACAGAGATGCAAAATCTAAGGCTGCGAAGAAATTTTGGCGCGAGGTATTTTTTACAGAGGAAAAAGATATACA
AGAAGCACGGTTTTTTATGTATGAGGTGGATGCTCTAGCAAAAGCGATGGAAGTTGCACGCAAGTACCTTAGTAAGGCAG
AAAAAAAACTCTCGATATTTGAAAACGGTGCGAGTTTACTCACAATGAAGACTTTTCTTAGGTATGCTGCTGAAAGGCCA
TACTAA

Upstream 100 bases:

>100_bases
CTACGTATGTGGTTTATTCTTTGCTTGTTGCTCAGGATCGATAGTTCCTATTTTCTTTTACTCTCTCTTTTTTGGGCAGC
GTATCATAATTTCTACGAAT

Downstream 100 bases:

>100_bases
ACTAATTAGAGTGTGTATGGCCTCTACGCTAATGATGCTAAAGTTTTTATTGTAAAGGTTCTCTTTGAGATGGTTCATAT
GTTTTCCATGTTGTAAGTGG

Product: polyprenyl synthetase family protein

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MTAYDFLNASFSEKLRSVEEVLHDSMTTFTKLKSIEYAKKMLSSGKRIRALLSLLSFALCKNADTNAEIRAAATIELIHN
ATLMHDDVVDENSIRRGKPNIKSIHGNKISVLTGDFLLSVAFEIILECKNLSVVLLLSKTAKQLSEGEMLQLQSIGEIVS
ESEYLEVIARKTAVLFSAAAEIAAILTGKQEYVVILREIGFLIGMAFQITDDVLDYGEPQEVGKSVGNDFRECKFTLPCV
IAYRDAKSKAAKKFWREVFFTEEKDIQEARFFMYEVDALAKAMEVARKYLSKAEKKLSIFENGASLLTMKTFLRYAAERP
Y

Sequences:

>Translated_321_residues
MTAYDFLNASFSEKLRSVEEVLHDSMTTFTKLKSIEYAKKMLSSGKRIRALLSLLSFALCKNADTNAEIRAAATIELIHN
ATLMHDDVVDENSIRRGKPNIKSIHGNKISVLTGDFLLSVAFEIILECKNLSVVLLLSKTAKQLSEGEMLQLQSIGEIVS
ESEYLEVIARKTAVLFSAAAEIAAILTGKQEYVVILREIGFLIGMAFQITDDVLDYGEPQEVGKSVGNDFRECKFTLPCV
IAYRDAKSKAAKKFWREVFFTEEKDIQEARFFMYEVDALAKAMEVARKYLSKAEKKLSIFENGASLLTMKTFLRYAAERP
Y
>Mature_320_residues
TAYDFLNASFSEKLRSVEEVLHDSMTTFTKLKSIEYAKKMLSSGKRIRALLSLLSFALCKNADTNAEIRAAATIELIHNA
TLMHDDVVDENSIRRGKPNIKSIHGNKISVLTGDFLLSVAFEIILECKNLSVVLLLSKTAKQLSEGEMLQLQSIGEIVSE
SEYLEVIARKTAVLFSAAAEIAAILTGKQEYVVILREIGFLIGMAFQITDDVLDYGEPQEVGKSVGNDFRECKFTLPCVI
AYRDAKSKAAKKFWREVFFTEEKDIQEARFFMYEVDALAKAMEVARKYLSKAEKKLSIFENGASLLTMKTFLRYAAERPY

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=267, Percent_Identity=32.5842696629214, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI4758430, Length=202, Percent_Identity=26.7326732673267, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI83700220, Length=202, Percent_Identity=26.7326732673267, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1789578, Length=213, Percent_Identity=40.8450704225352, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI1786623, Length=235, Percent_Identity=29.7872340425532, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17505681, Length=256, Percent_Identity=29.6875, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17508563, Length=183, Percent_Identity=30.6010928961749, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6319475, Length=275, Percent_Identity=29.0909090909091, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24651612, Length=266, Percent_Identity=28.5714285714286, Blast_Score=104, Evalue=8e-23,
Organism=Drosophila melanogaster, GI281365769, Length=224, Percent_Identity=26.3392857142857, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24660002, Length=224, Percent_Identity=26.3392857142857, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36082; Mature: 35951

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAYDFLNASFSEKLRSVEEVLHDSMTTFTKLKSIEYAKKMLSSGKRIRALLSLLSFALC
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
KNADTNAEIRAAATIELIHNATLMHDDVVDENSIRRGKPNIKSIHGNKISVLTGDFLLSV
CCCCCCCEEHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCEECCCEEEEEHHHHHHHH
AFEIILECKNLSVVLLLSKTAKQLSEGEMLQLQSIGEIVSESEYLEVIARKTAVLFSAAA
HHHHHHHHCCCEEEEEECHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIAAILTGKQEYVVILREIGFLIGMAFQITDDVLDYGEPQEVGKSVGNDFRECKFTLPCV
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHEECCEE
IAYRDAKSKAAKKFWREVFFTEEKDIQEARFFMYEVDALAKAMEVARKYLSKAEKKLSIF
EEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENGASLLTMKTFLRYAAERPY
HCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TAYDFLNASFSEKLRSVEEVLHDSMTTFTKLKSIEYAKKMLSSGKRIRALLSLLSFALC
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
KNADTNAEIRAAATIELIHNATLMHDDVVDENSIRRGKPNIKSIHGNKISVLTGDFLLSV
CCCCCCCEEHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCEECCCEEEEEHHHHHHHH
AFEIILECKNLSVVLLLSKTAKQLSEGEMLQLQSIGEIVSESEYLEVIARKTAVLFSAAA
HHHHHHHHCCCEEEEEECHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIAAILTGKQEYVVILREIGFLIGMAFQITDDVLDYGEPQEVGKSVGNDFRECKFTLPCV
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHEECCEE
IAYRDAKSKAAKKFWREVFFTEEKDIQEARFFMYEVDALAKAMEVARKYLSKAEKKLSIF
EEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENGASLLTMKTFLRYAAERPY
HCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]