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The map label for this gene is folE [H]

Identifier: 88606705

GI number: 88606705

Start: 727300

End: 727908

Strand: Reverse

Name: folE [H]

Synonym: APH_0706

Alternate gene names: 88606705

Gene position: 727908-727300 (Counterclockwise)

Preceding gene: 88607413

Following gene: 88607884

Centisome position: 49.47

GC content: 41.05

Gene sequence:

>609_bases
ATGGCAGAAGTAACGCAATGTGGTAGAGTTTGTCCAACGATTGAAGAAGCTGAAGAGGCGGTTAGAGTCATAATAAGGTG
GATGGGGGATGATCCTAATAGGGAGGGTTTATTAAAAACGCCTCGTAGGGTCTTGGATTACTATAAAAAGTCTTGTTCTG
GTTATGCTCCTGAGCTTCTCTCTCACTATTCTATTCTTCCCTATGATGGGAGTAGTTCAGAAATGGTGTTGGTGAAGAAC
ATAGGGTTTTCTTCAAACTGCGAACATCATCTTAGTCCTATATGTGGTGTGGCGCATATCGCTTATATGCCAAAAGAGAA
GATACTTGGTATTGGTAGTATAGCGCATATTGTTGGTTGTTTTTCGAAAAGGCTACAGCTTCAGGAGCGTCTTACTAATC
AGGTGGCAGAGTATTTAGAGAAGATACTAGATCCTCATGGTGTTGCTGTACTACTAGAGGCCGAGCATTGGTGCGTTGAA
TGTAGCAACAGGGATACTGCCTCTAGAGATGAGAGGTTGAATGTACAAACTAGTCAAATGTTAGGGGTTTTTGAAGAGCG
TTGTGATTTGCGCAATGAATTCTTCTTAAGAATTAAAATGTATAAGTAG

Upstream 100 bases:

>100_bases
CTAAACAAGTTGGTCTTAGATTACAATCGATCAAGACAGGCGGCAATAACCACGGATCTAATAGAAGTGGTTAGTGGATG
CGAAGCTTTGGGATAGGTAT

Downstream 100 bases:

>100_bases
AGGTGTGTCTTGAGTGCCCTGAATATAGCAGAAGATATAATTAAACTTATAAAGTCTCGGGGAGTTGAGGGAGAAGTCGT
ATTAACTCAGCACGAGACTA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MAEVTQCGRVCPTIEEAEEAVRVIIRWMGDDPNREGLLKTPRRVLDYYKKSCSGYAPELLSHYSILPYDGSSSEMVLVKN
IGFSSNCEHHLSPICGVAHIAYMPKEKILGIGSIAHIVGCFSKRLQLQERLTNQVAEYLEKILDPHGVAVLLEAEHWCVE
CSNRDTASRDERLNVQTSQMLGVFEERCDLRNEFFLRIKMYK

Sequences:

>Translated_202_residues
MAEVTQCGRVCPTIEEAEEAVRVIIRWMGDDPNREGLLKTPRRVLDYYKKSCSGYAPELLSHYSILPYDGSSSEMVLVKN
IGFSSNCEHHLSPICGVAHIAYMPKEKILGIGSIAHIVGCFSKRLQLQERLTNQVAEYLEKILDPHGVAVLLEAEHWCVE
CSNRDTASRDERLNVQTSQMLGVFEERCDLRNEFFLRIKMYK
>Mature_201_residues
AEVTQCGRVCPTIEEAEEAVRVIIRWMGDDPNREGLLKTPRRVLDYYKKSCSGYAPELLSHYSILPYDGSSSEMVLVKNI
GFSSNCEHHLSPICGVAHIAYMPKEKILGIGSIAHIVGCFSKRLQLQERLTNQVAEYLEKILDPHGVAVLLEAEHWCVEC
SNRDTASRDERLNVQTSQMLGVFEERCDLRNEFFLRIKMYK

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=181, Percent_Identity=39.7790055248619, Blast_Score=129, Evalue=1e-30,
Organism=Homo sapiens, GI4503949, Length=181, Percent_Identity=39.7790055248619, Blast_Score=129, Evalue=1e-30,
Organism=Homo sapiens, GI66932972, Length=131, Percent_Identity=41.9847328244275, Blast_Score=111, Evalue=5e-25,
Organism=Homo sapiens, GI66932970, Length=135, Percent_Identity=41.4814814814815, Blast_Score=110, Evalue=6e-25,
Organism=Escherichia coli, GI1788476, Length=177, Percent_Identity=31.638418079096, Blast_Score=98, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17560486, Length=178, Percent_Identity=41.0112359550562, Blast_Score=136, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6321706, Length=184, Percent_Identity=38.0434782608696, Blast_Score=119, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24656782, Length=176, Percent_Identity=39.2045454545455, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24656791, Length=176, Percent_Identity=39.2045454545455, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24656796, Length=176, Percent_Identity=39.2045454545455, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 23024; Mature: 22893

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEVTQCGRVCPTIEEAEEAVRVIIRWMGDDPNREGLLKTPRRVLDYYKKSCSGYAPELL
CCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHH
SHYSILPYDGSSSEMVLVKNIGFSSNCEHHLSPICGVAHIAYMPKEKILGIGSIAHIVGC
HHCEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHH
FSKRLQLQERLTNQVAEYLEKILDPHGVAVLLEAEHWCVECSNRDTASRDERLNVQTSQM
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCCCCCCHHCCCHHHHH
LGVFEERCDLRNEFFLRIKMYK
HHHHHHHHCCCCEEEEEEEECC
>Mature Secondary Structure 
AEVTQCGRVCPTIEEAEEAVRVIIRWMGDDPNREGLLKTPRRVLDYYKKSCSGYAPELL
CCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHH
SHYSILPYDGSSSEMVLVKNIGFSSNCEHHLSPICGVAHIAYMPKEKILGIGSIAHIVGC
HHCEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHH
FSKRLQLQERLTNQVAEYLEKILDPHGVAVLLEAEHWCVECSNRDTASRDERLNVQTSQM
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCCCCCCHHCCCHHHHH
LGVFEERCDLRNEFFLRIKMYK
HHHHHHHHCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA