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The map label for this gene is prfB [H]

Identifier: 88607753

GI number: 88607753

Start: 495029

End: 495709

Strand: Direct

Name: prfB [H]

Synonym: APH_0468

Alternate gene names: 88607753

Gene position: 495029-495709 (Clockwise)

Preceding gene: 88607411

Following gene: 88607025

Centisome position: 33.65

GC content: 43.17

Gene sequence:

>681_bases
GTGGAGGATGACGCTGAAAAAGCAAAAAGCTTGCTTAGTGAACGGTCGAGAGTGAGCGCCGTTCTCACATCTTTTCGTGT
TTTAGAAAAAGAACATAAGGACTTAGCTGAACTTGCAGAGATGATTTCTGACAGTGATGACAGAGACTTTCTTGCAACGA
TAAATGCTGAGCTTCAATCCTTGAAGTTGCGTATCTCTCAGAAAAAGACTGAGTGCATGTTCTCTCACGAAGCTGATGCT
AATAGTTGTTTCTTGACTATTCGTTCTGGAGCTGGTGGAACCGAAAGTAGCGATTGGGTTTGCATGCTTTTACGCATGTA
TACAAAGTGGGCTGAATCTTTTCATGAGTTTACTGTTGAGACTGTTGATTCTGTTGACGGTGAGGAAACAGGTCTGAAGG
CTGTTACTGTGAAAATATGTGGTATTGGCGCCTACGGGTGGGCACGTACTGAAAGTGGTATTCATAGGTTAGTTCGCATT
TCTCCCTTTGACCATGCTGCAAAACGGCACACCAGCTTCGCTAGTGTTGAGGTATCTCCTGTTGTGGATCATGAAATTGA
CATAAAAATTCTAGAAAAGGATCTTCGTATTGATACTTATCGTGCTTCTGGTGCTGGGGGGCAGCATGTTAATAAAACAG
AAAGTGCGGTCCGTAAGTTTAGCTGTTATCCCCACTTTTAG

Upstream 100 bases:

>100_bases
AAAATAACATTAGATATTTCAATCCCTTCTAAGCAATACCATTTTTTTATAGCCTAGCGTCCAGCTACTGAACTCTCCTT
TATTTTACATCTACTTTTTA

Downstream 100 bases:

>100_bases
CTCATATTAAGTGTTATTTGAATGTTACTTTGGCTAATATTTTTTGGGGTAAGGGGCTTGTTTTTCGTAATTTTAAAGAG
TATACACTTAATATGTGGCA

Product: peptide chain release factor 2, truncation

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MEDDAEKAKSLLSERSRVSAVLTSFRVLEKEHKDLAELAEMISDSDDRDFLATINAELQSLKLRISQKKTECMFSHEADA
NSCFLTIRSGAGGTESSDWVCMLLRMYTKWAESFHEFTVETVDSVDGEETGLKAVTVKICGIGAYGWARTESGIHRLVRI
SPFDHAAKRHTSFASVEVSPVVDHEIDIKILEKDLRIDTYRASGAGGQHVNKTESAVRKFSCYPHF

Sequences:

>Translated_226_residues
MEDDAEKAKSLLSERSRVSAVLTSFRVLEKEHKDLAELAEMISDSDDRDFLATINAELQSLKLRISQKKTECMFSHEADA
NSCFLTIRSGAGGTESSDWVCMLLRMYTKWAESFHEFTVETVDSVDGEETGLKAVTVKICGIGAYGWARTESGIHRLVRI
SPFDHAAKRHTSFASVEVSPVVDHEIDIKILEKDLRIDTYRASGAGGQHVNKTESAVRKFSCYPHF
>Mature_226_residues
MEDDAEKAKSLLSERSRVSAVLTSFRVLEKEHKDLAELAEMISDSDDRDFLATINAELQSLKLRISQKKTECMFSHEADA
NSCFLTIRSGAGGTESSDWVCMLLRMYTKWAESFHEFTVETVDSVDGEETGLKAVTVKICGIGAYGWARTESGIHRLVRI
SPFDHAAKRHTSFASVEVSPVVDHEIDIKILEKDLRIDTYRASGAGGQHVNKTESAVRKFSCYPHF

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=206, Percent_Identity=33.495145631068, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI166795305, Length=206, Percent_Identity=33.495145631068, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI34577120, Length=215, Percent_Identity=30.2325581395349, Blast_Score=94, Evalue=7e-20,
Organism=Escherichia coli, GI2367172, Length=216, Percent_Identity=49.0740740740741, Blast_Score=215, Evalue=2e-57,
Organism=Escherichia coli, GI1787462, Length=225, Percent_Identity=34.2222222222222, Blast_Score=114, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17542784, Length=189, Percent_Identity=29.6296296296296, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321295, Length=213, Percent_Identity=30.9859154929577, Blast_Score=81, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19921226, Length=218, Percent_Identity=33.9449541284404, Blast_Score=101, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 25267; Mature: 25267

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDDAEKAKSLLSERSRVSAVLTSFRVLEKEHKDLAELAEMISDSDDRDFLATINAELQS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LKLRISQKKTECMFSHEADANSCFLTIRSGAGGTESSDWVCMLLRMYTKWAESFHEFTVE
HHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TVDSVDGEETGLKAVTVKICGIGAYGWARTESGIHRLVRISPFDHAAKRHTSFASVEVSP
HHHCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHEEECCCHHHHHHCCCHHEEEECC
VVDHEIDIKILEKDLRIDTYRASGAGGQHVNKTESAVRKFSCYPHF
CCCCCCCCEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MEDDAEKAKSLLSERSRVSAVLTSFRVLEKEHKDLAELAEMISDSDDRDFLATINAELQS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LKLRISQKKTECMFSHEADANSCFLTIRSGAGGTESSDWVCMLLRMYTKWAESFHEFTVE
HHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TVDSVDGEETGLKAVTVKICGIGAYGWARTESGIHRLVRISPFDHAAKRHTSFASVEVSP
HHHCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHEEECCCHHHHHHCCCHHEEEECC
VVDHEIDIKILEKDLRIDTYRASGAGGQHVNKTESAVRKFSCYPHF
CCCCCCCCEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA