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The map label for this gene is fmt

Identifier: 88607450

GI number: 88607450

Start: 1026349

End: 1027254

Strand: Reverse

Name: fmt

Synonym: APH_0965

Alternate gene names: 88607450

Gene position: 1027254-1026349 (Counterclockwise)

Preceding gene: 88606917

Following gene: 88606732

Centisome position: 69.82

GC content: 42.49

Gene sequence:

>906_bases
ATGAAGGTAGTGTTTATGGGGTCTTCGGAGTTCTCACTTCCAGCTTTTGAGGCTTTAGTCTCTGCTGATTCCTATGATGT
TGTTGCCGTATATACAAAAGCGCCTAAGCCTGCGGGGCGTGGGTACGCGTTAACTAAGACTCCTGTTCATATTTGTGCTG
AGGGGAAGGGTATTCCCGTAAGAAGTCCTGTATCACTTAGGGCTGAAGGAGAAGAAAGCATAATGGCTGAGTATGCACCC
GACGTTATAGTGGTGGTTTCGTACGGGCTTATGTTACCCAAGTGGACATTGACTGCATCTCGAATGGGGTGTGTAAATAT
ACATCCTTCATTGTTACCCAGATGGCGTGGAGCTGCGCCTATGCAGCATGCTATTTTATCAGGGGATACGGTCACTGGGG
TGACTATTATGCAGATTAATGAATTTATGGATGCGGGAGATATATATCTTCAGGAAGTTACAGAAATAGGTGAAAAGGAG
AATATATTGGATCTTAGTAGAAGGCTTTCTGTAATGGGTAGTAGGATGCTCCTGAAAGTCTTGGATAGTATAGGGAGTAT
TCAACCTATTAAGCAGGACGAATCAGGGGTTACAATTGCTAATAAGTTAGAAGAATTTCGAATAGATTTTGAGGAAGCGG
CAGATGTGATATGCCGCAGGATTCGGGCTTTATATCCTAAAATGTATTTTATGCTTGATGGTTCTAGGGTTAGGGTTTTG
GAAGCTGAGAGCTATGAATTTGCAGAAATGAATATAGGGGATGTAATAAATAACGAGCTACACATAAGGTGTGGGGGGAA
TACAGCATTGGTTCCTCTGGTTGTACAGCAAGAGTCAAAAAAGCCATGTGATATTAAGAGCTTTTTGTGCAGGTTTAGAG
GCAAGTCTATGCCTGTTGTATCATAA

Upstream 100 bases:

>100_bases
TGGCGTGTTTGTCATGTCGACAATTTCTTAGTATGTGGGGGGAGATATCCTTTTAGTGGTTTGGCAGGGTAAGTGATAGA
ACGTTTTATTGGGGGGTGCC

Downstream 100 bases:

>100_bases
GTTGGCGATTATCTTTCAATTCGATAATTTTTCTTGAATCTGTTGTAGGGAGGGGGTATGTGACCTCTGGTCTCTCTGGT
CTCTCTGGTCTCTCTGGTCT

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MKVVFMGSSEFSLPAFEALVSADSYDVVAVYTKAPKPAGRGYALTKTPVHICAEGKGIPVRSPVSLRAEGEESIMAEYAP
DVIVVVSYGLMLPKWTLTASRMGCVNIHPSLLPRWRGAAPMQHAILSGDTVTGVTIMQINEFMDAGDIYLQEVTEIGEKE
NILDLSRRLSVMGSRMLLKVLDSIGSIQPIKQDESGVTIANKLEEFRIDFEEAADVICRRIRALYPKMYFMLDGSRVRVL
EAESYEFAEMNIGDVINNELHIRCGGNTALVPLVVQQESKKPCDIKSFLCRFRGKSMPVVS

Sequences:

>Translated_301_residues
MKVVFMGSSEFSLPAFEALVSADSYDVVAVYTKAPKPAGRGYALTKTPVHICAEGKGIPVRSPVSLRAEGEESIMAEYAP
DVIVVVSYGLMLPKWTLTASRMGCVNIHPSLLPRWRGAAPMQHAILSGDTVTGVTIMQINEFMDAGDIYLQEVTEIGEKE
NILDLSRRLSVMGSRMLLKVLDSIGSIQPIKQDESGVTIANKLEEFRIDFEEAADVICRRIRALYPKMYFMLDGSRVRVL
EAESYEFAEMNIGDVINNELHIRCGGNTALVPLVVQQESKKPCDIKSFLCRFRGKSMPVVS
>Mature_301_residues
MKVVFMGSSEFSLPAFEALVSADSYDVVAVYTKAPKPAGRGYALTKTPVHICAEGKGIPVRSPVSLRAEGEESIMAEYAP
DVIVVVSYGLMLPKWTLTASRMGCVNIHPSLLPRWRGAAPMQHAILSGDTVTGVTIMQINEFMDAGDIYLQEVTEIGEKE
NILDLSRRLSVMGSRMLLKVLDSIGSIQPIKQDESGVTIANKLEEFRIDFEEAADVICRRIRALYPKMYFMLDGSRVRVL
EAESYEFAEMNIGDVINNELHIRCGGNTALVPLVVQQESKKPCDIKSFLCRFRGKSMPVVS

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI164663775, Length=242, Percent_Identity=34.7107438016529, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI21614513, Length=233, Percent_Identity=27.8969957081545, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1789683, Length=292, Percent_Identity=38.013698630137, Blast_Score=191, Evalue=4e-50,
Organism=Escherichia coli, GI1788589, Length=274, Percent_Identity=27.007299270073, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI133930964, Length=266, Percent_Identity=24.812030075188, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319458, Length=175, Percent_Identity=32.5714285714286, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI45550868, Length=250, Percent_Identity=32.8, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28571984, Length=170, Percent_Identity=36.4705882352941, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_ANAPZ (Q2GJB8)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505533.1
- ProteinModelPortal:   Q2GJB8
- SMR:   Q2GJB8
- STRING:   Q2GJB8
- GeneID:   3930344
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0965
- NMPDR:   fig|212042.5.peg.912
- TIGR:   APH_0965
- eggNOG:   COG0223
- HOGENOM:   HBG571560
- OMA:   WRGAGPI
- ProtClustDB:   CLSK749560
- BioCyc:   APHA212042:APH_0965-MONOMER
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 33242; Mature: 33242

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVFMGSSEFSLPAFEALVSADSYDVVAVYTKAPKPAGRGYALTKTPVHICAEGKGIPV
CEEEEECCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEECCCEEEEECCCCCCC
RSPVSLRAEGEESIMAEYAPDVIVVVSYGLMLPKWTLTASRMGCVNIHPSLLPRWRGAAP
CCCCEEECCCCHHHHHHHCCCEEEEEEHHHHCCCEEEECCCCCEEECCHHHCCCCCCCCC
MQHAILSGDTVTGVTIMQINEFMDAGDIYLQEVTEIGEKENILDLSRRLSVMGSRMLLKV
HHHHHCCCCCCCCEEEEEEHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
LDSIGSIQPIKQDESGVTIANKLEEFRIDFEEAADVICRRIRALYPKMYFMLDGSRVRVL
HHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEEECCCEEEEE
EAESYEFAEMNIGDVINNELHIRCGGNTALVPLVVQQESKKPCDIKSFLCRFRGKSMPVV
ECCCCEEECCCHHHHCCCEEEEEECCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCCCCC
S
C
>Mature Secondary Structure
MKVVFMGSSEFSLPAFEALVSADSYDVVAVYTKAPKPAGRGYALTKTPVHICAEGKGIPV
CEEEEECCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEECCCEEEEECCCCCCC
RSPVSLRAEGEESIMAEYAPDVIVVVSYGLMLPKWTLTASRMGCVNIHPSLLPRWRGAAP
CCCCEEECCCCHHHHHHHCCCEEEEEEHHHHCCCEEEECCCCCEEECCHHHCCCCCCCCC
MQHAILSGDTVTGVTIMQINEFMDAGDIYLQEVTEIGEKENILDLSRRLSVMGSRMLLKV
HHHHHCCCCCCCCEEEEEEHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
LDSIGSIQPIKQDESGVTIANKLEEFRIDFEEAADVICRRIRALYPKMYFMLDGSRVRVL
HHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEEECCCEEEEE
EAESYEFAEMNIGDVINNELHIRCGGNTALVPLVVQQESKKPCDIKSFLCRFRGKSMPVV
ECCCCEEECCCHHHHCCCEEEEEECCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCCCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA