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The map label for this gene is pyrF [H]

Identifier: 88607476

GI number: 88607476

Start: 546629

End: 547315

Strand: Reverse

Name: pyrF [H]

Synonym: APH_0527

Alternate gene names: 88607476

Gene position: 547315-546629 (Counterclockwise)

Preceding gene: 88606752

Following gene: 88607474

Centisome position: 37.2

GC content: 42.36

Gene sequence:

>687_bases
ATGCATCAGAAGAATCCAATAATTTGCGCTCTTGATACAGCAGATCTAGAACGTGCAACTACCTTAGCAAAGGCAATATC
TGGTAAGGTTGCTATGGCAAAGGTAGGGCTAGAATTTTTTACAGCTCACGGACTTTCCGGTGTACAGAAAATTGTTGATC
TAGGATTTAGAATATTTCTAGACCTCAAACTACACGATATTCCTAATACAGTCCGTGGTGCGTTAAAATCCGTGGTAAAC
CTTCCTGCTATCGATATGTTGACCATACATGTTAGCGGTGGGAAAAACATGATTATGGGCGCTATCGATGCTCTTAAGGA
ATGCGATATAGAACCTGTCGGGGTGACTGTCCTCACTAGTATGGACGATGTTGATCTTCGAGCTTGTGGGATAAATTCCT
CAGTTGAAGCACACGTTATGAATCTAGTAGATCTTGCCGTGAGCTCAGGTCTTAACGCAATAGTTTGCTCTGCAAAAGAA
GTGGCTAGTATACGTAAAAAGTATCCATCTTTAAAGCTTGTAGTACCGGGCATAAGGATGGCTCAATCTGCAACAGGAGA
CCAAAAAAGAGTTGATACTCCTAAAGATACACTTGCAGCTGGAGCTGACTACCTTGTTATAGGCCGTGTGATTACACAGA
GTTCAGATCCTGCCGGTGAGATAGACAAGATCTTAAAGACGCTTTAG

Upstream 100 bases:

>100_bases
GAAAGTTTGGCTCAGATATGGGAAGAAAGCGTTGACGTAGTTAGCGAAGTAACTACGAAGAATTTTTTCACACTTTTTGA
TAAATGTGCTCGTCTATACT

Downstream 100 bases:

>100_bases
AACCCTTTTGCACAAGTCATTGCGTCTATGTACGTAGAATCAGATTGAACATATCCGGTGGTTTATAGCGTAAATGCGGT
CACCGCTGCATCACATTTGT

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MHQKNPIICALDTADLERATTLAKAISGKVAMAKVGLEFFTAHGLSGVQKIVDLGFRIFLDLKLHDIPNTVRGALKSVVN
LPAIDMLTIHVSGGKNMIMGAIDALKECDIEPVGVTVLTSMDDVDLRACGINSSVEAHVMNLVDLAVSSGLNAIVCSAKE
VASIRKKYPSLKLVVPGIRMAQSATGDQKRVDTPKDTLAAGADYLVIGRVITQSSDPAGEIDKILKTL

Sequences:

>Translated_228_residues
MHQKNPIICALDTADLERATTLAKAISGKVAMAKVGLEFFTAHGLSGVQKIVDLGFRIFLDLKLHDIPNTVRGALKSVVN
LPAIDMLTIHVSGGKNMIMGAIDALKECDIEPVGVTVLTSMDDVDLRACGINSSVEAHVMNLVDLAVSSGLNAIVCSAKE
VASIRKKYPSLKLVVPGIRMAQSATGDQKRVDTPKDTLAAGADYLVIGRVITQSSDPAGEIDKILKTL
>Mature_228_residues
MHQKNPIICALDTADLERATTLAKAISGKVAMAKVGLEFFTAHGLSGVQKIVDLGFRIFLDLKLHDIPNTVRGALKSVVN
LPAIDMLTIHVSGGKNMIMGAIDALKECDIEPVGVTVLTSMDDVDLRACGINSSVEAHVMNLVDLAVSSGLNAIVCSAKE
VASIRKKYPSLKLVVPGIRMAQSATGDQKRVDTPKDTLAAGADYLVIGRVITQSSDPAGEIDKILKTL

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=230, Percent_Identity=39.5652173913043, Blast_Score=148, Evalue=2e-37,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 24144; Mature: 24144

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00589 PTS_HPR_SER ; PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHQKNPIICALDTADLERATTLAKAISGKVAMAKVGLEFFTAHGLSGVQKIVDLGFRIFL
CCCCCCEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEE
DLKLHDIPNTVRGALKSVVNLPAIDMLTIHVSGGKNMIMGAIDALKECDIEPVGVTVLTS
EEEECCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEC
MDDVDLRACGINSSVEAHVMNLVDLAVSSGLNAIVCSAKEVASIRKKYPSLKLVVPGIRM
CCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHCCCEEEEECCHHH
AQSATGDQKRVDTPKDTLAAGADYLVIGRVITQSSDPAGEIDKILKTL
HHCCCCCCCCCCCCHHHHHCCCCEEEEHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MHQKNPIICALDTADLERATTLAKAISGKVAMAKVGLEFFTAHGLSGVQKIVDLGFRIFL
CCCCCCEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEE
DLKLHDIPNTVRGALKSVVNLPAIDMLTIHVSGGKNMIMGAIDALKECDIEPVGVTVLTS
EEEECCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEC
MDDVDLRACGINSSVEAHVMNLVDLAVSSGLNAIVCSAKEVASIRKKYPSLKLVVPGIRM
CCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHCCCEEEEECCHHH
AQSATGDQKRVDTPKDTLAAGADYLVIGRVITQSSDPAGEIDKILKTL
HHCCCCCCCCCCCCHHHHHCCCCEEEEHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA