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The map label for this gene is clpB [H]

Identifier: 88607892

GI number: 88607892

Start: 233666

End: 236245

Strand: Reverse

Name: clpB [H]

Synonym: APH_0235

Alternate gene names: 88607892

Gene position: 236245-233666 (Counterclockwise)

Preceding gene: 88607762

Following gene: 88607014

Centisome position: 16.06

GC content: 42.33

Gene sequence:

>2580_bases
ATGGACTTGAATAAGTTTACTGAAAAGGCCAGAAGCTTCGTGATGCAGGCACAGGTGTTTGCCATATCTTCAGGACATCA
GTTTCTTTTGCCTGAACACTTACTGAAGGTAATGTTAGATGAAAAAGGCAGTTTGACTGAAAACCTTATATCAATGTCAT
CCTGCAATGCAGGCATAAACGAGATTCGCGACGCACTCGTACAACATCTGAGCAAATTGCCAGTAGTTAGCGGATCTAGC
AGCGGACAACTAAATCTCTCAAGAGAATTGGCTCAGGTTCTGGAAGAAGCAAGCAACATTGCTAAAAGAAACGGCGATGC
GTACATCTCTGCTGAAAGGTTGTTACAAGCACTCGTAGTGGTTAACAGCAACGTTTCAAGAGTGCTAATAAATGCTGGAG
TTACTGCTACAAAACTCAATACTCTAATAGAAAAAATGCGCGAAGGAGACAAAGCGGATAGCGAAACCGCGGAACAAAAA
TTCGATGCTCTGAGAAAGTTTACGCAAGACCTCACAGAACTCGCTACTGAAGGTAAAATGGACCCTGTAATCGGTAGAAG
TGATGAAACTAGACGCCTCATACAAGTGCTCTCACGCAGAACAAAAAACAATCCTGCCCTAATTGGTGAACCTGGCGTAG
GTAAGAGTGCTATCGTAGAAGGGTTAGTGCAATCAATAGTTTCCGGAAATGTCCCCATAGGGCTGCAAGGCGCCAAGGTA
TTATCGCTAGATTTAGCTGCATTGGTTGCGGGCACTAAGTATCGTGGTGAATTTGAAGAGCGTCTTAAAGCCGTACTATC
AAAAATTATCCTATCTAGCGGTAAAATCATACTGTTTATAGATGAGTTACACATGCTGGTAGGCGCTGGATCTACTGGTG
ATTCCATGGATGCCTCTAACATATTGAAACCAGTTCTTGCGCGGGGTGAACTACGCTGTATTGGTGCAACAACTCTAGAT
GAGTATAGAGAACATATAGAAAAAGATCCTGCTCTCGCTAGGAGATTTCAACCAGTATTTGTGGCAGAACCCAGCATTAA
CGATACTATCTCAATACTAAGAGGGATTAAGGAAAAATATGAGCTGCACCACGGTATTAGGATCACTGACAGCGCTATAG
TAGCAGCAGCAAACTTATCTAGCAGATACATACCTGACAGATTTTTACCTGACAAGGCAATTGACTTAATAGATGAAGCT
GCAAGTAGAGCAAGGATCGAAATTGATAGCAAGCCAGAAATAATAGACAGTATTGATCGTCAGGTAATGCAGCTAAAAAT
AGAGTCTGAAGCACTGAAAAACGAAAATACTGAGGCATCAAAGCAGCGCTTAGAGGAGATTAGCCGGGAATTGCAAAGCC
TAAGTTCCGAAGCTGCTGATTTAAACAGCCAATGGCACGCTGAAAAAGCAAAGATCTCAAAAATGCACGAGCTAACAGAA
TCCCTCGATAGTGCCCGTATAGAATTAGAGCAGTCACAAAGACTGGGCAATCTTTCCAGGGCAGGAGAATTAATGTACGG
AATCATTCCATCCTTAGAAGCAGAACTGAAGAAACATGAAGAAATCGCAGGGACGCTCCTCAGGAAGGAAATAAAAGCAA
ATGATATAGCGGCAATCGTAGAAAGGTGGACTGGTATTCCAGTGGATAGCGTTATGAATAGTGAAAAGGAAAAGCTATTA
CACATGGAAGAAGAGCTCAAGAAGACAGTAATAGGACAGGATAGTGCTGTAGCAGCTGTGAGTAATGCAGTTCGGAGATC
ACGTGCTGGAGTACAAGATGCTCAAAGACCTATGGGTTCCTTCTTATTTCTAGGTCCTACTGGCGTGGGCAAAACAGAAC
TCACGAAAGCTCTAAGCAAGTTCCTATTCGACTCAAGTTCTGCTCTACTGCGCTTCGATATGTCGGAGTTTATGGAAAAA
CATTCTGTGGCAAAACTGATAGGAGCGCCTCCTGGATACGTGGGATATGAACAAGGTGGACTACTGACTGAAGCCGTTCG
CAGGAGACCGTATCAGGTGATATTGTTCGATGAAATAGAAAAAGCCCACGCAGATATCTTCAATTTGCTCTTACAAGTCC
TAGACGAAGGAAGATTGACAGACAGCCGTGGGAATTTAGTAAACTTCAAAAATACGATACTAGTGCTAACGTCAAACATA
GGTCAGGACATACTAATAAATAGCACGGAGGACAGTAATGATCCTGTAGTACGGAAAACCGTGTTGGAAATGCTGCGCCT
ATCCTTTCGTCCAGAATTTCTGAACCGGCTTGATGAGATCATGATATTCAATAGACTGACTCAGGAACATATAGAGCATA
TTGTGGATGTACAGATATCGAATCTACAAAAAATTATCAGCGACAAAGGCATTACAATTTCTCTGCATCAAGGAGCAAAA
TCGTGGCTCGTTAAGCATGGGTATGATGTAGCATGTGGAGCACGCCTATTAAAACGTCTAATACAGCAACACATTCAAAA
TCAACTTGCATGCTTATTGCTAGGAGACAAAATAACAGAAGGTAGCAAGCTTGTGGTATTTGAAGAAAATAATTCTCTAG
TTATCAAAGAGGCTACATAA

Upstream 100 bases:

>100_bases
GTGGAAGGTACCAATTTTTAATTTGATTTACTATTTATTTTTTGCCAATGAATACATACCTACTATGTATGAGATTCTTT
AGAGCAGGAGATTCACCTAT

Downstream 100 bases:

>100_bases
CAAAATGTAGTAACATTGCTTGAAAGCGAGAAATATGCGTCAAGGGAGGATTACAATACTACAAGACGGTTGCGTTACGC
CGCAGCCTTTGTCACTGTAT

Product: ATP-dependent chaperone protein ClpB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 859; Mature: 859

Protein sequence:

>859_residues
MDLNKFTEKARSFVMQAQVFAISSGHQFLLPEHLLKVMLDEKGSLTENLISMSSCNAGINEIRDALVQHLSKLPVVSGSS
SGQLNLSRELAQVLEEASNIAKRNGDAYISAERLLQALVVVNSNVSRVLINAGVTATKLNTLIEKMREGDKADSETAEQK
FDALRKFTQDLTELATEGKMDPVIGRSDETRRLIQVLSRRTKNNPALIGEPGVGKSAIVEGLVQSIVSGNVPIGLQGAKV
LSLDLAALVAGTKYRGEFEERLKAVLSKIILSSGKIILFIDELHMLVGAGSTGDSMDASNILKPVLARGELRCIGATTLD
EYREHIEKDPALARRFQPVFVAEPSINDTISILRGIKEKYELHHGIRITDSAIVAAANLSSRYIPDRFLPDKAIDLIDEA
ASRARIEIDSKPEIIDSIDRQVMQLKIESEALKNENTEASKQRLEEISRELQSLSSEAADLNSQWHAEKAKISKMHELTE
SLDSARIELEQSQRLGNLSRAGELMYGIIPSLEAELKKHEEIAGTLLRKEIKANDIAAIVERWTGIPVDSVMNSEKEKLL
HMEEELKKTVIGQDSAVAAVSNAVRRSRAGVQDAQRPMGSFLFLGPTGVGKTELTKALSKFLFDSSSALLRFDMSEFMEK
HSVAKLIGAPPGYVGYEQGGLLTEAVRRRPYQVILFDEIEKAHADIFNLLLQVLDEGRLTDSRGNLVNFKNTILVLTSNI
GQDILINSTEDSNDPVVRKTVLEMLRLSFRPEFLNRLDEIMIFNRLTQEHIEHIVDVQISNLQKIISDKGITISLHQGAK
SWLVKHGYDVACGARLLKRLIQQHIQNQLACLLLGDKITEGSKLVVFEENNSLVIKEAT

Sequences:

>Translated_859_residues
MDLNKFTEKARSFVMQAQVFAISSGHQFLLPEHLLKVMLDEKGSLTENLISMSSCNAGINEIRDALVQHLSKLPVVSGSS
SGQLNLSRELAQVLEEASNIAKRNGDAYISAERLLQALVVVNSNVSRVLINAGVTATKLNTLIEKMREGDKADSETAEQK
FDALRKFTQDLTELATEGKMDPVIGRSDETRRLIQVLSRRTKNNPALIGEPGVGKSAIVEGLVQSIVSGNVPIGLQGAKV
LSLDLAALVAGTKYRGEFEERLKAVLSKIILSSGKIILFIDELHMLVGAGSTGDSMDASNILKPVLARGELRCIGATTLD
EYREHIEKDPALARRFQPVFVAEPSINDTISILRGIKEKYELHHGIRITDSAIVAAANLSSRYIPDRFLPDKAIDLIDEA
ASRARIEIDSKPEIIDSIDRQVMQLKIESEALKNENTEASKQRLEEISRELQSLSSEAADLNSQWHAEKAKISKMHELTE
SLDSARIELEQSQRLGNLSRAGELMYGIIPSLEAELKKHEEIAGTLLRKEIKANDIAAIVERWTGIPVDSVMNSEKEKLL
HMEEELKKTVIGQDSAVAAVSNAVRRSRAGVQDAQRPMGSFLFLGPTGVGKTELTKALSKFLFDSSSALLRFDMSEFMEK
HSVAKLIGAPPGYVGYEQGGLLTEAVRRRPYQVILFDEIEKAHADIFNLLLQVLDEGRLTDSRGNLVNFKNTILVLTSNI
GQDILINSTEDSNDPVVRKTVLEMLRLSFRPEFLNRLDEIMIFNRLTQEHIEHIVDVQISNLQKIISDKGITISLHQGAK
SWLVKHGYDVACGARLLKRLIQQHIQNQLACLLLGDKITEGSKLVVFEENNSLVIKEAT
>Mature_859_residues
MDLNKFTEKARSFVMQAQVFAISSGHQFLLPEHLLKVMLDEKGSLTENLISMSSCNAGINEIRDALVQHLSKLPVVSGSS
SGQLNLSRELAQVLEEASNIAKRNGDAYISAERLLQALVVVNSNVSRVLINAGVTATKLNTLIEKMREGDKADSETAEQK
FDALRKFTQDLTELATEGKMDPVIGRSDETRRLIQVLSRRTKNNPALIGEPGVGKSAIVEGLVQSIVSGNVPIGLQGAKV
LSLDLAALVAGTKYRGEFEERLKAVLSKIILSSGKIILFIDELHMLVGAGSTGDSMDASNILKPVLARGELRCIGATTLD
EYREHIEKDPALARRFQPVFVAEPSINDTISILRGIKEKYELHHGIRITDSAIVAAANLSSRYIPDRFLPDKAIDLIDEA
ASRARIEIDSKPEIIDSIDRQVMQLKIESEALKNENTEASKQRLEEISRELQSLSSEAADLNSQWHAEKAKISKMHELTE
SLDSARIELEQSQRLGNLSRAGELMYGIIPSLEAELKKHEEIAGTLLRKEIKANDIAAIVERWTGIPVDSVMNSEKEKLL
HMEEELKKTVIGQDSAVAAVSNAVRRSRAGVQDAQRPMGSFLFLGPTGVGKTELTKALSKFLFDSSSALLRFDMSEFMEK
HSVAKLIGAPPGYVGYEQGGLLTEAVRRRPYQVILFDEIEKAHADIFNLLLQVLDEGRLTDSRGNLVNFKNTILVLTSNI
GQDILINSTEDSNDPVVRKTVLEMLRLSFRPEFLNRLDEIMIFNRLTQEHIEHIVDVQISNLQKIISDKGITISLHQGAK
SWLVKHGYDVACGARLLKRLIQQHIQNQLACLLLGDKITEGSKLVVFEENNSLVIKEAT

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=326, Percent_Identity=35.2760736196319, Blast_Score=199, Evalue=7e-51,
Organism=Escherichia coli, GI1788943, Length=858, Percent_Identity=52.5641025641026, Blast_Score=908, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=323, Percent_Identity=44.8916408668731, Blast_Score=286, Evalue=3e-78,
Organism=Saccharomyces cerevisiae, GI6320464, Length=701, Percent_Identity=54.0656205420827, Blast_Score=769, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=859, Percent_Identity=40.9778812572759, Blast_Score=633, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: 3.4.21.92

Molecular weight: Translated: 94998; Mature: 94998

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLNKFTEKARSFVMQAQVFAISSGHQFLLPEHLLKVMLDEKGSLTENLISMSSCNAGIN
CCHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCHH
EIRDALVQHLSKLPVVSGSSSGQLNLSRELAQVLEEASNIAKRNGDAYISAERLLQALVV
HHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHH
VNSNVSRVLINAGVTATKLNTLIEKMREGDKADSETAEQKFDALRKFTQDLTELATEGKM
HCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
DPVIGRSDETRRLIQVLSRRTKNNPALIGEPGVGKSAIVEGLVQSIVSGNVPIGLQGAKV
CCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHH
LSLDLAALVAGTKYRGEFEERLKAVLSKIILSSGKIILFIDELHMLVGAGSTGDSMDASN
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCCCCCHHH
ILKPVLARGELRCIGATTLDEYREHIEKDPALARRFQPVFVAEPSINDTISILRGIKEKY
HHHHHHCCCCEEEECCHHHHHHHHHHHCCHHHHHHCCCEEEECCCCCHHHHHHHHHHHHH
ELHHGIRITDSAIVAAANLSSRYIPDRFLPDKAIDLIDEAASRARIEIDSKPEIIDSIDR
HHHCCCEEECCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCEEEECCCCHHHHHHHH
QVMQLKIESEALKNENTEASKQRLEEISRELQSLSSEAADLNSQWHAEKAKISKMHELTE
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
SLDSARIELEQSQRLGNLSRAGELMYGIIPSLEAELKKHEEIAGTLLRKEIKANDIAAIV
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERWTGIPVDSVMNSEKEKLLHMEEELKKTVIGQDSAVAAVSNAVRRSRAGVQDAQRPMGS
HHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHCCCCC
FLFLGPTGVGKTELTKALSKFLFDSSSALLRFDMSEFMEKHSVAKLIGAPPGYVGYEQGG
EEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
LLTEAVRRRPYQVILFDEIEKAHADIFNLLLQVLDEGRLTDSRGNLVNFKNTILVLTSNI
HHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCEEEEEECCC
GQDILINSTEDSNDPVVRKTVLEMLRLSFRPEFLNRLDEIMIFNRLTQEHIEHIVDVQIS
CCCEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLQKIISDKGITISLHQGAKSWLVKHGYDVACGARLLKRLIQQHIQNQLACLLLGDKITE
HHHHHHCCCCCEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEHHCCCCCCC
GSKLVVFEENNSLVIKEAT
CCEEEEEECCCCEEEEECC
>Mature Secondary Structure
MDLNKFTEKARSFVMQAQVFAISSGHQFLLPEHLLKVMLDEKGSLTENLISMSSCNAGIN
CCHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCHH
EIRDALVQHLSKLPVVSGSSSGQLNLSRELAQVLEEASNIAKRNGDAYISAERLLQALVV
HHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHH
VNSNVSRVLINAGVTATKLNTLIEKMREGDKADSETAEQKFDALRKFTQDLTELATEGKM
HCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
DPVIGRSDETRRLIQVLSRRTKNNPALIGEPGVGKSAIVEGLVQSIVSGNVPIGLQGAKV
CCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHH
LSLDLAALVAGTKYRGEFEERLKAVLSKIILSSGKIILFIDELHMLVGAGSTGDSMDASN
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCCCCCHHH
ILKPVLARGELRCIGATTLDEYREHIEKDPALARRFQPVFVAEPSINDTISILRGIKEKY
HHHHHHCCCCEEEECCHHHHHHHHHHHCCHHHHHHCCCEEEECCCCCHHHHHHHHHHHHH
ELHHGIRITDSAIVAAANLSSRYIPDRFLPDKAIDLIDEAASRARIEIDSKPEIIDSIDR
HHHCCCEEECCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCEEEECCCCHHHHHHHH
QVMQLKIESEALKNENTEASKQRLEEISRELQSLSSEAADLNSQWHAEKAKISKMHELTE
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
SLDSARIELEQSQRLGNLSRAGELMYGIIPSLEAELKKHEEIAGTLLRKEIKANDIAAIV
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERWTGIPVDSVMNSEKEKLLHMEEELKKTVIGQDSAVAAVSNAVRRSRAGVQDAQRPMGS
HHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHCCCCC
FLFLGPTGVGKTELTKALSKFLFDSSSALLRFDMSEFMEKHSVAKLIGAPPGYVGYEQGG
EEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
LLTEAVRRRPYQVILFDEIEKAHADIFNLLLQVLDEGRLTDSRGNLVNFKNTILVLTSNI
HHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCEEEEEECCC
GQDILINSTEDSNDPVVRKTVLEMLRLSFRPEFLNRLDEIMIFNRLTQEHIEHIVDVQIS
CCCEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLQKIISDKGITISLHQGAKSWLVKHGYDVACGARLLKRLIQQHIQNQLACLLLGDKITE
HHHHHHCCCCCEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEHHCCCCCCC
GSKLVVFEENNSLVIKEAT
CCEEEEEECCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA