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The map label for this gene is ddl

Identifier: 87199158

GI number: 87199158

Start: 1182477

End: 1183418

Strand: Direct

Name: ddl

Synonym: Saro_1136

Alternate gene names: 87199158

Gene position: 1182477-1183418 (Clockwise)

Preceding gene: 87199157

Following gene: 87199159

Centisome position: 33.2

GC content: 64.97

Gene sequence:

>942_bases
GTGTCCCTGCCCAAACTTCACGTTGCAGTCCTCATGGGTGGCTGGTCCAGCGAACGCCCCGTGTCGCTCATGTCCGGCGA
GGGCGTGGCCAAGGCTCTTGAATCGAAGGGGCACCAAGTCACGCGCATCGACATGGACCGCGACGTCGCACTGCGCCTGG
CCGAGGCGAAGCCTGACGTCGTGTTCAATGCCCTGCACGGCACCCCGGGCGAGGATGGTTCGATCCAGGGCCTGATGGAC
ATCATGGGCCTGACCTATACCCACTCCGGCCTGGTCACCTCGGTCATCGCGATCGATAAGGAACTGACCAAGCAGGCGCT
GGTGCCACATGGCATCCCGATGCCCGGCGGGCGCATGGTCAAGACGGCTGACCTCTACAAGGCCGATCCGCTGCCGCGCC
CCTATGTGCTGAAGCCCGTCAACGAAGGGTCATCGGTCGGGGTCGCGATCGTCACTGCCGAGGGCAATTACGGCAGCCCG
ATCAGCGCGGCGAGCAAGGGCCCGTGGCAGGAATTCGACCAGCTCCTCGCCGAACCCTTCATCCGCGGGCGCGAGCTGAC
GACCGCCGTGATCGGCGACCGCGCGCTTCTGGTCACCGAACTTCGTCCGAAGTCCGGCTTCTACGACTTCGACGCCAAGT
ACACCGAGGGCATGACCGACCACATCTGTCCCGCCGAGATTCCCGACGAGATCACCGAGGCGTGCAAGGACATTGCGCTG
CGGGCCCACAGGTTGCTCGGCTGCAAGGGGACCAGTCGTTCCGACTTCCGCTGGGATGACGAGCAGGGCGTCGAGGGACT
GTTCCTGCTGGAAGTGAACACCCAGCCGGGCATGACGCCGCTCAGCCTCGTGCCGGAACAGGCGCGGGCGCTCGGCATGG
ATTATTCCGATCTGGTGGAGGCGATCATCGCCGAAGCCCTGAAGGACGCAGGAAAGGCCTGA

Upstream 100 bases:

>100_bases
AAGGCGAGTTCAGGCGTGGACCTCGAATGGGAAATCAAGAGGATAGGCCGGCCATGACGGTGCCCGCCCATCGACTCGAG
CCGAACGGAGAATGCTGAGA

Downstream 100 bases:

>100_bases
ACCCATGGCGCAGACGATCAAGCGTGGCGGAAAGGGCGTGCGGCGCGCGACCGCAGCGCGTTCGGCGCAGCGCAAGGTCC
AGACCGCGCGCCAGCAGACC

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSLPKLHVAVLMGGWSSERPVSLMSGEGVAKALESKGHQVTRIDMDRDVALRLAEAKPDVVFNALHGTPGEDGSIQGLMD
IMGLTYTHSGLVTSVIAIDKELTKQALVPHGIPMPGGRMVKTADLYKADPLPRPYVLKPVNEGSSVGVAIVTAEGNYGSP
ISAASKGPWQEFDQLLAEPFIRGRELTTAVIGDRALLVTELRPKSGFYDFDAKYTEGMTDHICPAEIPDEITEACKDIAL
RAHRLLGCKGTSRSDFRWDDEQGVEGLFLLEVNTQPGMTPLSLVPEQARALGMDYSDLVEAIIAEALKDAGKA

Sequences:

>Translated_313_residues
MSLPKLHVAVLMGGWSSERPVSLMSGEGVAKALESKGHQVTRIDMDRDVALRLAEAKPDVVFNALHGTPGEDGSIQGLMD
IMGLTYTHSGLVTSVIAIDKELTKQALVPHGIPMPGGRMVKTADLYKADPLPRPYVLKPVNEGSSVGVAIVTAEGNYGSP
ISAASKGPWQEFDQLLAEPFIRGRELTTAVIGDRALLVTELRPKSGFYDFDAKYTEGMTDHICPAEIPDEITEACKDIAL
RAHRLLGCKGTSRSDFRWDDEQGVEGLFLLEVNTQPGMTPLSLVPEQARALGMDYSDLVEAIIAEALKDAGKA
>Mature_312_residues
SLPKLHVAVLMGGWSSERPVSLMSGEGVAKALESKGHQVTRIDMDRDVALRLAEAKPDVVFNALHGTPGEDGSIQGLMDI
MGLTYTHSGLVTSVIAIDKELTKQALVPHGIPMPGGRMVKTADLYKADPLPRPYVLKPVNEGSSVGVAIVTAEGNYGSPI
SAASKGPWQEFDQLLAEPFIRGRELTTAVIGDRALLVTELRPKSGFYDFDAKYTEGMTDHICPAEIPDEITEACKDIALR
AHRLLGCKGTSRSDFRWDDEQGVEGLFLLEVNTQPGMTPLSLVPEQARALGMDYSDLVEAIIAEALKDAGKA

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=305, Percent_Identity=36.0655737704918, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1786579, Length=360, Percent_Identity=30.5555555555556, Blast_Score=144, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_NOVAD (Q2G992)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_496415.1
- ProteinModelPortal:   Q2G992
- SMR:   Q2G992
- STRING:   Q2G992
- GeneID:   3916432
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_1136
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   TDGWYDY
- PhylomeDB:   Q2G992
- ProtClustDB:   PRK01372
- BioCyc:   NARO279238:SARO_1136-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 33694; Mature: 33563

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPKLHVAVLMGGWSSERPVSLMSGEGVAKALESKGHQVTRIDMDRDVALRLAEAKPDV
CCCCCEEEEEEECCCCCCCCEEEECCCHHHHHHHHCCCEEEEEECCCCHHHEECCCCCCE
VFNALHGTPGEDGSIQGLMDIMGLTYTHSGLVTSVIAIDKELTKQALVPHGIPMPGGRMV
EEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
KTADLYKADPLPRPYVLKPVNEGSSVGVAIVTAEGNYGSPISAASKGPWQEFDQLLAEPF
EEHHHHCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
IRGRELTTAVIGDRALLVTELRPKSGFYDFDAKYTEGMTDHICPAEIPDEITEACKDIAL
HCCCCEEEEECCCCEEEEEEECCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHH
RAHRLLGCKGTSRSDFRWDDEQGVEGLFLLEVNTQPGMTPLSLVPEQARALGMDYSDLVE
HHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHCCHHHHHHCCCHHHHHH
AIIAEALKDAGKA
HHHHHHHHHCCCC
>Mature Secondary Structure 
SLPKLHVAVLMGGWSSERPVSLMSGEGVAKALESKGHQVTRIDMDRDVALRLAEAKPDV
CCCCEEEEEEECCCCCCCCEEEECCCHHHHHHHHCCCEEEEEECCCCHHHEECCCCCCE
VFNALHGTPGEDGSIQGLMDIMGLTYTHSGLVTSVIAIDKELTKQALVPHGIPMPGGRMV
EEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
KTADLYKADPLPRPYVLKPVNEGSSVGVAIVTAEGNYGSPISAASKGPWQEFDQLLAEPF
EEHHHHCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
IRGRELTTAVIGDRALLVTELRPKSGFYDFDAKYTEGMTDHICPAEIPDEITEACKDIAL
HCCCCEEEEECCCCEEEEEEECCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHH
RAHRLLGCKGTSRSDFRWDDEQGVEGLFLLEVNTQPGMTPLSLVPEQARALGMDYSDLVE
HHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHCCHHHHHHCCCHHHHHH
AIIAEALKDAGKA
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA