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The map label for this gene is slt [H]

Identifier: 87199796

GI number: 87199796

Start: 1875794

End: 1877572

Strand: Reverse

Name: slt [H]

Synonym: Saro_1779

Alternate gene names: 87199796

Gene position: 1877572-1875794 (Counterclockwise)

Preceding gene: 87199797

Following gene: 87199795

Centisome position: 52.72

GC content: 69.31

Gene sequence:

>1779_bases
GTGAAACTGGCTCGCGCCGCTCATTGCTCGCTCGCAGCGCTGGCGACCTGCTTCGCCGTGCTGGGCGCCGTTCCGGCCAA
TGCCAACAGCGCCGCCGTCGACTATTTCCGTGGCCGGGCGGACCGTACCGCCGTGCCCTCGCTGCTCAGCCAGGACGACC
GCTCGTTCTACAAGTCACTGTTCGGCGCGATCGAGCGAGAAGAATGGACCCAGGTCCAGGCCCTGTTCGCACAGCGTGCC
GACGGCCCGCTGCATGACGTTGCCCGCAGCCAGTACATGCTCGCCGCAACCTCGCCCAAGGCCGAACTCGGCGCGCTGAC
CGACGTGCTGAACCGCGCGCCGAACCTGCCTTGGGCCGAACAGCTTGGCCGCCTGGCGCTCAAGCGCGGGGCGAGCGAGC
TTCCCGCCCTACCGCAGGGTCAGCGCCTGGTTTCGCTGCCCAGCGTATCGAAGCGTATCCGCCCGCGCTCCATCACCGAC
GGCTCCATGCCCGATGCAGTCTCTGCCGCGATCATGGACCGCATCAAGTTCGACGATCCGGTCGGAGCGCGCGTGCTGCT
CGACGGCGTCGACGCCACCCTTTCGCCCGAAGCGCGGACCGAATGGCGCCAGAAGATCGGCTGGGCCTTCTACATCGAGA
ACGACGACGCGACCGCCCGCGCCATTGCGCTTTCGGCTGCGGAAGGCGGCGCGGGGCCTTGGGTATCCGAGGCGCATTGG
ACCGCAGGCCTTGCGAGCTGGCGCCTTGGCGATTGCATGGCCGCTGCGGAATCCTTCGAAAGGACTGCCGCCCTCGCCCA
CAACGAGGAACTCGCCTCGGCCGCGAACTACTGGGCAAGCCGCGCCTGGCTGCGCTGCCGCAAGCCCGAGAAGGTTTCCG
GCGCCCTGCGCTCTGCCGCGCGATACCGCGACACGCTCTACGGCATGATGGCCAGCGAGGCGCTCGGCCTGCGCGATAGC
CAGCCCGCCGCCGCCCCCGATTTCTCGCAGGCGGACTGGCAGCAATTACGCGACGTTCCCAACATCCGCACCGCCGTCCA
GCTTGCCGAGATCGGCGAGGACGGGCTCGCGGACGAGATCCTGCGTCATCAGGCGCGCATCGGTGCGCCGGGCCAGTACG
CCCCGCTCTCGCGGCTTGCCCGCGACCTCGGCCTGCCCTCGACCCAGCTCTGGATGGCGTACAACGCGCCTTCGGGTGCC
CGGCCCGACGAAGCCGCCCGCTTCCCCGCGCCCAAGTGGACCCCGGCAACCGGCTGGAAGGTCGACCCGGCGCTCCTCTT
CGCCCATTCGTTGCAGGAATCGAACTTCCGCACCGCCGTTACCAGCCCGGCCGGCGCCAAGGGCCTCATGCAGGTGCGCC
CCGGCACCGCGCGCGACATGGCCCGCGTGGAACCGCTCATGGCCGGGCGCGATCGCCAGCTCGACCTGCCGGACGTGAAC
CTCGCGTTCGGCCAGCAGTATCTCCAGCAGCTCCGCGATGCCGAGGCCACGCAAGGCCTGCTGCCCAAGGTCATGGCGGC
CTACAACGCCGGGCCGCTGCCGGTGGCGCGCTGGAACACCGAGATCCGTGGCGGCAGCGATCCGCTGCTGTGGATGGAAT
CGATCCCCTACTGGGAAACGCGCGGCTATGTGGCCATCGTCATGCGCAACTACTGGATGTACGAAAGGCAGGCGGGCGGC
CCGTCCGACAGCCGGCTCGGCCTCGTCCAGGGCATGTGGCCGAAGTTCCCGGGCCTTGCGGGAGCGGACAACGTAAGGAT
CGCTTCGCGTGGCAATTGA

Upstream 100 bases:

>100_bases
CCGCATTGCCCGCGTGGGATCTTGAGGCAATGCTGGCACGGCCCGCGCTCAAGGCGGGCTTCTGGAGCCGCTGGCTCGAC
TGGACGGGGACTGGAACGTA

Downstream 100 bases:

>100_bases
TCCCTCCCGCGAATTCAAGCCTATCAACATCGCCCTGCTGACCGTCTCCGACACCCGTGGCCCTGACGAGGACACCTCTG
GCGACATCCTCGCTGAACGC

Product: lytic transglycosylase catalytic subunit

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MKLARAAHCSLAALATCFAVLGAVPANANSAAVDYFRGRADRTAVPSLLSQDDRSFYKSLFGAIEREEWTQVQALFAQRA
DGPLHDVARSQYMLAATSPKAELGALTDVLNRAPNLPWAEQLGRLALKRGASELPALPQGQRLVSLPSVSKRIRPRSITD
GSMPDAVSAAIMDRIKFDDPVGARVLLDGVDATLSPEARTEWRQKIGWAFYIENDDATARAIALSAAEGGAGPWVSEAHW
TAGLASWRLGDCMAAAESFERTAALAHNEELASAANYWASRAWLRCRKPEKVSGALRSAARYRDTLYGMMASEALGLRDS
QPAAAPDFSQADWQQLRDVPNIRTAVQLAEIGEDGLADEILRHQARIGAPGQYAPLSRLARDLGLPSTQLWMAYNAPSGA
RPDEAARFPAPKWTPATGWKVDPALLFAHSLQESNFRTAVTSPAGAKGLMQVRPGTARDMARVEPLMAGRDRQLDLPDVN
LAFGQQYLQQLRDAEATQGLLPKVMAAYNAGPLPVARWNTEIRGGSDPLLWMESIPYWETRGYVAIVMRNYWMYERQAGG
PSDSRLGLVQGMWPKFPGLAGADNVRIASRGN

Sequences:

>Translated_592_residues
MKLARAAHCSLAALATCFAVLGAVPANANSAAVDYFRGRADRTAVPSLLSQDDRSFYKSLFGAIEREEWTQVQALFAQRA
DGPLHDVARSQYMLAATSPKAELGALTDVLNRAPNLPWAEQLGRLALKRGASELPALPQGQRLVSLPSVSKRIRPRSITD
GSMPDAVSAAIMDRIKFDDPVGARVLLDGVDATLSPEARTEWRQKIGWAFYIENDDATARAIALSAAEGGAGPWVSEAHW
TAGLASWRLGDCMAAAESFERTAALAHNEELASAANYWASRAWLRCRKPEKVSGALRSAARYRDTLYGMMASEALGLRDS
QPAAAPDFSQADWQQLRDVPNIRTAVQLAEIGEDGLADEILRHQARIGAPGQYAPLSRLARDLGLPSTQLWMAYNAPSGA
RPDEAARFPAPKWTPATGWKVDPALLFAHSLQESNFRTAVTSPAGAKGLMQVRPGTARDMARVEPLMAGRDRQLDLPDVN
LAFGQQYLQQLRDAEATQGLLPKVMAAYNAGPLPVARWNTEIRGGSDPLLWMESIPYWETRGYVAIVMRNYWMYERQAGG
PSDSRLGLVQGMWPKFPGLAGADNVRIASRGN
>Mature_592_residues
MKLARAAHCSLAALATCFAVLGAVPANANSAAVDYFRGRADRTAVPSLLSQDDRSFYKSLFGAIEREEWTQVQALFAQRA
DGPLHDVARSQYMLAATSPKAELGALTDVLNRAPNLPWAEQLGRLALKRGASELPALPQGQRLVSLPSVSKRIRPRSITD
GSMPDAVSAAIMDRIKFDDPVGARVLLDGVDATLSPEARTEWRQKIGWAFYIENDDATARAIALSAAEGGAGPWVSEAHW
TAGLASWRLGDCMAAAESFERTAALAHNEELASAANYWASRAWLRCRKPEKVSGALRSAARYRDTLYGMMASEALGLRDS
QPAAAPDFSQADWQQLRDVPNIRTAVQLAEIGEDGLADEILRHQARIGAPGQYAPLSRLARDLGLPSTQLWMAYNAPSGA
RPDEAARFPAPKWTPATGWKVDPALLFAHSLQESNFRTAVTSPAGAKGLMQVRPGTARDMARVEPLMAGRDRQLDLPDVN
LAFGQQYLQQLRDAEATQGLLPKVMAAYNAGPLPVARWNTEIRGGSDPLLWMESIPYWETRGYVAIVMRNYWMYERQAGG
PSDSRLGLVQGMWPKFPGLAGADNVRIASRGN

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=135, Percent_Identity=36.2962962962963, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 64415; Mature: 64415

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLARAAHCSLAALATCFAVLGAVPANANSAAVDYFRGRADRTAVPSLLSQDDRSFYKSL
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
FGAIEREEWTQVQALFAQRADGPLHDVARSQYMLAATSPKAELGALTDVLNRAPNLPWAE
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCEEEEECCCCHHHHHHHHHHHHCCCCCHHH
QLGRLALKRGASELPALPQGQRLVSLPSVSKRIRPRSITDGSMPDAVSAAIMDRIKFDDP
HHHHHHHHCCHHHCCCCCCCCEEEECCCHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCC
VGARVLLDGVDATLSPEARTEWRQKIGWAFYIENDDATARAIALSAAEGGAGPWVSEAHW
CCCEEEEECCCCCCCCHHHHHHHHHCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCCHH
TAGLASWRLGDCMAAAESFERTAALAHNEELASAANYWASRAWLRCRKPEKVSGALRSAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
RYRDTLYGMMASEALGLRDSQPAAAPDFSQADWQQLRDVPNIRTAVQLAEIGEDGLADEI
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHH
LRHQARIGAPGQYAPLSRLARDLGLPSTQLWMAYNAPSGARPDEAARFPAPKWTPATGWK
HHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHCCCCCCCCCCCCCCC
VDPALLFAHSLQESNFRTAVTSPAGAKGLMQVRPGTARDMARVEPLMAGRDRQLDLPDVN
CCHHHHHHHHHHCCCCCEEECCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCCH
LAFGQQYLQQLRDAEATQGLLPKVMAAYNAGPLPVARWNTEIRGGSDPLLWMESIPYWET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEEHHCCCCCCC
RGYVAIVMRNYWMYERQAGGPSDSRLGLVQGMWPKFPGLAGADNVRIASRGN
CCEEEEEEHHHHHHHHCCCCCCCCCEEHHHCCCCCCCCCCCCCCEEEECCCC
>Mature Secondary Structure
MKLARAAHCSLAALATCFAVLGAVPANANSAAVDYFRGRADRTAVPSLLSQDDRSFYKSL
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
FGAIEREEWTQVQALFAQRADGPLHDVARSQYMLAATSPKAELGALTDVLNRAPNLPWAE
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCEEEEECCCCHHHHHHHHHHHHCCCCCHHH
QLGRLALKRGASELPALPQGQRLVSLPSVSKRIRPRSITDGSMPDAVSAAIMDRIKFDDP
HHHHHHHHCCHHHCCCCCCCCEEEECCCHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCC
VGARVLLDGVDATLSPEARTEWRQKIGWAFYIENDDATARAIALSAAEGGAGPWVSEAHW
CCCEEEEECCCCCCCCHHHHHHHHHCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCCHH
TAGLASWRLGDCMAAAESFERTAALAHNEELASAANYWASRAWLRCRKPEKVSGALRSAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
RYRDTLYGMMASEALGLRDSQPAAAPDFSQADWQQLRDVPNIRTAVQLAEIGEDGLADEI
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHH
LRHQARIGAPGQYAPLSRLARDLGLPSTQLWMAYNAPSGARPDEAARFPAPKWTPATGWK
HHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHCCCCCCCCCCCCCCC
VDPALLFAHSLQESNFRTAVTSPAGAKGLMQVRPGTARDMARVEPLMAGRDRQLDLPDVN
CCHHHHHHHHHHCCCCCEEECCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCCH
LAFGQQYLQQLRDAEATQGLLPKVMAAYNAGPLPVARWNTEIRGGSDPLLWMESIPYWET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEEHHCCCCCCC
RGYVAIVMRNYWMYERQAGGPSDSRLGLVQGMWPKFPGLAGADNVRIASRGN
CCEEEEEEHHHHHHHHCCCCCCCCCEEHHHCCCCCCCCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]