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The map label for this gene is leuS [H]

Identifier: 88607061

GI number: 88607061

Start: 549909

End: 552422

Strand: Reverse

Name: leuS [H]

Synonym: APH_0534

Alternate gene names: 88607061

Gene position: 552422-549909 (Counterclockwise)

Preceding gene: 88606925

Following gene: 88607842

Centisome position: 37.55

GC content: 43.6

Gene sequence:

>2514_bases
GTGGAATATACAAAACACCGGGGCCCATTGTACATGAGTTATGATTTTAAAAATATAGAGAGTGAGATACAACAGCGGTG
CTGCTTTACTATTGACAAAAGCGAAAATATAAACTGCTATGTCCTTAGTATGTTCCCATATCCTTCGGGAAATATACATA
TGGGACACATACGTAATTACGTCATAGGGGACGTAATAGCTCGGTTTAAGCGTGCAAAGGGGTTTTCTGTTCTGCACCCC
ATAGGATGGGACTCTTTTGGTCTTCCGGCTGAAAATGCCGCTCTGTCCTATGGTATTAACCCTGAATTGTGGACGAGGAA
TAATATAGATTCCATGCGAACACAGCTCAAAGCGATAGGTATCTCATATAACTGGGATCGGGAAATTACTACCTGCTCGG
AAGACTACTATAAGCATGAACAAAGTTTCTTCATCGACTTTTTACAGAATGGATTAGCCTACCGCAAGGAATCATGGGTT
AATTGGGATCCGGTAGATAATACAGTTTTAGCTAACGAGCAGGTAATTGATGGCAGAGGTTGGCGCTCCGGTGCGCTGGT
AGAGCGTCGCAAGTTATCCCAGTGGTTTTTGAGGATTACTGATTACGCTCAAAGCCTTCTGGATGGGTTGAATCATCTCC
CAGGGTGGCCTGAAAAGGTTAAGCTTATGCAGCAGCGCTGGATTGGGAGAAGCGAAGGCGTTGTAATAGAATTTGATACG
GACTGTGAGCAAAAGTTAGAAGTGTTCTCCACCATGCCTCATATGCTTTTTGGAGCGACGTTCTGCGCTGTTTCCATTGA
TCATCCTATATTGCAATATGCTAAAGCAGCAGGATTTGCTGAACGTATTAATGAGCTCACGAAACCTGCTCTGCAGTGCA
AAGATGCGGAAAAGGCGGGTATAGACACAGGACTGGTAGCAAAGCACCCGTTTTTAGACAAGAAATTGCCTATTTACGTC
GCGAATTATGTTCTTGAAGACTCTGGCACAGGTGCGGTGTTTGGATGCCCTGCTCATGATCAAAGGGATTTTGAATTTGC
CAAGCTTTATGGATTACCAATACAGCAAGTGGTGTTTCCTGAAGATGGTTCGGTATGTAACTTAGAGGAAAGAGCATACA
CTGGGGATGGGGTATACTGTAATTCCGGCTTTCTTGATGGAGTACGTCTTGGCGATGCTAAACGTGTAATGCTGGAAAAG
CTGGAGTCACTTGCCAATTGTAAGCAAGTAACCAACTATAGGCTGCATGACTGGGGAATATCACGGCAGAGATATTGGGG
GTGCCCTATTCCGATAATATATTGCACGCAGTGCGGAATAGTACCCGTGGATAAGAAGGATTTACCCGTGCGGTTACCAA
AAGATGTAGATTTCTCAAAAGGTGGCAATCCGCTCGCAAATCATCCAACATGGAAACACGTAAAATGCCACGTTTGTGGG
GGAGATGCAGAACGCGAAACTGATACTTTTGATACCTTTTTTGAGTCTTCATGGTACTTTGCAGCATTTTGCAGCAGACA
AAACGAGTTAAACCTGTCTGATAGCAACAGAATTCTGCCGGTGGATTACTATATAGGTGGCATAGAGCACGCTGTTCTAC
ATTTGCTTTACTCTCGATTTTTCTGCAGAGCGCTGGGTAAATGCGGACACTTACAAATCGAGGAGCCTTTCCGGAATCTT
ATTACGCAGGGTATGGTTTGTCACACCACATATCAAGACGGGTCTGGGAATTATCTTTTTCCCAAGGATGCCGAAAAAAT
GGCTGCTGAAGGTAAAAAAGTCCTTCAAGGCAAAGTTGAGAAAATGAGTAAATCTAAAAAAAATGTGGTTGATCCCAGCG
AGATACTCAAGCAGTACGGAGCAGATACTGTAAGATTGTTCATGTTATCAGATACTCCTCCAGAGCGGGATATCGAATGG
ACTGATTGTGGTGTCGAAGGTGCATGGCGGTATTTGGAGCGCTTGTGGAAGGTTTTAGGAAGTAATACTAGTATTAGCAC
TGAGTTTAACAATGAGAACGTAAGTGGCAAAGATCTTGAGTTCCTGTCTAAAGTACACAAACTTCTCAATGGCATTGAGG
GTGATATAGAGCACTGTAGGCTAAATTGTGCCGTAGCAAAATTCAGAGAGATGAGCAACATAGTTTTTGAAATGATGAAG
TGTTCAGCTAGCAATTCCGTCATTAATGAATCTGTGTGCATTTTACTGAGAGTTATGGAACCATTTATTCCTCATTTGAC
TGCGAAGTTATGGGAGATTATCGGCGGAAGAGGCATGCTTTGTGAGCAGCCTTGGCCCAGTGTAAGAAAAGCCCTACTCG
AAGAAAACTTTGCTACCATTGCCGTTCAAGTAGATGGCAAATTTTGCGGTACTCTTAGGGTAGAGTTGCAATGTGACGAT
GACAAGGTCAAGACCGAAGCCTTAGAACTTGCGCAACGCAGAATAAAAGAGCGTTTGGTAAAGAACGTGCACTATGTGCC
TGGAAAGGTAGTGAACATTGTTACAAAGGCCTAA

Upstream 100 bases:

>100_bases
AACAATACTACGCTTTGCTCATGTATTGCTCTTCTAGATTATATGGTATAGTTAGCAAATGGTGACGCGCACTCTGTATG
CAAAATCACCAAGCATTTTA

Downstream 100 bases:

>100_bases
GATTGATTATGTGTAACGATAATGCGCTATGGAGGTGCAATATGAGAAGGACTATGTTTTGTGGCAGCCTTGGCCCCGTG
TAAGAAAGGCCCTACTCGAA

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 837; Mature: 837

Protein sequence:

>837_residues
MEYTKHRGPLYMSYDFKNIESEIQQRCCFTIDKSENINCYVLSMFPYPSGNIHMGHIRNYVIGDVIARFKRAKGFSVLHP
IGWDSFGLPAENAALSYGINPELWTRNNIDSMRTQLKAIGISYNWDREITTCSEDYYKHEQSFFIDFLQNGLAYRKESWV
NWDPVDNTVLANEQVIDGRGWRSGALVERRKLSQWFLRITDYAQSLLDGLNHLPGWPEKVKLMQQRWIGRSEGVVIEFDT
DCEQKLEVFSTMPHMLFGATFCAVSIDHPILQYAKAAGFAERINELTKPALQCKDAEKAGIDTGLVAKHPFLDKKLPIYV
ANYVLEDSGTGAVFGCPAHDQRDFEFAKLYGLPIQQVVFPEDGSVCNLEERAYTGDGVYCNSGFLDGVRLGDAKRVMLEK
LESLANCKQVTNYRLHDWGISRQRYWGCPIPIIYCTQCGIVPVDKKDLPVRLPKDVDFSKGGNPLANHPTWKHVKCHVCG
GDAERETDTFDTFFESSWYFAAFCSRQNELNLSDSNRILPVDYYIGGIEHAVLHLLYSRFFCRALGKCGHLQIEEPFRNL
ITQGMVCHTTYQDGSGNYLFPKDAEKMAAEGKKVLQGKVEKMSKSKKNVVDPSEILKQYGADTVRLFMLSDTPPERDIEW
TDCGVEGAWRYLERLWKVLGSNTSISTEFNNENVSGKDLEFLSKVHKLLNGIEGDIEHCRLNCAVAKFREMSNIVFEMMK
CSASNSVINESVCILLRVMEPFIPHLTAKLWEIIGGRGMLCEQPWPSVRKALLEENFATIAVQVDGKFCGTLRVELQCDD
DKVKTEALELAQRRIKERLVKNVHYVPGKVVNIVTKA

Sequences:

>Translated_837_residues
MEYTKHRGPLYMSYDFKNIESEIQQRCCFTIDKSENINCYVLSMFPYPSGNIHMGHIRNYVIGDVIARFKRAKGFSVLHP
IGWDSFGLPAENAALSYGINPELWTRNNIDSMRTQLKAIGISYNWDREITTCSEDYYKHEQSFFIDFLQNGLAYRKESWV
NWDPVDNTVLANEQVIDGRGWRSGALVERRKLSQWFLRITDYAQSLLDGLNHLPGWPEKVKLMQQRWIGRSEGVVIEFDT
DCEQKLEVFSTMPHMLFGATFCAVSIDHPILQYAKAAGFAERINELTKPALQCKDAEKAGIDTGLVAKHPFLDKKLPIYV
ANYVLEDSGTGAVFGCPAHDQRDFEFAKLYGLPIQQVVFPEDGSVCNLEERAYTGDGVYCNSGFLDGVRLGDAKRVMLEK
LESLANCKQVTNYRLHDWGISRQRYWGCPIPIIYCTQCGIVPVDKKDLPVRLPKDVDFSKGGNPLANHPTWKHVKCHVCG
GDAERETDTFDTFFESSWYFAAFCSRQNELNLSDSNRILPVDYYIGGIEHAVLHLLYSRFFCRALGKCGHLQIEEPFRNL
ITQGMVCHTTYQDGSGNYLFPKDAEKMAAEGKKVLQGKVEKMSKSKKNVVDPSEILKQYGADTVRLFMLSDTPPERDIEW
TDCGVEGAWRYLERLWKVLGSNTSISTEFNNENVSGKDLEFLSKVHKLLNGIEGDIEHCRLNCAVAKFREMSNIVFEMMK
CSASNSVINESVCILLRVMEPFIPHLTAKLWEIIGGRGMLCEQPWPSVRKALLEENFATIAVQVDGKFCGTLRVELQCDD
DKVKTEALELAQRRIKERLVKNVHYVPGKVVNIVTKA
>Mature_837_residues
MEYTKHRGPLYMSYDFKNIESEIQQRCCFTIDKSENINCYVLSMFPYPSGNIHMGHIRNYVIGDVIARFKRAKGFSVLHP
IGWDSFGLPAENAALSYGINPELWTRNNIDSMRTQLKAIGISYNWDREITTCSEDYYKHEQSFFIDFLQNGLAYRKESWV
NWDPVDNTVLANEQVIDGRGWRSGALVERRKLSQWFLRITDYAQSLLDGLNHLPGWPEKVKLMQQRWIGRSEGVVIEFDT
DCEQKLEVFSTMPHMLFGATFCAVSIDHPILQYAKAAGFAERINELTKPALQCKDAEKAGIDTGLVAKHPFLDKKLPIYV
ANYVLEDSGTGAVFGCPAHDQRDFEFAKLYGLPIQQVVFPEDGSVCNLEERAYTGDGVYCNSGFLDGVRLGDAKRVMLEK
LESLANCKQVTNYRLHDWGISRQRYWGCPIPIIYCTQCGIVPVDKKDLPVRLPKDVDFSKGGNPLANHPTWKHVKCHVCG
GDAERETDTFDTFFESSWYFAAFCSRQNELNLSDSNRILPVDYYIGGIEHAVLHLLYSRFFCRALGKCGHLQIEEPFRNL
ITQGMVCHTTYQDGSGNYLFPKDAEKMAAEGKKVLQGKVEKMSKSKKNVVDPSEILKQYGADTVRLFMLSDTPPERDIEW
TDCGVEGAWRYLERLWKVLGSNTSISTEFNNENVSGKDLEFLSKVHKLLNGIEGDIEHCRLNCAVAKFREMSNIVFEMMK
CSASNSVINESVCILLRVMEPFIPHLTAKLWEIIGGRGMLCEQPWPSVRKALLEENFATIAVQVDGKFCGTLRVELQCDD
DKVKTEALELAQRRIKERLVKNVHYVPGKVVNIVTKA

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=854, Percent_Identity=36.6510538641686, Blast_Score=506, Evalue=1e-143,
Organism=Escherichia coli, GI1786861, Length=856, Percent_Identity=40.4205607476636, Blast_Score=652, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71997517, Length=202, Percent_Identity=49.009900990099, Blast_Score=212, Evalue=6e-55,
Organism=Caenorhabditis elegans, GI71997510, Length=202, Percent_Identity=49.009900990099, Blast_Score=212, Evalue=8e-55,
Organism=Caenorhabditis elegans, GI212645227, Length=309, Percent_Identity=28.4789644012945, Blast_Score=133, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6323414, Length=834, Percent_Identity=38.1294964028777, Blast_Score=548, Evalue=1e-156,
Organism=Saccharomyces cerevisiae, GI6321531, Length=398, Percent_Identity=24.6231155778894, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21355409, Length=817, Percent_Identity=34.7613219094247, Blast_Score=430, Evalue=1e-120,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 95264; Mature: 95264

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYTKHRGPLYMSYDFKNIESEIQQRCCFTIDKSENINCYVLSMFPYPSGNIHMGHIRNY
CCCCCCCCCEEEEECHHHHHHHHHHHHCEEECCCCCCCEEEEEECCCCCCCEEHHHHHHH
VIGDVIARFKRAKGFSVLHPIGWDSFGLPAENAALSYGINPELWTRNNIDSMRTQLKAIG
HHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHC
ISYNWDREITTCSEDYYKHEQSFFIDFLQNGLAYRKESWVNWDPVDNTVLANEQVIDGRG
CEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCEEECCCEEECCCC
WRSGALVERRKLSQWFLRITDYAQSLLDGLNHLPGWPEKVKLMQQRWIGRSEGVVIEFDT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECC
DCEQKLEVFSTMPHMLFGATFCAVSIDHPILQYAKAAGFAERINELTKPALQCKDAEKAG
CHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHCC
IDTGLVAKHPFLDKKLPIYVANYVLEDSGTGAVFGCPAHDQRDFEFAKLYGLPIQQVVFP
CCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCEEECCCCCCCCCHHHHHHCCCHHHEEEC
EDGSVCNLEERAYTGDGVYCNSGFLDGVRLGDAKRVMLEKLESLANCKQVTNYRLHDWGI
CCCCEECCHHHCCCCCCEEECCCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCEEECCCC
SRQRYWGCPIPIIYCTQCGIVPVDKKDLPVRLPKDVDFSKGGNPLANHPTWKHVKCHVCG
CCCCCCCCCCCEEEECCCCEEECCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEEEEC
GDAERETDTFDTFFESSWYFAAFCSRQNELNLSDSNRILPVDYYIGGIEHAVLHLLYSRF
CCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEHHHHCCHHHHHHHHHHHHH
FCRALGKCGHLQIEEPFRNLITQGMVCHTTYQDGSGNYLFPKDAEKMAAEGKKVLQGKVE
HHHHHCCCCCEEEHHHHHHHHHCCEEEEEEEECCCCCCCCCCCHHHHHHCCHHHHHHHHH
KMSKSKKNVVDPSEILKQYGADTVRLFMLSDTPPERDIEWTDCGVEGAWRYLERLWKVLG
HHHHHHCCCCCHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
SNTSISTEFNNENVSGKDLEFLSKVHKLLNGIEGDIEHCRLNCAVAKFREMSNIVFEMMK
CCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
CSASNSVINESVCILLRVMEPFIPHLTAKLWEIIGGRGMLCEQPWPSVRKALLEENFATI
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEE
AVQVDGKFCGTLRVELQCDDDKVKTEALELAQRRIKERLVKNVHYVPGKVVNIVTKA
EEEECCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
>Mature Secondary Structure
MEYTKHRGPLYMSYDFKNIESEIQQRCCFTIDKSENINCYVLSMFPYPSGNIHMGHIRNY
CCCCCCCCCEEEEECHHHHHHHHHHHHCEEECCCCCCCEEEEEECCCCCCCEEHHHHHHH
VIGDVIARFKRAKGFSVLHPIGWDSFGLPAENAALSYGINPELWTRNNIDSMRTQLKAIG
HHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHC
ISYNWDREITTCSEDYYKHEQSFFIDFLQNGLAYRKESWVNWDPVDNTVLANEQVIDGRG
CEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCEEECCCEEECCCC
WRSGALVERRKLSQWFLRITDYAQSLLDGLNHLPGWPEKVKLMQQRWIGRSEGVVIEFDT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECC
DCEQKLEVFSTMPHMLFGATFCAVSIDHPILQYAKAAGFAERINELTKPALQCKDAEKAG
CHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHCC
IDTGLVAKHPFLDKKLPIYVANYVLEDSGTGAVFGCPAHDQRDFEFAKLYGLPIQQVVFP
CCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCEEECCCCCCCCCHHHHHHCCCHHHEEEC
EDGSVCNLEERAYTGDGVYCNSGFLDGVRLGDAKRVMLEKLESLANCKQVTNYRLHDWGI
CCCCEECCHHHCCCCCCEEECCCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCEEECCCC
SRQRYWGCPIPIIYCTQCGIVPVDKKDLPVRLPKDVDFSKGGNPLANHPTWKHVKCHVCG
CCCCCCCCCCCEEEECCCCEEECCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEEEEC
GDAERETDTFDTFFESSWYFAAFCSRQNELNLSDSNRILPVDYYIGGIEHAVLHLLYSRF
CCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEHHHHCCHHHHHHHHHHHHH
FCRALGKCGHLQIEEPFRNLITQGMVCHTTYQDGSGNYLFPKDAEKMAAEGKKVLQGKVE
HHHHHCCCCCEEEHHHHHHHHHCCEEEEEEEECCCCCCCCCCCHHHHHHCCHHHHHHHHH
KMSKSKKNVVDPSEILKQYGADTVRLFMLSDTPPERDIEWTDCGVEGAWRYLERLWKVLG
HHHHHHCCCCCHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
SNTSISTEFNNENVSGKDLEFLSKVHKLLNGIEGDIEHCRLNCAVAKFREMSNIVFEMMK
CCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
CSASNSVINESVCILLRVMEPFIPHLTAKLWEIIGGRGMLCEQPWPSVRKALLEENFATI
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEE
AVQVDGKFCGTLRVELQCDDDKVKTEALELAQRRIKERLVKNVHYVPGKVVNIVTKA
EEEECCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA