Click here to switch to the map view.

The map label for this gene is proB

Identifier: 87199168

GI number: 87199168

Start: 1194468

End: 1195601

Strand: Direct

Name: proB

Synonym: Saro_1146

Alternate gene names: 87199168

Gene position: 1194468-1195601 (Clockwise)

Preceding gene: 161760696

Following gene: 87199169

Centisome position: 33.54

GC content: 69.4

Gene sequence:

>1134_bases
ATGAAGATCAGCCAGCTTGCCCAACTGACCCAGGCTTCCACCTGTCCGCGCCTCGTGGTCAAGGTCGGGTCGGCCCTGCT
TGTCGGCAAGGATGGCGAGCCGCGCCGCGAATGGCTTTCTGCCCTGGTGTCCGAGATCGCCGCCATGCGCGCGGCCGGAC
AGGAAGTCATCGTCGTCTCCTCCGGCGCGATCGCGCTCGGTGCGCGCAAGCTCGGGCTTGCCAAGGGCGGGCGGGGCAGC
CTCTCCGACGCCCAGGCCGCCGCCTCGGTGGGCCAGATCGCGCTCGCGGGGCTCTGGGCCGAACTTCTGGCGCAGCACGG
GCTCACCGCCGCGCAGATCCTGCTGACGCTCGAAGATCTCGAGGATCGCCGACGTTACCTCAATGTCACCGCCACGCTGG
GCACGCTGCTCGCGGCATGCGCGGTGCCGGTGATCAACGAGAACGATTCCGTCGCAACCCAGGAAATCCGTTTCGGAGAC
AACGACCGCCTTGCCGCCCGTGTGGGCCAGGCCGCAGGCGCATCGGGTGTGCTGCTCCTGTCCGACATCGACGGTCTCTA
CGATCGCGACCCCCGCCAGCCCGGCGCCACCCGCATTCCCGTGGTCAAGGGCGTGACCCCTGAAATCCACGCCATGGCCA
CTGGCGGTTCGTCTTCCGGACTCGGCTCGGGCGGCATGACGTCCAAGCTCCAGGCCGCCGAAATTGCCGAGCTTGCCGGA
ATGGCGCTTGCCATCATCGACGGACAGCCCGTTGCCCCGATCGCCGCTGCCATGGGCGCTGCGCGCGGCACCCTGTTCCT
GCCACGCGGACGCAAGCAGGCGCGCAAGGCCTGGCTCGGCGGGCGAATGCGGATGCGCGGCAGCGTGCAGGTCGACGCCG
GTGCCGCCGCCGCGCTGGCCCGTGGCTCCAGCCTGCTTGCCGCCGGAGTGACAGAGGTCGACGGCGATTTCCAGCGCGGC
GATGCCATTGCCGTGCTCGGTCCCGACGGCCGGACGCTGGCGCGCGGCCTTTCGGAATACGACGCTGCCGAGTGCGCGCG
CCTCAAGGGGCGCCACAGCCGCGAGCATGAAGAACTTCTGGGCTATGCGCCGCGCTCGGCATTGATCCATCGCGACCAGA
TGGTGCTGCTGTAA

Upstream 100 bases:

>100_bases
GAGGGACGCGGCGGCCACGCTTGGCGTCATAGACACGACGCCCTGCATATCCGACCGGTAACCCTTCACGCACCGCCACA
TTTGGCCTAAGCGGCTCCCC

Downstream 100 bases:

>100_bases
TGGCGGCAGAATTGCTGGCGATCACCGGTGCCACCGGGTTCGTCGGGCAGGCGGTTCTCGATTTCGCCGCCCGCGCAGGG
ATAGAAGTCCGTGCGCTTGC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MKISQLAQLTQASTCPRLVVKVGSALLVGKDGEPRREWLSALVSEIAAMRAAGQEVIVVSSGAIALGARKLGLAKGGRGS
LSDAQAAASVGQIALAGLWAELLAQHGLTAAQILLTLEDLEDRRRYLNVTATLGTLLAACAVPVINENDSVATQEIRFGD
NDRLAARVGQAAGASGVLLLSDIDGLYDRDPRQPGATRIPVVKGVTPEIHAMATGGSSSGLGSGGMTSKLQAAEIAELAG
MALAIIDGQPVAPIAAAMGAARGTLFLPRGRKQARKAWLGGRMRMRGSVQVDAGAAAALARGSSLLAAGVTEVDGDFQRG
DAIAVLGPDGRTLARGLSEYDAAECARLKGRHSREHEELLGYAPRSALIHRDQMVLL

Sequences:

>Translated_377_residues
MKISQLAQLTQASTCPRLVVKVGSALLVGKDGEPRREWLSALVSEIAAMRAAGQEVIVVSSGAIALGARKLGLAKGGRGS
LSDAQAAASVGQIALAGLWAELLAQHGLTAAQILLTLEDLEDRRRYLNVTATLGTLLAACAVPVINENDSVATQEIRFGD
NDRLAARVGQAAGASGVLLLSDIDGLYDRDPRQPGATRIPVVKGVTPEIHAMATGGSSSGLGSGGMTSKLQAAEIAELAG
MALAIIDGQPVAPIAAAMGAARGTLFLPRGRKQARKAWLGGRMRMRGSVQVDAGAAAALARGSSLLAAGVTEVDGDFQRG
DAIAVLGPDGRTLARGLSEYDAAECARLKGRHSREHEELLGYAPRSALIHRDQMVLL
>Mature_377_residues
MKISQLAQLTQASTCPRLVVKVGSALLVGKDGEPRREWLSALVSEIAAMRAAGQEVIVVSSGAIALGARKLGLAKGGRGS
LSDAQAAASVGQIALAGLWAELLAQHGLTAAQILLTLEDLEDRRRYLNVTATLGTLLAACAVPVINENDSVATQEIRFGD
NDRLAARVGQAAGASGVLLLSDIDGLYDRDPRQPGATRIPVVKGVTPEIHAMATGGSSSGLGSGGMTSKLQAAEIAELAG
MALAIIDGQPVAPIAAAMGAARGTLFLPRGRKQARKAWLGGRMRMRGSVQVDAGAAAALARGSSLLAAGVTEVDGDFQRG
DAIAVLGPDGRTLARGLSEYDAAECARLKGRHSREHEELLGYAPRSALIHRDQMVLL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=321, Percent_Identity=28.9719626168224, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI62912457, Length=319, Percent_Identity=29.153605015674, Blast_Score=112, Evalue=8e-25,
Organism=Escherichia coli, GI1786437, Length=367, Percent_Identity=42.7792915531335, Blast_Score=244, Evalue=6e-66,
Organism=Caenorhabditis elegans, GI17569969, Length=271, Percent_Identity=32.1033210332103, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17569967, Length=271, Percent_Identity=32.1033210332103, Blast_Score=128, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6320506, Length=415, Percent_Identity=32.5301204819277, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6321822, Length=426, Percent_Identity=29.5774647887324, Blast_Score=160, Evalue=3e-40,
Organism=Drosophila melanogaster, GI21357643, Length=296, Percent_Identity=30.7432432432432, Blast_Score=122, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_NOVAD (Q2G982)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_496425.1
- ProteinModelPortal:   Q2G982
- SMR:   Q2G982
- STRING:   Q2G982
- GeneID:   3916443
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_1146
- NMPDR:   fig|48935.1.peg.2032
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- PhylomeDB:   Q2G982
- ProtClustDB:   PRK05429
- BioCyc:   NARO279238:SARO_1146-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 38909; Mature: 38909

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISQLAQLTQASTCPRLVVKVGSALLVGKDGEPRREWLSALVSEIAAMRAAGQEVIVVS
CCHHHHHHHHHHCCCHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEE
SGAIALGARKLGLAKGGRGSLSDAQAAASVGQIALAGLWAELLAQHGLTAAQILLTLEDL
CCCEEECHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EDRRRYLNVTATLGTLLAACAVPVINENDSVATQEIRFGDNDRLAARVGQAAGASGVLLL
HHHHHHHHHHHHHHHHHHHHHCCEECCCCCCEEEEEECCCCCHHHHHHHHCCCCCCEEEE
SDIDGLYDRDPRQPGATRIPVVKGVTPEIHAMATGGSSSGLGSGGMTSKLQAAEIAELAG
ECCCCCCCCCCCCCCCCCCCEEECCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHC
MALAIIDGQPVAPIAAAMGAARGTLFLPRGRKQARKAWLGGRMRMRGSVQVDAGAAAALA
CEEEEECCCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCEEECCCEEECCCHHHHHH
RGSSLLAAGVTEVDGDFQRGDAIAVLGPDGRTLARGLSEYDAAECARLKGRHSREHEELL
CCCHHHCCCCHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
GYAPRSALIHRDQMVLL
CCCCHHHHHCCCCEEEC
>Mature Secondary Structure
MKISQLAQLTQASTCPRLVVKVGSALLVGKDGEPRREWLSALVSEIAAMRAAGQEVIVVS
CCHHHHHHHHHHCCCHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEE
SGAIALGARKLGLAKGGRGSLSDAQAAASVGQIALAGLWAELLAQHGLTAAQILLTLEDL
CCCEEECHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EDRRRYLNVTATLGTLLAACAVPVINENDSVATQEIRFGDNDRLAARVGQAAGASGVLLL
HHHHHHHHHHHHHHHHHHHHHCCEECCCCCCEEEEEECCCCCHHHHHHHHCCCCCCEEEE
SDIDGLYDRDPRQPGATRIPVVKGVTPEIHAMATGGSSSGLGSGGMTSKLQAAEIAELAG
ECCCCCCCCCCCCCCCCCCCEEECCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHC
MALAIIDGQPVAPIAAAMGAARGTLFLPRGRKQARKAWLGGRMRMRGSVQVDAGAAAALA
CEEEEECCCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCEEECCCEEECCCHHHHHH
RGSSLLAAGVTEVDGDFQRGDAIAVLGPDGRTLARGLSEYDAAECARLKGRHSREHEELL
CCCHHHCCCCHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
GYAPRSALIHRDQMVLL
CCCCHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA