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The map label for this gene is nuoB

Identifier: 88607775

GI number: 88607775

Start: 540782

End: 541306

Strand: Direct

Name: nuoB

Synonym: APH_0520

Alternate gene names: 88607775

Gene position: 540782-541306 (Clockwise)

Preceding gene: 88607210

Following gene: 88606931

Centisome position: 36.76

GC content: 47.43

Gene sequence:

>525_bases
ATGGGTGAATGTGATCAGATACTAGAAAATGATCTATGGCAGGGCTATAAGGATCAGGGTTTTGTGGTAACGCAATTCTC
GGACCTAATAGAGAGAGTGCTGAAATGGGCGCGCAGTGGGTCTTTGTGGCCTATGACTTTTGGCCTGGCTTGTTGTGCGG
TTGAGATGATGCACACTGCTGCTAGTAGATATGACCTAGATAGATACGGGATAATGTTCAGGGCCAGTCCAAGGCAGGCT
GATGTTATGATAGTGGCTGGTACGCTTACTAATAAAATGGCGCCCGCGTTACGTCGTGTATATGACCAGATGGCAGACCC
TAAGTATGTTATTTCAATGGGTAGTTGTGCAAATGGGGGTGGCTATTATCACTACTCGTACTCTGTTGTTAGGGGCTGTG
ATAGGGTAGTTCCTGTAGATATATATGTGCCTGGATGCCCTCCGACAGCGGAAGCCTTGTTATATGGTTTGCTGTGTTTA
CAGCAGAAGATCCTTCGCAACGGTCAAGGAACTTGGAGGGCGTAG

Upstream 100 bases:

>100_bases
TTGGTTTTGCGGGGTATTTTTCGATGCTATGTTTCCTGGGAATGCTAACTATCGGTTTTATATACGAATGGAATAGTGGT
GCCTTAGAATGGGAGTAGGC

Downstream 100 bases:

>100_bases
AATGTCTTTAGATATTGAGCCTGTTGCTAGCTCGCATATACAGGAACTGTTGCAGACTGATATTGTGTTGCGCCAAGATG
GTGTGCTGCAGTGTTATCTT

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 174; Mature: 173

Protein sequence:

>174_residues
MGECDQILENDLWQGYKDQGFVVTQFSDLIERVLKWARSGSLWPMTFGLACCAVEMMHTAASRYDLDRYGIMFRASPRQA
DVMIVAGTLTNKMAPALRRVYDQMADPKYVISMGSCANGGGYYHYSYSVVRGCDRVVPVDIYVPGCPPTAEALLYGLLCL
QQKILRNGQGTWRA

Sequences:

>Translated_174_residues
MGECDQILENDLWQGYKDQGFVVTQFSDLIERVLKWARSGSLWPMTFGLACCAVEMMHTAASRYDLDRYGIMFRASPRQA
DVMIVAGTLTNKMAPALRRVYDQMADPKYVISMGSCANGGGYYHYSYSVVRGCDRVVPVDIYVPGCPPTAEALLYGLLCL
QQKILRNGQGTWRA
>Mature_173_residues
GECDQILENDLWQGYKDQGFVVTQFSDLIERVLKWARSGSLWPMTFGLACCAVEMMHTAASRYDLDRYGIMFRASPRQAD
VMIVAGTLTNKMAPALRRVYDQMADPKYVISMGSCANGGGYYHYSYSVVRGCDRVVPVDIYVPGCPPTAEALLYGLLCLQ
QKILRNGQGTWRA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=147, Percent_Identity=78.2312925170068, Blast_Score=251, Evalue=3e-67,
Organism=Escherichia coli, GI1788624, Length=140, Percent_Identity=52.1428571428571, Blast_Score=160, Evalue=5e-41,
Organism=Escherichia coli, GI1789074, Length=127, Percent_Identity=42.5196850393701, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI1788834, Length=121, Percent_Identity=39.6694214876033, Blast_Score=99, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17509685, Length=141, Percent_Identity=75.886524822695, Blast_Score=236, Evalue=3e-63,
Organism=Drosophila melanogaster, GI18859983, Length=165, Percent_Identity=68.4848484848485, Blast_Score=247, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24642371, Length=165, Percent_Identity=68.4848484848485, Blast_Score=247, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24651058, Length=137, Percent_Identity=79.5620437956204, Blast_Score=243, Evalue=5e-65,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_ANAPZ (Q2GKI6)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505115.1
- ProteinModelPortal:   Q2GKI6
- SMR:   Q2GKI6
- STRING:   Q2GKI6
- GeneID:   3930812
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0520
- NMPDR:   fig|212042.5.peg.510
- TIGR:   APH_0520
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   FIATSMS
- PhylomeDB:   Q2GKI6
- ProtClustDB:   PRK06411
- BioCyc:   APHA212042:APH_0520-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 19485; Mature: 19354

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
9.2 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
8.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGECDQILENDLWQGYKDQGFVVTQFSDLIERVLKWARSGSLWPMTFGLACCAVEMMHTA
CCHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
ASRYDLDRYGIMFRASPRQADVMIVAGTLTNKMAPALRRVYDQMADPKYVISMGSCANGG
HHHCCHHHHCEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
GYYHYSYSVVRGCDRVVPVDIYVPGCPPTAEALLYGLLCLQQKILRNGQGTWRA
CEEEEHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GECDQILENDLWQGYKDQGFVVTQFSDLIERVLKWARSGSLWPMTFGLACCAVEMMHTA
CHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
ASRYDLDRYGIMFRASPRQADVMIVAGTLTNKMAPALRRVYDQMADPKYVISMGSCANGG
HHHCCHHHHCEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
GYYHYSYSVVRGCDRVVPVDIYVPGCPPTAEALLYGLLCLQQKILRNGQGTWRA
CEEEEHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA