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The map label for this gene is miaA

Identifier: 88606708

GI number: 88606708

Start: 445920

End: 446858

Strand: Reverse

Name: miaA

Synonym: APH_0431

Alternate gene names: 88606708

Gene position: 446858-445920 (Counterclockwise)

Preceding gene: 88606769

Following gene: 88607906

Centisome position: 30.37

GC content: 43.02

Gene sequence:

>939_bases
ATGGAGAAAGAAATAGTCGTAGTTACAGGACCAACAGCGTCCGGCAAGTCTGCAGCTTGTGATATTATTGCCGATGATCT
CAACTGTAGAGTGATAAATTGTGACTCAAAACAAATTTACCACGGTGTGCCAATTCTCACTGATCAACCTGTATCTGTAC
GCGACATACATAAGTTGTATGGATATGTTGCGCCAACACAAAATTATTCTGCAGGATTATGGTTACAGGACGTAAAAAGA
GAGGTACAGCAGGCTTGGGATGACAATGTGCTACCAGTCATAACAGGCGGCAGCGGGATGTATATAAACAGCCTCGTCAA
CGGCATATCTGACATACCGGCAATAGATCCTGAAGTCCGCAAAACCGCAAGAAATCTTTTAGAATCTCTCGGGAACGCTG
CATTTTATGAAGCACTTGTTGCAAGAGATAGCAATGCTGCACGTCTACATCATAACAATACCCATCAGATATTACGCGCA
TTTGAGGTTTTGGAGCAAACTGGAACGTCGATATTCACTTGGTGGGAGCGTTCACCGCGTATTAAGCCTTTTGAGAATTG
CGAAGTCTTAGTCTTGTTACCTCCTCGCAACAAACTATACGACAAGATTAATCGAAGATTTCTCACAATGATGCAAACGG
ATGCAATATCTGAGGTAAAGTACTTAATGTCACTGAATCTGCCTATGCATGCGCCCGTTATGAAGGCACATGGAGCTCCT
GAAATTGTACAATACTTGCAAGGTAAAATAGAGTTTATGGAAGCTGTGGAAATAGCTCAGAAGAATACGCGTCATTATGC
AAAAAGGCAATGTACGTGGTTCAGAACCCAGTTGCCTTGTGATACACGCTTCTTCAGCTCTCAGAATGAAATCATAGATC
ACGTACTTGCGCAATACGCTTCAAAGTATCCGAGAACCCACACACGGCCCTGTAATTAG

Upstream 100 bases:

>100_bases
AGAACAATACATACTGTATATCATCGTACAATTGTGGTATCCTGCAAGTTAGTTACACGGAATTGTACTCTCAGAACAAA
ATTTGAGATAATTAATAGAC

Downstream 100 bases:

>100_bases
CACATAACATGCCATCTGTAACAATGTTTGAAAGTATAGCTGAAGAGCATAAATCATAACGCATCAAAGTATCAGAACGT
TATCGACTACATATAACCTG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MEKEIVVVTGPTASGKSAACDIIADDLNCRVINCDSKQIYHGVPILTDQPVSVRDIHKLYGYVAPTQNYSAGLWLQDVKR
EVQQAWDDNVLPVITGGSGMYINSLVNGISDIPAIDPEVRKTARNLLESLGNAAFYEALVARDSNAARLHHNNTHQILRA
FEVLEQTGTSIFTWWERSPRIKPFENCEVLVLLPPRNKLYDKINRRFLTMMQTDAISEVKYLMSLNLPMHAPVMKAHGAP
EIVQYLQGKIEFMEAVEIAQKNTRHYAKRQCTWFRTQLPCDTRFFSSQNEIIDHVLAQYASKYPRTHTRPCN

Sequences:

>Translated_312_residues
MEKEIVVVTGPTASGKSAACDIIADDLNCRVINCDSKQIYHGVPILTDQPVSVRDIHKLYGYVAPTQNYSAGLWLQDVKR
EVQQAWDDNVLPVITGGSGMYINSLVNGISDIPAIDPEVRKTARNLLESLGNAAFYEALVARDSNAARLHHNNTHQILRA
FEVLEQTGTSIFTWWERSPRIKPFENCEVLVLLPPRNKLYDKINRRFLTMMQTDAISEVKYLMSLNLPMHAPVMKAHGAP
EIVQYLQGKIEFMEAVEIAQKNTRHYAKRQCTWFRTQLPCDTRFFSSQNEIIDHVLAQYASKYPRTHTRPCN
>Mature_312_residues
MEKEIVVVTGPTASGKSAACDIIADDLNCRVINCDSKQIYHGVPILTDQPVSVRDIHKLYGYVAPTQNYSAGLWLQDVKR
EVQQAWDDNVLPVITGGSGMYINSLVNGISDIPAIDPEVRKTARNLLESLGNAAFYEALVARDSNAARLHHNNTHQILRA
FEVLEQTGTSIFTWWERSPRIKPFENCEVLVLLPPRNKLYDKINRRFLTMMQTDAISEVKYLMSLNLPMHAPVMKAHGAP
EIVQYLQGKIEFMEAVEIAQKNTRHYAKRQCTWFRTQLPCDTRFFSSQNEIIDHVLAQYASKYPRTHTRPCN

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=326, Percent_Identity=21.7791411042945, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790613, Length=275, Percent_Identity=29.8181818181818, Blast_Score=122, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6324848, Length=299, Percent_Identity=24.4147157190635, Blast_Score=98, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_ANAPZ (Q2GKR7)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505034.1
- ProteinModelPortal:   Q2GKR7
- SMR:   Q2GKR7
- STRING:   Q2GKR7
- GeneID:   3929983
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0431
- NMPDR:   fig|212042.5.peg.418
- TIGR:   APH_0431
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   CLSK749553
- BioCyc:   APHA212042:APH_0431-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35474; Mature: 35474

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKEIVVVTGPTASGKSAACDIIADDLNCRVINCDSKQIYHGVPILTDQPVSVRDIHKLY
CCCCEEEEECCCCCCCCCEEEEEECCCCEEEEECCCCHHHCCCCCCCCCCCCHHHHHHHH
GYVAPTQNYSAGLWLQDVKREVQQAWDDNVLPVITGGSGMYINSLVNGISDIPAIDPEVR
HHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEHHHHHHHHHCCCCCCHHHH
KTARNLLESLGNAAFYEALVARDSNAARLHHNNTHQILRAFEVLEQTGTSIFTWWERSPR
HHHHHHHHHHCCHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCC
IKPFENCEVLVLLPPRNKLYDKINRRFLTMMQTDAISEVKYLMSLNLPMHAPVMKAHGAP
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCH
EIVQYLQGKIEFMEAVEIAQKNTRHYAKRQCTWFRTQLPCDTRFFSSQNEIIDHVLAQYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHH
SKYPRTHTRPCN
HHCCCCCCCCCC
>Mature Secondary Structure
MEKEIVVVTGPTASGKSAACDIIADDLNCRVINCDSKQIYHGVPILTDQPVSVRDIHKLY
CCCCEEEEECCCCCCCCCEEEEEECCCCEEEEECCCCHHHCCCCCCCCCCCCHHHHHHHH
GYVAPTQNYSAGLWLQDVKREVQQAWDDNVLPVITGGSGMYINSLVNGISDIPAIDPEVR
HHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEHHHHHHHHHCCCCCCHHHH
KTARNLLESLGNAAFYEALVARDSNAARLHHNNTHQILRAFEVLEQTGTSIFTWWERSPR
HHHHHHHHHHCCHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCC
IKPFENCEVLVLLPPRNKLYDKINRRFLTMMQTDAISEVKYLMSLNLPMHAPVMKAHGAP
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCH
EIVQYLQGKIEFMEAVEIAQKNTRHYAKRQCTWFRTQLPCDTRFFSSQNEIIDHVLAQYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHH
SKYPRTHTRPCN
HHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA