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The map label for this gene is bioA [H]

Identifier: 88607198

GI number: 88607198

Start: 509908

End: 511179

Strand: Direct

Name: bioA [H]

Synonym: APH_0482

Alternate gene names: 88607198

Gene position: 509908-511179 (Clockwise)

Preceding gene: 88606794

Following gene: 88607939

Centisome position: 34.66

GC content: 41.75

Gene sequence:

>1272_bases
ATGTACGAACATGCACATTGGTTTCAAGAAGAGAAAGTATTATTCCCCTACAGTAGCGCAGTGCCTGCAGTGGCCCCGAT
GAGAGTGGTGTCAGGCCAGGGAAGTTATCTAGAACTTGAAGATGGCAAAAGGCTATTGGATGGTATATCGAGCTGGTGGA
GTGTATGTCATGGCTATTCGCATCCTTATATAGTAGGCAAAATGCAGGAGCAATTGGCAAAGTTATCACATGTGATGTTT
TGCCGTGATTTAATTCATGAAGGAGCATACGTACTAGCATCACGCATCGCTAAGTTAATGCCGCCAGGGCTTGATAGGGT
GTTTTTTGCGGATTCTGGATCTATGGCAGTGGAAGTAGCGCTGAAACTCGCTGTTCAATACTGGTACAGTATGGGTAAGC
GAGAAAAACACAGCTTTATATATTTCAAAAATTCATACCATGGGGATAGCATGGGATGTATATCGATCTCTGATCCTGCA
GCTATTCATGGAGACTCCTTTACAAGATATTGTCCGAAGCAATATCTGCTTGATATACCCGCAAGTGAAGAAGACGTAGT
ATTGTTGCAGCAGAAGATAGAGAGCATAGCGGATAAAGTTGCCGCAATTATCGTCGAGCCACTTTTACAGGCTGCAGGTG
GTATGGTGATTTATCCTCCTCATGTTTTATCTACGTTGAGAAAGATTGCGAAAGAAAATGAGATTCTGTTTATTGCTGAC
GAAGTTGCTACAGGCTTTTATAGATTAGGAACATCCTTTGCTTGTGAGCAGGCTTCTATTCAGCCTGATATTATGGTAAT
AGGAAAAGCCCTTAGCGGCGGTACTTGTCCACTATCAGCAGCGGTAGTTAGCAGTAATATATCCGAACTTTTTATATCCG
GTGGAGAAACATTTATGCACGGTAATACATTTATGGCACATCCTTTATCTTGTGCTGCTGCTAATGCTTCTCTCGATTTA
TTTGCGGGTGAGTCGTACACTCAGAAGGTATCGGGAATAGAAAGAATTTTAAAAGCCGAATTGGAAGAACTGCATGCTTT
AGATTACGTGTGCAATGTGAGAATAAAAGGGGCTATGGCAGCTTTTAATATTAAGAACGAGTGCGTGGAAAAGCTTAAAA
ATGGCATGACACAAAAGCTCTTAGATTTGGGTATATGGATCAGGCCAATTAAAACTGTAATGTATGTGATGCCACCGCTC
ACAATTGCAGAGGATGAGCTATTAGCATTGCTTTCTGCATTGAAAACACTGGTATACGAGTGTAGAAGATAA

Upstream 100 bases:

>100_bases
ATAGACTCTCAAATCGTCACTCTAGTGTACATATATAGAGTACGACTACTGTGGAAAATGTGTTACCATACTTCCTATTT
TATGGGAGTTGTGAGGTTCC

Downstream 100 bases:

>100_bases
GTACTTAGTATTTTAGGCAAAAATACTGGAAGGTTTATTTTCAGCCGTACTGTTGCTTCTGGATGTGTATGTTGCAAGAT
GATGGGTGTACACGAATTAA

Product: adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MYEHAHWFQEEKVLFPYSSAVPAVAPMRVVSGQGSYLELEDGKRLLDGISSWWSVCHGYSHPYIVGKMQEQLAKLSHVMF
CRDLIHEGAYVLASRIAKLMPPGLDRVFFADSGSMAVEVALKLAVQYWYSMGKREKHSFIYFKNSYHGDSMGCISISDPA
AIHGDSFTRYCPKQYLLDIPASEEDVVLLQQKIESIADKVAAIIVEPLLQAAGGMVIYPPHVLSTLRKIAKENEILFIAD
EVATGFYRLGTSFACEQASIQPDIMVIGKALSGGTCPLSAAVVSSNISELFISGGETFMHGNTFMAHPLSCAAANASLDL
FAGESYTQKVSGIERILKAELEELHALDYVCNVRIKGAMAAFNIKNECVEKLKNGMTQKLLDLGIWIRPIKTVMYVMPPL
TIAEDELLALLSALKTLVYECRR

Sequences:

>Translated_423_residues
MYEHAHWFQEEKVLFPYSSAVPAVAPMRVVSGQGSYLELEDGKRLLDGISSWWSVCHGYSHPYIVGKMQEQLAKLSHVMF
CRDLIHEGAYVLASRIAKLMPPGLDRVFFADSGSMAVEVALKLAVQYWYSMGKREKHSFIYFKNSYHGDSMGCISISDPA
AIHGDSFTRYCPKQYLLDIPASEEDVVLLQQKIESIADKVAAIIVEPLLQAAGGMVIYPPHVLSTLRKIAKENEILFIAD
EVATGFYRLGTSFACEQASIQPDIMVIGKALSGGTCPLSAAVVSSNISELFISGGETFMHGNTFMAHPLSCAAANASLDL
FAGESYTQKVSGIERILKAELEELHALDYVCNVRIKGAMAAFNIKNECVEKLKNGMTQKLLDLGIWIRPIKTVMYVMPPL
TIAEDELLALLSALKTLVYECRR
>Mature_423_residues
MYEHAHWFQEEKVLFPYSSAVPAVAPMRVVSGQGSYLELEDGKRLLDGISSWWSVCHGYSHPYIVGKMQEQLAKLSHVMF
CRDLIHEGAYVLASRIAKLMPPGLDRVFFADSGSMAVEVALKLAVQYWYSMGKREKHSFIYFKNSYHGDSMGCISISDPA
AIHGDSFTRYCPKQYLLDIPASEEDVVLLQQKIESIADKVAAIIVEPLLQAAGGMVIYPPHVLSTLRKIAKENEILFIAD
EVATGFYRLGTSFACEQASIQPDIMVIGKALSGGTCPLSAAVVSSNISELFISGGETFMHGNTFMAHPLSCAAANASLDL
FAGESYTQKVSGIERILKAELEELHALDYVCNVRIKGAMAAFNIKNECVEKLKNGMTQKLLDLGIWIRPIKTVMYVMPPL
TIAEDELLALLSALKTLVYECRR

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=389, Percent_Identity=32.133676092545, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI284507298, Length=304, Percent_Identity=33.5526315789474, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI13994255, Length=368, Percent_Identity=26.9021739130435, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI37574042, Length=332, Percent_Identity=27.4096385542169, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI226442705, Length=332, Percent_Identity=26.5060240963855, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI24119277, Length=328, Percent_Identity=25.9146341463415, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI226442709, Length=285, Percent_Identity=27.3684210526316, Blast_Score=93, Evalue=5e-19,
Organism=Escherichia coli, GI1786991, Length=423, Percent_Identity=40.1891252955083, Blast_Score=294, Evalue=6e-81,
Organism=Escherichia coli, GI145693181, Length=365, Percent_Identity=30.1369863013699, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1789759, Length=336, Percent_Identity=28.8690476190476, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1787560, Length=404, Percent_Identity=27.4752475247525, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789016, Length=404, Percent_Identity=26.980198019802, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788044, Length=398, Percent_Identity=26.8844221105528, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786349, Length=392, Percent_Identity=25, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25144271, Length=395, Percent_Identity=29.620253164557, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI25144274, Length=277, Percent_Identity=31.4079422382672, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71992977, Length=434, Percent_Identity=26.9585253456221, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324386, Length=465, Percent_Identity=31.8279569892473, Blast_Score=220, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6324432, Length=376, Percent_Identity=28.4574468085106, Blast_Score=133, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6323470, Length=405, Percent_Identity=28.3950617283951, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6321456, Length=364, Percent_Identity=25.2747252747253, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21357415, Length=389, Percent_Identity=30.5912596401028, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI21356575, Length=430, Percent_Identity=28.1395348837209, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI161085790, Length=433, Percent_Identity=27.0207852193995, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28574759, Length=369, Percent_Identity=27.3712737127371, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR005814
- InterPro:   IPR005815
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR007197 [H]

Pfam domain/function: PF00202 Aminotran_3; PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 46751; Mature: 46751

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00079 MULTICOPPER_OXIDASE1 ; PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEHAHWFQEEKVLFPYSSAVPAVAPMRVVSGQGSYLELEDGKRLLDGISSWWSVCHGYS
CCCCHHHHCCCCEECCCCCCCCCCCCHHEECCCCCEEEECCCHHHHHHHHHHHHHHCCCC
HPYIVGKMQEQLAKLSHVMFCRDLIHEGAYVLASRIAKLMPPGLDRVFFADSGSMAVEVA
CCEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHH
LKLAVQYWYSMGKREKHSFIYFKNSYHGDSMGCISISDPAAIHGDSFTRYCPKQYLLDIP
HHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEECCCCEECCCCHHHHCCHHHEEECC
ASEEDVVLLQQKIESIADKVAAIIVEPLLQAAGGMVIYPPHVLSTLRKIAKENEILFIAD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHCCCCEEEEEH
EVATGFYRLGTSFACEQASIQPDIMVIGKALSGGTCPLSAAVVSSNISELFISGGETFMH
HHHHHHHHHCCCHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCHHHHHHCCCCEEEE
GNTFMAHPLSCAAANASLDLFAGESYTQKVSGIERILKAELEELHALDYVCNVRIKGAMA
CCCEEECCCHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECHHE
AFNIKNECVEKLKNGMTQKLLDLGIWIRPIKTVMYVMPPLTIAEDELLALLSALKTLVYE
EECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
CRR
HCC
>Mature Secondary Structure
MYEHAHWFQEEKVLFPYSSAVPAVAPMRVVSGQGSYLELEDGKRLLDGISSWWSVCHGYS
CCCCHHHHCCCCEECCCCCCCCCCCCHHEECCCCCEEEECCCHHHHHHHHHHHHHHCCCC
HPYIVGKMQEQLAKLSHVMFCRDLIHEGAYVLASRIAKLMPPGLDRVFFADSGSMAVEVA
CCEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHH
LKLAVQYWYSMGKREKHSFIYFKNSYHGDSMGCISISDPAAIHGDSFTRYCPKQYLLDIP
HHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEECCCCEECCCCHHHHCCHHHEEECC
ASEEDVVLLQQKIESIADKVAAIIVEPLLQAAGGMVIYPPHVLSTLRKIAKENEILFIAD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHCCCCEEEEEH
EVATGFYRLGTSFACEQASIQPDIMVIGKALSGGTCPLSAAVVSSNISELFISGGETFMH
HHHHHHHHHCCCHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCHHHHHHCCCCEEEE
GNTFMAHPLSCAAANASLDLFAGESYTQKVSGIERILKAELEELHALDYVCNVRIKGAMA
CCCEEECCCHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECHHE
AFNIKNECVEKLKNGMTQKLLDLGIWIRPIKTVMYVMPPLTIAEDELLALLSALKTLVYE
EECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
CRR
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]