Click here to switch to the map view.

The map label for this gene is emrA [H]

Identifier: 87199732

GI number: 87199732

Start: 1803083

End: 1804234

Strand: Reverse

Name: emrA [H]

Synonym: Saro_1715

Alternate gene names: 87199732

Gene position: 1804234-1803083 (Counterclockwise)

Preceding gene: 87199733

Following gene: 87199731

Centisome position: 50.66

GC content: 66.15

Gene sequence:

>1152_bases
ATGACAATGGCCGCCACCCCCGATTCTCTTCCCGCCAACGCCGATGCCCCCGCCGAGAACGAAGGGCGCAAGAAGACGGT
GGGACGCATCGCACTGGGCGTGCTCGCGCTCGGCGCAGCCTATGGCGCCTATGCGCTCTACCACTACGAGACCGTCGGCC
AGTACATGCAGGAAACCAATGACGCTTATGTGAAGGCAGATGGCGTCACCGTAAGCTCGAAGCTGGCAGGCTATGTCCGG
ACCGTCGCCGTGGCCGACAACCAGTCGGTCAACGACGGCGCGCTCCTCGTACAGATCGATCCTACCGATTACGACACGCG
CCTCGCACAGAGCACCGCACAAGTCGAAGTTGCCCGTGCGACCGAAACAGCAACGCTTGCCGCCATCAACGAAGCGCAAT
CGGCGGTCGGACAGGCGCGGGCCGCGCTCCAGGCCTCGCAACGCGAGCTTGCCTACCTCAACGGCGAAGTCGCCCGGATG
CGCCCGCTGGTCGCCAGCGGCGCCGAACCCCGGCAGGCATTCGACCAGCTTGTCGCCAACCGCGACAAGGCTGCTGCCGA
CGTTTCGGCCAAGCAGGCCGCGCTTTCGGCCGCAAGCGACCGCGTCGCCAGCACCCGGGCGCAGGCGGGGCAATCCGCCG
CACAGGTCAAGGCCGCGCAAGCCCAGCGCCAGGCCGCCAGCACCGATCTTGCTACCACCCGCATCGTCGCTCCCGTTTCC
GGCAAAATCGGCAATTCGACGGTCCGCGTCGGGCAATTCGTGCAACCGGGCCAGCGCCTGATGACGATCGTTCCCACACA
GGCCATCTATGTCGAGGCCAACTTCAAGGAAACGCAGATCGGCCTGATGCGCGCGGGACAGCCGGTGACGGTGCACGTGG
ATGCCCTGCCCGACGTCGATTTCCACGGTACGGTCGAAAGCATCACCCCCGGCACGGGCGCGAACTTCTCGTTGATCCCG
CCGCAGAACGCGACCGGCAACTTCACCAAGATCGTCCAGCGCGTACCGGTGCGCATCCGCATCAACGCCGGCCCCGAATC
GCGCAAGGTGCTGGTGCCGGGCCTGTCGCTTACGGTCGAGGTGGATACCCGCTCGGCGAAGTCGGCCATCGACGCGATCC
GCGATGAACAGGACCGGGTCTCGGCCAAGTGA

Upstream 100 bases:

>100_bases
TGCACCGCCCCTGTCAATTTCCGCACTTGAAAAGCTGACGCGGTTGAACCAAGTGGCGATCTGCAACGAAACGATTTCGT
TCAATTCTTTCGAAACTGGA

Downstream 100 bases:

>100_bases
CAGCGGCAATCTCTTCCGACGCCGACACCGAAGACCTGGCGCTGCCGCCCCCCCGGGCGCGCGAGAAGGCGGACCTGACC
GCTTGGCTGGGCGTGGCCGC

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MTMAATPDSLPANADAPAENEGRKKTVGRIALGVLALGAAYGAYALYHYETVGQYMQETNDAYVKADGVTVSSKLAGYVR
TVAVADNQSVNDGALLVQIDPTDYDTRLAQSTAQVEVARATETATLAAINEAQSAVGQARAALQASQRELAYLNGEVARM
RPLVASGAEPRQAFDQLVANRDKAAADVSAKQAALSAASDRVASTRAQAGQSAAQVKAAQAQRQAASTDLATTRIVAPVS
GKIGNSTVRVGQFVQPGQRLMTIVPTQAIYVEANFKETQIGLMRAGQPVTVHVDALPDVDFHGTVESITPGTGANFSLIP
PQNATGNFTKIVQRVPVRIRINAGPESRKVLVPGLSLTVEVDTRSAKSAIDAIRDEQDRVSAK

Sequences:

>Translated_383_residues
MTMAATPDSLPANADAPAENEGRKKTVGRIALGVLALGAAYGAYALYHYETVGQYMQETNDAYVKADGVTVSSKLAGYVR
TVAVADNQSVNDGALLVQIDPTDYDTRLAQSTAQVEVARATETATLAAINEAQSAVGQARAALQASQRELAYLNGEVARM
RPLVASGAEPRQAFDQLVANRDKAAADVSAKQAALSAASDRVASTRAQAGQSAAQVKAAQAQRQAASTDLATTRIVAPVS
GKIGNSTVRVGQFVQPGQRLMTIVPTQAIYVEANFKETQIGLMRAGQPVTVHVDALPDVDFHGTVESITPGTGANFSLIP
PQNATGNFTKIVQRVPVRIRINAGPESRKVLVPGLSLTVEVDTRSAKSAIDAIRDEQDRVSAK
>Mature_382_residues
TMAATPDSLPANADAPAENEGRKKTVGRIALGVLALGAAYGAYALYHYETVGQYMQETNDAYVKADGVTVSSKLAGYVRT
VAVADNQSVNDGALLVQIDPTDYDTRLAQSTAQVEVARATETATLAAINEAQSAVGQARAALQASQRELAYLNGEVARMR
PLVASGAEPRQAFDQLVANRDKAAADVSAKQAALSAASDRVASTRAQAGQSAAQVKAAQAQRQAASTDLATTRIVAPVSG
KIGNSTVRVGQFVQPGQRLMTIVPTQAIYVEANFKETQIGLMRAGQPVTVHVDALPDVDFHGTVESITPGTGANFSLIPP
QNATGNFTKIVQRVPVRIRINAGPESRKVLVPGLSLTVEVDTRSAKSAIDAIRDEQDRVSAK

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788711, Length=336, Percent_Identity=33.9285714285714, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1789041, Length=353, Percent_Identity=32.5779036827195, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1790519, Length=305, Percent_Identity=28.1967213114754, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87081951, Length=346, Percent_Identity=27.7456647398844, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1789900, Length=295, Percent_Identity=25.0847457627119, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790024, Length=249, Percent_Identity=25.7028112449799, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787013, Length=247, Percent_Identity=26.3157894736842, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40195; Mature: 40063

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMAATPDSLPANADAPAENEGRKKTVGRIALGVLALGAAYGAYALYHYETVGQYMQETN
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DAYVKADGVTVSSKLAGYVRTVAVADNQSVNDGALLVQIDPTDYDTRLAQSTAQVEVARA
CCEEEECCEEEHHHHHHHEEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHEEEECC
TETATLAAINEAQSAVGQARAALQASQRELAYLNGEVARMRPLVASGAEPRQAFDQLVAN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHC
RDKAAADVSAKQAALSAASDRVASTRAQAGQSAAQVKAAQAQRQAASTDLATTRIVAPVS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCC
GKIGNSTVRVGQFVQPGQRLMTIVPTQAIYVEANFKETQIGLMRAGQPVTVHVDALPDVD
CCCCCCEEEECCCCCCCCCEEEEECCEEEEEEECCCHHEEHHEECCCCEEEEEECCCCCC
FHGTVESITPGTGANFSLIPPQNATGNFTKIVQRVPVRIRINAGPESRKVLVPGLSLTVE
CCCCHHHCCCCCCCCEEEECCCCCCCHHHHHHHHCCEEEEECCCCCCCEEEECCCEEEEE
VDTRSAKSAIDAIRDEQDRVSAK
ECCCHHHHHHHHHHCCHHHCCCC
>Mature Secondary Structure 
TMAATPDSLPANADAPAENEGRKKTVGRIALGVLALGAAYGAYALYHYETVGQYMQETN
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DAYVKADGVTVSSKLAGYVRTVAVADNQSVNDGALLVQIDPTDYDTRLAQSTAQVEVARA
CCEEEECCEEEHHHHHHHEEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHEEEECC
TETATLAAINEAQSAVGQARAALQASQRELAYLNGEVARMRPLVASGAEPRQAFDQLVAN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHC
RDKAAADVSAKQAALSAASDRVASTRAQAGQSAAQVKAAQAQRQAASTDLATTRIVAPVS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCC
GKIGNSTVRVGQFVQPGQRLMTIVPTQAIYVEANFKETQIGLMRAGQPVTVHVDALPDVD
CCCCCCEEEECCCCCCCCCEEEEECCEEEEEEECCCHHEEHHEECCCCEEEEEECCCCCC
FHGTVESITPGTGANFSLIPPQNATGNFTKIVQRVPVRIRINAGPESRKVLVPGLSLTVE
CCCCHHHCCCCCCCCEEEECCCCCCCHHHHHHHHCCEEEEECCCCCCCEEEECCCEEEEE
VDTRSAKSAIDAIRDEQDRVSAK
ECCCHHHHHHHHHHCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]