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The map label for this gene is lpdA-2 [H]

Identifier: 88607350

GI number: 88607350

Start: 406012

End: 407418

Strand: Direct

Name: lpdA-2 [H]

Synonym: APH_0393

Alternate gene names: 88607350

Gene position: 406012-407418 (Clockwise)

Preceding gene: 88607659

Following gene: 88607877

Centisome position: 27.6

GC content: 45.56

Gene sequence:

>1407_bases
ATGGGTAGCAGTAACTACGATGTTGCCGTAATAGGTGCAGGTCCTGGGGGGTATAAATGCGCTATAAAGGCTGCCAAATT
GGGATTGAGTGTTGTTTGTATAGATAAGAATTCGCAATGGGGCGGTACCTGTCTTAGGGTTGGGTGTATTCCTTCAAAGG
CTATGTTGGAGTATTCTTACAAGTTTCACTCCGCGAAAGATCTCTTTCCTAAACTGGGAGTTATGGCCAAGGATGTGGCG
TTTGATCTCAAAAAGATGTTTGAGGTTAGGGATAATGAAATAGCTGTGCTGAGTTCGGGCATAGATGGCCTCTTTTCTGC
AGCTGGAGTTCATAAGTTACGTGCTGAAGCCAAGATAGCAGGAAAGAAAGGAGATTTCTTTGAAGTCGTGCTTTCGAATC
AAGATGGTTCTCTGGGGCAGGTATTAGCGAGAAACGTAGTGCTGGCTACGGGGTCTACTCCTACTTCTTTGCCGGGGATA
GATGTTGATGGGGATTCAGTTATATTTTCGGACGGGGCGCTAAGCATGGATGTCCCGAAGAGGCTACTGGTGATAGGAGG
CGGCGCCATAGGGCTTGAGATGTCTTCCATATGGAGTAGGTTAGGCTCCGAAGTGACGGTAGTAGAGTATGCTGATAAAA
TAGCTTCTGGATTTGATGCAGACATAAGCAAGGCGTTACAGGGTTTTCTGGAGAAGCAGGGTATCAAATTTAATTTGGCT
CAGAAGGTAGTATCTGTTGCCAAGGGAAATACAGGGTTGTTGGTTAATTGTGAATCTGTAGTTAATGGTGCTATGGCTTC
CATGGAAGTGGATAAAGTTCTGGTGGCTGTAGGTAGAAGTCCTAGTATTACCGGGGTTGTTGCTATGGATGGGCTGTTGC
TCGATAACCGAGGTTTCGTATGTGTTAACAATAGGTATGAAACCAGTATAAAGGGGATTTACGCGATAGGAGATGTTATT
GGTGGTGCTATGCTTGCTCACAAGGCGGAAATAGAGGGGCATGCAGTTGCTGAATTAATTGCTGGTAACGTGACGCAAGT
TGACTATGGGGTGATTCCTGCGGTTATATATACGCATCCTGCTGCGGCTTCTGTAGGTAGGGGTGAAGAGAGTCTTAAGA
GCGTAAACTACAAATATAAGGTTGGTAAGAGCAGCTTTGCTGCCAATGGTAGGGCTAGGGTTGCATGCGATAGTGATGGT
TTTGTCAAGGTGATAGCGTGCAAGGAGACTGATGTGATTTTGGGTGTGCATATAGTAGGTGCGCATGCTGACACTATGAT
TAATGAAGCTGCTGTTGCGTTGGGTTATAGAGCGACTGCTAAAGATATCTGCCATATTTGTCATTCGCATCCGGATGTGA
ATGAAGTATTTAGAGATGCATGCGAGGTTGTGTCTCATAAGCGCTAG

Upstream 100 bases:

>100_bases
ATCTCTGGGGCGCAAAGCCTGCGTTATCCTATTAAGGCTGATGCTTCGATATATGTCGCTGCTGGTAAGATGGGATTTGT
GATTGTTTCATAGGTGTTTT

Downstream 100 bases:

>100_bases
AGGTTGAGGTGGGTCCATTTACACTGCGTAGGAGATATGCAGTTGCGGCACTGTTAGTTGTGGGCTGTGTGTTATGGTTT
ACTAGCGGTATATTAATTAG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase 3; LPD-3 [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MGSSNYDVAVIGAGPGGYKCAIKAAKLGLSVVCIDKNSQWGGTCLRVGCIPSKAMLEYSYKFHSAKDLFPKLGVMAKDVA
FDLKKMFEVRDNEIAVLSSGIDGLFSAAGVHKLRAEAKIAGKKGDFFEVVLSNQDGSLGQVLARNVVLATGSTPTSLPGI
DVDGDSVIFSDGALSMDVPKRLLVIGGGAIGLEMSSIWSRLGSEVTVVEYADKIASGFDADISKALQGFLEKQGIKFNLA
QKVVSVAKGNTGLLVNCESVVNGAMASMEVDKVLVAVGRSPSITGVVAMDGLLLDNRGFVCVNNRYETSIKGIYAIGDVI
GGAMLAHKAEIEGHAVAELIAGNVTQVDYGVIPAVIYTHPAAASVGRGEESLKSVNYKYKVGKSSFAANGRARVACDSDG
FVKVIACKETDVILGVHIVGAHADTMINEAAVALGYRATAKDICHICHSHPDVNEVFRDACEVVSHKR

Sequences:

>Translated_468_residues
MGSSNYDVAVIGAGPGGYKCAIKAAKLGLSVVCIDKNSQWGGTCLRVGCIPSKAMLEYSYKFHSAKDLFPKLGVMAKDVA
FDLKKMFEVRDNEIAVLSSGIDGLFSAAGVHKLRAEAKIAGKKGDFFEVVLSNQDGSLGQVLARNVVLATGSTPTSLPGI
DVDGDSVIFSDGALSMDVPKRLLVIGGGAIGLEMSSIWSRLGSEVTVVEYADKIASGFDADISKALQGFLEKQGIKFNLA
QKVVSVAKGNTGLLVNCESVVNGAMASMEVDKVLVAVGRSPSITGVVAMDGLLLDNRGFVCVNNRYETSIKGIYAIGDVI
GGAMLAHKAEIEGHAVAELIAGNVTQVDYGVIPAVIYTHPAAASVGRGEESLKSVNYKYKVGKSSFAANGRARVACDSDG
FVKVIACKETDVILGVHIVGAHADTMINEAAVALGYRATAKDICHICHSHPDVNEVFRDACEVVSHKR
>Mature_467_residues
GSSNYDVAVIGAGPGGYKCAIKAAKLGLSVVCIDKNSQWGGTCLRVGCIPSKAMLEYSYKFHSAKDLFPKLGVMAKDVAF
DLKKMFEVRDNEIAVLSSGIDGLFSAAGVHKLRAEAKIAGKKGDFFEVVLSNQDGSLGQVLARNVVLATGSTPTSLPGID
VDGDSVIFSDGALSMDVPKRLLVIGGGAIGLEMSSIWSRLGSEVTVVEYADKIASGFDADISKALQGFLEKQGIKFNLAQ
KVVSVAKGNTGLLVNCESVVNGAMASMEVDKVLVAVGRSPSITGVVAMDGLLLDNRGFVCVNNRYETSIKGIYAIGDVIG
GAMLAHKAEIEGHAVAELIAGNVTQVDYGVIPAVIYTHPAAASVGRGEESLKSVNYKYKVGKSSFAANGRARVACDSDGF
VKVIACKETDVILGVHIVGAHADTMINEAAVALGYRATAKDICHICHSHPDVNEVFRDACEVVSHKR

Specific function: LPD-3 may substitute for lipoamide dehydrogenase of the 2-oxoglutarate dehydrogenase and pyruvate multienzyme complexes when the latter is inactive or missing [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=461, Percent_Identity=45.9869848156182, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI50301238, Length=471, Percent_Identity=29.0870488322718, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI33519430, Length=482, Percent_Identity=27.1784232365145, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI33519428, Length=482, Percent_Identity=27.1784232365145, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI33519426, Length=482, Percent_Identity=27.1784232365145, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI148277065, Length=482, Percent_Identity=27.1784232365145, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI148277071, Length=482, Percent_Identity=27.1784232365145, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI291045266, Length=479, Percent_Identity=26.3048016701461, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI22035672, Length=489, Percent_Identity=26.1758691206544, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI291045268, Length=472, Percent_Identity=26.0593220338983, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI21389617, Length=256, Percent_Identity=25.78125, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI226437568, Length=256, Percent_Identity=25.78125, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI65787454, Length=256, Percent_Identity=25.78125, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1786307, Length=463, Percent_Identity=35.85313174946, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI87082354, Length=462, Percent_Identity=27.4891774891775, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1789915, Length=450, Percent_Identity=26.6666666666667, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI87081717, Length=470, Percent_Identity=26.3829787234043, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1789065, Length=216, Percent_Identity=29.1666666666667, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI32565766, Length=464, Percent_Identity=45.6896551724138, Blast_Score=398, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI71983429, Length=447, Percent_Identity=26.1744966442953, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI71983419, Length=447, Percent_Identity=26.1744966442953, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17557007, Length=479, Percent_Identity=26.0960334029228, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI71982272, Length=496, Percent_Identity=25, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17559934, Length=203, Percent_Identity=29.5566502463054, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321091, Length=469, Percent_Identity=41.1513859275053, Blast_Score=347, Evalue=3e-96,
Organism=Saccharomyces cerevisiae, GI6325240, Length=473, Percent_Identity=31.0782241014799, Blast_Score=207, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6325166, Length=473, Percent_Identity=26.0042283298097, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI21358499, Length=459, Percent_Identity=44.6623093681917, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24640553, Length=501, Percent_Identity=28.7425149700599, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24640549, Length=502, Percent_Identity=28.6852589641434, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24640551, Length=500, Percent_Identity=29, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17737741, Length=484, Percent_Identity=27.8925619834711, Blast_Score=109, Evalue=4e-24,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49079; Mature: 48948

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSSNYDVAVIGAGPGGYKCAIKAAKLGLSVVCIDKNSQWGGTCLRVGCIPSKAMLEYSY
CCCCCCCEEEEECCCCCEEEEEEEECCCEEEEEEECCCCCCCEEEEEECCCCHHHHHHHH
KFHSAKDLFPKLGVMAKDVAFDLKKMFEVRDNEIAVLSSGIDGLFSAAGVHKLRAEAKIA
HHCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCC
GKKGDFFEVVLSNQDGSLGQVLARNVVLATGSTPTSLPGIDVDGDSVIFSDGALSMDVPK
CCCCCEEEEEEECCCCCHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEEECCCEECCCCC
RLLVIGGGAIGLEMSSIWSRLGSEVTVVEYADKIASGFDADISKALQGFLEKQGIKFNLA
EEEEEECCHHEEEHHHHHHHHCCCEEEEHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEHH
QKVVSVAKGNTGLLVNCESVVNGAMASMEVDKVLVAVGRSPSITGVVAMDGLLLDNRGFV
HHHHHHHCCCCCEEEEEHHHHCCHHHHHHHHHHHEEECCCCCEEEEEEECCEEECCCCEE
CVNNRYETSIKGIYAIGDVIGGAMLAHKAEIEGHAVAELIAGNVTQVDYGVIPAVIYTHP
EECCCCCCCCCHHEEHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEECCCCCCCEEEECC
AAASVGRGEESLKSVNYKYKVGKSSFAANGRARVACDSDGFVKVIACKETDVILGVHIVG
CHHCCCCCHHHHHHCCEEEEECCCCCCCCCCEEEEECCCCCEEEEEECCCCEEEEEEEEC
AHADTMINEAAVALGYRATAKDICHICHSHPDVNEVFRDACEVVSHKR
CCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GSSNYDVAVIGAGPGGYKCAIKAAKLGLSVVCIDKNSQWGGTCLRVGCIPSKAMLEYSY
CCCCCCEEEEECCCCCEEEEEEEECCCEEEEEEECCCCCCCEEEEEECCCCHHHHHHHH
KFHSAKDLFPKLGVMAKDVAFDLKKMFEVRDNEIAVLSSGIDGLFSAAGVHKLRAEAKIA
HHCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCC
GKKGDFFEVVLSNQDGSLGQVLARNVVLATGSTPTSLPGIDVDGDSVIFSDGALSMDVPK
CCCCCEEEEEEECCCCCHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEEECCCEECCCCC
RLLVIGGGAIGLEMSSIWSRLGSEVTVVEYADKIASGFDADISKALQGFLEKQGIKFNLA
EEEEEECCHHEEEHHHHHHHHCCCEEEEHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEHH
QKVVSVAKGNTGLLVNCESVVNGAMASMEVDKVLVAVGRSPSITGVVAMDGLLLDNRGFV
HHHHHHHCCCCCEEEEEHHHHCCHHHHHHHHHHHEEECCCCCEEEEEEECCEEECCCCEE
CVNNRYETSIKGIYAIGDVIGGAMLAHKAEIEGHAVAELIAGNVTQVDYGVIPAVIYTHP
EECCCCCCCCCHHEEHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEECCCCCCCEEEECC
AAASVGRGEESLKSVNYKYKVGKSSFAANGRARVACDSDGFVKVIACKETDVILGVHIVG
CHHCCCCCHHHHHHCCEEEEECCCCCCCCCCEEEEECCCCCEEEEEECCCCEEEEEEEEC
AHADTMINEAAVALGYRATAKDICHICHSHPDVNEVFRDACEVVSHKR
CCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]