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The map label for this gene is tolB

Identifier: 87199039

GI number: 87199039

Start: 1056470

End: 1057855

Strand: Direct

Name: tolB

Synonym: Saro_1017

Alternate gene names: 87199039

Gene position: 1056470-1057855 (Clockwise)

Preceding gene: 87199038

Following gene: 87199040

Centisome position: 29.66

GC content: 65.95

Gene sequence:

>1386_bases
ATGAAGATCCGTCACCTTCTCCTGCTAGCGGGGCTGGTCTCCGCGCCAGCCATCGTCGCGGCCCAGCAGACCGCATTGCC
GTCGTCTAGCGCGGCGCAGGCATCGGGCGACGACGACGGTGGCCTGACCGGCTCTGTTTCCGACGAAAGCGAGTGGCAGG
ACCTCGGCATCGCCATTCCCTCGTTCCCGACCAACGCTTCGGTCCCGACCGCGGCCGAAGGCGGGACCACCGAGGCGTTG
GGGCGGAACGTCGCGCGCGTGGTATTCAACGATCTCAAGAACAACGGCCTGTTCAAGCCGGTCGGCCCGGATGCGCTTCC
CGCCATCGCCTTTCCGCAGGTGGCCGCGCCCGCGTTCGACGCCTGGCGCGGCCGTTCGGCCGAAATGCTGGTGCAGGGCT
TCGTCAAGGCGAACGAGGACGGCCGCCTGACGGTCGGCTGCTATCTCTACGACGTCGCGCTCGGCAGTGAACTGGCGCGG
CAGGGCTATGTCGTGAAGCCGGAGGAATGGCGCCGCGCCGCGCACAAGTGCGCTGACATGGTCTATTCGCGCCTGTCCGG
CGAAAGCCCGTTCTTCGACAGCAAGATCGCCTATATCGCCGAGACCGGGCCCAAGGACCGCCGCCGCAAGCAACTGGCGA
TCATGGATTCCGATGGCGCCAACCATCGCTTCATCACCAATGGCCAGGCGACCGCGCTGACGCCGCGCTATTCGCCGGAC
TACAGCCAGATTGTCTACCTCAGCTATCTGAACGGCGCGCCGCGCATCTACATCTACAACATCGGCACCGGCCAGCAGAA
GCTCGTCACGTCGAGCACCAACCCAACCTTCGCCCCACGCTGGTCGCCCGACGGCAAGTGGATTCTCTATTCGATGTCTG
TCGCGGGCAACACCGACATCTATCGCGTCTCGGTCAACGGCGGCGCCTCGACGCGTCTGACCGACAGCCCGGGCATCGAC
GTCGGCGGGTCCTATTCGCCCGATGGCAGCAGGATCGTGTTCGAAAGCGACCGTTCGGGCAGCCAGCAACTCTACGTGAT
GAACGCGGATGGATCGAACCAGAAGCGCATCAGCTTCTTCGGTGGCCGCGCCGCGACGCCCGAATGGTCCCCGCGCGGCG
ACCAGATCGCCTTCACGCATCTCGGCGGCAATTTCCGCATCGCGGTGACCGATCCCTCGGGCGGCAACATGCGCTTCCTC
ACCGATAGCTGGCAGGACGAGGCGCCCACCTGGTCGCCCAACGGCCGCATCGTCCAGTTCTTCCGGACCGAGCGCGGCAG
CGGCAAGACCGGCATCTGGCAGGTTGACCTCACCGGCCGGAACGAGCGGAAACTGAACACGCCCGTCGATGGTTCCGACC
CTGCATGGGGACCCGTCCTGCCCTGA

Upstream 100 bases:

>100_bases
TACGTGCCGTTCAGCTCGCCGCGCCCTTCGAACTCCCCGCAATGTACTATTCCGGCTGGAAGCGCGTCGCCGCCTTCCGC
TTTGACAGGAAGTTGTCCCA

Downstream 100 bases:

>100_bases
ACGCGGTCGTAAACCGATCCGGTGCAAACGTCGTGGCCATGGAGCTTGTCATTTCATCGCCGGATATGCACGATACGTCG
CCCCTCTCCCAATCCCTCCC

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MKIRHLLLLAGLVSAPAIVAAQQTALPSSSAAQASGDDDGGLTGSVSDESEWQDLGIAIPSFPTNASVPTAAEGGTTEAL
GRNVARVVFNDLKNNGLFKPVGPDALPAIAFPQVAAPAFDAWRGRSAEMLVQGFVKANEDGRLTVGCYLYDVALGSELAR
QGYVVKPEEWRRAAHKCADMVYSRLSGESPFFDSKIAYIAETGPKDRRRKQLAIMDSDGANHRFITNGQATALTPRYSPD
YSQIVYLSYLNGAPRIYIYNIGTGQQKLVTSSTNPTFAPRWSPDGKWILYSMSVAGNTDIYRVSVNGGASTRLTDSPGID
VGGSYSPDGSRIVFESDRSGSQQLYVMNADGSNQKRISFFGGRAATPEWSPRGDQIAFTHLGGNFRIAVTDPSGGNMRFL
TDSWQDEAPTWSPNGRIVQFFRTERGSGKTGIWQVDLTGRNERKLNTPVDGSDPAWGPVLP

Sequences:

>Translated_461_residues
MKIRHLLLLAGLVSAPAIVAAQQTALPSSSAAQASGDDDGGLTGSVSDESEWQDLGIAIPSFPTNASVPTAAEGGTTEAL
GRNVARVVFNDLKNNGLFKPVGPDALPAIAFPQVAAPAFDAWRGRSAEMLVQGFVKANEDGRLTVGCYLYDVALGSELAR
QGYVVKPEEWRRAAHKCADMVYSRLSGESPFFDSKIAYIAETGPKDRRRKQLAIMDSDGANHRFITNGQATALTPRYSPD
YSQIVYLSYLNGAPRIYIYNIGTGQQKLVTSSTNPTFAPRWSPDGKWILYSMSVAGNTDIYRVSVNGGASTRLTDSPGID
VGGSYSPDGSRIVFESDRSGSQQLYVMNADGSNQKRISFFGGRAATPEWSPRGDQIAFTHLGGNFRIAVTDPSGGNMRFL
TDSWQDEAPTWSPNGRIVQFFRTERGSGKTGIWQVDLTGRNERKLNTPVDGSDPAWGPVLP
>Mature_461_residues
MKIRHLLLLAGLVSAPAIVAAQQTALPSSSAAQASGDDDGGLTGSVSDESEWQDLGIAIPSFPTNASVPTAAEGGTTEAL
GRNVARVVFNDLKNNGLFKPVGPDALPAIAFPQVAAPAFDAWRGRSAEMLVQGFVKANEDGRLTVGCYLYDVALGSELAR
QGYVVKPEEWRRAAHKCADMVYSRLSGESPFFDSKIAYIAETGPKDRRRKQLAIMDSDGANHRFITNGQATALTPRYSPD
YSQIVYLSYLNGAPRIYIYNIGTGQQKLVTSSTNPTFAPRWSPDGKWILYSMSVAGNTDIYRVSVNGGASTRLTDSPGID
VGGSYSPDGSRIVFESDRSGSQQLYVMNADGSNQKRISFFGGRAATPEWSPRGDQIAFTHLGGNFRIAVTDPSGGNMRFL
TDSWQDEAPTWSPNGRIVQFFRTERGSGKTGIWQVDLTGRNERKLNTPVDGSDPAWGPVLP

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=385, Percent_Identity=28.8311688311688, Blast_Score=149, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI32564215, Length=89, Percent_Identity=39.3258426966292, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI32564217, Length=89, Percent_Identity=39.3258426966292, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB2_NOVAD (Q2G9L1)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_496296.1
- ProteinModelPortal:   Q2G9L1
- SMR:   Q2G9L1
- STRING:   Q2G9L1
- GeneID:   3915799
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_1017
- NMPDR:   fig|48935.1.peg.3121
- eggNOG:   COG0823
- HOGENOM:   HBG644919
- OMA:   FCTRIAY
- PhylomeDB:   Q2G9L1
- ProtClustDB:   PRK05137
- BioCyc:   NARO279238:SARO_1017-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 49703; Mature: 49703

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRHLLLLAGLVSAPAIVAAQQTALPSSSAAQASGDDDGGLTGSVSDESEWQDLGIAIP
CCHHHHHHHHHHHCCCEEEEEHHHCCCCCCCCCCCCCCCCCEEECCCCCCCHHHCCEECC
SFPTNASVPTAAEGGTTEALGRNVARVVFNDLKNNGLFKPVGPDALPAIAFPQVAAPAFD
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHH
AWRGRSAEMLVQGFVKANEDGRLTVGCYLYDVALGSELARQGYVVKPEEWRRAAHKCADM
HHCCCCHHHHHHHHHCCCCCCCEEEEEEEEEEHHHHHHHHCCEEECCHHHHHHHHHHHHH
VYSRLSGESPFFDSKIAYIAETGPKDRRRKQLAIMDSDGANHRFITNGQATALTPRYSPD
HHHHHCCCCCCCCCEEEEEECCCCCHHCCCEEEEEECCCCCCEEEECCCEEEECCCCCCC
YSQIVYLSYLNGAPRIYIYNIGTGQQKLVTSSTNPTFAPRWSPDGKWILYSMSVAGNTDI
HHEEEEEEEECCCCEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCEE
YRVSVNGGASTRLTDSPGIDVGGSYSPDGSRIVFESDRSGSQQLYVMNADGSNQKRISFF
EEEEECCCCCEEECCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCEEEEEE
GGRAATPEWSPRGDQIAFTHLGGNFRIAVTDPSGGNMRFLTDSWQDEAPTWSPNGRIVQF
CCCCCCCCCCCCCCEEEEEEECCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCEEEE
FRTERGSGKTGIWQVDLTGRNERKLNTPVDGSDPAWGPVLP
EECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIRHLLLLAGLVSAPAIVAAQQTALPSSSAAQASGDDDGGLTGSVSDESEWQDLGIAIP
CCHHHHHHHHHHHCCCEEEEEHHHCCCCCCCCCCCCCCCCCEEECCCCCCCHHHCCEECC
SFPTNASVPTAAEGGTTEALGRNVARVVFNDLKNNGLFKPVGPDALPAIAFPQVAAPAFD
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHH
AWRGRSAEMLVQGFVKANEDGRLTVGCYLYDVALGSELARQGYVVKPEEWRRAAHKCADM
HHCCCCHHHHHHHHHCCCCCCCEEEEEEEEEEHHHHHHHHCCEEECCHHHHHHHHHHHHH
VYSRLSGESPFFDSKIAYIAETGPKDRRRKQLAIMDSDGANHRFITNGQATALTPRYSPD
HHHHHCCCCCCCCCEEEEEECCCCCHHCCCEEEEEECCCCCCEEEECCCEEEECCCCCCC
YSQIVYLSYLNGAPRIYIYNIGTGQQKLVTSSTNPTFAPRWSPDGKWILYSMSVAGNTDI
HHEEEEEEEECCCCEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCEE
YRVSVNGGASTRLTDSPGIDVGGSYSPDGSRIVFESDRSGSQQLYVMNADGSNQKRISFF
EEEEECCCCCEEECCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCEEEEEE
GGRAATPEWSPRGDQIAFTHLGGNFRIAVTDPSGGNMRFLTDSWQDEAPTWSPNGRIVQF
CCCCCCCCCCCCCCEEEEEEECCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCEEEE
FRTERGSGKTGIWQVDLTGRNERKLNTPVDGSDPAWGPVLP
EECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA