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The map label for this gene is gltX

Identifier: 88606767

GI number: 88606767

Start: 425394

End: 426800

Strand: Reverse

Name: gltX

Synonym: APH_0408

Alternate gene names: 88606767

Gene position: 426800-425394 (Counterclockwise)

Preceding gene: 88607827

Following gene: 88607492

Centisome position: 29.01

GC content: 41.79

Gene sequence:

>1407_bases
GTGCGCGTCGTTACGAGATTTGCTCCATCTCCCACTGGAAGCTTGCATTTAGGAGGAGCTAGAACTGCGCTTTTTAACTG
GTTATTTGCCAGACATCACAAGGGAAAATTTTTGCTGCGCATGGAAGATACCGACAAAAAACGGTCATCCGACGTGGTTG
TACAGTCTATAATCGATGACATGTCATGGCTAGGCCTCCAACACGATGGCGATATTGTAGTACAATCTTCTAGAGCCGCT
AGACATGTCGCAGTAGCGAGAGAGTTAGTAGAATTAGGTAGAGCTTATCGGTGCTATTGTTCGGAAGATGAAGTCAATGA
GCAAAAGTTACAGTCTGAAGGTACTGGTAAATATTTCAGACACGTGTGTCCCTGGAAACATCTGAATAGCACTGGTGATC
TTCCCAACAAACCATATGTCGTAAGGCTCAAAAGCCCTGAAAATACCACAATAGAGTTTCTCGATGGTGTATATGGGAAA
ATATCCGTAAAAAGCGATCAGATAGACGATATGGTGATATTGCGCTCTGATGGCACGCCTACATATCTTCTGGCCGTTGT
AGTTGATGACCATGATATGGAAATTACGCACATAATACGGGGTTCTGATCACATCACGAATACTGTAAAGCAAATCGTGC
TAGCTGAAGCTATGAGCTGGGTCAGTCCGAAATTTTTTCACATCCCATTAATTCACGATGAAAATGGAGCCAAGCTATCT
AAGCGCAATCGTGCTCCTGGAATACACGAATACAAAGAACAGGGGTTTCTGCCTGAAGCACTGTGCAACTATCTGCTGCG
CATGGGATGGAGTTACCAAAATAAGGAAATTGTGTCTATGCAAGAAGCAATAGCGCTATTTTCCATGGAAGATGTAGGAG
TTTCATGTTCGTGTTTGGACTACAAAAAATTGGTGTTTTTAAACCATCATTACATGGGATCAAAAAGCGAATCAGAAATT
CTAGATTTGTTGCTACCAATCCTTGAAGAAAAGCTGGGTGGTAGAATCTCCGAAGAAAAACTCTCACGTTTATCCTTGGG
AATAAAACAACTGGTAGAACGCGCAAAAACCTTAACTGACCTCGCTGAAGACTCACTTTTTTATGTTCAAGACGTGGAAA
TCAACATCAATCCAGAAGCTGTAGAAACAATTCAAAACAGCAAAAAGTTCCTTGCAGAATTGCTGGAAAGTATGTCAGGA
ATCCACCCAGATATGTGGAAGAAAACCCACCTATCTTCGCAGATTAAGGAATTCTCTAAAACACGTAATCTGGCTATGAG
TGATGTATATCATTTCCTCAGAGCATCTATAACGGGTAGACTGCAATCCCCTAACATTTCGGAAGTTATGGAGATATTAG
GACAAGAAATGTGTATCAACAGGATGCTTTCAGCACAGGAAATCTGA

Upstream 100 bases:

>100_bases
CGTCTCTGTGTAGTACTTTAATTCTCCTGCTAGTGCGTTGCTATTGGTTTTTTTTCGTTGTACCATAACCCCTCTATGGA
TTTTTAACAGGTCTACAGCA

Downstream 100 bases:

>100_bases
CACTTCACCGTATTGGGCGAAGCTTGAGTTTTGGTACCGCATGTATCATGTACACTTGCAGTATTAGTTGTACTAGAGGC
TTTATTAGAATCGTATCGGC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 2; GluRS 2

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MRVVTRFAPSPTGSLHLGGARTALFNWLFARHHKGKFLLRMEDTDKKRSSDVVVQSIIDDMSWLGLQHDGDIVVQSSRAA
RHVAVARELVELGRAYRCYCSEDEVNEQKLQSEGTGKYFRHVCPWKHLNSTGDLPNKPYVVRLKSPENTTIEFLDGVYGK
ISVKSDQIDDMVILRSDGTPTYLLAVVVDDHDMEITHIIRGSDHITNTVKQIVLAEAMSWVSPKFFHIPLIHDENGAKLS
KRNRAPGIHEYKEQGFLPEALCNYLLRMGWSYQNKEIVSMQEAIALFSMEDVGVSCSCLDYKKLVFLNHHYMGSKSESEI
LDLLLPILEEKLGGRISEEKLSRLSLGIKQLVERAKTLTDLAEDSLFYVQDVEININPEAVETIQNSKKFLAELLESMSG
IHPDMWKKTHLSSQIKEFSKTRNLAMSDVYHFLRASITGRLQSPNISEVMEILGQEMCINRMLSAQEI

Sequences:

>Translated_468_residues
MRVVTRFAPSPTGSLHLGGARTALFNWLFARHHKGKFLLRMEDTDKKRSSDVVVQSIIDDMSWLGLQHDGDIVVQSSRAA
RHVAVARELVELGRAYRCYCSEDEVNEQKLQSEGTGKYFRHVCPWKHLNSTGDLPNKPYVVRLKSPENTTIEFLDGVYGK
ISVKSDQIDDMVILRSDGTPTYLLAVVVDDHDMEITHIIRGSDHITNTVKQIVLAEAMSWVSPKFFHIPLIHDENGAKLS
KRNRAPGIHEYKEQGFLPEALCNYLLRMGWSYQNKEIVSMQEAIALFSMEDVGVSCSCLDYKKLVFLNHHYMGSKSESEI
LDLLLPILEEKLGGRISEEKLSRLSLGIKQLVERAKTLTDLAEDSLFYVQDVEININPEAVETIQNSKKFLAELLESMSG
IHPDMWKKTHLSSQIKEFSKTRNLAMSDVYHFLRASITGRLQSPNISEVMEILGQEMCINRMLSAQEI
>Mature_468_residues
MRVVTRFAPSPTGSLHLGGARTALFNWLFARHHKGKFLLRMEDTDKKRSSDVVVQSIIDDMSWLGLQHDGDIVVQSSRAA
RHVAVARELVELGRAYRCYCSEDEVNEQKLQSEGTGKYFRHVCPWKHLNSTGDLPNKPYVVRLKSPENTTIEFLDGVYGK
ISVKSDQIDDMVILRSDGTPTYLLAVVVDDHDMEITHIIRGSDHITNTVKQIVLAEAMSWVSPKFFHIPLIHDENGAKLS
KRNRAPGIHEYKEQGFLPEALCNYLLRMGWSYQNKEIVSMQEAIALFSMEDVGVSCSCLDYKKLVFLNHHYMGSKSESEI
LDLLLPILEEKLGGRISEEKLSRLSLGIKQLVERAKTLTDLAEDSLFYVQDVEININPEAVETIQNSKKFLAELLESMSG
IHPDMWKKTHLSSQIKEFSKTRNLAMSDVYHFLRASITGRLQSPNISEVMEILGQEMCINRMLSAQEI

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=483, Percent_Identity=30.2277432712215, Blast_Score=202, Evalue=7e-52,
Organism=Escherichia coli, GI1788743, Length=475, Percent_Identity=36, Blast_Score=318, Evalue=5e-88,
Organism=Escherichia coli, GI226510925, Length=246, Percent_Identity=36.9918699186992, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1786895, Length=115, Percent_Identity=33.0434782608696, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=491, Percent_Identity=31.1608961303462, Blast_Score=212, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6324540, Length=483, Percent_Identity=32.5051759834368, Blast_Score=241, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6324742, Length=123, Percent_Identity=30.8943089430894, Blast_Score=76, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6321192, Length=121, Percent_Identity=31.404958677686, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24665321, Length=495, Percent_Identity=32.7272727272727, Blast_Score=239, Evalue=2e-63,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE2_ANAPZ (Q2GKT8)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505013.1
- ProteinModelPortal:   Q2GKT8
- SMR:   Q2GKT8
- STRING:   Q2GKT8
- GeneID:   3930482
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0408
- NMPDR:   fig|212042.5.peg.396
- TIGR:   APH_0408
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   NGSGIRD
- PhylomeDB:   Q2GKT8
- ProtClustDB:   PRK01406
- BioCyc:   APHA212042:APH_0408-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 53153; Mature: 53153

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 241-241

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVVTRFAPSPTGSLHLGGARTALFNWLFARHHKGKFLLRMEDTDKKRSSDVVVQSIIDD
CCEEEECCCCCCCCEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHH
MSWLGLQHDGDIVVQSSRAARHVAVARELVELGRAYRCYCSEDEVNEQKLQSEGTGKYFR
HHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCCCCHHHH
HVCPWKHLNSTGDLPNKPYVVRLKSPENTTIEFLDGVYGKISVKSDQIDDMVILRSDGTP
HCCCHHHCCCCCCCCCCCEEEEECCCCCCHHHHHCCCCCEEEECCCCCCCEEEEECCCCC
TYLLAVVVDDHDMEITHIIRGSDHITNTVKQIVLAEAMSWVSPKFFHIPLIHDENGAKLS
EEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCH
KRNRAPGIHEYKEQGFLPEALCNYLLRMGWSYQNKEIVSMQEAIALFSMEDVGVSCSCLD
HCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEC
YKKLVFLNHHYMGSKSESEILDLLLPILEEKLGGRISEEKLSRLSLGIKQLVERAKTLTD
HHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LAEDSLFYVQDVEININPEAVETIQNSKKFLAELLESMSGIHPDMWKKTHLSSQIKEFSK
HHCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
TRNLAMSDVYHFLRASITGRLQSPNISEVMEILGQEMCINRMLSAQEI
HHCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRVVTRFAPSPTGSLHLGGARTALFNWLFARHHKGKFLLRMEDTDKKRSSDVVVQSIIDD
CCEEEECCCCCCCCEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHH
MSWLGLQHDGDIVVQSSRAARHVAVARELVELGRAYRCYCSEDEVNEQKLQSEGTGKYFR
HHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCCCCHHHH
HVCPWKHLNSTGDLPNKPYVVRLKSPENTTIEFLDGVYGKISVKSDQIDDMVILRSDGTP
HCCCHHHCCCCCCCCCCCEEEEECCCCCCHHHHHCCCCCEEEECCCCCCCEEEEECCCCC
TYLLAVVVDDHDMEITHIIRGSDHITNTVKQIVLAEAMSWVSPKFFHIPLIHDENGAKLS
EEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCH
KRNRAPGIHEYKEQGFLPEALCNYLLRMGWSYQNKEIVSMQEAIALFSMEDVGVSCSCLD
HCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEC
YKKLVFLNHHYMGSKSESEILDLLLPILEEKLGGRISEEKLSRLSLGIKQLVERAKTLTD
HHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LAEDSLFYVQDVEININPEAVETIQNSKKFLAELLESMSGIHPDMWKKTHLSSQIKEFSK
HHCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
TRNLAMSDVYHFLRASITGRLQSPNISEVMEILGQEMCINRMLSAQEI
HHCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA