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The map label for this gene is bcr [H]

Identifier: 88607041

GI number: 88607041

Start: 166520

End: 167689

Strand: Direct

Name: bcr [H]

Synonym: APH_0164

Alternate gene names: 88607041

Gene position: 166520-167689 (Clockwise)

Preceding gene: 88606875

Following gene: 88607905

Centisome position: 11.32

GC content: 41.28

Gene sequence:

>1170_bases
ATGTCTATCACAATTTGTGATATATCATACGATATGTATGCTCCAGGTCTTCCGCAAATAGGTGCGTTTTTTGGTGTAGC
CCAAACTATCGTGCAGCTTACTGTTAGCATGAATCTTCTTGGGGGTGCATTATCTGGTTTAATTTACGGGCCCTTGTCGG
ACCATTATGGTAGAAGGAGCGTAATGTTATGGGGCATTGCCATTTTCGTTATTGCGAGCATTGGGTGCTGTTTTGCCTGG
AGTATAGGTGTTCTGATAGTGCTAAGGTTCCTCCAAGGGCTAGGAGCAGGAGTAGCAGGTGTTGTGGGATTTGCTATAGT
AAATGATATATACGTTGGGGAAGCGGCGGCTAAAAATCTTTCTATAGTGAACATGGTCGTTTCTTTATCTCCCGCGGTGG
GGCCAATAATGGGTAGCTACATAGTATCATTGGGATATGGATGGAGATTATTGTTTGTACTAGTCTCGATGATTTCTGTG
CTATTACTTCTAGCCCTGATATTTGGATTGACAGAAACAATACAAAATAGACGGGAAAAGTTGTCATTATCCTCCATTCT
TGCTGAATATGGTGCACTATTTAGAAATTATAGATTCCTATGTTTCGCTCTTGTTCAGAGCCTTACAATCATGTGGGTAT
GGGCGTCAATGGCGAATTTACCGTTTGTGTTTATTGAAGGTATGCATTTGCCAGTTGAATACTACGGGTATTTAGTGGCT
ATGAGTGTAGTGTCCTATATAGCTGGGACTATAGTAAATAGGAGATTGATAGGAAAGCTGGGCATGATGCGCATGATAGT
GCTCGGTTTGCTGTCAGTAATGATACCGGATGTTATAGTGATTATATGTCACTACGCATTTCCGGCATTTCTTACTCCGC
TATTCATTGAGATGATTTGGTTGCCATCTTCCTTTGGTATAGCATTTATCACAAGTAATAGTGTTGCGATTGCTTTTGGT
GAAGTTAGGGATAAGGGAGCGGCATCGGCATTTATATCGTTTGGGCAAACAATTTTTGGTGCTTGTGGTATATATATCGT
GGGCAAGTTTTACAATGGCACCATTGTTCCGGTATCTGTGCTGCCTATAGTGTGTTGTTTATTGGGTATGACTATTATTT
GGCTTCTCACAAAGAGAGAAGGCATGTTCCCAAGAGAAAAGACAGTATGA

Upstream 100 bases:

>100_bases
CCGAATTTGCTCATGGTATGTAAGGAAGAGCAGTTGTTTTTATAGGGGTTTTTTGGTTATGCTCGTGCTTCGAGAGACTG
TGGTATTGTTTCTTGTGATA

Downstream 100 bases:

>100_bases
TCTCATGCAATGTTGCGTTGCGAGGTCCAATCAAAGCAAATTCGATTTGCCGAGACTAAAGTTCTAGAGTTACGACTCTC
TATAGCAAACTTACTAAACC

Product: bicyclomycin resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MSITICDISYDMYAPGLPQIGAFFGVAQTIVQLTVSMNLLGGALSGLIYGPLSDHYGRRSVMLWGIAIFVIASIGCCFAW
SIGVLIVLRFLQGLGAGVAGVVGFAIVNDIYVGEAAAKNLSIVNMVVSLSPAVGPIMGSYIVSLGYGWRLLFVLVSMISV
LLLLALIFGLTETIQNRREKLSLSSILAEYGALFRNYRFLCFALVQSLTIMWVWASMANLPFVFIEGMHLPVEYYGYLVA
MSVVSYIAGTIVNRRLIGKLGMMRMIVLGLLSVMIPDVIVIICHYAFPAFLTPLFIEMIWLPSSFGIAFITSNSVAIAFG
EVRDKGAASAFISFGQTIFGACGIYIVGKFYNGTIVPVSVLPIVCCLLGMTIIWLLTKREGMFPREKTV

Sequences:

>Translated_389_residues
MSITICDISYDMYAPGLPQIGAFFGVAQTIVQLTVSMNLLGGALSGLIYGPLSDHYGRRSVMLWGIAIFVIASIGCCFAW
SIGVLIVLRFLQGLGAGVAGVVGFAIVNDIYVGEAAAKNLSIVNMVVSLSPAVGPIMGSYIVSLGYGWRLLFVLVSMISV
LLLLALIFGLTETIQNRREKLSLSSILAEYGALFRNYRFLCFALVQSLTIMWVWASMANLPFVFIEGMHLPVEYYGYLVA
MSVVSYIAGTIVNRRLIGKLGMMRMIVLGLLSVMIPDVIVIICHYAFPAFLTPLFIEMIWLPSSFGIAFITSNSVAIAFG
EVRDKGAASAFISFGQTIFGACGIYIVGKFYNGTIVPVSVLPIVCCLLGMTIIWLLTKREGMFPREKTV
>Mature_388_residues
SITICDISYDMYAPGLPQIGAFFGVAQTIVQLTVSMNLLGGALSGLIYGPLSDHYGRRSVMLWGIAIFVIASIGCCFAWS
IGVLIVLRFLQGLGAGVAGVVGFAIVNDIYVGEAAAKNLSIVNMVVSLSPAVGPIMGSYIVSLGYGWRLLFVLVSMISVL
LLLALIFGLTETIQNRREKLSLSSILAEYGALFRNYRFLCFALVQSLTIMWVWASMANLPFVFIEGMHLPVEYYGYLVAM
SVVSYIAGTIVNRRLIGKLGMMRMIVLGLLSVMIPDVIVIICHYAFPAFLTPLFIEMIWLPSSFGIAFITSNSVAIAFGE
VRDKGAASAFISFGQTIFGACGIYIVGKFYNGTIVPVSVLPIVCCLLGMTIIWLLTKREGMFPREKTV

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=267, Percent_Identity=34.4569288389513, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1790146, Length=232, Percent_Identity=30.6034482758621, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI48994889, Length=249, Percent_Identity=30.9236947791165, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787065, Length=349, Percent_Identity=25.7879656160458, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790794, Length=275, Percent_Identity=24.3636363636364, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87082312, Length=201, Percent_Identity=30.8457711442786, Blast_Score=72, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6319480, Length=179, Percent_Identity=29.0502793296089, Blast_Score=70, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6324264, Length=141, Percent_Identity=31.9148936170213, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319517, Length=178, Percent_Identity=30.8988764044944, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42245; Mature: 42114

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITICDISYDMYAPGLPQIGAFFGVAQTIVQLTVSMNLLGGALSGLIYGPLSDHYGRRS
CEEEEEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHCCCCHH
VMLWGIAIFVIASIGCCFAWSIGVLIVLRFLQGLGAGVAGVVGFAIVNDIYVGEAAAKNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHCCH
SIVNMVVSLSPAVGPIMGSYIVSLGYGWRLLFVLVSMISVLLLLALIFGLTETIQNRREK
HHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSLSSILAEYGALFRNYRFLCFALVQSLTIMWVWASMANLPFVFIEGMHLPVEYYGYLVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHH
MSVVSYIAGTIVNRRLIGKLGMMRMIVLGLLSVMIPDVIVIICHYAFPAFLTPLFIEMIW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPSSFGIAFITSNSVAIAFGEVRDKGAASAFISFGQTIFGACGIYIVGKFYNGTIVPVSV
CCCCCCEEEEECCCEEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEHHHH
LPIVCCLLGMTIIWLLTKREGMFPREKTV
HHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SITICDISYDMYAPGLPQIGAFFGVAQTIVQLTVSMNLLGGALSGLIYGPLSDHYGRRS
EEEEEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHCCCCHH
VMLWGIAIFVIASIGCCFAWSIGVLIVLRFLQGLGAGVAGVVGFAIVNDIYVGEAAAKNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHCCH
SIVNMVVSLSPAVGPIMGSYIVSLGYGWRLLFVLVSMISVLLLLALIFGLTETIQNRREK
HHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSLSSILAEYGALFRNYRFLCFALVQSLTIMWVWASMANLPFVFIEGMHLPVEYYGYLVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHH
MSVVSYIAGTIVNRRLIGKLGMMRMIVLGLLSVMIPDVIVIICHYAFPAFLTPLFIEMIW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPSSFGIAFITSNSVAIAFGEVRDKGAASAFISFGQTIFGACGIYIVGKFYNGTIVPVSV
CCCCCCEEEEECCCEEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEHHHH
LPIVCCLLGMTIIWLLTKREGMFPREKTV
HHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]