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The map label for this gene is lcfB [H]

Identifier: 87200352

GI number: 87200352

Start: 2485636

End: 2487165

Strand: Reverse

Name: lcfB [H]

Synonym: Saro_2338

Alternate gene names: 87200352

Gene position: 2487165-2485636 (Counterclockwise)

Preceding gene: 87200357

Following gene: 87200351

Centisome position: 69.83

GC content: 68.5

Gene sequence:

>1530_bases
GTGACCGCCGAGCCCGATCCCACCGTCCATCCGCTCGATCATCTTGCCCTTCGCGGAGAGCGGGGGGCGCCTGCGCTCGT
GCTCAGGAACCACACCCTAACCCACGAAGCGTTAAATGCTCGTGTAGGACTGCTCGCGAACTGGCTGCAATCGCGCGTGC
CGGAGCGCGGCGCGCGGGTGGCGACGTGGCTGCCGAAGTCGGAGCTGTCCTGCCTCATGCCGCTGGCGGCGGTCCGTGCT
AGTCTTGTGCACGTGCCGGTCAATCCGCTGCTCAAGCGCGGGCAGGTCGCGCATATCCTGGCCGACAGCGGCGCGGCGCT
GCTCGTGTCGAACAAGGCGCGGCTGGATTCGCTGGAACCCGGCGACGCGTCGTGCCCACTGATCGAGGAGCCCGCCGCAT
GGGCCGAGGCCGAAGCGCTTGGCGGGCAATTGCCGCCATCGGACGCCGCGCCGGACAGCCTTGCCGCGATCCTCTACACC
AGCGGGTCGACCGGAAGGCCCAAGGGCGTGATGCTGAGCCAGGCGAACCTCTGGCTGGGGGCGGTCAGCGTGGCGCACTA
TCTGCGGCTGTCGCCCGCAGACCGGGTCCTTGCCGTCCTGCCGCTGGCGTTCGACTATGGCCAGAACCAGTTGCTCTCGA
CCTGGTATGCGGGTGGCAGCGTGGTCCCGCTCGATTATCTGACGCCGCGCGACGTCGTGAAAGCCGTCGAGCGGCATGGG
ATCACGACGATTGCGGCAGTTCCGCCGCTGTGGCTGCAACTTGCCGAACTGGACTGGCCTGAAGCTGCCCGCTCGCTGCG
GCGCCTCACCAACAGCGGCGGCGCGCTGACGCCGTCGCTGGTTCGCGCGCTGCGCACGCGCTTCCCCGAGGCGGACCTCT
ACCCGATGTACGGCCTGACCGAGGCGTTCCGCTCAACGTATCTGGACCCCGCGCTCGTTGACAGCCACCCGACATCGATC
GGCAGGGCCATTCCCTTTGCAGAAGTTAGTGTCGTCAATGACTTGGGGGATGAAGCTGAGGTCGAGGAAGAGGGTGAGCT
AGTTCACGCCGGCCCTTTGGTGGCGCAAGGTTACTGGCAGGATGCGGAGCGTACCGCCGAGCGGTTCAGGCCTGCGCCCC
CGTTCTCGAAGCTTGGCGGGATGGCGGTCTGGTCGGGGGATCGGGTCCGGCGCGATGCGGAAGGCCTGCTGCATTTCGTC
GGGCGGCGCGACGCCATGATCAAGACCAGCGGCAACCGCGTGAGCCCGCAAGAGGTCGAGGAAGCCGCGGTGGCGACGGG
CCTCGTCGCGGAGGCCGTGGCGCTGGGCCTGCCGGATCCGCACCTGGGCCATGCGATCCATCTCGTCGCTCGCGCTTCTG
GCGACGTGGAGGCGGCACGGGCCGGACTGCTTCCGGCACTGACGCGCGCGTTGCCCAACTTCATGGTGCCGCGCCAGGTG
CATTGGCGCCAGGTCATGCCGGTCAGCCCCAATGGCAAGCTCGACCGCGTTGCGCTGGCCGCCGAACTGGCGCAGGACGT
GCAGGCATGA

Upstream 100 bases:

>100_bases
GAGGCGCCTTGCAACCCCTTCAGGCGCGGGTCGGCTTGCGCTTCCTCGAGACTGGCGTGATAGACGTTCAACGTTCCAGC
ATTCCTTAGTCGAGACCTGC

Downstream 100 bases:

>100_bases
AACCGCTCGGCCCGATCCCGCCCGGATACGAAACCATCGACGGCGAACTGGCCGTGGATGGACACAAGGTTACCGATCTC
GTGATGCAGGCCGGCGGAAC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MTAEPDPTVHPLDHLALRGERGAPALVLRNHTLTHEALNARVGLLANWLQSRVPERGARVATWLPKSELSCLMPLAAVRA
SLVHVPVNPLLKRGQVAHILADSGAALLVSNKARLDSLEPGDASCPLIEEPAAWAEAEALGGQLPPSDAAPDSLAAILYT
SGSTGRPKGVMLSQANLWLGAVSVAHYLRLSPADRVLAVLPLAFDYGQNQLLSTWYAGGSVVPLDYLTPRDVVKAVERHG
ITTIAAVPPLWLQLAELDWPEAARSLRRLTNSGGALTPSLVRALRTRFPEADLYPMYGLTEAFRSTYLDPALVDSHPTSI
GRAIPFAEVSVVNDLGDEAEVEEEGELVHAGPLVAQGYWQDAERTAERFRPAPPFSKLGGMAVWSGDRVRRDAEGLLHFV
GRRDAMIKTSGNRVSPQEVEEAAVATGLVAEAVALGLPDPHLGHAIHLVARASGDVEAARAGLLPALTRALPNFMVPRQV
HWRQVMPVSPNGKLDRVALAAELAQDVQA

Sequences:

>Translated_509_residues
MTAEPDPTVHPLDHLALRGERGAPALVLRNHTLTHEALNARVGLLANWLQSRVPERGARVATWLPKSELSCLMPLAAVRA
SLVHVPVNPLLKRGQVAHILADSGAALLVSNKARLDSLEPGDASCPLIEEPAAWAEAEALGGQLPPSDAAPDSLAAILYT
SGSTGRPKGVMLSQANLWLGAVSVAHYLRLSPADRVLAVLPLAFDYGQNQLLSTWYAGGSVVPLDYLTPRDVVKAVERHG
ITTIAAVPPLWLQLAELDWPEAARSLRRLTNSGGALTPSLVRALRTRFPEADLYPMYGLTEAFRSTYLDPALVDSHPTSI
GRAIPFAEVSVVNDLGDEAEVEEEGELVHAGPLVAQGYWQDAERTAERFRPAPPFSKLGGMAVWSGDRVRRDAEGLLHFV
GRRDAMIKTSGNRVSPQEVEEAAVATGLVAEAVALGLPDPHLGHAIHLVARASGDVEAARAGLLPALTRALPNFMVPRQV
HWRQVMPVSPNGKLDRVALAAELAQDVQA
>Mature_508_residues
TAEPDPTVHPLDHLALRGERGAPALVLRNHTLTHEALNARVGLLANWLQSRVPERGARVATWLPKSELSCLMPLAAVRAS
LVHVPVNPLLKRGQVAHILADSGAALLVSNKARLDSLEPGDASCPLIEEPAAWAEAEALGGQLPPSDAAPDSLAAILYTS
GSTGRPKGVMLSQANLWLGAVSVAHYLRLSPADRVLAVLPLAFDYGQNQLLSTWYAGGSVVPLDYLTPRDVVKAVERHGI
TTIAAVPPLWLQLAELDWPEAARSLRRLTNSGGALTPSLVRALRTRFPEADLYPMYGLTEAFRSTYLDPALVDSHPTSIG
RAIPFAEVSVVNDLGDEAEVEEEGELVHAGPLVAQGYWQDAERTAERFRPAPPFSKLGGMAVWSGDRVRRDAEGLLHFVG
RRDAMIKTSGNRVSPQEVEEAAVATGLVAEAVALGLPDPHLGHAIHLVARASGDVEAARAGLLPALTRALPNFMVPRQVH
WRQVMPVSPNGKLDRVALAAELAQDVQA

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=535, Percent_Identity=23.5514018691589, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI42544132, Length=474, Percent_Identity=23.8396624472574, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI38505220, Length=473, Percent_Identity=26.0042283298097, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI157311624, Length=471, Percent_Identity=23.9915074309979, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI157311622, Length=471, Percent_Identity=23.9915074309979, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI187761345, Length=455, Percent_Identity=26.5934065934066, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI187761343, Length=455, Percent_Identity=26.5934065934066, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI58082049, Length=471, Percent_Identity=23.5668789808917, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI122937307, Length=472, Percent_Identity=23.5169491525424, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI195947378, Length=383, Percent_Identity=25.065274151436, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI115511026, Length=481, Percent_Identity=22.8690228690229, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1788107, Length=528, Percent_Identity=28.030303030303, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI1786801, Length=502, Percent_Identity=27.0916334661355, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI145693145, Length=521, Percent_Identity=25.1439539347409, Blast_Score=106, Evalue=5e-24,
Organism=Escherichia coli, GI221142682, Length=491, Percent_Identity=24.6435845213849, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790505, Length=401, Percent_Identity=25.6857855361596, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789201, Length=388, Percent_Identity=26.8041237113402, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786810, Length=367, Percent_Identity=24.7956403269755, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI32563687, Length=454, Percent_Identity=23.7885462555066, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17558820, Length=478, Percent_Identity=23.8493723849372, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17557194, Length=435, Percent_Identity=23.2183908045977, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17538037, Length=438, Percent_Identity=23.7442922374429, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17531443, Length=358, Percent_Identity=25.4189944134078, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17560308, Length=523, Percent_Identity=23.1357552581262, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI71994703, Length=519, Percent_Identity=21.3872832369942, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71994694, Length=519, Percent_Identity=21.3872832369942, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71994690, Length=519, Percent_Identity=21.3872832369942, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17559526, Length=309, Percent_Identity=25.5663430420712, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71996755, Length=448, Percent_Identity=23.2142857142857, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71985884, Length=327, Percent_Identity=23.2415902140673, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=364, Percent_Identity=26.0989010989011, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6323182, Length=476, Percent_Identity=22.6890756302521, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24581924, Length=364, Percent_Identity=26.3736263736264, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI18859661, Length=526, Percent_Identity=23.9543726235741, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24648260, Length=290, Percent_Identity=25.5172413793103, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21355181, Length=307, Percent_Identity=25.4071661237785, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21356947, Length=463, Percent_Identity=22.8941684665227, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 54502; Mature: 54371

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAEPDPTVHPLDHLALRGERGAPALVLRNHTLTHEALNARVGLLANWLQSRVPERGARV
CCCCCCCCCCCHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEE
ATWLPKSELSCLMPLAAVRASLVHVPVNPLLKRGQVAHILADSGAALLVSNKARLDSLEP
EEECCHHHHHHHHHHHHHHHHHEECCCHHHHHCCCEEEEEECCCCEEEEECCHHCCCCCC
GDASCPLIEEPAAWAEAEALGGQLPPSDAAPDSLAAILYTSGSTGRPKGVMLSQANLWLG
CCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEECCCHHHH
AVSVAHYLRLSPADRVLAVLPLAFDYGQNQLLSTWYAGGSVVPLDYLTPRDVVKAVERHG
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEECCCCHHHHHHHHHHCC
ITTIAAVPPLWLQLAELDWPEAARSLRRLTNSGGALTPSLVRALRTRFPEADLYPMYGLT
CEEEECCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHH
EAFRSTYLDPALVDSHPTSIGRAIPFAEVSVVNDLGDEAEVEEEGELVHAGPLVAQGYWQ
HHHHHHCCCCHHCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCCEEECCCHHHCCCHH
DAERTAERFRPAPPFSKLGGMAVWSGDRVRRDAEGLLHFVGRRDAMIKTSGNRVSPQEVE
HHHHHHHHCCCCCCHHHHCCEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
EAAVATGLVAEAVALGLPDPHLGHAIHLVARASGDVEAARAGLLPALTRALPNFMVPRQV
HHHHHHHHHHHHHHHCCCCCCCCHHEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
HWRQVMPVSPNGKLDRVALAAELAQDVQA
CHHEEECCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAEPDPTVHPLDHLALRGERGAPALVLRNHTLTHEALNARVGLLANWLQSRVPERGARV
CCCCCCCCCCHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEE
ATWLPKSELSCLMPLAAVRASLVHVPVNPLLKRGQVAHILADSGAALLVSNKARLDSLEP
EEECCHHHHHHHHHHHHHHHHHEECCCHHHHHCCCEEEEEECCCCEEEEECCHHCCCCCC
GDASCPLIEEPAAWAEAEALGGQLPPSDAAPDSLAAILYTSGSTGRPKGVMLSQANLWLG
CCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEECCCHHHH
AVSVAHYLRLSPADRVLAVLPLAFDYGQNQLLSTWYAGGSVVPLDYLTPRDVVKAVERHG
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEECCCCHHHHHHHHHHCC
ITTIAAVPPLWLQLAELDWPEAARSLRRLTNSGGALTPSLVRALRTRFPEADLYPMYGLT
CEEEECCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHH
EAFRSTYLDPALVDSHPTSIGRAIPFAEVSVVNDLGDEAEVEEEGELVHAGPLVAQGYWQ
HHHHHHCCCCHHCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCCEEECCCHHHCCCHH
DAERTAERFRPAPPFSKLGGMAVWSGDRVRRDAEGLLHFVGRRDAMIKTSGNRVSPQEVE
HHHHHHHHCCCCCCHHHHCCEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
EAAVATGLVAEAVALGLPDPHLGHAIHLVARASGDVEAARAGLLPALTRALPNFMVPRQV
HHHHHHHHHHHHHHHCCCCCCCCHHEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
HWRQVMPVSPNGKLDRVALAAELAQDVQA
CHHEEECCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]