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The map label for this gene is hemF [H]

Identifier: 88607423

GI number: 88607423

Start: 436644

End: 437468

Strand: Direct

Name: hemF [H]

Synonym: APH_0423

Alternate gene names: 88607423

Gene position: 436644-437468 (Clockwise)

Preceding gene: 88607288

Following gene: 88607314

Centisome position: 29.68

GC content: 42.42

Gene sequence:

>825_bases
ATGGACGAGAAAAAAGATCTAGTTACTGAGTGGTTTGCTGTTTTAAGGGATAAAATAGTTAGTGCATTTCTTGCAGTAGA
GGCAGATTTTTCTGACAATGCTCCCACAATTAAGAAAAGAAAATGGGAAAGAGAAGGTGGCGGTGGCGGGGAGTCAGTTA
TCATTCTAGGATCTGTATTTGAAAAAGCGGGTGTTAATTTTTCTATAGTGCACGGAAAGCTGAAAAGCGACTTTGCTGCA
GAAATTCCAGGTGCACGAGAAAGTAATGGTGAATTTTGGGCGAGTGGCGTTTCTATTGTTGCACATATGTGTTCACCATT
TGTTCCAGCGATACACATGAATACAAGGTTTATTTGTACTTCAAAGAGTTGGTTTGGTGGTGGTATCGACCTTACTCCTG
TGTATCACAATGATGATGACACTATTTTCTTCCATGATGCCTTGAAAGCCATGTGTAATAAGTTTGATGCGCACTACTAC
GATCAGTTTAAAAAGAACTGTGACGAATATTTCTATCTTCCGCATAGGAAGGAGCCTAGGGGAGTCGGCGGAATATTCTA
CGATTATCTGCAGACTGGCTTTGAGAAGGACTTTGCCTTTACTCGCGCGGTTGGGGAATGTTTTCTAGAGGTTTATCCGG
CGATAGTGAGACGGAATATGCATAAGGAGTGGAGTGAAGAAGAGCGAGATTATCAGCTTATCAGTCGTGGTCGCTATGTA
GAGTTCAATTTGCTTTACGACCGTGGTACCAGGTTTGGATTAATGACGGACGGTAATTCCGATGCCATAATGATGTCTAT
GCCTCCGCTTGCGAAATGGGTTTAG

Upstream 100 bases:

>100_bases
TGCTACTATCTAGAGCATATTCTCTGTGGTAGTGGTAGAGGGGTGTTTTGTTTGTGCTCTTTAAATATGGATAGATTGTT
GATGTGTTGAGAGCTCTAAG

Downstream 100 bases:

>100_bases
GTTTGTATTGCTTTTTGTGATGTTCATGGCCATCTGGCAACGTGGTTTTGTGGCTTGTAGGATGGTTTTGTGCTGTCGCT
TTACGAACCGTAGTAGCAGG

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MDEKKDLVTEWFAVLRDKIVSAFLAVEADFSDNAPTIKKRKWEREGGGGGESVIILGSVFEKAGVNFSIVHGKLKSDFAA
EIPGARESNGEFWASGVSIVAHMCSPFVPAIHMNTRFICTSKSWFGGGIDLTPVYHNDDDTIFFHDALKAMCNKFDAHYY
DQFKKNCDEYFYLPHRKEPRGVGGIFYDYLQTGFEKDFAFTRAVGECFLEVYPAIVRRNMHKEWSEEERDYQLISRGRYV
EFNLLYDRGTRFGLMTDGNSDAIMMSMPPLAKWV

Sequences:

>Translated_274_residues
MDEKKDLVTEWFAVLRDKIVSAFLAVEADFSDNAPTIKKRKWEREGGGGGESVIILGSVFEKAGVNFSIVHGKLKSDFAA
EIPGARESNGEFWASGVSIVAHMCSPFVPAIHMNTRFICTSKSWFGGGIDLTPVYHNDDDTIFFHDALKAMCNKFDAHYY
DQFKKNCDEYFYLPHRKEPRGVGGIFYDYLQTGFEKDFAFTRAVGECFLEVYPAIVRRNMHKEWSEEERDYQLISRGRYV
EFNLLYDRGTRFGLMTDGNSDAIMMSMPPLAKWV
>Mature_274_residues
MDEKKDLVTEWFAVLRDKIVSAFLAVEADFSDNAPTIKKRKWEREGGGGGESVIILGSVFEKAGVNFSIVHGKLKSDFAA
EIPGARESNGEFWASGVSIVAHMCSPFVPAIHMNTRFICTSKSWFGGGIDLTPVYHNDDDTIFFHDALKAMCNKFDAHYY
DQFKKNCDEYFYLPHRKEPRGVGGIFYDYLQTGFEKDFAFTRAVGECFLEVYPAIVRRNMHKEWSEEERDYQLISRGRYV
EFNLLYDRGTRFGLMTDGNSDAIMMSMPPLAKWV

Specific function: Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III [H]

COG id: COG0408

COG function: function code H; Coproporphyrinogen III oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI41393599, Length=248, Percent_Identity=41.9354838709677, Blast_Score=198, Evalue=5e-51,
Organism=Escherichia coli, GI1788777, Length=283, Percent_Identity=44.1696113074205, Blast_Score=225, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6320249, Length=259, Percent_Identity=42.4710424710425, Blast_Score=186, Evalue=5e-48,
Organism=Drosophila melanogaster, GI17864388, Length=280, Percent_Identity=41.4285714285714, Blast_Score=205, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001260
- InterPro:   IPR018375 [H]

Pfam domain/function: PF01218 Coprogen_oxidas [H]

EC number: =1.3.3.3 [H]

Molecular weight: Translated: 31310; Mature: 31310

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEKKDLVTEWFAVLRDKIVSAFLAVEADFSDNAPTIKKRKWEREGGGGGESVIILGSVF
CCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHCCCCCCCCCCEEEEEEEHH
EKAGVNFSIVHGKLKSDFAAEIPGARESNGEFWASGVSIVAHMCSPFVPAIHMNTRFICT
HHHCCCEEEEECCHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCEEEEEE
SKSWFGGGIDLTPVYHNDDDTIFFHDALKAMCNKFDAHYYDQFKKNCDEYFYLPHRKEPR
CCCCCCCCEEECEEEECCCCEEEEHHHHHHHHHHHCHHHHHHHHCCCCCEEECCCCCCCC
GVGGIFYDYLQTGFEKDFAFTRAVGECFLEVYPAIVRRNMHKEWSEEERDYQLISRGRYV
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
EFNLLYDRGTRFGLMTDGNSDAIMMSMPPLAKWV
EEEEEEECCCEEEEEECCCCCEEEEECCCHHHCC
>Mature Secondary Structure
MDEKKDLVTEWFAVLRDKIVSAFLAVEADFSDNAPTIKKRKWEREGGGGGESVIILGSVF
CCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHCCCCCCCCCCEEEEEEEHH
EKAGVNFSIVHGKLKSDFAAEIPGARESNGEFWASGVSIVAHMCSPFVPAIHMNTRFICT
HHHCCCEEEEECCHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCEEEEEE
SKSWFGGGIDLTPVYHNDDDTIFFHDALKAMCNKFDAHYYDQFKKNCDEYFYLPHRKEPR
CCCCCCCCEEECEEEECCCCEEEEHHHHHHHHHHHCHHHHHHHHCCCCCEEECCCCCCCC
GVGGIFYDYLQTGFEKDFAFTRAVGECFLEVYPAIVRRNMHKEWSEEERDYQLISRGRYV
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
EFNLLYDRGTRFGLMTDGNSDAIMMSMPPLAKWV
EEEEEEECCCEEEEEECCCCCEEEEECCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA