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The map label for this gene is dnaB [H]

Identifier: 88607307

GI number: 88607307

Start: 306058

End: 307515

Strand: Direct

Name: dnaB [H]

Synonym: APH_0323

Alternate gene names: 88607307

Gene position: 306058-307515 (Clockwise)

Preceding gene: 88607166

Following gene: 88606854

Centisome position: 20.8

GC content: 42.59

Gene sequence:

>1458_bases
TTGGGGGATTTAGTGCATAGCTCAGAAGAAAAGGGCTTGATGAAAAGTATTGCTCACTTGCCTAACAATGTAGAAGCAGA
GCAGATGCTGATAGGTGCTATGCTCCGTGATAATAGGATCTGTGATGCTCTTGAGGATATGATCAGCTCGGAAGTATTTT
ATGATCCGTTACATGGACGCATTTTTGATCAAGTAGTGAAGATAAATAAGAGGGGTCTTTCTGCAAATGAGATAAGTCTC
AAGATGTTTTTTGACAATGATGAGGCGCTACTTGAGTGTGGAGGAGGGGAATATCTCGCAAAGATTGCGGCTAAGGCTAG
TGTAGTTTTTGACATCAGGAGTGTAGCGAGAGTAGTTTTTGATGCGCATCTTAGGCGATGTTTGATTTCTCTTGGTAGCG
ATATTGTTGGTAAGGCATACGATTATGATTCCGATAACCCAGCGGAGCAGCAGATAGAAAATGCTGCGCAGAAGCTTTTT
TTATTGGGGAATAAGGGGAAGGCTGATAGGAATTATCACACATTTGCGACGCTGATAGGAGATGTTAGGAAAAAGATTGA
GTCTGCCAGACATAATAAAGATGCTTTACAGGGGATTACGGTAGGTCTAAAAGACTTGGATTTATTGCTGAATGGATTGC
AAAATTCAGATTTGATTATTTTGGCTGCGCGGCCTTCTATGGGAAAAACAGCTTTGGCTTTGAATATGGCTCTTAGTGCG
TGTAAGTCGCTAAAGGAGAAGGGGCAGAGTGTAGGTTTTTTCTCTTTGGAAATGTCTGCGGAGCATATAGCATCTAGGCT
TATATCCATTGAGTCAGAGATCAGCTCATACAAAGCTTTATCCGGCAAAATAAGCAGTGGAGACTTACAGCAAGTTTTAA
GGGCTAGTGAAAATATATGTGATCTACCGCTAGTTATAGATGATTCTTCTGCGTTATCGATTAGTGGGCTTAGGACAAGG
ATCAGGAGAATGCACCAGCTGCATAATGTGGGGGTGATTTTTATTGACTATTTGCAGCTTATTAAGGGAACCACCAAAAG
GAGTGGTGATAATAGGGTGCAAGAAGTTTCTGAGGTGACTCAGGGGCTAAAAGCTATAGCTAAAGAATTGAATGTTCCTT
TGGTGGCACTCTCTCAGCTATCAAGGCTTGTTGAACAGCGGGATGATAAGAAGCCGCAGCTGTCGGATCTTCGTGATTCG
GGAAGTATTGAGCAGGATGCTGACGTAGTGATGTTTTTATATCGCGAGGAGTATTATGAGCTTAGAAAGCAGCCTACTGA
AGGGACTCCTAAGCATGCAGAGTGGCAGATGAAGATGGATTCTATTAGTAATCTTGCGGACGTTTTTGTTTCCAAGCAGA
GAAATGGTCCTATTGGTAATGTGAGGCTATTCTTTGAACCGGAGAAGGGTAAATTCCGCGATTACACGGCGCGCTATGGT
ATGTACGATCAGAGTTAG

Upstream 100 bases:

>100_bases
GTCAGCATGAAGGGGATGTATCAGGGCAAACACAAGGGGAGGTCGCGGTGAGATGTGTGGTTTGTGCTTGATTATGGATT
TAGGATGGCAGTCGTAGTTT

Downstream 100 bases:

>100_bases
TCTCTCAGGTTATCCAAGCTGTGATGTAGGAGTAAGCACGGTATTATTTGGATATATAAGCCATATAGTTCTCGCATTGT
TTGGTGGGATATTGCTGCAG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MGDLVHSSEEKGLMKSIAHLPNNVEAEQMLIGAMLRDNRICDALEDMISSEVFYDPLHGRIFDQVVKINKRGLSANEISL
KMFFDNDEALLECGGGEYLAKIAAKASVVFDIRSVARVVFDAHLRRCLISLGSDIVGKAYDYDSDNPAEQQIENAAQKLF
LLGNKGKADRNYHTFATLIGDVRKKIESARHNKDALQGITVGLKDLDLLLNGLQNSDLIILAARPSMGKTALALNMALSA
CKSLKEKGQSVGFFSLEMSAEHIASRLISIESEISSYKALSGKISSGDLQQVLRASENICDLPLVIDDSSALSISGLRTR
IRRMHQLHNVGVIFIDYLQLIKGTTKRSGDNRVQEVSEVTQGLKAIAKELNVPLVALSQLSRLVEQRDDKKPQLSDLRDS
GSIEQDADVVMFLYREEYYELRKQPTEGTPKHAEWQMKMDSISNLADVFVSKQRNGPIGNVRLFFEPEKGKFRDYTARYG
MYDQS

Sequences:

>Translated_485_residues
MGDLVHSSEEKGLMKSIAHLPNNVEAEQMLIGAMLRDNRICDALEDMISSEVFYDPLHGRIFDQVVKINKRGLSANEISL
KMFFDNDEALLECGGGEYLAKIAAKASVVFDIRSVARVVFDAHLRRCLISLGSDIVGKAYDYDSDNPAEQQIENAAQKLF
LLGNKGKADRNYHTFATLIGDVRKKIESARHNKDALQGITVGLKDLDLLLNGLQNSDLIILAARPSMGKTALALNMALSA
CKSLKEKGQSVGFFSLEMSAEHIASRLISIESEISSYKALSGKISSGDLQQVLRASENICDLPLVIDDSSALSISGLRTR
IRRMHQLHNVGVIFIDYLQLIKGTTKRSGDNRVQEVSEVTQGLKAIAKELNVPLVALSQLSRLVEQRDDKKPQLSDLRDS
GSIEQDADVVMFLYREEYYELRKQPTEGTPKHAEWQMKMDSISNLADVFVSKQRNGPIGNVRLFFEPEKGKFRDYTARYG
MYDQS
>Mature_484_residues
GDLVHSSEEKGLMKSIAHLPNNVEAEQMLIGAMLRDNRICDALEDMISSEVFYDPLHGRIFDQVVKINKRGLSANEISLK
MFFDNDEALLECGGGEYLAKIAAKASVVFDIRSVARVVFDAHLRRCLISLGSDIVGKAYDYDSDNPAEQQIENAAQKLFL
LGNKGKADRNYHTFATLIGDVRKKIESARHNKDALQGITVGLKDLDLLLNGLQNSDLIILAARPSMGKTALALNMALSAC
KSLKEKGQSVGFFSLEMSAEHIASRLISIESEISSYKALSGKISSGDLQQVLRASENICDLPLVIDDSSALSISGLRTRI
RRMHQLHNVGVIFIDYLQLIKGTTKRSGDNRVQEVSEVTQGLKAIAKELNVPLVALSQLSRLVEQRDDKKPQLSDLRDSG
SIEQDADVVMFLYREEYYELRKQPTEGTPKHAEWQMKMDSISNLADVFVSKQRNGPIGNVRLFFEPEKGKFRDYTARYGM
YDQS

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=466, Percent_Identity=35.1931330472103, Blast_Score=256, Evalue=3e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 54009; Mature: 53878

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDLVHSSEEKGLMKSIAHLPNNVEAEQMLIGAMLRDNRICDALEDMISSEVFYDPLHGR
CCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHH
IFDQVVKINKRGLSANEISLKMFFDNDEALLECGGGEYLAKIAAKASVVFDIRSVARVVF
HHHHHHHHHHCCCCCCCEEEEEEECCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
DAHLRRCLISLGSDIVGKAYDYDSDNPAEQQIENAAQKLFLLGNKGKADRNYHTFATLIG
HHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHH
DVRKKIESARHNKDALQGITVGLKDLDLLLNGLQNSDLIILAARPSMGKTALALNMALSA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHH
CKSLKEKGQSVGFFSLEMSAEHIASRLISIESEISSYKALSGKISSGDLQQVLRASENIC
HHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCC
DLPLVIDDSSALSISGLRTRIRRMHQLHNVGVIFIDYLQLIKGTTKRSGDNRVQEVSEVT
CCCEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
QGLKAIAKELNVPLVALSQLSRLVEQRDDKKPQLSDLRDSGSIEQDADVVMFLYREEYYE
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHH
LRKQPTEGTPKHAEWQMKMDSISNLADVFVSKQRNGPIGNVRLFFEPEKGKFRDYTARYG
HHCCCCCCCCCCCHHEEHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHCC
MYDQS
CCCCC
>Mature Secondary Structure 
GDLVHSSEEKGLMKSIAHLPNNVEAEQMLIGAMLRDNRICDALEDMISSEVFYDPLHGR
CCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHH
IFDQVVKINKRGLSANEISLKMFFDNDEALLECGGGEYLAKIAAKASVVFDIRSVARVVF
HHHHHHHHHHCCCCCCCEEEEEEECCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
DAHLRRCLISLGSDIVGKAYDYDSDNPAEQQIENAAQKLFLLGNKGKADRNYHTFATLIG
HHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHH
DVRKKIESARHNKDALQGITVGLKDLDLLLNGLQNSDLIILAARPSMGKTALALNMALSA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHH
CKSLKEKGQSVGFFSLEMSAEHIASRLISIESEISSYKALSGKISSGDLQQVLRASENIC
HHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCC
DLPLVIDDSSALSISGLRTRIRRMHQLHNVGVIFIDYLQLIKGTTKRSGDNRVQEVSEVT
CCCEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
QGLKAIAKELNVPLVALSQLSRLVEQRDDKKPQLSDLRDSGSIEQDADVVMFLYREEYYE
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHH
LRKQPTEGTPKHAEWQMKMDSISNLADVFVSKQRNGPIGNVRLFFEPEKGKFRDYTARYG
HHCCCCCCCCCCCHHEEHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHCC
MYDQS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA