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The map label for this gene is puuB [H]

Identifier: 87199557

GI number: 87199557

Start: 1587982

End: 1589310

Strand: Reverse

Name: puuB [H]

Synonym: Saro_1539

Alternate gene names: 87199557

Gene position: 1589310-1587982 (Counterclockwise)

Preceding gene: 87199558

Following gene: 87199552

Centisome position: 44.62

GC content: 64.86

Gene sequence:

>1329_bases
ATGAATTTACAAATGCCGTCGAACGCCGACTTCGAACGCGGCGAGGTGCCCGTCACGCTCAGCGGATGGACGATGCCGCA
AGGCATCTCGCACCCCTGGCCCGTTCTCGAAGGAGATACCGAGACGAGTGTTGCCGTCATCGGGGCTGGTCTTGCCGGTT
CGTCTCTGGCGCTTCATCTGGCCGAAGCGGGTGTTGCGGTGACGGTGATCGAAGCGCGGCAGCCGGGCTGGGGTGCGTCG
GGTCGCAATGCGGGCCACGTACTGCCGATCCTGCGCGACCTGAACGTCCTCGAGAAGTACACTGACGGTGGTCGACGGTT
CCTTGAGCTGTTCCGGGAACACCTGACCATCCCCTACGACCTGTCGCGCCGCCACGGGATCGACTGCGACGCCGTGCGCA
GTGGCTACGTCAACGGCATGCGCAGCGAGCGCGCGTTGGACGCCTTCCGGCGCCAGCATGGCGATCTGGAGCGCGCCGGC
ATCCAGCGCCTTGTCCCCTTGTCGCCGGAGGAGATGCACCGCCGCCTCGGCACCGGGGCCTACCCCTACGGCGTGTTGTT
CGAGGACGGTGGGCGGGTGAACCCCTATCTGTTTACCAACGGCATGATCGCGGCCGCTGCACGTATGGGCACGCGGGTTC
ATGGAGACAGCGAAGCGCTGTCGATCAACCGGACCGGCTCTCGCTGGTGCGTGCGTACCGCGCATGGAAGCGTGACTGCG
GACAGGGTCGTGTTCTGCACCAATGCCTATCCGGGAAAGGCAGTCCCCGCCCTGCAGGACGCATTCTACCCGCTGACCGC
CTATGCGATCACCACTCGCCCCCTGCCGCCGGAGGCGCTCGAGCTTGTGCTGCCCGGCGGCGGCACTTTCGCGCAGGTGC
CGGTCGACCTGAATCCGATCGTACGCGATCGGCACCACCGCCTGATCCTGTCTTCGATCCCTCGCAGTGGCGGAGCAAAG
GATGCGGATTGGCACTTCCGTTCGCAACTTCGCTGGCTGCATCGGACATGGCCTGCCACGCGCGGGATGACGATCGAGAT
GGAGACATACTGGACCGGGCGAGTGGCGATGCGCGACCGTCAATTTCCGGGCGTTTTCGAACTTCAGGATGGCGTCTACG
GCCTCATGTATTTCAACGCCTGGGGCAATGTCATGGCCCCTCTCATGGGCAAGCTTCTGGCCGACGCCCTGGCCAAGGAC
GATCCAGGTCGCCTGCCCTTCCCGCTCGAAACGCCCGAGCCCGTCTCCATGCCTTGCAAGCAGGAATTGCTCATCCGGCA
TCTTCTGATCCCGGCCGCTCGCACCGCTCAGCGGCTAGGCATCATCTGA

Upstream 100 bases:

>100_bases
CCATTTGGCCTGTTCGCCGCGTGCCTTCTGGCGGCGGCCCTTGCCTGTCGCCTTGGCTCCATGAGCCAGCCCAATTCCAT
GCATGCGTGAGCCGATTTCC

Downstream 100 bases:

>100_bases
CCAGGGGCATCAACTATTTGCCGTCTGACTAGCGCCAGCAATGACGTTGACATGGGAATGGGGATTTCGCTGAATTCGGG
GCGTCAGCGAAAATCCCGGG

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MNLQMPSNADFERGEVPVTLSGWTMPQGISHPWPVLEGDTETSVAVIGAGLAGSSLALHLAEAGVAVTVIEARQPGWGAS
GRNAGHVLPILRDLNVLEKYTDGGRRFLELFREHLTIPYDLSRRHGIDCDAVRSGYVNGMRSERALDAFRRQHGDLERAG
IQRLVPLSPEEMHRRLGTGAYPYGVLFEDGGRVNPYLFTNGMIAAAARMGTRVHGDSEALSINRTGSRWCVRTAHGSVTA
DRVVFCTNAYPGKAVPALQDAFYPLTAYAITTRPLPPEALELVLPGGGTFAQVPVDLNPIVRDRHHRLILSSIPRSGGAK
DADWHFRSQLRWLHRTWPATRGMTIEMETYWTGRVAMRDRQFPGVFELQDGVYGLMYFNAWGNVMAPLMGKLLADALAKD
DPGRLPFPLETPEPVSMPCKQELLIRHLLIPAARTAQRLGII

Sequences:

>Translated_442_residues
MNLQMPSNADFERGEVPVTLSGWTMPQGISHPWPVLEGDTETSVAVIGAGLAGSSLALHLAEAGVAVTVIEARQPGWGAS
GRNAGHVLPILRDLNVLEKYTDGGRRFLELFREHLTIPYDLSRRHGIDCDAVRSGYVNGMRSERALDAFRRQHGDLERAG
IQRLVPLSPEEMHRRLGTGAYPYGVLFEDGGRVNPYLFTNGMIAAAARMGTRVHGDSEALSINRTGSRWCVRTAHGSVTA
DRVVFCTNAYPGKAVPALQDAFYPLTAYAITTRPLPPEALELVLPGGGTFAQVPVDLNPIVRDRHHRLILSSIPRSGGAK
DADWHFRSQLRWLHRTWPATRGMTIEMETYWTGRVAMRDRQFPGVFELQDGVYGLMYFNAWGNVMAPLMGKLLADALAKD
DPGRLPFPLETPEPVSMPCKQELLIRHLLIPAARTAQRLGII
>Mature_442_residues
MNLQMPSNADFERGEVPVTLSGWTMPQGISHPWPVLEGDTETSVAVIGAGLAGSSLALHLAEAGVAVTVIEARQPGWGAS
GRNAGHVLPILRDLNVLEKYTDGGRRFLELFREHLTIPYDLSRRHGIDCDAVRSGYVNGMRSERALDAFRRQHGDLERAG
IQRLVPLSPEEMHRRLGTGAYPYGVLFEDGGRVNPYLFTNGMIAAAARMGTRVHGDSEALSINRTGSRWCVRTAHGSVTA
DRVVFCTNAYPGKAVPALQDAFYPLTAYAITTRPLPPEALELVLPGGGTFAQVPVDLNPIVRDRHHRLILSSIPRSGGAK
DADWHFRSQLRWLHRTWPATRGMTIEMETYWTGRVAMRDRQFPGVFELQDGVYGLMYFNAWGNVMAPLMGKLLADALAKD
DPGRLPFPLETPEPVSMPCKQELLIRHLLIPAARTAQRLGII

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=373, Percent_Identity=30.2949061662198, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 48737; Mature: 48737

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLQMPSNADFERGEVPVTLSGWTMPQGISHPWPVLEGDTETSVAVIGAGLAGSSLALHL
CCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHEEEE
AEAGVAVTVIEARQPGWGASGRNAGHVLPILRDLNVLEKYTDGGRRFLELFREHLTIPYD
ECCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
LSRRHGIDCDAVRSGYVNGMRSERALDAFRRQHGDLERAGIQRLVPLSPEEMHRRLGTGA
CCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHCCCC
YPYGVLFEDGGRVNPYLFTNGMIAAAARMGTRVHGDSEALSINRTGSRWCVRTAHGSVTA
CCCEEEECCCCCCCCEEEECCHHHHHHHHCCEECCCCCEEEEECCCCEEEEEECCCCCCC
DRVVFCTNAYPGKAVPALQDAFYPLTAYAITTRPLPPEALELVLPGGGTFAQVPVDLNPI
CEEEEEECCCCCCCCHHHHHCCCCEEEEEEECCCCCHHHEEEEECCCCCEEECCCCCCHH
VRDRHHRLILSSIPRSGGAKDADWHFRSQLRWLHRTWPATRGMTIEMETYWTGRVAMRDR
HHCCHHEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEECCEEEEECC
QFPGVFELQDGVYGLMYFNAWGNVMAPLMGKLLADALAKDDPGRLPFPLETPEPVSMPCK
CCCCEEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
QELLIRHLLIPAARTAQRLGII
HHHHHHHHHHCCHHHHHHHCCC
>Mature Secondary Structure
MNLQMPSNADFERGEVPVTLSGWTMPQGISHPWPVLEGDTETSVAVIGAGLAGSSLALHL
CCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHEEEE
AEAGVAVTVIEARQPGWGASGRNAGHVLPILRDLNVLEKYTDGGRRFLELFREHLTIPYD
ECCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
LSRRHGIDCDAVRSGYVNGMRSERALDAFRRQHGDLERAGIQRLVPLSPEEMHRRLGTGA
CCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHCCCC
YPYGVLFEDGGRVNPYLFTNGMIAAAARMGTRVHGDSEALSINRTGSRWCVRTAHGSVTA
CCCEEEECCCCCCCCEEEECCHHHHHHHHCCEECCCCCEEEEECCCCEEEEEECCCCCCC
DRVVFCTNAYPGKAVPALQDAFYPLTAYAITTRPLPPEALELVLPGGGTFAQVPVDLNPI
CEEEEEECCCCCCCCHHHHHCCCCEEEEEEECCCCCHHHEEEEECCCCCEEECCCCCCHH
VRDRHHRLILSSIPRSGGAKDADWHFRSQLRWLHRTWPATRGMTIEMETYWTGRVAMRDR
HHCCHHEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEECCEEEEECC
QFPGVFELQDGVYGLMYFNAWGNVMAPLMGKLLADALAKDDPGRLPFPLETPEPVSMPCK
CCCCEEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
QELLIRHLLIPAARTAQRLGII
HHHHHHHHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]