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The map label for this gene is sppA [H]

Identifier: 88607225

GI number: 88607225

Start: 269025

End: 269921

Strand: Reverse

Name: sppA [H]

Synonym: APH_0272

Alternate gene names: 88607225

Gene position: 269921-269025 (Counterclockwise)

Preceding gene: 88607931

Following gene: 88607135

Centisome position: 18.35

GC content: 45.48

Gene sequence:

>897_bases
GTGTTCGATCACTTATTGAAGATTGAGGCTTTAGCTTCCAGGGTGTCAATGTGGCGTGCGCTTTTTTTTGTGGTCCTTGG
TTTATTGGTAGTTTTTGCCTCACAAGTGGATTATTCAAGATTCTCTTTTGCTGATATTGCTGGAGGTGCTGGCGGTTACG
TAGCGCGGATAGGGATAGAGGGTACGATAGGTCGGAATAAGGATCGCACTGCCCTACTAAGTAGGGTTGAGGATGACAGC
TCGATAAAGGCCGTAGTACTGAGGATAGATAGTCCTGGGGGTACTGTGGGAGATAGTGAGGAACTGTATAGGCAGATCCG
AGCTATAGCTGAAAAGAAGCCTGTAGTGGCGGTTATGGGAAATGTTGCAGCATCAGGTGGGTACATGACAGCCCTGGCTG
CAGATCATGTTATAGCCAATAATGGCACTATTACGGGTTCTATAGGGGTGCTAACACAATATGTAGGTGTTGCACGAATT
GCTGAGAGGCTAGGAATCACTTTGAAGACGATAAAGACTTCAGAACTGAAAGCGTCTATGTCACCTCTGGAGGAGATGAG
TAAGAATTCTGAGGATGTGATGCACGAACTTATAAAGGATTTTCATCACTTTTTTGTTAGCATGGTAGCGGAGAGAAGAG
GGTTATCTGAAGAAGAGGCTTATAAGGTTGCAGATGGTAGGGTTTATACTGGTGCTCAAGCGCTACAGGTGAAATTGGTA
GATGAGTTAGGTGGTGAGCGCGAAGCACTCGAGTGGTTGAAGTCTCATCATAATATTGACACGGAGAAGGTGGTAGTACG
CGACTTGGAGTATCAGAGTCGGTTAAGTTTGATGGGTGGTTTGCGTATGTTGCTTGAGTCATTCAAGCAGCCGTTATACG
CATTGCTTTTTGAATGA

Upstream 100 bases:

>100_bases
ATACCTGAAGGTAAGAAGTTTTTCCCTGGTGATAGGGTAGAAGCAGAAGTTTTAATGATAGGGTCATATAGTCTTGTTTT
ATCTCTAGAATAGGTTTCGT

Downstream 100 bases:

>100_bases
GAGCTGTATGACTTTGTTGAGATCTGATTTAATCAGGCGCATTGCAAGAAAGAATCCCACTCTTGGAGAGAGTGTGGTAT
CACAGATATTGAATGTGTTT

Product: signal peptide peptidase SppA, 36K type

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MFDHLLKIEALASRVSMWRALFFVVLGLLVVFASQVDYSRFSFADIAGGAGGYVARIGIEGTIGRNKDRTALLSRVEDDS
SIKAVVLRIDSPGGTVGDSEELYRQIRAIAEKKPVVAVMGNVAASGGYMTALAADHVIANNGTITGSIGVLTQYVGVARI
AERLGITLKTIKTSELKASMSPLEEMSKNSEDVMHELIKDFHHFFVSMVAERRGLSEEEAYKVADGRVYTGAQALQVKLV
DELGGEREALEWLKSHHNIDTEKVVVRDLEYQSRLSLMGGLRMLLESFKQPLYALLFE

Sequences:

>Translated_298_residues
MFDHLLKIEALASRVSMWRALFFVVLGLLVVFASQVDYSRFSFADIAGGAGGYVARIGIEGTIGRNKDRTALLSRVEDDS
SIKAVVLRIDSPGGTVGDSEELYRQIRAIAEKKPVVAVMGNVAASGGYMTALAADHVIANNGTITGSIGVLTQYVGVARI
AERLGITLKTIKTSELKASMSPLEEMSKNSEDVMHELIKDFHHFFVSMVAERRGLSEEEAYKVADGRVYTGAQALQVKLV
DELGGEREALEWLKSHHNIDTEKVVVRDLEYQSRLSLMGGLRMLLESFKQPLYALLFE
>Mature_298_residues
MFDHLLKIEALASRVSMWRALFFVVLGLLVVFASQVDYSRFSFADIAGGAGGYVARIGIEGTIGRNKDRTALLSRVEDDS
SIKAVVLRIDSPGGTVGDSEELYRQIRAIAEKKPVVAVMGNVAASGGYMTALAADHVIANNGTITGSIGVLTQYVGVARI
AERLGITLKTIKTSELKASMSPLEEMSKNSEDVMHELIKDFHHFFVSMVAERRGLSEEEAYKVADGRVYTGAQALQVKLV
DELGGEREALEWLKSHHNIDTEKVVVRDLEYQSRLSLMGGLRMLLESFKQPLYALLFE

Specific function: Digestion of cleaved signal peptides. Required for efficient processing of precursors under conditions of hyper-secretion [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=173, Percent_Identity=35.8381502890173, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1787527, Length=167, Percent_Identity=31.1377245508982, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 32729; Mature: 32729

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDHLLKIEALASRVSMWRALFFVVLGLLVVFASQVDYSRFSFADIAGGAGGYVARIGIE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEECC
GTIGRNKDRTALLSRVEDDSSIKAVVLRIDSPGGTVGDSEELYRQIRAIAEKKPVVAVMG
CCCCCCCHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEC
NVAASGGYMTALAADHVIANNGTITGSIGVLTQYVGVARIAERLGITLKTIKTSELKASM
CHHCCCCCHHHHHHHHEEECCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHH
SPLEEMSKNSEDVMHELIKDFHHFFVSMVAERRGLSEEEAYKVADGRVYTGAQALQVKLV
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCHHHHHEEEH
DELGGEREALEWLKSHHNIDTEKVVVRDLEYQSRLSLMGGLRMLLESFKQPLYALLFE
HHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFDHLLKIEALASRVSMWRALFFVVLGLLVVFASQVDYSRFSFADIAGGAGGYVARIGIE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEECC
GTIGRNKDRTALLSRVEDDSSIKAVVLRIDSPGGTVGDSEELYRQIRAIAEKKPVVAVMG
CCCCCCCHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEC
NVAASGGYMTALAADHVIANNGTITGSIGVLTQYVGVARIAERLGITLKTIKTSELKASM
CHHCCCCCHHHHHHHHEEECCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHH
SPLEEMSKNSEDVMHELIKDFHHFFVSMVAERRGLSEEEAYKVADGRVYTGAQALQVKLV
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCHHHHHEEEH
DELGGEREALEWLKSHHNIDTEKVVVRDLEYQSRLSLMGGLRMLLESFKQPLYALLFE
HHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 10455123 [H]