Click here to switch to the map view.

The map label for this gene is csgA [H]

Identifier: 87200972

GI number: 87200972

Start: 3177345

End: 3178076

Strand: Direct

Name: csgA [H]

Synonym: Saro_2960

Alternate gene names: 87200972

Gene position: 3177345-3178076 (Clockwise)

Preceding gene: 87200968

Following gene: 87200973

Centisome position: 89.21

GC content: 67.35

Gene sequence:

>732_bases
ATGGACATGGAATCGGGCAAGGGCATGCGCTGTGCCGTCTTCGGCGCAAGCGGCGGCATCGGACGGGCCCTCGCCGACCT
CCTGGCAGCGCGAAAGGACGTGGCCGAAGTCCATGCGCTGGCAAGGCGCACCATTCCCGCAGGCACGAAGGTCCGCCCCC
ACCGCTTCGACCTCGAGGACGAAGACTCCATTGCAAGGGCCTGCGGCGAGATCGGCGCCCCGCTGGACCTGGTGATCGTC
GCGACGGGCCGCCTGGTACGCGACAACGGCGACGGTCCAGAGAAATCCTGGCGGGCGATCAGCCGGGACGGCATGGCCGA
ACTGTTCGCGATCAACGCCATCGGTCCTGCGGTGATCGCGCGTCACACCTTGCCTCTCCTGCGCCGGGAAGGCCGCCCCG
TCTTCGCGGCGATCTCGGCCCGGGTTGGCTCGATCTCGGACAACCGCCTCGGCGGCTGGCATTCTTACCGCGCGTCCAAG
GCGGCGCTGAACATGCTGGTACGAAACTTCGCAATCGAACTTGCGCGGACCAATCCTTCGGCCACCGCCGTCACGCTCCA
CCCCGGTACGGTCGACACGCCCCTCTCGCGTCCGTTCCAGCGCAGTGTACCGGCAGACAGGCTGTTCTCGCCCGAACAGT
CAGCAGCGCACCTCCTTGCGGTGATCGATCGCCTCACTCCCGCCGACAGCGGCCAGCTCTTTGCATGGGACGGGTCCGCG
ATCCCTTTCTGA

Upstream 100 bases:

>100_bases
GATGTCGCGCGCTGCCGCGCAAGCTGTCTTATACACGCAACACAGCGCCGCGCCTTCGTCCATATCGACGGACCGTTGTC
CGATGTCCATGAACGACAGC

Downstream 100 bases:

>100_bases
GAATGCCCACCATTCGAATTTCACGCAGTGTAACTTTTGGAAAGACTGTCGCTGGTCGGTACTGCGGCAGGTGCTAGTCT
CCCGCCAAGACCCGGAAAGC

Product: short-chain dehydrogenase/reductase SDR

Products: 2,3-Dihydroxybenzoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: C signal [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MDMESGKGMRCAVFGASGGIGRALADLLAARKDVAEVHALARRTIPAGTKVRPHRFDLEDEDSIARACGEIGAPLDLVIV
ATGRLVRDNGDGPEKSWRAISRDGMAELFAINAIGPAVIARHTLPLLRREGRPVFAAISARVGSISDNRLGGWHSYRASK
AALNMLVRNFAIELARTNPSATAVTLHPGTVDTPLSRPFQRSVPADRLFSPEQSAAHLLAVIDRLTPADSGQLFAWDGSA
IPF

Sequences:

>Translated_243_residues
MDMESGKGMRCAVFGASGGIGRALADLLAARKDVAEVHALARRTIPAGTKVRPHRFDLEDEDSIARACGEIGAPLDLVIV
ATGRLVRDNGDGPEKSWRAISRDGMAELFAINAIGPAVIARHTLPLLRREGRPVFAAISARVGSISDNRLGGWHSYRASK
AALNMLVRNFAIELARTNPSATAVTLHPGTVDTPLSRPFQRSVPADRLFSPEQSAAHLLAVIDRLTPADSGQLFAWDGSA
IPF
>Mature_243_residues
MDMESGKGMRCAVFGASGGIGRALADLLAARKDVAEVHALARRTIPAGTKVRPHRFDLEDEDSIARACGEIGAPLDLVIV
ATGRLVRDNGDGPEKSWRAISRDGMAELFAINAIGPAVIARHTLPLLRREGRPVFAAISARVGSISDNRLGGWHSYRASK
AALNMLVRNFAIELARTNPSATAVTLHPGTVDTPLSRPFQRSVPADRLFSPEQSAAHLLAVIDRLTPADSGQLFAWDGSA
IPF

Specific function: Cell-cell signaling protein required for fruiting body morphogenesis. Necessary for cellular aggregation, for spore differentiation, and for gene expression that is initiated after 6 hour of starvation [H]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To yeast YKL071W [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.3.1.28 [C]

Molecular weight: Translated: 25947; Mature: 25947

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMESGKGMRCAVFGASGGIGRALADLLAARKDVAEVHALARRTIPAGTKVRPHRFDLED
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
EDSIARACGEIGAPLDLVIVATGRLVRDNGDGPEKSWRAISRDGMAELFAINAIGPAVIA
HHHHHHHHHHHCCCEEEEEEECCEEEECCCCCHHHHHHHHHCCCHHHEEEECCCCHHHHH
RHTLPLLRREGRPVFAAISARVGSISDNRLGGWHSYRASKAALNMLVRNFAIELARTNPS
HHHHHHHHHCCCCEEEEEHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ATAVTLHPGTVDTPLSRPFQRSVPADRLFSPEQSAAHLLAVIDRLTPADSGQLFAWDGSA
CEEEEECCCCCCCCCCCCHHHCCCHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCC
IPF
CCC
>Mature Secondary Structure
MDMESGKGMRCAVFGASGGIGRALADLLAARKDVAEVHALARRTIPAGTKVRPHRFDLED
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
EDSIARACGEIGAPLDLVIVATGRLVRDNGDGPEKSWRAISRDGMAELFAINAIGPAVIA
HHHHHHHHHHHCCCEEEEEEECCEEEECCCCCHHHHHHHHHCCCHHHEEEECCCCHHHHH
RHTLPLLRREGRPVFAAISARVGSISDNRLGGWHSYRASKAALNMLVRNFAIELARTNPS
HHHHHHHHHCCCCEEEEEHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ATAVTLHPGTVDTPLSRPFQRSVPADRLFSPEQSAAHLLAVIDRLTPADSGQLFAWDGSA
CEEEEECCCCCCCCCCCCHHHCCCHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCC
IPF
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 5550 [C]

Specific activity: 66

Km value (mM): 0.26 {methyl-2,3-dehydro-3-hydroxybenzoate}} 0.2 {3-oxocyclohexane-1-carboxylic} 0.3 {2,3-dihydro-2,3-dihydroxybenzoate}} [C]

Substrates: 2,3-Dihydro-2,3-dihydroxybenzoate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 2,3-Dihydro-2,3-dihydroxybenzoate + Nicotinamide adenine dinucleotide <==> 2,3-Dihydroxybenzoate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2152896; 2107980; 2118510; 1372277 [H]