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The map label for this gene is pmbA [H]

Identifier: 88607884

GI number: 88607884

Start: 725959

End: 727290

Strand: Reverse

Name: pmbA [H]

Synonym: APH_0705

Alternate gene names: 88607884

Gene position: 727290-725959 (Counterclockwise)

Preceding gene: 88606705

Following gene: 88607167

Centisome position: 49.43

GC content: 40.24

Gene sequence:

>1332_bases
TTGAGTGCCCTGAATATAGCAGAAGATATAATTAAACTTATAAAGTCTCGGGGAGTTGAGGGAGAAGTCGTATTAACTCA
GCACGAGACTACCTCTGTTACCCAGCGTATGATGAGGCTCGAGGAAATGATTGCGAGCAGCGGATGTAATGTTGGGATAC
GCGTGATTGTGGGTAAGAAGTATTCATGTATTTCATCGAATGATTTAGAGAGGTCTGAAGAGCTAGTAGATGCTGCTGTA
AAGATGGCAGACTTATCTCCTGAGGATCCATTTATCTCTGTAAGTGATCAACAAGGAACGTATACCAATACTGCTTCTGA
TCTTATGATCTTCGATGATTCTATTGTTGAGATGGATTCTATACGTGACATTCTTCAGGAAATGGAGGGTGAAGCCCTGT
CGGTGGATTCTAGAATAGTAAATTCTGAAGGAGCAAGTTTCTCGAAGTCGTTGACGGAGACTGCCCTTATGACAACAAGA
GGGTTTTGTGGTTCATATAAGAAAACATATTTCTCTTCATCTATATCTGTAGTAGCGGCATCTGATGATGAGAAGATGGA
GGTAGACTATAGCTTTGCGGTGAAGAGTAAATTCAGTGATTTAGAGAAGTCGAGGGTTTTAGGGAGAGAAGCTGCTAATC
GGGCTCTGCGGAAGCTGAATGCTCGCGAGCTTCAAACATGTAAAATGCCAGTTTTATTTGAAAATAGGGTGGCATCTTCA
TTGTTGTCCAATTTTGCTTCAGCTATTAATGGAGCTTCTATCGCTGATAAGACGTCTTTTCTTCTAAATGATTTAGGAAC
TAATGTTTTTGGCAGTGATATTTATATAGTGGATGATCCTCTCATGGTATCTGGTATATCATCGCGTCCCTTTGATGGTG
AGGGTATACTGTCTACAAAGCGGAGCATAGTAGAAAGAGGTGTTCTCAAGAGTTGGATTTTAGATATGAGAACGGCAAAA
CAACTGGCATTGTCCACGACAGGTCATGCGGTAAGGAAAGATAATGCGTCTGTTTCTCCCGGTGTAAGTAATTTTTACAT
ACAGAGATCGGATATTACAGTACAGGAATTAATGTCAGATATTAAGAGAGGGTTGTACATCACTGATCTTTTCGGATTTG
GAATAAATTTTGCTACAGGAGATTATAGTCAAGGAGCTTTTGGTTATATGATCGAAAATGGAGATATAACCTATCCAGTC
CATGGAATTACTATAGCAGGGAATCTACGAGATATGTTTTCAAGCGCACAAGTTGCGAACGATTTAATTTTTCTCCGATC
AGTAAACTCTCCGACATTAAGGTTCGGTGATATGGTTGTTTCTGGTGCTTAG

Upstream 100 bases:

>100_bases
GAATGTACAAACTAGTCAAATGTTAGGGGTTTTTGAAGAGCGTTGTGATTTGCGCAATGAATTCTTCTTAAGAATTAAAA
TGTATAAGTAGAGGTGTGTC

Downstream 100 bases:

>100_bases
TAAACCATCAATATCAGTAGCGTTTTACCTTGTTAAAGCAAGTGGTAGTTATTCATGTAAATCCTACATCAAACAGTCAT
ATGGGAATAAGATGAAATTG

Product: pmbA protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MSALNIAEDIIKLIKSRGVEGEVVLTQHETTSVTQRMMRLEEMIASSGCNVGIRVIVGKKYSCISSNDLERSEELVDAAV
KMADLSPEDPFISVSDQQGTYTNTASDLMIFDDSIVEMDSIRDILQEMEGEALSVDSRIVNSEGASFSKSLTETALMTTR
GFCGSYKKTYFSSSISVVAASDDEKMEVDYSFAVKSKFSDLEKSRVLGREAANRALRKLNARELQTCKMPVLFENRVASS
LLSNFASAINGASIADKTSFLLNDLGTNVFGSDIYIVDDPLMVSGISSRPFDGEGILSTKRSIVERGVLKSWILDMRTAK
QLALSTTGHAVRKDNASVSPGVSNFYIQRSDITVQELMSDIKRGLYITDLFGFGINFATGDYSQGAFGYMIENGDITYPV
HGITIAGNLRDMFSSAQVANDLIFLRSVNSPTLRFGDMVVSGA

Sequences:

>Translated_443_residues
MSALNIAEDIIKLIKSRGVEGEVVLTQHETTSVTQRMMRLEEMIASSGCNVGIRVIVGKKYSCISSNDLERSEELVDAAV
KMADLSPEDPFISVSDQQGTYTNTASDLMIFDDSIVEMDSIRDILQEMEGEALSVDSRIVNSEGASFSKSLTETALMTTR
GFCGSYKKTYFSSSISVVAASDDEKMEVDYSFAVKSKFSDLEKSRVLGREAANRALRKLNARELQTCKMPVLFENRVASS
LLSNFASAINGASIADKTSFLLNDLGTNVFGSDIYIVDDPLMVSGISSRPFDGEGILSTKRSIVERGVLKSWILDMRTAK
QLALSTTGHAVRKDNASVSPGVSNFYIQRSDITVQELMSDIKRGLYITDLFGFGINFATGDYSQGAFGYMIENGDITYPV
HGITIAGNLRDMFSSAQVANDLIFLRSVNSPTLRFGDMVVSGA
>Mature_442_residues
SALNIAEDIIKLIKSRGVEGEVVLTQHETTSVTQRMMRLEEMIASSGCNVGIRVIVGKKYSCISSNDLERSEELVDAAVK
MADLSPEDPFISVSDQQGTYTNTASDLMIFDDSIVEMDSIRDILQEMEGEALSVDSRIVNSEGASFSKSLTETALMTTRG
FCGSYKKTYFSSSISVVAASDDEKMEVDYSFAVKSKFSDLEKSRVLGREAANRALRKLNARELQTCKMPVLFENRVASSL
LSNFASAINGASIADKTSFLLNDLGTNVFGSDIYIVDDPLMVSGISSRPFDGEGILSTKRSIVERGVLKSWILDMRTAKQ
LALSTTGHAVRKDNASVSPGVSNFYIQRSDITVQELMSDIKRGLYITDLFGFGINFATGDYSQGAFGYMIENGDITYPVH
GITIAGNLRDMFSSAQVANDLIFLRSVNSPTLRFGDMVVSGA

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=424, Percent_Identity=33.0188679245283, Blast_Score=238, Evalue=6e-64,
Organism=Escherichia coli, GI1789640, Length=345, Percent_Identity=24.6376811594203, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48329; Mature: 48198

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALNIAEDIIKLIKSRGVEGEVVLTQHETTSVTQRMMRLEEMIASSGCNVGIRVIVGKK
CCCHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
YSCISSNDLERSEELVDAAVKMADLSPEDPFISVSDQQGTYTNTASDLMIFDDSIVEMDS
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCEEEECCCHHHHHH
IRDILQEMEGEALSVDSRIVNSEGASFSKSLTETALMTTRGFCGSYKKTYFSSSISVVAA
HHHHHHHHCCCEEECCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEEEE
SDDEKMEVDYSFAVKSKFSDLEKSRVLGREAANRALRKLNARELQTCKMPVLFENRVASS
CCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHH
LLSNFASAINGASIADKTSFLLNDLGTNVFGSDIYIVDDPLMVSGISSRPFDGEGILSTK
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCEEECCCCCCCCCCCHHHHH
RSIVERGVLKSWILDMRTAKQLALSTTGHAVRKDNASVSPGVSNFYIQRSDITVQELMSD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCEEEEECCCCHHHHHHH
IKRGLYITDLFGFGINFATGDYSQGAFGYMIENGDITYPVHGITIAGNLRDMFSSAQVAN
HHCCEEEEEHHHCCCEECCCCCCCCCEEEEEECCCEEEEEEEEEEECCHHHHHHHHHHHH
DLIFLRSVNSPTLRFGDMVVSGA
HEEEEEECCCCCEEECCEEECCC
>Mature Secondary Structure 
SALNIAEDIIKLIKSRGVEGEVVLTQHETTSVTQRMMRLEEMIASSGCNVGIRVIVGKK
CCHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
YSCISSNDLERSEELVDAAVKMADLSPEDPFISVSDQQGTYTNTASDLMIFDDSIVEMDS
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCEEEECCCHHHHHH
IRDILQEMEGEALSVDSRIVNSEGASFSKSLTETALMTTRGFCGSYKKTYFSSSISVVAA
HHHHHHHHCCCEEECCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEEEE
SDDEKMEVDYSFAVKSKFSDLEKSRVLGREAANRALRKLNARELQTCKMPVLFENRVASS
CCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHH
LLSNFASAINGASIADKTSFLLNDLGTNVFGSDIYIVDDPLMVSGISSRPFDGEGILSTK
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCEEECCCCCCCCCCCHHHHH
RSIVERGVLKSWILDMRTAKQLALSTTGHAVRKDNASVSPGVSNFYIQRSDITVQELMSD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCEEEEECCCCHHHHHHH
IKRGLYITDLFGFGINFATGDYSQGAFGYMIENGDITYPVHGITIAGNLRDMFSSAQVAN
HHCCEEEEEHHHCCCEECCCCCCCCCEEEEEECCCEEEEEEEEEEECCHHHHHHHHHHHH
DLIFLRSVNSPTLRFGDMVVSGA
HEEEEEECCCCCEEECCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]