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The map label for this gene is ksgA

Identifier: 88606758

GI number: 88606758

Start: 649502

End: 650311

Strand: Reverse

Name: ksgA

Synonym: APH_0622

Alternate gene names: 88606758

Gene position: 650311-649502 (Counterclockwise)

Preceding gene: 88607093

Following gene: 88607303

Centisome position: 44.2

GC content: 36.54

Gene sequence:

>810_bases
ATGAAAATTACTAAATACAGGACGAAAAAATCTTTAGGGCAGTGTTTCATACTAGATCCTGCTATCGCGGAAAAAATTGT
TTCATATGCAGGATGCTTGGAACAATATAACGTAATAGAAGTCGGACCAGGGCTTGGAATAATGACTCAATCAATCCTTA
ACAAAGAGGTACGTAGACTAACCGCTATTGAGAAGGATAGAAGATTATCAAACATACACAGCAAACTAAAAGAAGCACAT
GCTGAATATGATTGCATCTTTGAAGATATTTTGGATGTCAACATCGAACAGCTGCTATCGCAATCGCCCCTCAAAATGAT
ATCCAACCTCCCGTACAATATATCTGTAATTTTGCTTCTAAAACTCCTTCCCTATATACATCGCTTCGAGAAGCTCATTC
TTATGTTCCAAAAAGAAGTAGCTGATAGGATCGTAGCTCAGCCAAATACGAAAAGCTATTCTATTCTGTCTATTCTTGTG
CAGCTGCTATGCGATGTTAGAAAAGTTGAAGATTTCCCACCAGAAATTTTCTCCCCATCTCCTAAAGTATATTCTTCAGT
TATAGAGATAACTCCCTTATTATCTCCTAGATTCTCTGTAGATAATTCATACTTTGCACAAGTATTAAAAAAGCTATTCC
ACTGCCGACGAAAAACAATTCGCAACTCGTTAAAGTCATGTATAAAAGATGCTGATGCATTATTTATTGGATGTAATATT
GACCCTAATGCAAGAGCAGAATCCCTGACTATAGAGCAATTGTGCTCACTAACAAATGCCTTAAAAGCGCGCAATATCAA
TATAATCTGA

Upstream 100 bases:

>100_bases
AATCCTATAACAGCTAGCGGTAGCTATGATATACATGTTAGGATAAAAGTCAATACACTAAAACATGACTATTTATGGAG
ATATGCATGAGAATTAATGT

Downstream 100 bases:

>100_bases
CCATACCAACAGAAACTAGAAATGATAATCTGTCAATACAGCGTTATTTATGGTTTTATGCCCGATATTACTTCCGGATA
AAAACACGATACAATTGATA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MKITKYRTKKSLGQCFILDPAIAEKIVSYAGCLEQYNVIEVGPGLGIMTQSILNKEVRRLTAIEKDRRLSNIHSKLKEAH
AEYDCIFEDILDVNIEQLLSQSPLKMISNLPYNISVILLLKLLPYIHRFEKLILMFQKEVADRIVAQPNTKSYSILSILV
QLLCDVRKVEDFPPEIFSPSPKVYSSVIEITPLLSPRFSVDNSYFAQVLKKLFHCRRKTIRNSLKSCIKDADALFIGCNI
DPNARAESLTIEQLCSLTNALKARNINII

Sequences:

>Translated_269_residues
MKITKYRTKKSLGQCFILDPAIAEKIVSYAGCLEQYNVIEVGPGLGIMTQSILNKEVRRLTAIEKDRRLSNIHSKLKEAH
AEYDCIFEDILDVNIEQLLSQSPLKMISNLPYNISVILLLKLLPYIHRFEKLILMFQKEVADRIVAQPNTKSYSILSILV
QLLCDVRKVEDFPPEIFSPSPKVYSSVIEITPLLSPRFSVDNSYFAQVLKKLFHCRRKTIRNSLKSCIKDADALFIGCNI
DPNARAESLTIEQLCSLTNALKARNINII
>Mature_269_residues
MKITKYRTKKSLGQCFILDPAIAEKIVSYAGCLEQYNVIEVGPGLGIMTQSILNKEVRRLTAIEKDRRLSNIHSKLKEAH
AEYDCIFEDILDVNIEQLLSQSPLKMISNLPYNISVILLLKLLPYIHRFEKLILMFQKEVADRIVAQPNTKSYSILSILV
QLLCDVRKVEDFPPEIFSPSPKVYSSVIEITPLLSPRFSVDNSYFAQVLKKLFHCRRKTIRNSLKSCIKDADALFIGCNI
DPNARAESLTIEQLCSLTNALKARNINII

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=287, Percent_Identity=32.0557491289199, Blast_Score=140, Evalue=8e-34,
Organism=Homo sapiens, GI7657198, Length=219, Percent_Identity=32.4200913242009, Blast_Score=91, Evalue=9e-19,
Organism=Escherichia coli, GI1786236, Length=260, Percent_Identity=34.6153846153846, Blast_Score=150, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI25141369, Length=291, Percent_Identity=28.1786941580756, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI25146882, Length=212, Percent_Identity=32.0754716981132, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324989, Length=225, Percent_Identity=29.7777777777778, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21357273, Length=280, Percent_Identity=30, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21358017, Length=219, Percent_Identity=32.4200913242009, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_ANAPZ (Q2GK91)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505210.1
- ProteinModelPortal:   Q2GK91
- SMR:   Q2GK91
- STRING:   Q2GK91
- GeneID:   3930473
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0622
- NMPDR:   fig|212042.5.peg.611
- TIGR:   APH_0622
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   RAENLTP
- PhylomeDB:   Q2GK91
- ProtClustDB:   PRK00274
- BioCyc:   APHA212042:APH_0622-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30615; Mature: 30615

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 17-17 BINDING 42-42 BINDING 64-64 BINDING 89-89 BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITKYRTKKSLGQCFILDPAIAEKIVSYAGCLEQYNVIEVGPGLGIMTQSILNKEVRRL
CCCCHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHH
TAIEKDRRLSNIHSKLKEAHAEYDCIFEDILDVNIEQLLSQSPLKMISNLPYNISVILLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHCCCCCHHHHHHH
KLLPYIHRFEKLILMFQKEVADRIVAQPNTKSYSILSILVQLLCDVRKVEDFPPEIFSPS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCC
PKVYSSVIEITPLLSPRFSVDNSYFAQVLKKLFHCRRKTIRNSLKSCIKDADALFIGCNI
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
DPNARAESLTIEQLCSLTNALKARNINII
CCCCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKITKYRTKKSLGQCFILDPAIAEKIVSYAGCLEQYNVIEVGPGLGIMTQSILNKEVRRL
CCCCHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHH
TAIEKDRRLSNIHSKLKEAHAEYDCIFEDILDVNIEQLLSQSPLKMISNLPYNISVILLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHCCCCCHHHHHHH
KLLPYIHRFEKLILMFQKEVADRIVAQPNTKSYSILSILVQLLCDVRKVEDFPPEIFSPS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCC
PKVYSSVIEITPLLSPRFSVDNSYFAQVLKKLFHCRRKTIRNSLKSCIKDADALFIGCNI
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
DPNARAESLTIEQLCSLTNALKARNINII
CCCCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA