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The map label for this gene is ppdK [H]

Identifier: 88607802

GI number: 88607802

Start: 184143

End: 186767

Strand: Reverse

Name: ppdK [H]

Synonym: APH_0185

Alternate gene names: 88607802

Gene position: 186767-184143 (Counterclockwise)

Preceding gene: 88607114

Following gene: 88606827

Centisome position: 12.69

GC content: 46.13

Gene sequence:

>2625_bases
ATGGATGATAGGCTGATTTACCACTTCGGAAGAAGTAGGTGTGATGGTGGAGCACATTTGGCGCACTTGTTAGGTGGGAA
AGGAGCAGGCCTTGCGGAAATGTGTAGGATTGGGATTAATGTTCCCCCTGGGTTTACCATAGCCACATCAGTGTGCAATC
TATATCAGGAAAGTGGCAGTGTTCCAGAAAATGTAGTGCAACAACTCCCTGAAGCGTTGAGCCTCCTGGGACAAGAAGTG
GATCTAGAATTTGGGAATCCGGACCGTCCGTTGTTAGTATCGGTACGCTCAGGAAGTGTCCAATCTATGCCTGGAATGCT
AGATACCGTACTCAATGTTGGGTTAAACGACGAAGTAGCAGTAAAGTTGGGAGCTATGCGCGGAGGTAGGTTTGCCTATG
ACAGCTATAGGCGGCTTATACAGATGTATGCTGCTTCTGTTTTGCAGTTGGAAGATAGGATTTTCGAAGAGCGTTATAAA
GAAAAACAGAAAGAACTGTCTCTGTCGACAGGGGAATCTATAACAAATCAAGAAGCTTTGCGAGAATTGGTTGAAGAGTT
CAAACAATTAGTACGTACTCATACGGGACAGGAATTCCCCCAAGATGTTCAGGTTCAGCTCCGTAATGCAATAGGTGCGG
TGTTCAAATCATGGATGAATCAGCGTGCTGTAGCATATAGAAACATGTACGGCATACCCTCAGACGCAGGTACTGCTGTG
AATGTGCAGGCAATGGTATTCGGAAATATAAATCAAAATTCGGCCACAGGCGTGGTATTCACTCGTAATCCTTCTACAGG
GGAGAAGGAAATATTTGGCGAATTTCTATGCAATGCCCAAGGTGAAGATATAGTTTCCGGGCAGAAGGATCCGTCTCCTA
TTAAGCTCATGGAAAGTTCTATGCCTCAGGTATACGGGGAGCTTGTGGAAGTATGTGAAAAGCTCGAGAAACATAACAAA
GACATGCAGGATATAGAGTTTACTGTCCAAGATGGTAAACTGTGGATTCTACAGACTAGACGCGGTAAACGCAGCGCTCA
TGCGGCCGTGAACTTGGCTGTTAGTATGGTGAAAGAGTCGCTTATTTCCAAGGAGGAGGCTATACTTCGTATTGATGCTA
GCACCCTTGGGGGGCTTTTCCATCCAATACTAGAAGAAAACTGTAGCAATGTAGTAGTGGGTAGAGGGCTGCCAGCATCC
CCTGGTGCGGCGTCCGGCTACGTAGCTTTTACATCGAGTGATGCAGAGGAGCTTAAGCGTCAGGGTAAGAGTGTGATACT
GGTCCGCCAGGAGACAAGCCCTGAGGATATTCGTGGTATGATTTCCGCTGCTGGGGTTTTGACTCTGCGCGGAGGGATGA
CTTCCCACGCCGCCGTTGTTGCACGTGGTATGGGTAAGCCGTGCATATGTGGGGCTAGTAGTTTGTGTATAGACGAGAGT
GGGCAGTTTTTCTTCAACGAGGATAATGTAAAAATCGGTCGTTCAGACAGCATAACCATAAATGGCAGTACTGGTGAGGT
AATGCTGGGAGCCGTGCAAACTGTGACCCCTTCTCTGCCGGAAGCTTTCTATGAATTGATGGGTTGGATAGATGAAATCA
GGACAGTAGGGGTCATGGCAAATGCTGATACTGTGGAGGATATGGGCACTGCTAATCTTTTTGGTGCCGATGGTATTGGG
CTATGCAGAACTGAGCACATGTTCTTCTCTGATAACAGAATCACGATAGTGCGTGAAATGATTCTTGCGAGCAATAAGCA
AGAGCGAGACCGGGCTCTTAAGAAGATTGAAGTTGTGCAGAAGGACGACTTCCTGGCTATGTTCAAGCTCATGCAGAATA
AGAAGCAGATTACCGTTAGGCTACTTGATCCACCGTTGCATGAGTTTTTACCTAATTCTATGGAGGCTTTGGAGAGTATC
TCGTTAGGAATGGGGCGTTCTTTGGAATACATAAAGCACAGGGTTTCGGCGCTTTCTGAAAAGAATCCTATGCTTGGTCA
TAGGGGTTGCAGGCTTGCTGTCTCTTATCCGGAGATATATGAAATGCAAATTCGTGCAATATTCCAAGCAGTGAAAGAGC
TTCGTGATTCAGATGGCATTACTGTTATTCCGGAAATCATGATTCCGTTTGTTATGGACGAACAGGAGATAGTGATTATC
GGTGACTTAGTAAAGTGCATTTCAGCGCAGTTTGATAATGCACAGTATAGGATTGGTGCCATGATTGAGCTACCTAGGGC
AGCATTATTGGCTGACAAGTTATCTCAGCACGTGCAGTTTTTCAGTTTCGGCACGAATGACCTAACGCAAACTACCCTTG
GGATCTCGCGAGATGATTCATCTAAGTTTGTTGAGCACTATATAAGTTCCGGTGTTTTTGCTTCTGATCCTTTCGAGCTC
TTGGATGTAAGTGGCGTTGGAAGGTTGATCAAGATTGCGATGGAGCTGATACATAAATCGCCGAACAAAGTCAAAACCGG
GCTATGCGGCGAGCATGGTGGCACTTTAGCGGCAATGCAGCTGTGCTCAGAATTGGATATAGATTATGTTTCCTGTTCAC
CATATAAAGTGCCTGTGGCAAAACTGATCGCGGCACAGTGTGCAATACGGAACAAGATGCTCTAA

Upstream 100 bases:

>100_bases
CTGTATAGTTGCGCTATGAGGTATTAGGCTTTATATTGTCCATATGGGAGTATCACTGTATATTTCTCTATCTCATCTTT
GAAAGTCTGGTAAAAAACAT

Downstream 100 bases:

>100_bases
CTTACACTTTGTAGTGTTGTATTAGTTGATCGCGTCCAGGTGCTGTAATATTCCCCAGGTAGTTCATGGTGGGCCGGTGG
TCATATAGGTGTACTGGACG

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 874; Mature: 874

Protein sequence:

>874_residues
MDDRLIYHFGRSRCDGGAHLAHLLGGKGAGLAEMCRIGINVPPGFTIATSVCNLYQESGSVPENVVQQLPEALSLLGQEV
DLEFGNPDRPLLVSVRSGSVQSMPGMLDTVLNVGLNDEVAVKLGAMRGGRFAYDSYRRLIQMYAASVLQLEDRIFEERYK
EKQKELSLSTGESITNQEALRELVEEFKQLVRTHTGQEFPQDVQVQLRNAIGAVFKSWMNQRAVAYRNMYGIPSDAGTAV
NVQAMVFGNINQNSATGVVFTRNPSTGEKEIFGEFLCNAQGEDIVSGQKDPSPIKLMESSMPQVYGELVEVCEKLEKHNK
DMQDIEFTVQDGKLWILQTRRGKRSAHAAVNLAVSMVKESLISKEEAILRIDASTLGGLFHPILEENCSNVVVGRGLPAS
PGAASGYVAFTSSDAEELKRQGKSVILVRQETSPEDIRGMISAAGVLTLRGGMTSHAAVVARGMGKPCICGASSLCIDES
GQFFFNEDNVKIGRSDSITINGSTGEVMLGAVQTVTPSLPEAFYELMGWIDEIRTVGVMANADTVEDMGTANLFGADGIG
LCRTEHMFFSDNRITIVREMILASNKQERDRALKKIEVVQKDDFLAMFKLMQNKKQITVRLLDPPLHEFLPNSMEALESI
SLGMGRSLEYIKHRVSALSEKNPMLGHRGCRLAVSYPEIYEMQIRAIFQAVKELRDSDGITVIPEIMIPFVMDEQEIVII
GDLVKCISAQFDNAQYRIGAMIELPRAALLADKLSQHVQFFSFGTNDLTQTTLGISRDDSSKFVEHYISSGVFASDPFEL
LDVSGVGRLIKIAMELIHKSPNKVKTGLCGEHGGTLAAMQLCSELDIDYVSCSPYKVPVAKLIAAQCAIRNKML

Sequences:

>Translated_874_residues
MDDRLIYHFGRSRCDGGAHLAHLLGGKGAGLAEMCRIGINVPPGFTIATSVCNLYQESGSVPENVVQQLPEALSLLGQEV
DLEFGNPDRPLLVSVRSGSVQSMPGMLDTVLNVGLNDEVAVKLGAMRGGRFAYDSYRRLIQMYAASVLQLEDRIFEERYK
EKQKELSLSTGESITNQEALRELVEEFKQLVRTHTGQEFPQDVQVQLRNAIGAVFKSWMNQRAVAYRNMYGIPSDAGTAV
NVQAMVFGNINQNSATGVVFTRNPSTGEKEIFGEFLCNAQGEDIVSGQKDPSPIKLMESSMPQVYGELVEVCEKLEKHNK
DMQDIEFTVQDGKLWILQTRRGKRSAHAAVNLAVSMVKESLISKEEAILRIDASTLGGLFHPILEENCSNVVVGRGLPAS
PGAASGYVAFTSSDAEELKRQGKSVILVRQETSPEDIRGMISAAGVLTLRGGMTSHAAVVARGMGKPCICGASSLCIDES
GQFFFNEDNVKIGRSDSITINGSTGEVMLGAVQTVTPSLPEAFYELMGWIDEIRTVGVMANADTVEDMGTANLFGADGIG
LCRTEHMFFSDNRITIVREMILASNKQERDRALKKIEVVQKDDFLAMFKLMQNKKQITVRLLDPPLHEFLPNSMEALESI
SLGMGRSLEYIKHRVSALSEKNPMLGHRGCRLAVSYPEIYEMQIRAIFQAVKELRDSDGITVIPEIMIPFVMDEQEIVII
GDLVKCISAQFDNAQYRIGAMIELPRAALLADKLSQHVQFFSFGTNDLTQTTLGISRDDSSKFVEHYISSGVFASDPFEL
LDVSGVGRLIKIAMELIHKSPNKVKTGLCGEHGGTLAAMQLCSELDIDYVSCSPYKVPVAKLIAAQCAIRNKML
>Mature_874_residues
MDDRLIYHFGRSRCDGGAHLAHLLGGKGAGLAEMCRIGINVPPGFTIATSVCNLYQESGSVPENVVQQLPEALSLLGQEV
DLEFGNPDRPLLVSVRSGSVQSMPGMLDTVLNVGLNDEVAVKLGAMRGGRFAYDSYRRLIQMYAASVLQLEDRIFEERYK
EKQKELSLSTGESITNQEALRELVEEFKQLVRTHTGQEFPQDVQVQLRNAIGAVFKSWMNQRAVAYRNMYGIPSDAGTAV
NVQAMVFGNINQNSATGVVFTRNPSTGEKEIFGEFLCNAQGEDIVSGQKDPSPIKLMESSMPQVYGELVEVCEKLEKHNK
DMQDIEFTVQDGKLWILQTRRGKRSAHAAVNLAVSMVKESLISKEEAILRIDASTLGGLFHPILEENCSNVVVGRGLPAS
PGAASGYVAFTSSDAEELKRQGKSVILVRQETSPEDIRGMISAAGVLTLRGGMTSHAAVVARGMGKPCICGASSLCIDES
GQFFFNEDNVKIGRSDSITINGSTGEVMLGAVQTVTPSLPEAFYELMGWIDEIRTVGVMANADTVEDMGTANLFGADGIG
LCRTEHMFFSDNRITIVREMILASNKQERDRALKKIEVVQKDDFLAMFKLMQNKKQITVRLLDPPLHEFLPNSMEALESI
SLGMGRSLEYIKHRVSALSEKNPMLGHRGCRLAVSYPEIYEMQIRAIFQAVKELRDSDGITVIPEIMIPFVMDEQEIVII
GDLVKCISAQFDNAQYRIGAMIELPRAALLADKLSQHVQFFSFGTNDLTQTTLGISRDDSSKFVEHYISSGVFASDPFEL
LDVSGVGRLIKIAMELIHKSPNKVKTGLCGEHGGTLAAMQLCSELDIDYVSCSPYKVPVAKLIAAQCAIRNKML

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=468, Percent_Identity=26.7094017094017, Blast_Score=103, Evalue=6e-23,
Organism=Escherichia coli, GI1787994, Length=696, Percent_Identity=23.9942528735632, Blast_Score=98, Evalue=3e-21,
Organism=Escherichia coli, GI48994992, Length=442, Percent_Identity=24.4343891402715, Blast_Score=94, Evalue=4e-20,
Organism=Escherichia coli, GI1789193, Length=356, Percent_Identity=25.8426966292135, Blast_Score=88, Evalue=3e-18,
Organism=Escherichia coli, GI1788726, Length=457, Percent_Identity=22.7571115973742, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 95915; Mature: 95915

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDRLIYHFGRSRCDGGAHLAHLLGGKGAGLAEMCRIGINVPPGFTIATSVCNLYQESGS
CCCCEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
VPENVVQQLPEALSLLGQEVDLEFGNPDRPLLVSVRSGSVQSMPGMLDTVLNVGLNDEVA
CHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCEE
VKLGAMRGGRFAYDSYRRLIQMYAASVLQLEDRIFEERYKEKQKELSLSTGESITNQEAL
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RELVEEFKQLVRTHTGQEFPQDVQVQLRNAIGAVFKSWMNQRAVAYRNMYGIPSDAGTAV
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
NVQAMVFGNINQNSATGVVFTRNPSTGEKEIFGEFLCNAQGEDIVSGQKDPSPIKLMESS
EEEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
MPQVYGELVEVCEKLEKHNKDMQDIEFTVQDGKLWILQTRRGKRSAHAAVNLAVSMVKES
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHH
LISKEEAILRIDASTLGGLFHPILEENCSNVVVGRGLPASPGAASGYVAFTSSDAEELKR
HHCCCHHEEEECHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCEEEEECCCHHHHHH
QGKSVILVRQETSPEDIRGMISAAGVLTLRGGMTSHAAVVARGMGKPCICGASSLCIDES
CCCEEEEEECCCCHHHHHHHHHHCCEEEECCCCCHHHHHHHHCCCCCCCCCCCCEEEECC
GQFFFNEDNVKIGRSDSITINGSTGEVMLGAVQTVTPSLPEAFYELMGWIDEIRTVGVMA
CCEEEECCCEEECCCCEEEEECCCCCEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHEEE
NADTVEDMGTANLFGADGIGLCRTEHMFFSDNRITIVREMILASNKQERDRALKKIEVVQ
CCCCHHHCCCCCCCCCCCCCEEEECCEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHC
KDDFLAMFKLMQNKKQITVRLLDPPLHEFLPNSMEALESISLGMGRSLEYIKHRVSALSE
CCHHHHHHHHHCCCCEEEEEEECCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHC
KNPMLGHRGCRLAVSYPEIYEMQIRAIFQAVKELRDSDGITVIPEIMIPFVMDEQEIVII
CCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHEECCCCEEEE
GDLVKCISAQFDNAQYRIGAMIELPRAALLADKLSQHVQFFSFGTNDLTQTTLGISRDDS
HHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCH
SKFVEHYISSGVFASDPFELLDVSGVGRLIKIAMELIHKSPNKVKTGLCGEHGGTLAAMQ
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHHHHH
LCSELDIDYVSCSPYKVPVAKLIAAQCAIRNKML
HHHHCCCCEECCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDDRLIYHFGRSRCDGGAHLAHLLGGKGAGLAEMCRIGINVPPGFTIATSVCNLYQESGS
CCCCEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
VPENVVQQLPEALSLLGQEVDLEFGNPDRPLLVSVRSGSVQSMPGMLDTVLNVGLNDEVA
CHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCEE
VKLGAMRGGRFAYDSYRRLIQMYAASVLQLEDRIFEERYKEKQKELSLSTGESITNQEAL
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RELVEEFKQLVRTHTGQEFPQDVQVQLRNAIGAVFKSWMNQRAVAYRNMYGIPSDAGTAV
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
NVQAMVFGNINQNSATGVVFTRNPSTGEKEIFGEFLCNAQGEDIVSGQKDPSPIKLMESS
EEEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
MPQVYGELVEVCEKLEKHNKDMQDIEFTVQDGKLWILQTRRGKRSAHAAVNLAVSMVKES
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHH
LISKEEAILRIDASTLGGLFHPILEENCSNVVVGRGLPASPGAASGYVAFTSSDAEELKR
HHCCCHHEEEECHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCEEEEECCCHHHHHH
QGKSVILVRQETSPEDIRGMISAAGVLTLRGGMTSHAAVVARGMGKPCICGASSLCIDES
CCCEEEEEECCCCHHHHHHHHHHCCEEEECCCCCHHHHHHHHCCCCCCCCCCCCEEEECC
GQFFFNEDNVKIGRSDSITINGSTGEVMLGAVQTVTPSLPEAFYELMGWIDEIRTVGVMA
CCEEEECCCEEECCCCEEEEECCCCCEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHEEE
NADTVEDMGTANLFGADGIGLCRTEHMFFSDNRITIVREMILASNKQERDRALKKIEVVQ
CCCCHHHCCCCCCCCCCCCCEEEECCEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHC
KDDFLAMFKLMQNKKQITVRLLDPPLHEFLPNSMEALESISLGMGRSLEYIKHRVSALSE
CCHHHHHHHHHCCCCEEEEEEECCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHC
KNPMLGHRGCRLAVSYPEIYEMQIRAIFQAVKELRDSDGITVIPEIMIPFVMDEQEIVII
CCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHEECCCCEEEE
GDLVKCISAQFDNAQYRIGAMIELPRAALLADKLSQHVQFFSFGTNDLTQTTLGISRDDS
HHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCH
SKFVEHYISSGVFASDPFELLDVSGVGRLIKIAMELIHKSPNKVKTGLCGEHGGTLAAMQ
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHHHHH
LCSELDIDYVSCSPYKVPVAKLIAAQCAIRNKML
HHHHCCCCEECCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 9168612 [H]