BacMap

Definition	Novosphingobium aromaticivorans DSM 12444 chromosome, complete genome.
Accession	NC_007794
Length	3,561,584

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The map label for this gene is echA12 [H]

Identifier: 87199758

GI number: 87199758

Start: 1835731

End: 1836528

Strand: Reverse

Name: echA12 [H]

Synonym: Saro_1741

Alternate gene names: 87199758

Gene position: 1836528-1835731 (Counterclockwise)

Preceding gene: 87199759

Following gene: 87199752

Centisome position: 51.56

GC content: 65.16

Gene sequence:

>798_bases
ATGGGATACGAGACCTTCACCGTGGAGAAGCGGGACGGCGCGGATTGGGTGACGCTAAACCGTCCCGAAGTGCTGAACGC
CATCTCGCTGCAGATGGTCCGCGAACTCAACGACTACTTCGGCGGCCTTTTCCATGATCGCGACACGCGCGTGGTGGTGC
TGCGCGGCGCGGGCAAGGCGTTCTGCGCGGGGCTCGACATCAAGGAGCGTGCCGAGAATCGCGACGCCATCCCGTTCGCG
GGAGGCTTCAGCTTCCAGGGCTACCTCGCGGACGTCTACATCAAGATGCGGCGCTGCCCGCAGCCCATCGTCAGCCTGGT
CCACGGGGCTGCCTGCGGCGGGGGCTTCAGCTTCGTCCTGGCATCGGACATCCGCATCGCGGGCGAAAGCGCGAAGATGA
ACGCCGCCTTCATCAAGCTTGGCCTTTCAGCGTGCGACATGGGGTGCAGCTATTTCCTGCCGCGCCTGGTCGGCACGTCG
GTTGCGAGCGAACTCATGCTGACCGGGCGATTCATCCATGCCCCGCGCGCGCTGGCCACCGGGCTGGTATCGGAAGTGGT
GCCGGATGCGGACCTCGACGCGACCGCGCAAGGTTATGTCGACGACCTGCTGGCGGCATCGCCCATGGGGCTGAGAATGA
CGAAGGAGGGCCTCAACCTCGCCATCGACGCGCCCAGCCTCGAGGCCGCGATGGCCATCGAGAACCGCAACCAGTTGATG
ACTTCGGCCAGCCCCAACTTCCGCGAGGGAATGCAGGCGTTCCTGGAGAAGCGGAAGCCGAACTACGTGGTGGAGTGA

Upstream 100 bases:

>100_bases
TCTCCATCGGGTCAGCTTGCACATTCGCACCCCTGTAGGAAAATGGTTTGAAACCGCCTCCGCCCCCGCTTAATCATTCG
ATTAAACCGGGAGATGCAGG

Downstream 100 bases:

>100_bases
GTGGCCAGCGTATCGCGCAGGAGTTCCAGCGCGGCCCTGGTGAATTGCAGCATGTTGGCAAGGCGGTCATCGCTCTGTGT
GACAACCTGCACCGTCGCCT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MGYETFTVEKRDGADWVTLNRPEVLNAISLQMVRELNDYFGGLFHDRDTRVVVLRGAGKAFCAGLDIKERAENRDAIPFA
GGFSFQGYLADVYIKMRRCPQPIVSLVHGAACGGGFSFVLASDIRIAGESAKMNAAFIKLGLSACDMGCSYFLPRLVGTS
VASELMLTGRFIHAPRALATGLVSEVVPDADLDATAQGYVDDLLAASPMGLRMTKEGLNLAIDAPSLEAAMAIENRNQLM
TSASPNFREGMQAFLEKRKPNYVVE

Sequences:

>Translated_265_residues
MGYETFTVEKRDGADWVTLNRPEVLNAISLQMVRELNDYFGGLFHDRDTRVVVLRGAGKAFCAGLDIKERAENRDAIPFA
GGFSFQGYLADVYIKMRRCPQPIVSLVHGAACGGGFSFVLASDIRIAGESAKMNAAFIKLGLSACDMGCSYFLPRLVGTS
VASELMLTGRFIHAPRALATGLVSEVVPDADLDATAQGYVDDLLAASPMGLRMTKEGLNLAIDAPSLEAAMAIENRNQLM
TSASPNFREGMQAFLEKRKPNYVVE
>Mature_264_residues
GYETFTVEKRDGADWVTLNRPEVLNAISLQMVRELNDYFGGLFHDRDTRVVVLRGAGKAFCAGLDIKERAENRDAIPFAG
GFSFQGYLADVYIKMRRCPQPIVSLVHGAACGGGFSFVLASDIRIAGESAKMNAAFIKLGLSACDMGCSYFLPRLVGTSV
ASELMLTGRFIHAPRALATGLVSEVVPDADLDATAQGYVDDLLAASPMGLRMTKEGLNLAIDAPSLEAAMAIENRNQLMT
SASPNFREGMQAFLEKRKPNYVVE

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=267, Percent_Identity=32.5842696629214, Blast_Score=114, Evalue=6e-26,
Organism=Homo sapiens, GI70995211, Length=259, Percent_Identity=32.4324324324324, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI31542718, Length=257, Percent_Identity=28.0155642023346, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI4502327, Length=261, Percent_Identity=30.6513409961686, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI37594469, Length=204, Percent_Identity=32.3529411764706, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI37594471, Length=191, Percent_Identity=32.4607329842932, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI45643119, Length=227, Percent_Identity=28.6343612334802, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI260275230, Length=227, Percent_Identity=28.6343612334802, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI260274832, Length=227, Percent_Identity=28.1938325991189, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI4757966, Length=219, Percent_Identity=27.3972602739726, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI51538581, Length=219, Percent_Identity=27.3972602739726, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI25453481, Length=219, Percent_Identity=27.3972602739726, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI51538573, Length=219, Percent_Identity=27.3972602739726, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI4757968, Length=192, Percent_Identity=28.125, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI49355787, Length=192, Percent_Identity=28.125, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI68989263, Length=171, Percent_Identity=31.5789473684211, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI221307494, Length=219, Percent_Identity=26.027397260274, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI221136756, Length=219, Percent_Identity=26.027397260274, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI221136753, Length=219, Percent_Identity=26.027397260274, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI1788597, Length=263, Percent_Identity=32.319391634981, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787660, Length=256, Percent_Identity=34.375, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI87082183, Length=270, Percent_Identity=29.2592592592593, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787659, Length=258, Percent_Identity=29.4573643410853, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI221142681, Length=252, Percent_Identity=31.3492063492063, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790281, Length=177, Percent_Identity=29.3785310734463, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788682, Length=197, Percent_Identity=29.9492385786802, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=263, Percent_Identity=33.0798479087452, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=35.2226720647773, Blast_Score=121, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17536985, Length=262, Percent_Identity=30.1526717557252, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17534483, Length=242, Percent_Identity=29.7520661157025, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17540714, Length=230, Percent_Identity=28.695652173913, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17549921, Length=212, Percent_Identity=32.5471698113208, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17535521, Length=209, Percent_Identity=29.1866028708134, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560910, Length=190, Percent_Identity=27.8947368421053, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI25144157, Length=183, Percent_Identity=32.7868852459016, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI25144160, Length=183, Percent_Identity=32.7868852459016, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=171, Percent_Identity=32.1637426900585, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=196, Percent_Identity=29.5918367346939, Blast_Score=76, Evalue=7e-15,
Organism=Drosophila melanogaster, GI20129971, Length=249, Percent_Identity=34.5381526104418, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24653477, Length=249, Percent_Identity=34.5381526104418, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI19920382, Length=222, Percent_Identity=34.2342342342342, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24653139, Length=260, Percent_Identity=34.2307692307692, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI19922422, Length=263, Percent_Identity=26.9961977186312, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21357171, Length=182, Percent_Identity=30.7692307692308, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28571730, Length=232, Percent_Identity=28.8793103448276, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI28571729, Length=232, Percent_Identity=28.8793103448276, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24654903, Length=263, Percent_Identity=23.9543726235741, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24650670, Length=258, Percent_Identity=27.906976744186, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28714; Mature: 28583

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYETFTVEKRDGADWVTLNRPEVLNAISLQMVRELNDYFGGLFHDRDTRVVVLRGAGKA
CCCCEEEEECCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCEEECCCCEEEEEECCCCC
FCAGLDIKERAENRDAIPFAGGFSFQGYLADVYIKMRRCPQPIVSLVHGAACGGGFSFVL
EEECCCHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEE
ASDIRIAGESAKMNAAFIKLGLSACDMGCSYFLPRLVGTSVASELMLTGRFIHAPRALAT
ECCEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
GLVSEVVPDADLDATAQGYVDDLLAASPMGLRMTKEGLNLAIDAPSLEAAMAIENRNQLM
HHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCEEEEECCCCHHHHHHHCCHHHH
TSASPNFREGMQAFLEKRKPNYVVE
HCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GYETFTVEKRDGADWVTLNRPEVLNAISLQMVRELNDYFGGLFHDRDTRVVVLRGAGKA
CCCEEEEECCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCEEECCCCEEEEEECCCCC
FCAGLDIKERAENRDAIPFAGGFSFQGYLADVYIKMRRCPQPIVSLVHGAACGGGFSFVL
EEECCCHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEE
ASDIRIAGESAKMNAAFIKLGLSACDMGCSYFLPRLVGTSVASELMLTGRFIHAPRALAT
ECCEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
GLVSEVVPDADLDATAQGYVDDLLAASPMGLRMTKEGLNLAIDAPSLEAAMAIENRNQLM
HHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCEEEEECCCCHHHHHHHCCHHHH
TSASPNFREGMQAFLEKRKPNYVVE
HCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]