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The map label for this gene is pckA

Identifier: 87200920

GI number: 87200920

Start: 3125015

End: 3126619

Strand: Direct

Name: pckA

Synonym: Saro_2908

Alternate gene names: 87200920

Gene position: 3125015-3126619 (Clockwise)

Preceding gene: 87200911

Following gene: 87200921

Centisome position: 87.74

GC content: 65.67

Gene sequence:

>1605_bases
GTGTCCGATACCACCCTGAACGTGTCCCTCGCCAGCCAGGGCTACCCTGAACCGGCCGAGCTGTTCGCCAACCTCGGCAC
CGCGCCCCTGGTGGAACACGCCGTCCGCAACGGCGAAGGCCTGCTCTGCGTCGATGGCCCGCTGGTGGTCGAGACCGGCA
AGCACACCGGCCGCTCGGCCAAGGACAAGTTCATCGTCCGCGATGCCGAAACCGAAAACACCGTCTGGTGGGGCAAGACC
AACGTCGCGATGTCGCCCGAGCACTTCGCCGCGCTCAAGGCCGACTTCATCAAGGCGCTGGGCGAGAAGGATCGCCTCTA
CGTTGCCGACCTGTTCGGCGGCAGCCAGCCGGAGCACCGCGTCAAGGTGCGCGTGATCAACGAACTCGCGTGGCACAACC
TGTTCATCCGCACCCTGCTGGTGCGCCCGACCACCGACGAGCTGGCCTCGTTCGTGCCGGAATACACGATCATCGACCTG
CCTAGCTTCCGCGCCGACCCGGCTCGCCACGGCAGCCGCAGCGAGACCGTGATCGCGGTGAACTTCACCGAAAAGCTGAT
TCTGATCGGCGGCACGGCCTATGCCGGCGAGATGAAGAAATCGGTCTTCGGCATCCTCAACTACCTGCTGCCGGTAAAGG
GCGTGATGCCGATGCACTGCTCGGCCAACGTCGGCCCCGACGGCAAGACCGCCGTATTCTTCGGCCTTTCCGGCACCGGC
AAGACCACGCTTTCGGCCGATGCTTCGCGCACGCTCATCGGTGACGACGAGCATGGCTGGTCCGACACCGCCGTGTTCAA
CTTCGAAGGCGGCTGCTACGCCAAGATGATCCGCCTCTCGGCCGAAGCCGAACCGGAAATCTTCGCGACGACCAAGCGCT
TCGGCACCGTGCTCGAGAACGTCGTGATCGATCCGGTCACCCGCACGATCGACCTCGACGACAACAGCCTCGCCGAGAAC
AGCCGCGGTTCCTACCCGATCGACTTCATCCCGAACGCGAGCGAGGAAAACCTCGGCCCGGTTCCGGCCAACCTGATCTT
CCTCACCGCCGATGCCTATGGCGTCCTGCCCCCGATCGCGCGCCTCACGCCTGACCAGGCAATGTACCACTTCCTCTCGG
GCTACACCGCCCGCGTCGCCGGTACCGAGATCGGCGTGACCGAACCGGAAGCCACCTTCTCGACCTGCTTCGGCGCGCCG
TTCATGCCGCGTCACCCGTCGGTCTACGGCAACCTCCTCAAGGAGCGCATCGCCAAGGGCGGCGTGAAGTGCTGGCTGGT
CAACACCGGCTGGTCCGGCGGCAAGGCCACGCAGGAAGGCATCAAGCGCATGCCGATCAAGGCCACCCGCGCCCTGCTCA
ACGCCGCTCTCGACGGCAGCCTCAACAGCGCGACCTTCACCAAGGACCCGAACTTCGGCTTTGAAGTTCCGGTCGAGGTT
CCGGGCGTCGACACCAAGCTGCTCGACCCGCGCGGCGCATGGGCCGACGGCGAGGAGTACGACAAGACGGCGCAGGAACT
CGTGCGCAAGTTCGTCGACAACTTCCAGCAGTTCGCCGACCACGTCGACCAGTCGGTCCGCGATGCGGCACCCGTCGCCG
CCTGA

Upstream 100 bases:

>100_bases
CAGGACACTATGCGCGATCCGAGAATCAGCGCATTCGTATTCGCAATCCGTGCGTAGGGCGGCATGTCGCTGCTCGTGCG
CCACCAACGGGAGGCCCCAA

Downstream 100 bases:

>100_bases
TCGCAAGCGACATCGCTGAAACGCTGCGGGGCTCCTTCGGGGGCCCCGTTTCGTTTGCGCGGACACAATCCCGCAATCTT
CGCGAGCTTCGATAACTGTA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MSDTTLNVSLASQGYPEPAELFANLGTAPLVEHAVRNGEGLLCVDGPLVVETGKHTGRSAKDKFIVRDAETENTVWWGKT
NVAMSPEHFAALKADFIKALGEKDRLYVADLFGGSQPEHRVKVRVINELAWHNLFIRTLLVRPTTDELASFVPEYTIIDL
PSFRADPARHGSRSETVIAVNFTEKLILIGGTAYAGEMKKSVFGILNYLLPVKGVMPMHCSANVGPDGKTAVFFGLSGTG
KTTLSADASRTLIGDDEHGWSDTAVFNFEGGCYAKMIRLSAEAEPEIFATTKRFGTVLENVVIDPVTRTIDLDDNSLAEN
SRGSYPIDFIPNASEENLGPVPANLIFLTADAYGVLPPIARLTPDQAMYHFLSGYTARVAGTEIGVTEPEATFSTCFGAP
FMPRHPSVYGNLLKERIAKGGVKCWLVNTGWSGGKATQEGIKRMPIKATRALLNAALDGSLNSATFTKDPNFGFEVPVEV
PGVDTKLLDPRGAWADGEEYDKTAQELVRKFVDNFQQFADHVDQSVRDAAPVAA

Sequences:

>Translated_534_residues
MSDTTLNVSLASQGYPEPAELFANLGTAPLVEHAVRNGEGLLCVDGPLVVETGKHTGRSAKDKFIVRDAETENTVWWGKT
NVAMSPEHFAALKADFIKALGEKDRLYVADLFGGSQPEHRVKVRVINELAWHNLFIRTLLVRPTTDELASFVPEYTIIDL
PSFRADPARHGSRSETVIAVNFTEKLILIGGTAYAGEMKKSVFGILNYLLPVKGVMPMHCSANVGPDGKTAVFFGLSGTG
KTTLSADASRTLIGDDEHGWSDTAVFNFEGGCYAKMIRLSAEAEPEIFATTKRFGTVLENVVIDPVTRTIDLDDNSLAEN
SRGSYPIDFIPNASEENLGPVPANLIFLTADAYGVLPPIARLTPDQAMYHFLSGYTARVAGTEIGVTEPEATFSTCFGAP
FMPRHPSVYGNLLKERIAKGGVKCWLVNTGWSGGKATQEGIKRMPIKATRALLNAALDGSLNSATFTKDPNFGFEVPVEV
PGVDTKLLDPRGAWADGEEYDKTAQELVRKFVDNFQQFADHVDQSVRDAAPVAA
>Mature_533_residues
SDTTLNVSLASQGYPEPAELFANLGTAPLVEHAVRNGEGLLCVDGPLVVETGKHTGRSAKDKFIVRDAETENTVWWGKTN
VAMSPEHFAALKADFIKALGEKDRLYVADLFGGSQPEHRVKVRVINELAWHNLFIRTLLVRPTTDELASFVPEYTIIDLP
SFRADPARHGSRSETVIAVNFTEKLILIGGTAYAGEMKKSVFGILNYLLPVKGVMPMHCSANVGPDGKTAVFFGLSGTGK
TTLSADASRTLIGDDEHGWSDTAVFNFEGGCYAKMIRLSAEAEPEIFATTKRFGTVLENVVIDPVTRTIDLDDNSLAENS
RGSYPIDFIPNASEENLGPVPANLIFLTADAYGVLPPIARLTPDQAMYHFLSGYTARVAGTEIGVTEPEATFSTCFGAPF
MPRHPSVYGNLLKERIAKGGVKCWLVNTGWSGGKATQEGIKRMPIKATRALLNAALDGSLNSATFTKDPNFGFEVPVEVP
GVDTKLLDPRGAWADGEEYDKTAQELVRKFVDNFQQFADHVDQSVRDAAPVAA

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=497, Percent_Identity=49.8993963782696, Blast_Score=474, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6322950, Length=513, Percent_Identity=45.6140350877193, Blast_Score=455, Evalue=1e-129,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_NOVAD (Q2G480)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_498177.1
- ProteinModelPortal:   Q2G480
- SMR:   Q2G480
- STRING:   Q2G480
- GeneID:   3915547
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_2908
- NMPDR:   fig|48935.1.peg.3533
- eggNOG:   COG1866
- HOGENOM:   HBG293387
- OMA:   FAQDLYG
- PhylomeDB:   Q2G480
- ProtClustDB:   PRK09344
- BioCyc:   NARO279238:SARO_2908-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 57889; Mature: 57758

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTTLNVSLASQGYPEPAELFANLGTAPLVEHAVRNGEGLLCVDGPLVVETGKHTGRSA
CCCCEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHCCCEEEEECCCEEEECCCCCCCCC
KDKFIVRDAETENTVWWGKTNVAMSPEHFAALKADFIKALGEKDRLYVADLFGGSQPEHR
CCCEEEEECCCCCCEEECCCCCEECHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCE
VKVRVINELAWHNLFIRTLLVRPTTDELASFVPEYTIIDLPSFRADPARHGSRSETVIAV
EEEEHHHHHHHHHHEEHHHEECCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEEEEE
NFTEKLILIGGTAYAGEMKKSVFGILNYLLPVKGVMPMHCSANVGPDGKTAVFFGLSGTG
EECCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCC
KTTLSADASRTLIGDDEHGWSDTAVFNFEGGCYAKMIRLSAEAEPEIFATTKRFGTVLEN
CEEEECCCCCEEECCCCCCCCCEEEEEECCCCEEEHEECCCCCCCCEEEHHHHHHHHHHH
VVIDPVTRTIDLDDNSLAENSRGSYPIDFIPNASEENLGPVPANLIFLTADAYGVLPPIA
HHCCCCEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCHH
RLTPDQAMYHFLSGYTARVAGTEIGVTEPEATFSTCFGAPFMPRHPSVYGNLLKERIAKG
HCCHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCC
GVKCWLVNTGWSGGKATQEGIKRMPIKATRALLNAALDGSLNSATFTKDPNFGFEVPVEV
CCEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEECCCCCCCEEEEEEC
PGVDTKLLDPRGAWADGEEYDKTAQELVRKFVDNFQQFADHVDQSVRDAAPVAA
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SDTTLNVSLASQGYPEPAELFANLGTAPLVEHAVRNGEGLLCVDGPLVVETGKHTGRSA
CCCEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHCCCEEEEECCCEEEECCCCCCCCC
KDKFIVRDAETENTVWWGKTNVAMSPEHFAALKADFIKALGEKDRLYVADLFGGSQPEHR
CCCEEEEECCCCCCEEECCCCCEECHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCE
VKVRVINELAWHNLFIRTLLVRPTTDELASFVPEYTIIDLPSFRADPARHGSRSETVIAV
EEEEHHHHHHHHHHEEHHHEECCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEEEEE
NFTEKLILIGGTAYAGEMKKSVFGILNYLLPVKGVMPMHCSANVGPDGKTAVFFGLSGTG
EECCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCC
KTTLSADASRTLIGDDEHGWSDTAVFNFEGGCYAKMIRLSAEAEPEIFATTKRFGTVLEN
CEEEECCCCCEEECCCCCCCCCEEEEEECCCCEEEHEECCCCCCCCEEEHHHHHHHHHHH
VVIDPVTRTIDLDDNSLAENSRGSYPIDFIPNASEENLGPVPANLIFLTADAYGVLPPIA
HHCCCCEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCHH
RLTPDQAMYHFLSGYTARVAGTEIGVTEPEATFSTCFGAPFMPRHPSVYGNLLKERIAKG
HCCHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCC
GVKCWLVNTGWSGGKATQEGIKRMPIKATRALLNAALDGSLNSATFTKDPNFGFEVPVEV
CCEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEECCCCCCCEEEEEEC
PGVDTKLLDPRGAWADGEEYDKTAQELVRKFVDNFQQFADHVDQSVRDAAPVAA
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA