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The map label for this gene is thiE [H]

Identifier: 87199979

GI number: 87199979

Start: 2080800

End: 2081441

Strand: Reverse

Name: thiE [H]

Synonym: Saro_1962

Alternate gene names: 87199979

Gene position: 2081441-2080800 (Counterclockwise)

Preceding gene: 87199981

Following gene: 87199978

Centisome position: 58.44

GC content: 61.53

Gene sequence:

>642_bases
ATGGCCAAGCTTTCCTCCGAACTCTACCTGATCTCGCCTCTCGACGTCGAAGGCACCTTTCCAGAGCGACTGGAGCGGGC
GCTCGACGCAGGGCCCGTAGCCGCATTCCAGTTTCGCGTGAAGGATGTGGACGAGCATTCCGCTGCGCGCATGGCCGAAC
CGCTCCAGCGCATCTGTGCCGATCGTGATGTGGCGTTCATCGTCAACGATTCCATAAGCTTGGCAAAGCGCCTGGGCGCC
GACGGGGTCCACCTTGGCCAGAAGGATGGTGACGTGAAGGATGCGCGCGAACGTCTTGGGCGCAACGCCCAGATAGGTGT
CACCTGTCATGACAGCCGGCACCTTGCGATGGATGCGGGTGAGGCGGGGGCCGATTACGTTGCCTTCGGCGCATTCTTTC
CGTCGAGCACCAAGGAAACCAAGCACGTCGCGGGTCTCGATCTGCTCGAATGGTGGCAATCGATCTTCGAACTTCCATGC
GTTGCAATTGGCGGCATCACCCCGGCAAATTGTGCCCCGTTGATCCGGGCCGGCGCGGACTTTCTGGCGGTCAGCAATGC
CGTGTGGGACGGGGACGAGGCAGCCAATGTAGCGGCCTTCTTCGACGCAATCCGCGCAAACCCGCGCATCACCGAGCAAT
AA

Upstream 100 bases:

>100_bases
GGCGAGGAATGCGTTTTTCATCCAGTCACAATGCGGTACGCGCCTGAACCGTTCCGGAACCGTCATTGCAGCATGGCGAT
CGAGGCGATAAGGCGCCCGC

Downstream 100 bases:

>100_bases
GTCGAGGAACGTCGGGCAGCGCAAAGGATTGATCCGGAGCACAAGTTCCGGAGTTTCGATGTTACGCCACACAGCTGCCG
CGCTCGCCATCCTAGCCCTT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MAKLSSELYLISPLDVEGTFPERLERALDAGPVAAFQFRVKDVDEHSAARMAEPLQRICADRDVAFIVNDSISLAKRLGA
DGVHLGQKDGDVKDARERLGRNAQIGVTCHDSRHLAMDAGEAGADYVAFGAFFPSSTKETKHVAGLDLLEWWQSIFELPC
VAIGGITPANCAPLIRAGADFLAVSNAVWDGDEAANVAAFFDAIRANPRITEQ

Sequences:

>Translated_213_residues
MAKLSSELYLISPLDVEGTFPERLERALDAGPVAAFQFRVKDVDEHSAARMAEPLQRICADRDVAFIVNDSISLAKRLGA
DGVHLGQKDGDVKDARERLGRNAQIGVTCHDSRHLAMDAGEAGADYVAFGAFFPSSTKETKHVAGLDLLEWWQSIFELPC
VAIGGITPANCAPLIRAGADFLAVSNAVWDGDEAANVAAFFDAIRANPRITEQ
>Mature_212_residues
AKLSSELYLISPLDVEGTFPERLERALDAGPVAAFQFRVKDVDEHSAARMAEPLQRICADRDVAFIVNDSISLAKRLGAD
GVHLGQKDGDVKDARERLGRNAQIGVTCHDSRHLAMDAGEAGADYVAFGAFFPSSTKETKHVAGLDLLEWWQSIFELPCV
AIGGITPANCAPLIRAGADFLAVSNAVWDGDEAANVAAFFDAIRANPRITEQ

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=179, Percent_Identity=32.4022346368715, Blast_Score=67, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6325042, Length=177, Percent_Identity=31.638418079096, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 22953; Mature: 22822

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLSSELYLISPLDVEGTFPERLERALDAGPVAAFQFRVKDVDEHSAARMAEPLQRICA
CCCCCCCEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHC
DRDVAFIVNDSISLAKRLGADGVHLGQKDGDVKDARERLGRNAQIGVTCHDSRHLAMDAG
CCCEEEEECCCHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCEEEEECC
EAGADYVAFGAFFPSSTKETKHVAGLDLLEWWQSIFELPCVAIGGITPANCAPLIRAGAD
CCCCCEEEEEEECCCCCCCHHHHHCCHHHHHHHHHHHCCHHEECCCCCCCCHHHHHCCCH
FLAVSNAVWDGDEAANVAAFFDAIRANPRITEQ
HHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AKLSSELYLISPLDVEGTFPERLERALDAGPVAAFQFRVKDVDEHSAARMAEPLQRICA
CCCCCCEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHC
DRDVAFIVNDSISLAKRLGADGVHLGQKDGDVKDARERLGRNAQIGVTCHDSRHLAMDAG
CCCEEEEECCCHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCEEEEECC
EAGADYVAFGAFFPSSTKETKHVAGLDLLEWWQSIFELPCVAIGGITPANCAPLIRAGAD
CCCCCEEEEEEECCCCCCCHHHHHCCHHHHHHHHHHHCCHHEECCCCCCCCHHHHHCCCH
FLAVSNAVWDGDEAANVAAFFDAIRANPRITEQ
HHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA