Click here to switch to the map view.

The map label for this gene is folD

Identifier: 88607272

GI number: 88607272

Start: 175932

End: 176846

Strand: Direct

Name: folD

Synonym: APH_0175

Alternate gene names: 88607272

Gene position: 175932-176846 (Clockwise)

Preceding gene: 88607582

Following gene: 88607837

Centisome position: 11.96

GC content: 41.75

Gene sequence:

>915_bases
ATGGGTATATACGAAAACAATATAATAGATGGTAAGGCTGCTGCTGCGGATCTATTGGGAAGACTGAAAGTAGTAATGGA
GGGCTTGAAAGAGGAACATGGTTTATTTCCGTCGTTGGTAGTGATCATTGTTGGAGATGATCCCGCCAGCAGGTTGTACG
TGGGTAATAAACAAAGGAAGGCCGAAGAGCTTGGTATAAACTCCAAAACCATTGCGTTGCCATATGAGACTACTCAAGAA
GAATTATTGGGAATAATACAGGAACTGAATGAAGACGAAAATGTGCACGGTATTTTGGTACAGCTACCACTGCCTAAGCA
TATTGACAAAATCTTGATAATTAATGCCATTGATCCGGAAAAGGATGTTGATGGATTTCACAATGCAAATGCCGGCAAGT
TGCTTACAGGAGAGATGGATTGCATGGTGCCATGTACTCCGCAGGGATGTATATATCTGATCAAGAAAGTGATGCCTGTG
TTATCATCGAAGAGGGCTGTGGTTATCGGGAGATCAAATATCGTAGGAAAGCCCGTAGCGTTGTTGTTGATGTATGAAAA
TTGTACAGTTTCTATTTTACACTCTGCAACTGCTAATATAGAGGAGCATTGTCGTGATGCTGAGATAATAATTGCTGCAG
TGGGTAAAGCGCGGATGGTGAAGTCTGAATGGGTGAGTCCAGGAGCTGTAGTGATAGATGTCGGTATAAATTTGATCACA
AGTGAGAGCAAGAATAGATTCGTTGGTGATGTGGATTTTGAAAGTGTGCGAGATAAAGTGGCAGCTATTACTCCAGTGCC
AGGAGGAGTAGGGCCGATGACCATTGCATTTTTATTGGTCAATACCGTGCTTGGAGCATGTAGGCAGAAAGGCATAAAAC
AATATAAAAACATCAAAAGTTCAGTATTGCAGTAA

Upstream 100 bases:

>100_bases
GTTTGCTTTGTAACGTATGAGTTGAATGCAGGCGTTATTGGAATGTTGTGGTAGGTACCTTTGTTAGGGATATAGTGTTT
TTTGAAGAAAGTGGTGCGTT

Downstream 100 bases:

>100_bases
GCTAATTTATGGTTTAGAGTATGTACTGAAAAGGGCGGTTAGCTCAGTTGGTAGAGCGCCTCGTTTACACCGAGGAAGTC
AGCAGTTCGAGTCTGTTACT

Product: methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MGIYENNIIDGKAAAADLLGRLKVVMEGLKEEHGLFPSLVVIIVGDDPASRLYVGNKQRKAEELGINSKTIALPYETTQE
ELLGIIQELNEDENVHGILVQLPLPKHIDKILIINAIDPEKDVDGFHNANAGKLLTGEMDCMVPCTPQGCIYLIKKVMPV
LSSKRAVVIGRSNIVGKPVALLLMYENCTVSILHSATANIEEHCRDAEIIIAAVGKARMVKSEWVSPGAVVIDVGINLIT
SESKNRFVGDVDFESVRDKVAAITPVPGGVGPMTIAFLLVNTVLGACRQKGIKQYKNIKSSVLQ

Sequences:

>Translated_304_residues
MGIYENNIIDGKAAAADLLGRLKVVMEGLKEEHGLFPSLVVIIVGDDPASRLYVGNKQRKAEELGINSKTIALPYETTQE
ELLGIIQELNEDENVHGILVQLPLPKHIDKILIINAIDPEKDVDGFHNANAGKLLTGEMDCMVPCTPQGCIYLIKKVMPV
LSSKRAVVIGRSNIVGKPVALLLMYENCTVSILHSATANIEEHCRDAEIIIAAVGKARMVKSEWVSPGAVVIDVGINLIT
SESKNRFVGDVDFESVRDKVAAITPVPGGVGPMTIAFLLVNTVLGACRQKGIKQYKNIKSSVLQ
>Mature_303_residues
GIYENNIIDGKAAAADLLGRLKVVMEGLKEEHGLFPSLVVIIVGDDPASRLYVGNKQRKAEELGINSKTIALPYETTQEE
LLGIIQELNEDENVHGILVQLPLPKHIDKILIINAIDPEKDVDGFHNANAGKLLTGEMDCMVPCTPQGCIYLIKKVMPVL
SSKRAVVIGRSNIVGKPVALLLMYENCTVSILHSATANIEEHCRDAEIIIAAVGKARMVKSEWVSPGAVVIDVGINLITS
ESKNRFVGDVDFESVRDKVAAITPVPGGVGPMTIAFLLVNTVLGACRQKGIKQYKNIKSSVLQ

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=305, Percent_Identity=44.9180327868852, Blast_Score=254, Evalue=7e-68,
Organism=Homo sapiens, GI222418558, Length=295, Percent_Identity=43.728813559322, Blast_Score=212, Evalue=3e-55,
Organism=Homo sapiens, GI94721354, Length=312, Percent_Identity=42.6282051282051, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI36796743, Length=222, Percent_Identity=28.8288288288288, Blast_Score=93, Evalue=2e-19,
Organism=Escherichia coli, GI1786741, Length=280, Percent_Identity=42.5, Blast_Score=223, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI17568735, Length=268, Percent_Identity=44.0298507462687, Blast_Score=221, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6319558, Length=290, Percent_Identity=45.8620689655172, Blast_Score=251, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6321643, Length=296, Percent_Identity=42.9054054054054, Blast_Score=220, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6322933, Length=302, Percent_Identity=25.8278145695364, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17136816, Length=307, Percent_Identity=46.9055374592834, Blast_Score=240, Evalue=7e-64,
Organism=Drosophila melanogaster, GI17136818, Length=307, Percent_Identity=46.9055374592834, Blast_Score=240, Evalue=7e-64,
Organism=Drosophila melanogaster, GI24645718, Length=287, Percent_Identity=44.5993031358885, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI17137370, Length=287, Percent_Identity=44.5993031358885, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI62472483, Length=288, Percent_Identity=44.4444444444444, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI45551871, Length=288, Percent_Identity=44.4444444444444, Blast_Score=236, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_ANAPZ (Q2GLF2)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_504799.1
- ProteinModelPortal:   Q2GLF2
- SMR:   Q2GLF2
- STRING:   Q2GLF2
- GeneID:   3930546
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0175
- NMPDR:   fig|212042.5.peg.170
- TIGR:   APH_0175
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   CTPLGCI
- PhylomeDB:   Q2GLF2
- ProtClustDB:   CLSK747282
- BioCyc:   APHA212042:APH_0175-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 32882; Mature: 32751

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIYENNIIDGKAAAADLLGRLKVVMEGLKEEHGLFPSLVVIIVGDDPASRLYVGNKQRK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEECCCHHH
AEELGINSKTIALPYETTQEELLGIIQELNEDENVHGILVQLPLPKHIDKILIINAIDPE
HHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCHHCCEEEEEECCCCC
KDVDGFHNANAGKLLTGEMDCMVPCTPQGCIYLIKKVMPVLSSKRAVVIGRSNIVGKPVA
CCCCCCCCCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEE
LLLMYENCTVSILHSATANIEEHCRDAEIIIAAVGKARMVKSEWVSPGAVVIDVGINLIT
EEEEECCCEEEEEHHHHCCHHHHCCCCEEEEEECCCHHHHHHHCCCCCEEEEEECCEEEE
SESKNRFVGDVDFESVRDKVAAITPVPGGVGPMTIAFLLVNTVLGACRQKGIKQYKNIKS
CCCCCCEECCCCHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVLQ
HHCC
>Mature Secondary Structure 
GIYENNIIDGKAAAADLLGRLKVVMEGLKEEHGLFPSLVVIIVGDDPASRLYVGNKQRK
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEECCCHHH
AEELGINSKTIALPYETTQEELLGIIQELNEDENVHGILVQLPLPKHIDKILIINAIDPE
HHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCHHCCEEEEEECCCCC
KDVDGFHNANAGKLLTGEMDCMVPCTPQGCIYLIKKVMPVLSSKRAVVIGRSNIVGKPVA
CCCCCCCCCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEE
LLLMYENCTVSILHSATANIEEHCRDAEIIIAAVGKARMVKSEWVSPGAVVIDVGINLIT
EEEEECCCEEEEEHHHHCCHHHHCCCCEEEEEECCCHHHHHHHCCCCCEEEEEECCEEEE
SESKNRFVGDVDFESVRDKVAAITPVPGGVGPMTIAFLLVNTVLGACRQKGIKQYKNIKS
CCCCCCEECCCCHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVLQ
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA