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The map label for this gene is ygcF [C]

Identifier: 87200860

GI number: 87200860

Start: 3073305

End: 3074048

Strand: Direct

Name: ygcF [C]

Synonym: Saro_2847

Alternate gene names: 87200860

Gene position: 3073305-3074048 (Clockwise)

Preceding gene: 87200859

Following gene: 87200866

Centisome position: 86.29

GC content: 68.01

Gene sequence:

>744_bases
ATGTTGACCCTGGCCACCGTCAACCCCGGCGAGCCGGAGGTCTTTTCCTCGTTGCAGGGCGAAGGGCCCGGCATGGGGCG
GCCTTCGGTGTTCGTGCGGCTGTCGCGGTGCAACCTTGCGTGCCGCTGGTGCGACACGGCCTATACCTGGCGCTTTGCCG
GCGACAACCGGCCGCACCGCGACGAGGTGGCGTTCGAGAAGGCCGGCAACCAGCTGGTGATGTCGGAAGAAGATACGGCC
GCGCTGATCCTGGTTCATCCGGAGGACCGGCTGGTCATCACCGGGGGCGAGCCACTGTTGCAGGGGGCGGCGCTGGCACG
GCTGGTGGCGCTGCTTAAGGCCGAGCGCCCTGCCCTGCATGTCGAGATCGAGACCAACGGGACCGTGGCGGTACACCCCG
CGCTCGATCCGCTGGTGGACCAGTTCAACGTCAGCCCGAAACTGTCCCATTCGGGCAATCCCGCCGAACTCGCGCTGCTG
CCGGAACGACTGGAAGCCTGGGCGCGGGACCCGCGCGCCTGGTTCAAGTTCGTGGTCGCCGACCCGTCGGACCTTGCCGA
GATTTCGGCGTTGCAGGACCGCTTCGGGATCGCCCCGGATCACCTGTTCGTGATGCCCGAGGGCACTTCAAGCGCGGTGC
TGCGCGACCGGTCGCGCTGGCTGGCAGAGGAAGCGCTGGTGCGCGGCTGGCGGTTCACCGACCGGCTGCATATCCATCTC
TACGGGGATACGCGGGGGACCTGA

Upstream 100 bases:

>100_bases
GGTAGAGAAGGCCCGCGCCGACCACGCCATGCACGCCGCCGAGGCCGAACGGCTCGCAGCGGCGCTGGCACGGCTGGGGT
GACAGGGTGCTGACGCCCCC

Downstream 100 bases:

>100_bases
GGGCTTCGCAAGAGCGGGGGCGAGGCAACTTGCCCTCCCCCAGCCCCTCCCGCAGGCGGGAGGGGAGATTCAGGTTGCCG
GGGGCGATGCCCCGCAAGTC

Product: radical SAM family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MLTLATVNPGEPEVFSSLQGEGPGMGRPSVFVRLSRCNLACRWCDTAYTWRFAGDNRPHRDEVAFEKAGNQLVMSEEDTA
ALILVHPEDRLVITGGEPLLQGAALARLVALLKAERPALHVEIETNGTVAVHPALDPLVDQFNVSPKLSHSGNPAELALL
PERLEAWARDPRAWFKFVVADPSDLAEISALQDRFGIAPDHLFVMPEGTSSAVLRDRSRWLAEEALVRGWRFTDRLHIHL
YGDTRGT

Sequences:

>Translated_247_residues
MLTLATVNPGEPEVFSSLQGEGPGMGRPSVFVRLSRCNLACRWCDTAYTWRFAGDNRPHRDEVAFEKAGNQLVMSEEDTA
ALILVHPEDRLVITGGEPLLQGAALARLVALLKAERPALHVEIETNGTVAVHPALDPLVDQFNVSPKLSHSGNPAELALL
PERLEAWARDPRAWFKFVVADPSDLAEISALQDRFGIAPDHLFVMPEGTSSAVLRDRSRWLAEEALVRGWRFTDRLHIHL
YGDTRGT
>Mature_247_residues
MLTLATVNPGEPEVFSSLQGEGPGMGRPSVFVRLSRCNLACRWCDTAYTWRFAGDNRPHRDEVAFEKAGNQLVMSEEDTA
ALILVHPEDRLVITGGEPLLQGAALARLVALLKAERPALHVEIETNGTVAVHPALDPLVDQFNVSPKLSHSGNPAELALL
PERLEAWARDPRAWFKFVVADPSDLAEISALQDRFGIAPDHLFVMPEGTSSAVLRDRSRWLAEEALVRGWRFTDRLHIHL
YGDTRGT

Specific function: Unknown

COG id: COG0602

COG function: function code O; Organic radical activating enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli ygcF and H.influenzae HI_1189 [H]

Homologues:

Organism=Escherichia coli, GI1789139, Length=238, Percent_Identity=30.2521008403361, Blast_Score=74, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 27303; Mature: 27303

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLATVNPGEPEVFSSLQGEGPGMGRPSVFVRLSRCNLACRWCDTAYTWRFAGDNRPHR
CEEEEECCCCCHHHHHHCCCCCCCCCCCEEEEEEECCCEEEEEECCCEEEEECCCCCCCC
DEVAFEKAGNQLVMSEEDTAALILVHPEDRLVITGGEPLLQGAALARLVALLKAERPALH
HHHHHHHCCCEEEEECCCCEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCEEE
VEIETNGTVAVHPALDPLVDQFNVSPKLSHSGNPAELALLPERLEAWARDPRAWFKFVVA
EEEECCCEEEECCCHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHCCCCHHEEEEEE
DPSDLAEISALQDRFGIAPDHLFVMPEGTSSAVLRDRSRWLAEEALVRGWRFTDRLHIHL
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEE
YGDTRGT
ECCCCCC
>Mature Secondary Structure
MLTLATVNPGEPEVFSSLQGEGPGMGRPSVFVRLSRCNLACRWCDTAYTWRFAGDNRPHR
CEEEEECCCCCHHHHHHCCCCCCCCCCCEEEEEEECCCEEEEEECCCEEEEECCCCCCCC
DEVAFEKAGNQLVMSEEDTAALILVHPEDRLVITGGEPLLQGAALARLVALLKAERPALH
HHHHHHHCCCEEEEECCCCEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCEEE
VEIETNGTVAVHPALDPLVDQFNVSPKLSHSGNPAELALLPERLEAWARDPRAWFKFVVA
EEEECCCEEEECCCHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHCCCCHHEEEEEE
DPSDLAEISALQDRFGIAPDHLFVMPEGTSSAVLRDRSRWLAEEALVRGWRFTDRLHIHL
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEE
YGDTRGT
ECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]