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The map label for this gene is rppH

Identifier: 87199772

GI number: 87199772

Start: 1849895

End: 1850380

Strand: Reverse

Name: rppH

Synonym: Saro_1755

Alternate gene names: 87199772

Gene position: 1850380-1849895 (Counterclockwise)

Preceding gene: 87199773

Following gene: 87199766

Centisome position: 51.95

GC content: 63.99

Gene sequence:

>486_bases
ATGAACGACGCGTTTGCCGGGCTTCCCTATCGTCCCTGTGTCGGGGTCATGCTGGTCAATTCGCAGGGCCGCGTGTTCGT
CGGTCGGCGCATCGACGACAAGGATGGGGTTGCCTGGCAGATGCCGCAGGGCGGGATCGACGATGGCGAGGAACTTCATC
CCGCCGCGCTGCGCGAACTTTCCGAGGAAACCGGCGTCGCGGCGGAACTCGTCACGATCATCGCGGAAAGCCGCGAGGAA
CACCTCTACGACCTGCCGGACGAACTGATCGGCAAGCTGTGGGGCGGCCAGTATCGCGGGCAGCGCCAGAAGTGGCTGCT
GCTCCGCTTTGCCGGAGAAGATACCGACATCCGCCTCGATGCACACGATCCGGCCGAGTTCAGCGAGTGGCGCTGGGTCG
AACCTGAACAGCTTCCCGACCTGATCGTGCCGTTCAAGCGCCGGGTCTACCGCCAGGTCGTCGACGAATTTCGCGACCTG
ATCTGA

Upstream 100 bases:

>100_bases
CGATCCACGGCTTCACGCAGGTGCGCAAGGCGATCCCCAGCGCACAGGCCGATATGCAGTCGATTTTCGCAGCCATCAGG
CTGCTGCTGGAAAGGTTGAA

Downstream 100 bases:

>100_bases
ACGGCGTCGCTAGTTCGCTTCAACCTTCGGCTCGGCGGTAACCGCCTCTGCGGACATGACCTTGGGCGTCGGGCCGCGAG
CGATGGCGGCGGAAAGTTCG

Product: NUDIX hydrolase

Products: NA

Alternate protein names: (Di)nucleoside polyphosphate hydrolase

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MNDAFAGLPYRPCVGVMLVNSQGRVFVGRRIDDKDGVAWQMPQGGIDDGEELHPAALRELSEETGVAAELVTIIAESREE
HLYDLPDELIGKLWGGQYRGQRQKWLLLRFAGEDTDIRLDAHDPAEFSEWRWVEPEQLPDLIVPFKRRVYRQVVDEFRDL
I

Sequences:

>Translated_161_residues
MNDAFAGLPYRPCVGVMLVNSQGRVFVGRRIDDKDGVAWQMPQGGIDDGEELHPAALRELSEETGVAAELVTIIAESREE
HLYDLPDELIGKLWGGQYRGQRQKWLLLRFAGEDTDIRLDAHDPAEFSEWRWVEPEQLPDLIVPFKRRVYRQVVDEFRDL
I
>Mature_161_residues
MNDAFAGLPYRPCVGVMLVNSQGRVFVGRRIDDKDGVAWQMPQGGIDDGEELHPAALRELSEETGVAAELVTIIAESREE
HLYDLPDELIGKLWGGQYRGQRQKWLLLRFAGEDTDIRLDAHDPAEFSEWRWVEPEQLPDLIVPFKRRVYRQVVDEFRDL
I

Specific function: Accelerates the degradation of transcripts by removing pyrophosphate from the 5'-end of triphosphorylated RNA, leading to a more labile monophosphorylated state that can stimulate subsequent ribonuclease cleavage

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain

Homologues:

Organism=Escherichia coli, GI1789194, Length=152, Percent_Identity=39.4736842105263, Blast_Score=107, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RPPH_NOVAD (Q2G7H8)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_497029.1
- ProteinModelPortal:   Q2G7H8
- SMR:   Q2G7H8
- STRING:   Q2G7H8
- GeneID:   3916330
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_1755
- eggNOG:   COG0494
- HOGENOM:   HBG302451
- OMA:   GQKQIWY
- PhylomeDB:   Q2G7H8
- BioCyc:   NARO279238:SARO_1755-MONOMER
- HAMAP:   MF_00298
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797
- InterPro:   IPR022927
- Gene3D:   G3DSA:3.90.79.10
- PRINTS:   PR00502

Pfam domain/function: PF00293 NUDIX; SSF55811 NUDIX_hydrolase

EC number: 3.6.1.- [C]

Molecular weight: Translated: 18518; Mature: 18518

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS51462 NUDIX; PS00893 NUDIX_BOX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDAFAGLPYRPCVGVMLVNSQGRVFVGRRIDDKDGVAWQMPQGGIDDGEELHPAALREL
CCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCEEEECCCCCCCCCCCCCHHHHHHH
SEETGVAAELVTIIAESREEHLYDLPDELIGKLWGGQYRGQRQKWLLLRFAGEDTDIRLD
HHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEEEC
AHDPAEFSEWRWVEPEQLPDLIVPFKRRVYRQVVDEFRDLI
CCCCCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNDAFAGLPYRPCVGVMLVNSQGRVFVGRRIDDKDGVAWQMPQGGIDDGEELHPAALREL
CCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCEEEECCCCCCCCCCCCCHHHHHHH
SEETGVAAELVTIIAESREEHLYDLPDELIGKLWGGQYRGQRQKWLLLRFAGEDTDIRLD
HHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEEEC
AHDPAEFSEWRWVEPEQLPDLIVPFKRRVYRQVVDEFRDLI
CCCCCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA