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The map label for this gene is murD

Identifier: 87199153

GI number: 87199153

Start: 1176396

End: 1177727

Strand: Direct

Name: murD

Synonym: Saro_1131

Alternate gene names: 87199153

Gene position: 1176396-1177727 (Clockwise)

Preceding gene: 87199152

Following gene: 87199154

Centisome position: 33.03

GC content: 67.27

Gene sequence:

>1332_bases
ATGATCGTCTCGCGCGCCTTTGCCGGAAAGCGTTACGCGGTTCTCGGTCTCGCGCGCTCGGGCCTTGCCAGCGTCGAAAG
CCTGCTGGCCGGCGGCGCTGACGTCACCGTCTGGGACAACCGCGAGGAACCGCGCAGCGCATTCGAAGGGAGCTGCACCA
TCGCCGACCCGCTGTCCATCGACCTGACAGGCTTTGCGGGTGTCGTGGTTTCCCCGGGCGTGCCGCTCAACCGCCACCCC
ATCGCCGACCATGCGCGCGCCGCTGGCGTTCCGGTGATCGGTGACATCGAGTTGTTCGCCCAGGCGCGCGCCGACCTTCC
GCCACATCGCGTGGTCGGGATCACGGGCACCAACGGCAAGTCGACGACAACGATGCTGGTCCACCACATCTGCGCCGCCG
CTGGCCTTCCCGCGCGGCTCGGCGGCAATATCGGCCTGCCGATCCTCGGGCAGGAGCCCTTGCCCGAAGGCGGGGTCTAC
GTGCTCGAGCTGTCGAGCTACCAGATCGACCTGACCCAGAGCCTGGCCTGCGACGTAGCCGCGCTGATCAACCTCAGCCC
CGATCACCTTGACCGTTATGACGGCTTTGCCGGCTATGCCGCATCGAAGGCGCGCCTCTTCGCGATGCAGCGGGCCGACC
AGCACGCGGTCTTCGGCTGCGGCGACGAGCACACCCTCGAGATCGCGCGCGAGGCGTCGGCAACCCACGACACGGGCTTT
GTCCACGTCGTTCAGCCGAGTGCGCTTCACGGCCAGCTCGAATGGCCTTCGCTGCAAGGTCCGCACAACCTCCAGAACGC
CGCCATCGCGGTAGAGATCGCGCGGCAGCTTGGCATTGCCGAGGATATCTGGCGTTCAGCGCTGGCCAGCTTCGTCGGCT
TGCCGCACCGAATGGAGCGCGTGGCCGAGGCCAACGGCGTTCTCTTCGTGAACGACAGCAAGGCGACTAACCCGGCTTCC
ACCGCCCCTGCGCTCGGCGCCTATCCGCGCATCCACTGGATACTCGGCGGTCTGCCCAAGAGCGACAACCTCGACGAGTG
CGCGCCCTATTTCGATCACGTCGTTGCGGCCTATACCATCGGCGAGGCCGGGCCGCGCTTTGCCGAGATCCTCGAACCCG
TGATGCCCGTCACCCGCAGCGAGATGCTGTGCGATGCGGTGCGCCAGGCTATGGAGGCCGCGCAGCCGGGCGATGTCGTC
ATGCTGTCGCCCGCCTGCGCCAGCTTCGACCAGTTCCGCGATTTCGAGGCGCGTGGCGATTCCTTTCGGCAGATCGTCGA
GGCGCTGATCGAGGATCGGGAAGCGCCCTGTCCGGACGGAACGCCGACGTGA

Upstream 100 bases:

>100_bases
ACTTCGAGCAGCTCGGCTGGAAGGAATCGACCGTCGTCATCCGCTTCTGGATCGTGTCGATCGTGCTCGCGCTGATCGGC
CTTGCGACGCTGAAGGTTCG

Downstream 100 bases:

>100_bases
GCACCACGCCAACCCCGACGCGCCTTGTCACCACCACCATGCCCGACGGCGGCGTGAAGGTTGTCGGCCACGGTTCGCGG
TTCCGGCGCAGCAGGCGCAG

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MIVSRAFAGKRYAVLGLARSGLASVESLLAGGADVTVWDNREEPRSAFEGSCTIADPLSIDLTGFAGVVVSPGVPLNRHP
IADHARAAGVPVIGDIELFAQARADLPPHRVVGITGTNGKSTTTMLVHHICAAAGLPARLGGNIGLPILGQEPLPEGGVY
VLELSSYQIDLTQSLACDVAALINLSPDHLDRYDGFAGYAASKARLFAMQRADQHAVFGCGDEHTLEIAREASATHDTGF
VHVVQPSALHGQLEWPSLQGPHNLQNAAIAVEIARQLGIAEDIWRSALASFVGLPHRMERVAEANGVLFVNDSKATNPAS
TAPALGAYPRIHWILGGLPKSDNLDECAPYFDHVVAAYTIGEAGPRFAEILEPVMPVTRSEMLCDAVRQAMEAAQPGDVV
MLSPACASFDQFRDFEARGDSFRQIVEALIEDREAPCPDGTPT

Sequences:

>Translated_443_residues
MIVSRAFAGKRYAVLGLARSGLASVESLLAGGADVTVWDNREEPRSAFEGSCTIADPLSIDLTGFAGVVVSPGVPLNRHP
IADHARAAGVPVIGDIELFAQARADLPPHRVVGITGTNGKSTTTMLVHHICAAAGLPARLGGNIGLPILGQEPLPEGGVY
VLELSSYQIDLTQSLACDVAALINLSPDHLDRYDGFAGYAASKARLFAMQRADQHAVFGCGDEHTLEIAREASATHDTGF
VHVVQPSALHGQLEWPSLQGPHNLQNAAIAVEIARQLGIAEDIWRSALASFVGLPHRMERVAEANGVLFVNDSKATNPAS
TAPALGAYPRIHWILGGLPKSDNLDECAPYFDHVVAAYTIGEAGPRFAEILEPVMPVTRSEMLCDAVRQAMEAAQPGDVV
MLSPACASFDQFRDFEARGDSFRQIVEALIEDREAPCPDGTPT
>Mature_443_residues
MIVSRAFAGKRYAVLGLARSGLASVESLLAGGADVTVWDNREEPRSAFEGSCTIADPLSIDLTGFAGVVVSPGVPLNRHP
IADHARAAGVPVIGDIELFAQARADLPPHRVVGITGTNGKSTTTMLVHHICAAAGLPARLGGNIGLPILGQEPLPEGGVY
VLELSSYQIDLTQSLACDVAALINLSPDHLDRYDGFAGYAASKARLFAMQRADQHAVFGCGDEHTLEIAREASATHDTGF
VHVVQPSALHGQLEWPSLQGPHNLQNAAIAVEIARQLGIAEDIWRSALASFVGLPHRMERVAEANGVLFVNDSKATNPAS
TAPALGAYPRIHWILGGLPKSDNLDECAPYFDHVVAAYTIGEAGPRFAEILEPVMPVTRSEMLCDAVRQAMEAAQPGDVV
MLSPACASFDQFRDFEARGDSFRQIVEALIEDREAPCPDGTPT

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=458, Percent_Identity=36.8995633187773, Blast_Score=209, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_NOVAD (Q2G997)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_496410.1
- ProteinModelPortal:   Q2G997
- SMR:   Q2G997
- STRING:   Q2G997
- GeneID:   3916427
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_1131
- NMPDR:   fig|48935.1.peg.663
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- PhylomeDB:   Q2G997
- ProtClustDB:   CLSK835273
- BioCyc:   NARO279238:SARO_1131-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 47012; Mature: 47012

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00626 RCC1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVSRAFAGKRYAVLGLARSGLASVESLLAGGADVTVWDNREEPRSAFEGSCTIADPLSI
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCCEECCCCEE
DLTGFAGVVVSPGVPLNRHPIADHARAAGVPVIGDIELFAQARADLPPHRVVGITGTNGK
EECCCCCEEECCCCCCCCCCCHHHHHHCCCCEEECHHHHHHHHCCCCCCEEEEEECCCCC
STTTMLVHHICAAAGLPARLGGNIGLPILGQEPLPEGGVYVLELSSYQIDLTQSLACDVA
CHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCEEEEEECCEEEEHHHHHHHHHH
ALINLSPDHLDRYDGFAGYAASKARLFAMQRADQHAVFGCGDEHTLEIAREASATHDTGF
HHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCCCCE
VHVVQPSALHGQLEWPSLQGPHNLQNAAIAVEIARQLGIAEDIWRSALASFVGLPHRMER
EEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHH
VAEANGVLFVNDSKATNPASTAPALGAYPRIHWILGGLPKSDNLDECAPYFDHVVAAYTI
HHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
GEAGPRFAEILEPVMPVTRSEMLCDAVRQAMEAAQPGDVVMLSPACASFDQFRDFEARGD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHCHHHHHHHHHCCH
SFRQIVEALIEDREAPCPDGTPT
HHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MIVSRAFAGKRYAVLGLARSGLASVESLLAGGADVTVWDNREEPRSAFEGSCTIADPLSI
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCCEECCCCEE
DLTGFAGVVVSPGVPLNRHPIADHARAAGVPVIGDIELFAQARADLPPHRVVGITGTNGK
EECCCCCEEECCCCCCCCCCCHHHHHHCCCCEEECHHHHHHHHCCCCCCEEEEEECCCCC
STTTMLVHHICAAAGLPARLGGNIGLPILGQEPLPEGGVYVLELSSYQIDLTQSLACDVA
CHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCEEEEEECCEEEEHHHHHHHHHH
ALINLSPDHLDRYDGFAGYAASKARLFAMQRADQHAVFGCGDEHTLEIAREASATHDTGF
HHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCCCCE
VHVVQPSALHGQLEWPSLQGPHNLQNAAIAVEIARQLGIAEDIWRSALASFVGLPHRMER
EEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHH
VAEANGVLFVNDSKATNPASTAPALGAYPRIHWILGGLPKSDNLDECAPYFDHVVAAYTI
HHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
GEAGPRFAEILEPVMPVTRSEMLCDAVRQAMEAAQPGDVVMLSPACASFDQFRDFEARGD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHCHHHHHHHHHCCH
SFRQIVEALIEDREAPCPDGTPT
HHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA