Click here to switch to the map view.

The map label for this gene is rho [H]

Identifier: 88607945

GI number: 88607945

Start: 1135797

End: 1137128

Strand: Reverse

Name: rho [H]

Synonym: APH_1071

Alternate gene names: 88607945

Gene position: 1137128-1135797 (Counterclockwise)

Preceding gene: 88607228

Following gene: 88607498

Centisome position: 77.29

GC content: 44.14

Gene sequence:

>1332_bases
ATGGAAAGAGAAGAGTCTGTAGTAGCAGAAGAGGCGACAGGGAGTGGCAGCGAAGAAGGAAGAATCCTGAACCTATGTGA
GCTGAAGAGAAAAAGCACAGGGGAGCTACTAGCAATAGCGGAGGAGCTAGGTGTAGTAAGCAACGGCAGGATGCTGAAGC
AAGAGATAATATTCCAGCTGATGAAGAGAGTAATCAGCGAAGGAGGAGTGGCAATAGGTGGTGGTGTAGTAGAAACATTG
CCGGATGGATTTGGATTTTTGAGATCAGCAGAAGCAAATTACGCGGCAAGTAGCGATGACATCTACATATCGGCTGGTCA
GATAAAGAAATTTAACTTGAGAACGGGTGACATAGTAAGTGGTGAGATACGTGCACCTGGAGATAAGGAAAGGTATTTTA
CCTTAGTCAAGGCGTACAGCATAAACTACACGGAGATAGGTAAGCTACAGAGGTATGTACACTTTGACGATCTGATTCCA
GTATACCCAGAAGATAGAATATTGCTCGAGTGCAATAAGGGTGCTGGGAACGAGAAGAAAGATATAAGCATGCGTGCTAT
AGACATAATAGCGCCACTAGGAAAGGGGCAACGTGCGCTAATAGTAGCACCTCCAAGGGTTGGTAAGACTGTAATCTTGC
AGCAAATCGCGCACTCTATAGCTGTAAACCACCCCAACATGGAACTGATAGTGCTTTTGATAGGTGAAAGGCCAGAAGAA
GTCACGGATATGTTGAGGTCAGTAAAGGGAGAAGTTGTAAGTTCTACATTTGATGAGCCTGCGTACCGGCACGTACAGTT
GGCAGAGATAGTGATAGAAAGAGCTAAGAGGATGGTAGAGCATAAGAAGGAGGTGGTAATCCTTTTAGACTCTATAACAA
GATTAGCTAGGGCATATAATGAGGTTATGCCATCATCAGGAAAGGTGCTTACAGGTGGGGTAGATTCTAATGCGCTACAG
AGGCCTAAGAGGTTTTTTGGAGCAGCTAGGAATATAGAGAATGGGGGATCGCTCACGATAATAGCGACTGCTCTAATAGA
GACGGGATCGAAAATGGATGAAGTGATTTTCGAGGAGTTCAAGGGTACAGGAAACTGCGAGATAATACTTGATCGGAAGA
TAGCAGATAAGAGAATCTACCCAGCGATAGATATATCTAAGTCTGGAACAAGAAAGGAAGACATGCTGATAGAATCAGCG
CTTCTGAAGAAGGTATGGTTACTGAGAAGGTTGTTATCAGCCATGGGTCCAGTTGAGGCTATGGAGTTTCTGAGAGACAA
GCTTAGCATGGCTAAGGATAACAACGACTTTTTTGAAATGATGAATAGCTAA

Upstream 100 bases:

>100_bases
CCTTTGTTATGAGCAGGGTTAGTGATGTCACTGCTATGGGGGATTTAGGGGGTTATACTTTTTAGGTTTAGTGAAGAGAG
TTGGTATGTCAGAAGGTGTC

Downstream 100 bases:

>100_bases
CGGTTATCTGTACGGGTACGATTAGGCGTAACGATGGCTGCAGCATCGTTTGGTGTCTACACCTAGGAAAGACTGGGTAT
GGTTAACTATCCAGAGGTTC

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho 1 [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MEREESVVAEEATGSGSEEGRILNLCELKRKSTGELLAIAEELGVVSNGRMLKQEIIFQLMKRVISEGGVAIGGGVVETL
PDGFGFLRSAEANYAASSDDIYISAGQIKKFNLRTGDIVSGEIRAPGDKERYFTLVKAYSINYTEIGKLQRYVHFDDLIP
VYPEDRILLECNKGAGNEKKDISMRAIDIIAPLGKGQRALIVAPPRVGKTVILQQIAHSIAVNHPNMELIVLLIGERPEE
VTDMLRSVKGEVVSSTFDEPAYRHVQLAEIVIERAKRMVEHKKEVVILLDSITRLARAYNEVMPSSGKVLTGGVDSNALQ
RPKRFFGAARNIENGGSLTIIATALIETGSKMDEVIFEEFKGTGNCEIILDRKIADKRIYPAIDISKSGTRKEDMLIESA
LLKKVWLLRRLLSAMGPVEAMEFLRDKLSMAKDNNDFFEMMNS

Sequences:

>Translated_443_residues
MEREESVVAEEATGSGSEEGRILNLCELKRKSTGELLAIAEELGVVSNGRMLKQEIIFQLMKRVISEGGVAIGGGVVETL
PDGFGFLRSAEANYAASSDDIYISAGQIKKFNLRTGDIVSGEIRAPGDKERYFTLVKAYSINYTEIGKLQRYVHFDDLIP
VYPEDRILLECNKGAGNEKKDISMRAIDIIAPLGKGQRALIVAPPRVGKTVILQQIAHSIAVNHPNMELIVLLIGERPEE
VTDMLRSVKGEVVSSTFDEPAYRHVQLAEIVIERAKRMVEHKKEVVILLDSITRLARAYNEVMPSSGKVLTGGVDSNALQ
RPKRFFGAARNIENGGSLTIIATALIETGSKMDEVIFEEFKGTGNCEIILDRKIADKRIYPAIDISKSGTRKEDMLIESA
LLKKVWLLRRLLSAMGPVEAMEFLRDKLSMAKDNNDFFEMMNS
>Mature_443_residues
MEREESVVAEEATGSGSEEGRILNLCELKRKSTGELLAIAEELGVVSNGRMLKQEIIFQLMKRVISEGGVAIGGGVVETL
PDGFGFLRSAEANYAASSDDIYISAGQIKKFNLRTGDIVSGEIRAPGDKERYFTLVKAYSINYTEIGKLQRYVHFDDLIP
VYPEDRILLECNKGAGNEKKDISMRAIDIIAPLGKGQRALIVAPPRVGKTVILQQIAHSIAVNHPNMELIVLLIGERPEE
VTDMLRSVKGEVVSSTFDEPAYRHVQLAEIVIERAKRMVEHKKEVVILLDSITRLARAYNEVMPSSGKVLTGGVDSNALQ
RPKRFFGAARNIENGGSLTIIATALIETGSKMDEVIFEEFKGTGNCEIILDRKIADKRIYPAIDISKSGTRKEDMLIESA
LLKKVWLLRRLLSAMGPVEAMEFLRDKLSMAKDNNDFFEMMNS

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=419, Percent_Identity=59.6658711217184, Blast_Score=524, Evalue=1e-150,
Organism=Escherichia coli, GI1788251, Length=244, Percent_Identity=28.6885245901639, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 49063; Mature: 49063

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEREESVVAEEATGSGSEEGRILNLCELKRKSTGELLAIAEELGVVSNGRMLKQEIIFQL
CCCCHHHHHHHCCCCCCCCCCEEHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
MKRVISEGGVAIGGGVVETLPDGFGFLRSAEANYAASSDDIYISAGQIKKFNLRTGDIVS
HHHHHHCCCEEECCCHHHHCCCCHHHHHCCCCCCCCCCCCEEEECCCEEEEECCCCCEEE
GEIRAPGDKERYFTLVKAYSINYTEIGKLQRYVHFDDLIPVYPEDRILLECNKGAGNEKK
CCCCCCCCHHHHEEEEHEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCC
DISMRAIDIIAPLGKGQRALIVAPPRVGKTVILQQIAHSIAVNHPNMELIVLLIGERPEE
CCHHHHEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHH
VTDMLRSVKGEVVSSTFDEPAYRHVQLAEIVIERAKRMVEHKKEVVILLDSITRLARAYN
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHEEEHHHHHHHHHHHHH
EVMPSSGKVLTGGVDSNALQRPKRFFGAARNIENGGSLTIIATALIETGSKMDEVIFEEF
HHCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHCCCHHHHHHHHHH
KGTGNCEIILDRKIADKRIYPAIDISKSGTRKEDMLIESALLKKVWLLRRLLSAMGPVEA
CCCCCEEEEEECCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
MEFLRDKLSMAKDNNDFFEMMNS
HHHHHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure
MEREESVVAEEATGSGSEEGRILNLCELKRKSTGELLAIAEELGVVSNGRMLKQEIIFQL
CCCCHHHHHHHCCCCCCCCCCEEHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
MKRVISEGGVAIGGGVVETLPDGFGFLRSAEANYAASSDDIYISAGQIKKFNLRTGDIVS
HHHHHHCCCEEECCCHHHHCCCCHHHHHCCCCCCCCCCCCEEEECCCEEEEECCCCCEEE
GEIRAPGDKERYFTLVKAYSINYTEIGKLQRYVHFDDLIPVYPEDRILLECNKGAGNEKK
CCCCCCCCHHHHEEEEHEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCC
DISMRAIDIIAPLGKGQRALIVAPPRVGKTVILQQIAHSIAVNHPNMELIVLLIGERPEE
CCHHHHEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHH
VTDMLRSVKGEVVSSTFDEPAYRHVQLAEIVIERAKRMVEHKKEVVILLDSITRLARAYN
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHEEEHHHHHHHHHHHHH
EVMPSSGKVLTGGVDSNALQRPKRFFGAARNIENGGSLTIIATALIETGSKMDEVIFEEF
HHCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHCCCHHHHHHHHHH
KGTGNCEIILDRKIADKRIYPAIDISKSGTRKEDMLIESALLKKVWLLRRLLSAMGPVEA
CCCCCEEEEEECCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
MEFLRDKLSMAKDNNDFFEMMNS
HHHHHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA